Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alaC and COO64_RS03730 are separated by 180 nucleotides COO64_RS03730 and COO64_RS03735 are separated by 54 nucleotides COO64_RS03735 and COO64_RS03740 are separated by 69 nucleotides COO64_RS03740 and COO64_RS03745 overlap by 1 nucleotides
COO64_RS03725: alaC - alanine transaminase, at 223,794 to 225,014
alaC
COO64_RS03730: COO64_RS03730 - YkgJ family cysteine cluster protein, at 225,195 to 225,569
_RS03730
COO64_RS03735: COO64_RS03735 - GNAT family N-acetyltransferase, at 225,624 to 226,193
_RS03735
COO64_RS03740: COO64_RS03740 - hypothetical protein, at 226,263 to 226,790
_RS03740
COO64_RS03745: COO64_RS03745 - hypothetical protein, at 226,790 to 227,341
_RS03745
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 224.778 kb on + strand, within alaC at 224.778 kb on + strand, within alaC at 224.778 kb on + strand, within alaC at 224.778 kb on + strand, within alaC at 224.778 kb on + strand, within alaC at 224.778 kb on + strand, within alaC at 224.779 kb on - strand, within alaC at 224.779 kb on - strand, within alaC at 224.779 kb on - strand, within alaC at 224.779 kb on - strand, within alaC at 224.779 kb on - strand, within alaC at 224.779 kb on - strand, within alaC at 224.779 kb on - strand, within alaC at 224.806 kb on - strand, within alaC at 224.892 kb on + strand, within alaC at 224.893 kb on - strand at 225.012 kb on + strand at 225.012 kb on + strand at 225.012 kb on + strand at 225.013 kb on - strand at 225.013 kb on - strand at 225.018 kb on + strand at 225.018 kb on + strand at 225.018 kb on + strand at 225.018 kb on + strand at 225.018 kb on + strand at 225.019 kb on - strand at 225.019 kb on - strand at 225.019 kb on - strand at 225.019 kb on - strand at 225.047 kb on + strand at 225.099 kb on + strand at 225.100 kb on - strand at 225.100 kb on - strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.140 kb on + strand at 225.141 kb on - strand at 225.141 kb on - strand at 225.141 kb on - strand at 225.141 kb on - strand at 225.141 kb on - strand at 225.163 kb on + strand at 225.164 kb on - strand at 225.164 kb on - strand at 225.164 kb on - strand at 225.165 kb on + strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.206 kb on + strand at 225.206 kb on + strand at 225.206 kb on + strand at 225.206 kb on + strand at 225.207 kb on - strand at 225.207 kb on - strand at 225.207 kb on - strand at 225.240 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.414 kb on + strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.415 kb on - strand, within COO64_RS03730 at 225.586 kb on + strand at 225.587 kb on - strand at 225.587 kb on - strand at 225.587 kb on - strand at 225.587 kb on - strand at 225.636 kb on + strand at 225.636 kb on + strand at 225.636 kb on + strand at 225.636 kb on + strand at 225.636 kb on + strand at 225.636 kb on + strand at 225.637 kb on - strand at 225.637 kb on - strand at 225.637 kb on - strand at 225.637 kb on - strand at 225.637 kb on - strand at 225.637 kb on - strand at 225.819 kb on + strand, within COO64_RS03735 at 225.819 kb on + strand, within COO64_RS03735 at 225.819 kb on + strand, within COO64_RS03735 at 225.820 kb on - strand, within COO64_RS03735 at 225.820 kb on - strand, within COO64_RS03735 at 225.999 kb on + strand, within COO64_RS03735 at 225.999 kb on + strand, within COO64_RS03735 at 226.000 kb on - strand, within COO64_RS03735 at 226.153 kb on + strand at 226.153 kb on + strand at 226.153 kb on + strand at 226.153 kb on + strand at 226.154 kb on - strand at 226.154 kb on - strand at 226.155 kb on + strand at 226.156 kb on - strand at 226.156 kb on - strand at 226.156 kb on - strand at 226.195 kb on + strand at 226.195 kb on + strand at 226.196 kb on - strand at 226.196 kb on - strand at 226.223 kb on + strand at 226.223 kb on + strand at 226.224 kb on - strand at 226.224 kb on - strand at 226.258 kb on + strand at 226.259 kb on - strand at 226.275 kb on + strand at 226.275 kb on + strand at 226.275 kb on + strand at 226.276 kb on - strand at 226.299 kb on + strand at 226.461 kb on + strand, within COO64_RS03740 at 226.462 kb on - strand, within COO64_RS03740 at 226.462 kb on - strand, within COO64_RS03740 at 226.462 kb on - strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.572 kb on + strand, within COO64_RS03740 at 226.573 kb on + strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.573 kb on - strand, within COO64_RS03740 at 226.640 kb on + strand, within COO64_RS03740 at 226.641 kb on - strand, within COO64_RS03740 at 226.696 kb on + strand, within COO64_RS03740 at 226.697 kb on - strand, within COO64_RS03740 at 226.697 kb on - strand, within COO64_RS03740 at 226.697 kb on - strand, within COO64_RS03740 at 226.730 kb on + strand, within COO64_RS03740 at 226.731 kb on - strand, within COO64_RS03740 at 226.731 kb on - strand, within COO64_RS03740 at 226.731 kb on - strand, within COO64_RS03740 at 226.763 kb on - strand at 226.763 kb on - strand at 226.802 kb on + strand at 226.803 kb on - strand at 226.826 kb on + strand at 226.826 kb on + strand at 226.826 kb on + strand at 226.856 kb on + strand, within COO64_RS03745 at 226.856 kb on + strand, within COO64_RS03745 at 226.856 kb on + strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.857 kb on - strand, within COO64_RS03745 at 226.932 kb on - strand, within COO64_RS03745 at 226.932 kb on - strand, within COO64_RS03745 at 226.932 kb on - strand, within COO64_RS03745 at 226.932 kb on - strand, within COO64_RS03745 at 227.024 kb on + strand, within COO64_RS03745 at 227.024 kb on + strand, within COO64_RS03745 at 227.025 kb on - strand, within COO64_RS03745 at 227.025 kb on - strand, within COO64_RS03745 at 227.076 kb on - strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.114 kb on + strand, within COO64_RS03745 at 227.115 kb on - strand, within COO64_RS03745 at 227.115 kb on - strand, within COO64_RS03745 at 227.115 kb on - strand, within COO64_RS03745 at 227.115 kb on - strand, within COO64_RS03745 at 227.115 kb on - strand, within COO64_RS03745
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 224,778 + alaC COO64_RS03725 0.81 +0.3 224,778 + alaC COO64_RS03725 0.81 +0.1 224,778 + alaC COO64_RS03725 0.81 -1.1 224,778 + alaC COO64_RS03725 0.81 +0.3 224,778 + alaC COO64_RS03725 0.81 -0.4 224,778 + alaC COO64_RS03725 0.81 +1.4 224,779 - alaC COO64_RS03725 0.81 -0.8 224,779 - alaC COO64_RS03725 0.81 -0.4 224,779 - alaC COO64_RS03725 0.81 +0.7 224,779 - alaC COO64_RS03725 0.81 -0.9 224,779 - alaC COO64_RS03725 0.81 -0.3 224,779 - alaC COO64_RS03725 0.81 -0.7 224,779 - alaC COO64_RS03725 0.81 -0.9 224,806 - alaC COO64_RS03725 0.83 +0.6 224,892 + alaC COO64_RS03725 0.90 -0.2 224,893 - -0.4 225,012 + -0.1 225,012 + -0.3 225,012 + +0.7 225,013 - -0.3 225,013 - +0.3 225,018 + -1.0 225,018 + -0.7 225,018 + -0.7 225,018 + +0.5 225,018 + -0.1 225,019 - +0.3 225,019 - -0.8 225,019 - +1.0 225,019 - -0.3 225,047 + +0.5 225,099 + +0.8 225,100 - +0.5 225,100 - -0.8 225,140 + +0.4 225,140 + +1.8 225,140 + -0.9 225,140 + +0.9 225,140 + +0.2 225,140 + +0.7 225,140 + +0.1 225,140 + +0.7 225,140 + -0.1 225,140 + -0.8 225,141 - -0.3 225,141 - +0.2 225,141 - +0.3 225,141 - +0.1 225,141 - +0.4 225,163 + -0.2 225,164 - -1.8 225,164 - -0.0 225,164 - +0.1 225,165 + +0.5 225,166 - +0.3 225,166 - +0.6 225,206 + -0.7 225,206 + +0.1 225,206 + +0.3 225,206 + -0.8 225,207 - -1.9 225,207 - +0.6 225,207 - +0.4 225,240 + COO64_RS03730 0.12 +0.8 225,414 + COO64_RS03730 0.58 +0.9 225,414 + COO64_RS03730 0.58 -0.1 225,414 + COO64_RS03730 0.58 +0.5 225,414 + COO64_RS03730 0.58 +0.1 225,414 + COO64_RS03730 0.58 -0.4 225,414 + COO64_RS03730 0.58 +1.4 225,414 + COO64_RS03730 0.58 +0.7 225,414 + COO64_RS03730 0.58 +0.1 225,414 + COO64_RS03730 0.58 -0.1 225,414 + COO64_RS03730 0.58 -0.1 225,415 - COO64_RS03730 0.59 +0.7 225,415 - COO64_RS03730 0.59 +0.3 225,415 - COO64_RS03730 0.59 -0.6 225,415 - COO64_RS03730 0.59 -1.5 225,415 - COO64_RS03730 0.59 -0.2 225,415 - COO64_RS03730 0.59 +0.3 225,415 - COO64_RS03730 0.59 -1.1 225,586 + -0.3 225,587 - -2.0 225,587 - -0.1 225,587 - +0.6 225,587 - -0.2 225,636 + +1.2 225,636 + -1.8 225,636 + -0.7 225,636 + +0.6 225,636 + -0.2 225,636 + +0.2 225,637 - +1.8 225,637 - +0.2 225,637 - -0.3 225,637 - -0.1 225,637 - +0.6 225,637 - +0.1 225,819 + COO64_RS03735 0.34 -0.8 225,819 + COO64_RS03735 0.34 +0.4 225,819 + COO64_RS03735 0.34 -0.6 225,820 - COO64_RS03735 0.34 -1.3 225,820 - COO64_RS03735 0.34 +0.2 225,999 + COO64_RS03735 0.66 -1.2 225,999 + COO64_RS03735 0.66 -0.7 226,000 - COO64_RS03735 0.66 -0.0 226,153 + -1.0 226,153 + +1.4 226,153 + -1.4 226,153 + +0.9 226,154 - -0.8 226,154 - +1.0 226,155 + -0.6 226,156 - +0.1 226,156 - +0.6 226,156 - +0.2 226,195 + -0.3 226,195 + +0.9 226,196 - -0.0 226,196 - +0.4 226,223 + -0.5 226,223 + -0.0 226,224 - -0.8 226,224 - -0.2 226,258 + -0.3 226,259 - +0.2 226,275 + -0.2 226,275 + -1.0 226,275 + +0.3 226,276 - -0.2 226,299 + +0.1 226,461 + COO64_RS03740 0.38 +0.7 226,462 - COO64_RS03740 0.38 +0.9 226,462 - COO64_RS03740 0.38 -0.2 226,462 - COO64_RS03740 0.38 +0.2 226,572 + COO64_RS03740 0.59 +0.6 226,572 + COO64_RS03740 0.59 +0.2 226,572 + COO64_RS03740 0.59 -0.3 226,572 + COO64_RS03740 0.59 +0.2 226,572 + COO64_RS03740 0.59 +0.7 226,572 + COO64_RS03740 0.59 -0.7 226,572 + COO64_RS03740 0.59 +1.9 226,572 + COO64_RS03740 0.59 -0.1 226,572 + COO64_RS03740 0.59 +0.9 226,572 + COO64_RS03740 0.59 +0.0 226,572 + COO64_RS03740 0.59 -0.0 226,572 + COO64_RS03740 0.59 -0.4 226,572 + COO64_RS03740 0.59 -0.6 226,573 + COO64_RS03740 0.59 -0.2 226,573 - COO64_RS03740 0.59 -0.8 226,573 - COO64_RS03740 0.59 +0.6 226,573 - COO64_RS03740 0.59 +0.4 226,573 - COO64_RS03740 0.59 +1.4 226,573 - COO64_RS03740 0.59 -0.7 226,573 - COO64_RS03740 0.59 -1.6 226,573 - COO64_RS03740 0.59 +0.2 226,573 - COO64_RS03740 0.59 +0.0 226,573 - COO64_RS03740 0.59 -0.4 226,573 - COO64_RS03740 0.59 +0.8 226,573 - COO64_RS03740 0.59 -0.0 226,573 - COO64_RS03740 0.59 -1.0 226,573 - COO64_RS03740 0.59 +0.7 226,573 - COO64_RS03740 0.59 +0.1 226,640 + COO64_RS03740 0.71 +0.3 226,641 - COO64_RS03740 0.72 -0.3 226,696 + COO64_RS03740 0.82 -0.1 226,697 - COO64_RS03740 0.82 -0.1 226,697 - COO64_RS03740 0.82 -1.6 226,697 - COO64_RS03740 0.82 -0.1 226,730 + COO64_RS03740 0.88 +2.3 226,731 - COO64_RS03740 0.89 -1.6 226,731 - COO64_RS03740 0.89 -1.0 226,731 - COO64_RS03740 0.89 +0.5 226,763 - +0.5 226,763 - +0.6 226,802 + +1.0 226,803 - +0.4 226,826 + -0.2 226,826 + -0.4 226,826 + -0.7 226,856 + COO64_RS03745 0.12 +1.4 226,856 + COO64_RS03745 0.12 -0.6 226,856 + COO64_RS03745 0.12 -0.2 226,857 - COO64_RS03745 0.12 +0.0 226,857 - COO64_RS03745 0.12 +0.0 226,857 - COO64_RS03745 0.12 -0.4 226,857 - COO64_RS03745 0.12 +0.1 226,857 - COO64_RS03745 0.12 +0.2 226,857 - COO64_RS03745 0.12 -0.2 226,857 - COO64_RS03745 0.12 +0.7 226,932 - COO64_RS03745 0.26 -1.5 226,932 - COO64_RS03745 0.26 -0.1 226,932 - COO64_RS03745 0.26 -0.3 226,932 - COO64_RS03745 0.26 -1.3 227,024 + COO64_RS03745 0.42 +0.2 227,024 + COO64_RS03745 0.42 -0.1 227,025 - COO64_RS03745 0.43 -0.2 227,025 - COO64_RS03745 0.43 -2.4 227,076 - COO64_RS03745 0.52 +0.2 227,114 + COO64_RS03745 0.59 -0.5 227,114 + COO64_RS03745 0.59 -1.0 227,114 + COO64_RS03745 0.59 -0.7 227,114 + COO64_RS03745 0.59 +0.9 227,114 + COO64_RS03745 0.59 -0.5 227,114 + COO64_RS03745 0.59 +0.7 227,114 + COO64_RS03745 0.59 +0.2 227,114 + COO64_RS03745 0.59 -1.2 227,115 - COO64_RS03745 0.59 -0.0 227,115 - COO64_RS03745 0.59 +0.7 227,115 - COO64_RS03745 0.59 -0.3 227,115 - COO64_RS03745 0.59 +0.9 227,115 - COO64_RS03745 0.59 -0.2
Or see this region's nucleotide sequence