Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS03175 and ppk2 are separated by 34 nucleotides ppk2 and ychF are separated by 148 nucleotides ychF and pth are separated by 21 nucleotides pth and COO64_RS03195 are separated by 77 nucleotides
COO64_RS03175: COO64_RS03175 - MFS transporter, at 113,549 to 114,718
_RS03175
COO64_RS03180: ppk2 - polyphosphate kinase 2, at 114,753 to 115,562
ppk2
COO64_RS03185: ychF - redox-regulated ATPase YchF, at 115,711 to 116,811
ychF
COO64_RS03190: pth - aminoacyl-tRNA hydrolase, at 116,833 to 117,417
pth
COO64_RS03195: COO64_RS03195 - 50S ribosomal protein L25/general stress protein Ctc, at 117,495 to 118,091
_RS03195
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 114.716 kb on + strand at 114.716 kb on + strand at 114.717 kb on - strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.784 kb on + strand at 114.785 kb on - strand at 114.785 kb on - strand at 114.785 kb on - strand at 114.891 kb on - strand, within ppk2 at 114.891 kb on - strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.931 kb on + strand, within ppk2 at 114.932 kb on - strand, within ppk2 at 114.932 kb on - strand, within ppk2 at 114.932 kb on - strand, within ppk2 at 114.988 kb on + strand, within ppk2 at 114.988 kb on + strand, within ppk2 at 114.988 kb on + strand, within ppk2 at 114.988 kb on + strand, within ppk2 at 114.988 kb on + strand, within ppk2 at 114.989 kb on - strand, within ppk2 at 114.989 kb on - strand, within ppk2 at 114.989 kb on - strand, within ppk2 at 115.108 kb on + strand, within ppk2 at 115.109 kb on - strand, within ppk2 at 115.231 kb on + strand, within ppk2 at 115.231 kb on + strand, within ppk2 at 115.231 kb on + strand, within ppk2 at 115.231 kb on + strand, within ppk2 at 115.231 kb on + strand, within ppk2 at 115.232 kb on - strand, within ppk2 at 115.232 kb on - strand, within ppk2 at 115.232 kb on - strand, within ppk2 at 115.232 kb on - strand, within ppk2 at 115.232 kb on - strand, within ppk2 at 115.285 kb on + strand, within ppk2 at 115.285 kb on + strand, within ppk2 at 115.285 kb on + strand, within ppk2 at 115.285 kb on + strand, within ppk2 at 115.297 kb on + strand, within ppk2 at 115.297 kb on + strand, within ppk2 at 115.297 kb on + strand, within ppk2 at 115.297 kb on + strand, within ppk2 at 115.298 kb on - strand, within ppk2 at 115.298 kb on - strand, within ppk2 at 115.298 kb on - strand, within ppk2 at 115.298 kb on - strand, within ppk2 at 115.449 kb on + strand, within ppk2 at 115.507 kb on + strand at 115.507 kb on + strand at 115.508 kb on - strand at 115.508 kb on - strand at 115.557 kb on - strand at 115.613 kb on - strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.614 kb on + strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.615 kb on - strand at 115.675 kb on + strand at 115.675 kb on + strand at 115.757 kb on + strand at 115.757 kb on + strand at 115.757 kb on + strand at 115.757 kb on + strand at 115.757 kb on + strand at 115.757 kb on + strand at 115.758 kb on - strand at 115.758 kb on - strand at 115.758 kb on - strand at 115.758 kb on - strand at 115.758 kb on - strand at 115.758 kb on - strand at 115.758 kb on - strand at 115.783 kb on - strand at 115.814 kb on + strand at 115.814 kb on + strand at 115.814 kb on + strand at 115.814 kb on + strand at 115.815 kb on - strand at 115.815 kb on - strand at 115.833 kb on - strand, within ychF at 115.919 kb on - strand, within ychF at 115.933 kb on + strand, within ychF at 115.934 kb on - strand, within ychF at 115.958 kb on + strand, within ychF at 115.958 kb on + strand, within ychF at 115.958 kb on + strand, within ychF at 115.959 kb on - strand, within ychF at 115.959 kb on - strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.195 kb on + strand, within ychF at 116.196 kb on - strand, within ychF at 116.196 kb on - strand, within ychF at 116.196 kb on - strand, within ychF at 117.448 kb on - strand at 117.465 kb on - strand at 117.492 kb on - strand at 117.497 kb on - strand at 117.775 kb on - strand, within COO64_RS03195
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 114,716 + +0.5 114,716 + -0.3 114,717 - -0.2 114,784 + +1.6 114,784 + -0.7 114,784 + +0.0 114,784 + +0.2 114,784 + -0.2 114,784 + +0.2 114,784 + +0.1 114,784 + +0.2 114,784 + +0.6 114,785 - -0.4 114,785 - -0.2 114,785 - +0.2 114,891 - ppk2 COO64_RS03180 0.17 -0.4 114,891 - ppk2 COO64_RS03180 0.17 -0.9 114,931 + ppk2 COO64_RS03180 0.22 +0.8 114,931 + ppk2 COO64_RS03180 0.22 -0.6 114,931 + ppk2 COO64_RS03180 0.22 -0.6 114,931 + ppk2 COO64_RS03180 0.22 +1.3 114,931 + ppk2 COO64_RS03180 0.22 -0.5 114,931 + ppk2 COO64_RS03180 0.22 +0.2 114,931 + ppk2 COO64_RS03180 0.22 -0.2 114,932 - ppk2 COO64_RS03180 0.22 -0.3 114,932 - ppk2 COO64_RS03180 0.22 -0.1 114,932 - ppk2 COO64_RS03180 0.22 -1.6 114,988 + ppk2 COO64_RS03180 0.29 -0.2 114,988 + ppk2 COO64_RS03180 0.29 -0.5 114,988 + ppk2 COO64_RS03180 0.29 +0.7 114,988 + ppk2 COO64_RS03180 0.29 +0.6 114,988 + ppk2 COO64_RS03180 0.29 +0.0 114,989 - ppk2 COO64_RS03180 0.29 -1.2 114,989 - ppk2 COO64_RS03180 0.29 -0.3 114,989 - ppk2 COO64_RS03180 0.29 -2.4 115,108 + ppk2 COO64_RS03180 0.44 +0.4 115,109 - ppk2 COO64_RS03180 0.44 -0.6 115,231 + ppk2 COO64_RS03180 0.59 +0.6 115,231 + ppk2 COO64_RS03180 0.59 -0.9 115,231 + ppk2 COO64_RS03180 0.59 -0.5 115,231 + ppk2 COO64_RS03180 0.59 +0.3 115,231 + ppk2 COO64_RS03180 0.59 -0.2 115,232 - ppk2 COO64_RS03180 0.59 -0.9 115,232 - ppk2 COO64_RS03180 0.59 -0.8 115,232 - ppk2 COO64_RS03180 0.59 -1.0 115,232 - ppk2 COO64_RS03180 0.59 -2.2 115,232 - ppk2 COO64_RS03180 0.59 +0.0 115,285 + ppk2 COO64_RS03180 0.66 -0.7 115,285 + ppk2 COO64_RS03180 0.66 +0.4 115,285 + ppk2 COO64_RS03180 0.66 +0.9 115,285 + ppk2 COO64_RS03180 0.66 -0.1 115,297 + ppk2 COO64_RS03180 0.67 +1.5 115,297 + ppk2 COO64_RS03180 0.67 +0.1 115,297 + ppk2 COO64_RS03180 0.67 -1.7 115,297 + ppk2 COO64_RS03180 0.67 +0.1 115,298 - ppk2 COO64_RS03180 0.67 +0.6 115,298 - ppk2 COO64_RS03180 0.67 +0.2 115,298 - ppk2 COO64_RS03180 0.67 +0.2 115,298 - ppk2 COO64_RS03180 0.67 +0.0 115,449 + ppk2 COO64_RS03180 0.86 +0.5 115,507 + +0.2 115,507 + -0.1 115,508 - -0.3 115,508 - +0.2 115,557 - +0.4 115,613 - -0.2 115,614 + -0.5 115,614 + -1.0 115,614 + -0.4 115,614 + -0.1 115,614 + +0.7 115,614 + +0.1 115,614 + -1.2 115,614 + +0.3 115,614 + -0.4 115,614 + +0.5 115,615 - -1.4 115,615 - -0.1 115,615 - -0.4 115,615 - -1.1 115,615 - -0.2 115,615 - +1.8 115,615 - +0.9 115,615 - -1.3 115,615 - -0.0 115,615 - -0.3 115,675 + +0.5 115,675 + -0.2 115,757 + -0.7 115,757 + +0.1 115,757 + -0.6 115,757 + +0.7 115,757 + -1.0 115,757 + -0.8 115,758 - +0.4 115,758 - +0.4 115,758 - -0.2 115,758 - +0.3 115,758 - -2.1 115,758 - -2.4 115,758 - -1.7 115,783 - +0.6 115,814 + -0.6 115,814 + +1.0 115,814 + +0.7 115,814 + +0.6 115,815 - +1.4 115,815 - -1.1 115,833 - ychF COO64_RS03185 0.11 -0.2 115,919 - ychF COO64_RS03185 0.19 -1.2 115,933 + ychF COO64_RS03185 0.20 +0.1 115,934 - ychF COO64_RS03185 0.20 -0.6 115,958 + ychF COO64_RS03185 0.22 -0.7 115,958 + ychF COO64_RS03185 0.22 +0.0 115,958 + ychF COO64_RS03185 0.22 +0.0 115,959 - ychF COO64_RS03185 0.23 -0.2 115,959 - ychF COO64_RS03185 0.23 +1.2 116,195 + ychF COO64_RS03185 0.44 -1.3 116,195 + ychF COO64_RS03185 0.44 -1.2 116,195 + ychF COO64_RS03185 0.44 +0.2 116,195 + ychF COO64_RS03185 0.44 +0.0 116,195 + ychF COO64_RS03185 0.44 +1.0 116,195 + ychF COO64_RS03185 0.44 +0.1 116,195 + ychF COO64_RS03185 0.44 -0.9 116,195 + ychF COO64_RS03185 0.44 -0.2 116,196 - ychF COO64_RS03185 0.44 +1.0 116,196 - ychF COO64_RS03185 0.44 -1.4 116,196 - ychF COO64_RS03185 0.44 +1.0 117,448 - +0.1 117,465 - -0.6 117,492 - -0.8 117,497 - -1.7 117,775 - COO64_RS03195 0.47 +0.8
Or see this region's nucleotide sequence