Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recB and recD overlap by 4 nucleotides recD and COO64_RS02690 are separated by 113 nucleotides COO64_RS02690 and COO64_RS02695 overlap by 4 nucleotides
COO64_RS02680: recB - exodeoxyribonuclease V subunit beta, at 3,976 to 7,665
recB
COO64_RS02685: recD - exodeoxyribonuclease V subunit alpha, at 7,662 to 9,743
recD
COO64_RS02690: COO64_RS02690 - YfiR family protein, at 9,857 to 10,432
_RS02690
COO64_RS02695: COO64_RS02695 - diguanylate cyclase domain-containing protein, at 10,429 to 11,700
_RS02695
Position (kb)
7
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6.688 kb on + strand, within recB at 6.688 kb on + strand, within recB at 7.330 kb on + strand at 7.331 kb on - strand at 7.379 kb on - strand at 7.432 kb on + strand at 7.442 kb on - strand at 7.492 kb on + strand at 7.561 kb on + strand at 7.562 kb on - strand at 8.124 kb on + strand, within recD at 8.124 kb on + strand, within recD at 8.145 kb on + strand, within recD at 8.782 kb on - strand, within recD at 8.782 kb on - strand, within recD at 8.783 kb on + strand, within recD at 8.783 kb on + strand, within recD at 8.783 kb on + strand, within recD at 8.901 kb on + strand, within recD at 8.902 kb on - strand, within recD at 9.112 kb on - strand, within recD at 9.112 kb on - strand, within recD at 9.112 kb on - strand, within recD at 9.325 kb on - strand, within recD at 9.361 kb on - strand, within recD at 9.361 kb on - strand, within recD at 9.567 kb on - strand at 9.621 kb on + strand at 9.739 kb on + strand at 9.739 kb on + strand at 9.739 kb on + strand at 9.739 kb on + strand at 9.739 kb on + strand at 9.739 kb on + strand at 9.740 kb on - strand at 9.740 kb on - strand at 9.740 kb on - strand at 9.740 kb on - strand at 9.740 kb on - strand at 9.740 kb on - strand at 9.741 kb on + strand at 9.741 kb on + strand at 9.741 kb on + strand at 9.741 kb on + strand at 9.742 kb on - strand at 9.742 kb on - strand at 9.842 kb on + strand at 9.842 kb on + strand at 9.843 kb on - strand at 9.843 kb on - strand at 10.028 kb on + strand, within COO64_RS02690 at 10.028 kb on + strand, within COO64_RS02690 at 10.028 kb on + strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.029 kb on - strand, within COO64_RS02690 at 10.196 kb on + strand, within COO64_RS02690 at 10.196 kb on + strand, within COO64_RS02690 at 10.197 kb on - strand, within COO64_RS02690 at 10.197 kb on - strand, within COO64_RS02690 at 10.197 kb on - strand, within COO64_RS02690 at 10.199 kb on - strand, within COO64_RS02690 at 10.550 kb on + strand at 10.550 kb on + strand at 10.550 kb on + strand at 10.567 kb on + strand, within COO64_RS02695 at 10.567 kb on + strand, within COO64_RS02695 at 10.567 kb on + strand, within COO64_RS02695 at 10.567 kb on + strand, within COO64_RS02695 at 10.568 kb on - strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.609 kb on + strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.610 kb on - strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.724 kb on + strand, within COO64_RS02695 at 10.725 kb on - strand, within COO64_RS02695 at 10.725 kb on - strand, within COO64_RS02695 at 10.725 kb on - strand, within COO64_RS02695 at 10.725 kb on - strand, within COO64_RS02695
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 6,688 + recB COO64_RS02680 0.73 -1.6 6,688 + recB COO64_RS02680 0.73 -0.6 7,330 + +0.0 7,331 - +0.0 7,379 - -0.3 7,432 + -0.8 7,442 - -0.8 7,492 + -0.3 7,561 + -0.3 7,562 - -0.3 8,124 + recD COO64_RS02685 0.22 -1.3 8,124 + recD COO64_RS02685 0.22 -1.0 8,145 + recD COO64_RS02685 0.23 +0.4 8,782 - recD COO64_RS02685 0.54 -0.8 8,782 - recD COO64_RS02685 0.54 +0.1 8,783 + recD COO64_RS02685 0.54 -0.2 8,783 + recD COO64_RS02685 0.54 -0.3 8,783 + recD COO64_RS02685 0.54 -1.8 8,901 + recD COO64_RS02685 0.60 -1.8 8,902 - recD COO64_RS02685 0.60 +0.2 9,112 - recD COO64_RS02685 0.70 +0.4 9,112 - recD COO64_RS02685 0.70 -0.6 9,112 - recD COO64_RS02685 0.70 +0.0 9,325 - recD COO64_RS02685 0.80 +0.4 9,361 - recD COO64_RS02685 0.82 -1.2 9,361 - recD COO64_RS02685 0.82 -1.9 9,567 - -2.1 9,621 + +0.0 9,739 + +0.3 9,739 + +1.6 9,739 + +1.0 9,739 + +0.4 9,739 + +0.3 9,739 + -0.7 9,740 - +1.6 9,740 - -2.1 9,740 - -0.0 9,740 - -2.4 9,740 - +0.7 9,740 - -0.1 9,741 + +1.5 9,741 + +1.4 9,741 + +0.2 9,741 + +1.1 9,742 - +0.1 9,742 - +0.0 9,842 + -0.8 9,842 + +1.4 9,843 - -1.0 9,843 - -0.9 10,028 + COO64_RS02690 0.30 +0.4 10,028 + COO64_RS02690 0.30 +0.7 10,028 + COO64_RS02690 0.30 +1.0 10,029 - COO64_RS02690 0.30 -0.1 10,029 - COO64_RS02690 0.30 +0.3 10,029 - COO64_RS02690 0.30 -0.6 10,029 - COO64_RS02690 0.30 -0.9 10,029 - COO64_RS02690 0.30 +0.1 10,029 - COO64_RS02690 0.30 +0.1 10,029 - COO64_RS02690 0.30 +0.1 10,029 - COO64_RS02690 0.30 +0.7 10,029 - COO64_RS02690 0.30 +0.4 10,196 + COO64_RS02690 0.59 +0.0 10,196 + COO64_RS02690 0.59 +0.7 10,197 - COO64_RS02690 0.59 -2.1 10,197 - COO64_RS02690 0.59 -0.9 10,197 - COO64_RS02690 0.59 -0.4 10,199 - COO64_RS02690 0.59 -0.4 10,550 + -1.2 10,550 + -0.4 10,550 + +0.5 10,567 + COO64_RS02695 0.11 -0.0 10,567 + COO64_RS02695 0.11 -1.3 10,567 + COO64_RS02695 0.11 +0.4 10,567 + COO64_RS02695 0.11 -1.1 10,568 - COO64_RS02695 0.11 -0.8 10,609 + COO64_RS02695 0.14 -0.3 10,609 + COO64_RS02695 0.14 -1.3 10,609 + COO64_RS02695 0.14 -0.6 10,609 + COO64_RS02695 0.14 -0.9 10,609 + COO64_RS02695 0.14 -0.6 10,609 + COO64_RS02695 0.14 +3.0 10,609 + COO64_RS02695 0.14 -0.2 10,609 + COO64_RS02695 0.14 +0.1 10,609 + COO64_RS02695 0.14 -0.7 10,610 - COO64_RS02695 0.14 -0.2 10,610 - COO64_RS02695 0.14 -1.2 10,610 - COO64_RS02695 0.14 -0.7 10,610 - COO64_RS02695 0.14 +0.3 10,610 - COO64_RS02695 0.14 -0.4 10,610 - COO64_RS02695 0.14 -0.7 10,610 - COO64_RS02695 0.14 +0.5 10,610 - COO64_RS02695 0.14 +0.5 10,610 - COO64_RS02695 0.14 +0.8 10,610 - COO64_RS02695 0.14 -0.3 10,724 + COO64_RS02695 0.23 +1.2 10,724 + COO64_RS02695 0.23 -0.2 10,724 + COO64_RS02695 0.23 -0.2 10,724 + COO64_RS02695 0.23 +0.2 10,724 + COO64_RS02695 0.23 +0.1 10,724 + COO64_RS02695 0.23 -0.2 10,724 + COO64_RS02695 0.23 +1.3 10,724 + COO64_RS02695 0.23 +0.0 10,725 - COO64_RS02695 0.23 -0.1 10,725 - COO64_RS02695 0.23 -0.1 10,725 - COO64_RS02695 0.23 +0.5 10,725 - COO64_RS02695 0.23 -1.7
Or see this region's nucleotide sequence