Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trxB and nadC are separated by 59 nucleotides nadC and COO64_RS03525 are separated by 254 nucleotides
COO64_RS03515: trxB - thioredoxin-disulfide reductase, at 181,423 to 182,385
trxB
COO64_RS03520: nadC - carboxylating nicotinate-nucleotide diphosphorylase, at 182,445 to 183,293
nadC
COO64_RS03525: COO64_RS03525 - DUF1631 domain-containing protein, at 183,548 to 185,746
_RS03525
Position (kb)
182
183
184 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 182.387 kb on - strand at 182.387 kb on - strand at 182.387 kb on - strand at 182.413 kb on + strand at 182.414 kb on - strand at 182.414 kb on - strand at 182.428 kb on + strand at 182.429 kb on - strand at 182.429 kb on - strand at 182.454 kb on - strand at 182.481 kb on + strand at 182.481 kb on + strand at 182.481 kb on + strand at 182.481 kb on + strand at 182.482 kb on - strand at 182.482 kb on - strand at 182.482 kb on - strand at 182.482 kb on - strand at 182.482 kb on - strand at 182.482 kb on - strand at 182.482 kb on - strand at 182.512 kb on + strand at 182.512 kb on + strand at 182.512 kb on + strand at 182.512 kb on + strand at 182.512 kb on + strand at 182.512 kb on + strand at 182.513 kb on - strand at 182.513 kb on - strand at 182.513 kb on - strand at 182.513 kb on - strand at 182.695 kb on - strand, within nadC at 182.695 kb on - strand, within nadC at 182.695 kb on - strand, within nadC at 182.803 kb on + strand, within nadC at 182.849 kb on - strand, within nadC at 183.310 kb on + strand at 183.310 kb on + strand at 183.310 kb on + strand at 183.310 kb on + strand at 183.310 kb on + strand at 183.310 kb on + strand at 183.311 kb on - strand at 183.311 kb on - strand at 183.311 kb on - strand at 183.311 kb on - strand at 183.325 kb on - strand at 183.325 kb on - strand at 183.325 kb on - strand at 183.326 kb on + strand at 183.326 kb on + strand at 183.326 kb on + strand at 183.326 kb on + strand at 183.326 kb on + strand at 183.327 kb on - strand at 183.327 kb on - strand at 183.327 kb on - strand at 183.407 kb on + strand at 183.423 kb on + strand at 183.484 kb on + strand at 183.496 kb on + strand at 183.497 kb on - strand at 183.706 kb on + strand at 183.706 kb on + strand at 183.707 kb on - strand at 183.707 kb on - strand at 183.827 kb on + strand, within COO64_RS03525 at 183.827 kb on + strand, within COO64_RS03525 at 183.827 kb on + strand, within COO64_RS03525 at 183.827 kb on + strand, within COO64_RS03525 at 183.827 kb on + strand, within COO64_RS03525 at 183.827 kb on + strand, within COO64_RS03525 at 183.827 kb on + strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 183.828 kb on - strand, within COO64_RS03525 at 184.074 kb on - strand, within COO64_RS03525 at 184.148 kb on + strand, within COO64_RS03525 at 184.148 kb on + strand, within COO64_RS03525 at 184.148 kb on + strand, within COO64_RS03525 at 184.148 kb on + strand, within COO64_RS03525 at 184.148 kb on + strand, within COO64_RS03525 at 184.149 kb on - strand, within COO64_RS03525 at 184.149 kb on - strand, within COO64_RS03525 at 184.149 kb on - strand, within COO64_RS03525 at 184.149 kb on - strand, within COO64_RS03525 at 184.149 kb on - strand, within COO64_RS03525 at 184.149 kb on - strand, within COO64_RS03525 at 184.175 kb on + strand, within COO64_RS03525 at 184.175 kb on + strand, within COO64_RS03525 at 184.175 kb on + strand, within COO64_RS03525 at 184.175 kb on + strand, within COO64_RS03525
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 182,387 - +0.1 182,387 - +0.6 182,387 - -0.1 182,413 + -0.6 182,414 - +0.3 182,414 - +0.8 182,428 + -0.4 182,429 - +0.8 182,429 - +0.0 182,454 - -0.8 182,481 + -0.2 182,481 + +0.6 182,481 + +0.4 182,481 + -0.2 182,482 - +1.3 182,482 - -0.1 182,482 - -0.1 182,482 - +2.4 182,482 - -0.1 182,482 - -0.6 182,482 - +0.5 182,512 + -0.7 182,512 + -0.7 182,512 + +0.2 182,512 + -1.1 182,512 + +1.7 182,512 + +0.2 182,513 - -0.1 182,513 - -0.1 182,513 - -0.5 182,513 - +1.4 182,695 - nadC COO64_RS03520 0.29 -0.9 182,695 - nadC COO64_RS03520 0.29 -0.2 182,695 - nadC COO64_RS03520 0.29 +0.7 182,803 + nadC COO64_RS03520 0.42 -1.6 182,849 - nadC COO64_RS03520 0.48 -1.6 183,310 + +0.0 183,310 + -1.0 183,310 + -0.8 183,310 + -3.1 183,310 + -0.1 183,310 + +0.6 183,311 - +0.1 183,311 - +1.0 183,311 - -0.8 183,311 - -0.4 183,325 - -1.3 183,325 - +0.0 183,325 - -0.4 183,326 + +0.1 183,326 + -0.5 183,326 + +0.3 183,326 + -2.2 183,326 + -0.4 183,327 - +0.3 183,327 - +0.7 183,327 - +0.2 183,407 + -1.3 183,423 + -0.1 183,484 + -0.4 183,496 + -0.1 183,497 - +0.7 183,706 + -0.4 183,706 + -0.1 183,707 - -0.2 183,707 - +0.5 183,827 + COO64_RS03525 0.13 -0.2 183,827 + COO64_RS03525 0.13 +0.0 183,827 + COO64_RS03525 0.13 -1.0 183,827 + COO64_RS03525 0.13 -0.6 183,827 + COO64_RS03525 0.13 +1.5 183,827 + COO64_RS03525 0.13 -0.3 183,827 + COO64_RS03525 0.13 -0.2 183,828 - COO64_RS03525 0.13 -0.4 183,828 - COO64_RS03525 0.13 -1.0 183,828 - COO64_RS03525 0.13 -2.1 183,828 - COO64_RS03525 0.13 +0.5 183,828 - COO64_RS03525 0.13 -0.6 183,828 - COO64_RS03525 0.13 +0.7 183,828 - COO64_RS03525 0.13 -0.3 183,828 - COO64_RS03525 0.13 +0.7 183,828 - COO64_RS03525 0.13 -0.0 184,074 - COO64_RS03525 0.24 -0.3 184,148 + COO64_RS03525 0.27 +1.9 184,148 + COO64_RS03525 0.27 -1.6 184,148 + COO64_RS03525 0.27 +0.1 184,148 + COO64_RS03525 0.27 +0.4 184,148 + COO64_RS03525 0.27 +0.2 184,149 - COO64_RS03525 0.27 +0.1 184,149 - COO64_RS03525 0.27 +1.4 184,149 - COO64_RS03525 0.27 -1.0 184,149 - COO64_RS03525 0.27 +0.3 184,149 - COO64_RS03525 0.27 -1.2 184,149 - COO64_RS03525 0.27 -0.7 184,175 + COO64_RS03525 0.29 -1.1 184,175 + COO64_RS03525 0.29 -0.1 184,175 + COO64_RS03525 0.29 -0.6 184,175 + COO64_RS03525 0.29 -1.0
Or see this region's nucleotide sequence