Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queA and tgt are separated by 14 nucleotides tgt and yajC are separated by 47 nucleotides yajC and secD are separated by 63 nucleotides
COO64_RS03845: queA - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 242,128 to 243,177
queA
COO64_RS03850: tgt - tRNA guanosine(34) transglycosylase Tgt, at 243,192 to 244,307
tgt
COO64_RS03855: yajC - preprotein translocase subunit YajC, at 244,355 to 244,690
yajC
COO64_RS03860: secD - protein translocase subunit SecD, at 244,754 to 246,619
secD
Position (kb) 243
244
245 Strain fitness (log2 ratio) -2
-1
0
1
2 at 242.199 kb on + strand at 242.199 kb on + strand at 242.199 kb on + strand at 242.200 kb on - strand at 242.200 kb on - strand at 242.381 kb on + strand, within queA at 242.553 kb on + strand, within queA at 242.553 kb on + strand, within queA at 242.553 kb on + strand, within queA at 242.553 kb on + strand, within queA at 242.570 kb on - strand, within queA at 242.618 kb on + strand, within queA at 242.618 kb on + strand, within queA at 242.618 kb on + strand, within queA at 242.618 kb on + strand, within queA at 242.618 kb on + strand, within queA at 242.618 kb on + strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.619 kb on - strand, within queA at 242.620 kb on + strand, within queA at 242.620 kb on + strand, within queA at 242.620 kb on + strand, within queA at 242.620 kb on + strand, within queA at 242.620 kb on + strand, within queA at 242.620 kb on + strand, within queA at 242.620 kb on + strand, within queA at 242.621 kb on - strand, within queA at 242.621 kb on - strand, within queA at 242.621 kb on - strand, within queA at 242.745 kb on + strand, within queA at 242.746 kb on - strand, within queA at 242.746 kb on - strand, within queA at 242.793 kb on + strand, within queA at 242.793 kb on + strand, within queA at 242.793 kb on + strand, within queA at 242.793 kb on + strand, within queA at 242.794 kb on - strand, within queA at 242.949 kb on + strand, within queA at 242.949 kb on + strand, within queA at 242.949 kb on + strand, within queA at 242.950 kb on - strand, within queA at 242.950 kb on - strand, within queA at 242.955 kb on + strand, within queA at 242.955 kb on + strand, within queA at 242.956 kb on - strand, within queA at 242.956 kb on - strand, within queA at 242.956 kb on - strand, within queA at 243.076 kb on + strand at 243.076 kb on + strand at 243.115 kb on + strand at 243.115 kb on + strand at 243.115 kb on + strand at 243.116 kb on - strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.191 kb on + strand at 243.192 kb on - strand at 243.192 kb on - strand at 243.192 kb on - strand at 243.192 kb on - strand at 243.192 kb on - strand at 243.294 kb on + strand at 243.294 kb on + strand at 243.295 kb on - strand at 243.295 kb on - strand at 243.362 kb on + strand, within tgt at 243.362 kb on + strand, within tgt at 243.362 kb on + strand, within tgt at 243.362 kb on + strand, within tgt at 243.363 kb on - strand, within tgt at 243.771 kb on - strand, within tgt at 243.771 kb on - strand, within tgt at 243.873 kb on + strand, within tgt at 243.873 kb on + strand, within tgt at 243.873 kb on + strand, within tgt at 243.909 kb on + strand, within tgt at 243.915 kb on - strand, within tgt at 243.915 kb on - strand, within tgt at 243.922 kb on + strand, within tgt at 244.098 kb on + strand, within tgt at 244.098 kb on + strand, within tgt at 244.098 kb on + strand, within tgt at 244.098 kb on + strand, within tgt at 244.099 kb on - strand, within tgt at 244.099 kb on - strand, within tgt at 244.099 kb on - strand, within tgt at 244.099 kb on - strand, within tgt at 244.099 kb on - strand, within tgt at 244.099 kb on - strand, within tgt at 244.100 kb on + strand, within tgt at 244.100 kb on + strand, within tgt at 244.100 kb on + strand, within tgt at 244.101 kb on - strand, within tgt at 244.101 kb on - strand, within tgt at 244.101 kb on - strand, within tgt at 244.125 kb on + strand, within tgt at 244.125 kb on + strand, within tgt at 244.126 kb on - strand, within tgt at 244.126 kb on - strand, within tgt at 244.126 kb on - strand, within tgt at 244.126 kb on - strand, within tgt at 244.143 kb on - strand, within tgt at 244.166 kb on + strand, within tgt at 244.166 kb on + strand, within tgt at 244.166 kb on + strand, within tgt at 244.167 kb on - strand, within tgt at 244.167 kb on - strand, within tgt at 244.175 kb on + strand, within tgt at 244.175 kb on + strand, within tgt at 244.176 kb on - strand, within tgt at 244.176 kb on - strand, within tgt at 244.184 kb on + strand, within tgt at 244.185 kb on - strand, within tgt at 244.198 kb on - strand at 244.241 kb on + strand at 244.241 kb on + strand at 244.241 kb on + strand at 244.241 kb on + strand at 244.241 kb on + strand at 244.269 kb on + strand at 244.269 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.310 kb on + strand at 244.323 kb on + strand at 244.330 kb on + strand at 244.330 kb on + strand at 244.352 kb on + strand at 244.379 kb on + strand at 244.463 kb on + strand, within yajC at 244.666 kb on + strand at 244.688 kb on + strand at 244.688 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 242,199 + -1.0 242,199 + +1.6 242,199 + +0.1 242,200 - -1.7 242,200 - -1.0 242,381 + queA COO64_RS03845 0.24 -0.5 242,553 + queA COO64_RS03845 0.40 -0.9 242,553 + queA COO64_RS03845 0.40 -1.0 242,553 + queA COO64_RS03845 0.40 -0.1 242,553 + queA COO64_RS03845 0.40 -1.2 242,570 - queA COO64_RS03845 0.42 -0.7 242,618 + queA COO64_RS03845 0.47 -0.4 242,618 + queA COO64_RS03845 0.47 -1.4 242,618 + queA COO64_RS03845 0.47 -0.9 242,618 + queA COO64_RS03845 0.47 -0.3 242,618 + queA COO64_RS03845 0.47 -1.4 242,618 + queA COO64_RS03845 0.47 +2.4 242,619 - queA COO64_RS03845 0.47 +0.0 242,619 - queA COO64_RS03845 0.47 -1.6 242,619 - queA COO64_RS03845 0.47 +0.8 242,619 - queA COO64_RS03845 0.47 +0.4 242,619 - queA COO64_RS03845 0.47 -2.6 242,619 - queA COO64_RS03845 0.47 -0.8 242,619 - queA COO64_RS03845 0.47 -0.8 242,619 - queA COO64_RS03845 0.47 -1.9 242,619 - queA COO64_RS03845 0.47 -1.0 242,619 - queA COO64_RS03845 0.47 -2.4 242,619 - queA COO64_RS03845 0.47 -1.6 242,620 + queA COO64_RS03845 0.47 +0.1 242,620 + queA COO64_RS03845 0.47 +0.2 242,620 + queA COO64_RS03845 0.47 -1.3 242,620 + queA COO64_RS03845 0.47 +2.0 242,620 + queA COO64_RS03845 0.47 -0.6 242,620 + queA COO64_RS03845 0.47 -0.1 242,620 + queA COO64_RS03845 0.47 +0.3 242,621 - queA COO64_RS03845 0.47 -0.8 242,621 - queA COO64_RS03845 0.47 +0.0 242,621 - queA COO64_RS03845 0.47 -1.0 242,745 + queA COO64_RS03845 0.59 +0.5 242,746 - queA COO64_RS03845 0.59 +1.1 242,746 - queA COO64_RS03845 0.59 -0.8 242,793 + queA COO64_RS03845 0.63 -0.3 242,793 + queA COO64_RS03845 0.63 +0.6 242,793 + queA COO64_RS03845 0.63 +0.2 242,793 + queA COO64_RS03845 0.63 -0.9 242,794 - queA COO64_RS03845 0.63 -1.4 242,949 + queA COO64_RS03845 0.78 +0.4 242,949 + queA COO64_RS03845 0.78 -0.3 242,949 + queA COO64_RS03845 0.78 -0.3 242,950 - queA COO64_RS03845 0.78 -0.4 242,950 - queA COO64_RS03845 0.78 -0.1 242,955 + queA COO64_RS03845 0.79 +0.3 242,955 + queA COO64_RS03845 0.79 +0.5 242,956 - queA COO64_RS03845 0.79 +1.2 242,956 - queA COO64_RS03845 0.79 +0.0 242,956 - queA COO64_RS03845 0.79 +0.0 243,076 + +0.8 243,076 + -0.4 243,115 + -0.0 243,115 + -0.7 243,115 + +0.7 243,116 - +0.3 243,191 + +0.3 243,191 + -0.3 243,191 + +0.8 243,191 + +2.0 243,191 + -1.8 243,191 + -0.0 243,191 + -0.6 243,191 + -0.3 243,192 - +2.4 243,192 - +0.3 243,192 - +1.0 243,192 - +2.3 243,192 - -1.9 243,294 + -0.6 243,294 + +1.3 243,295 - -0.6 243,295 - -0.8 243,362 + tgt COO64_RS03850 0.15 -1.1 243,362 + tgt COO64_RS03850 0.15 -0.6 243,362 + tgt COO64_RS03850 0.15 +0.6 243,362 + tgt COO64_RS03850 0.15 -1.7 243,363 - tgt COO64_RS03850 0.15 -0.1 243,771 - tgt COO64_RS03850 0.52 +0.4 243,771 - tgt COO64_RS03850 0.52 -0.7 243,873 + tgt COO64_RS03850 0.61 +0.4 243,873 + tgt COO64_RS03850 0.61 -1.3 243,873 + tgt COO64_RS03850 0.61 +0.6 243,909 + tgt COO64_RS03850 0.64 -1.6 243,915 - tgt COO64_RS03850 0.65 -0.3 243,915 - tgt COO64_RS03850 0.65 -1.1 243,922 + tgt COO64_RS03850 0.65 -0.9 244,098 + tgt COO64_RS03850 0.81 +0.5 244,098 + tgt COO64_RS03850 0.81 +0.9 244,098 + tgt COO64_RS03850 0.81 -0.3 244,098 + tgt COO64_RS03850 0.81 -0.4 244,099 - tgt COO64_RS03850 0.81 -0.5 244,099 - tgt COO64_RS03850 0.81 -2.2 244,099 - tgt COO64_RS03850 0.81 -1.3 244,099 - tgt COO64_RS03850 0.81 -0.6 244,099 - tgt COO64_RS03850 0.81 +0.0 244,099 - tgt COO64_RS03850 0.81 -0.8 244,100 + tgt COO64_RS03850 0.81 +0.6 244,100 + tgt COO64_RS03850 0.81 +0.6 244,100 + tgt COO64_RS03850 0.81 +0.0 244,101 - tgt COO64_RS03850 0.81 +0.7 244,101 - tgt COO64_RS03850 0.81 -0.3 244,101 - tgt COO64_RS03850 0.81 -0.7 244,125 + tgt COO64_RS03850 0.84 +0.4 244,125 + tgt COO64_RS03850 0.84 +0.9 244,126 - tgt COO64_RS03850 0.84 +0.4 244,126 - tgt COO64_RS03850 0.84 -0.8 244,126 - tgt COO64_RS03850 0.84 -0.4 244,126 - tgt COO64_RS03850 0.84 -0.8 244,143 - tgt COO64_RS03850 0.85 -0.3 244,166 + tgt COO64_RS03850 0.87 +1.3 244,166 + tgt COO64_RS03850 0.87 -1.9 244,166 + tgt COO64_RS03850 0.87 -0.6 244,167 - tgt COO64_RS03850 0.87 -0.7 244,167 - tgt COO64_RS03850 0.87 -1.0 244,175 + tgt COO64_RS03850 0.88 +1.7 244,175 + tgt COO64_RS03850 0.88 -1.1 244,176 - tgt COO64_RS03850 0.88 +0.0 244,176 - tgt COO64_RS03850 0.88 -1.1 244,184 + tgt COO64_RS03850 0.89 -0.9 244,185 - tgt COO64_RS03850 0.89 +0.2 244,198 - +1.2 244,241 + -0.9 244,241 + +0.6 244,241 + +0.4 244,241 + -0.4 244,241 + +0.6 244,269 + +0.2 244,269 + -0.4 244,310 + -0.7 244,310 + +0.4 244,310 + -0.2 244,310 + -1.2 244,310 + -0.7 244,310 + -0.4 244,310 + +0.3 244,310 + -0.1 244,310 + +1.3 244,310 + -0.8 244,310 + +0.2 244,310 + +0.8 244,310 + -0.6 244,310 + -0.5 244,310 + +1.0 244,310 + -0.7 244,323 + -0.1 244,330 + +0.2 244,330 + +0.3 244,352 + +1.0 244,379 + +0.8 244,463 + yajC COO64_RS03855 0.32 -1.5 244,666 + -0.8 244,688 + +0.0 244,688 + +0.5
Or see this region's nucleotide sequence