Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS02485 and COO64_RS02490 are separated by 33 nucleotides COO64_RS02490 and dsbA are separated by 167 nucleotides dsbA and COO64_RS02500 are separated by 12 nucleotides COO64_RS02500 and COO64_RS02505 are separated by 27 nucleotides
COO64_RS02485: COO64_RS02485 - cytochrome c5 family protein, at 512,728 to 513,030
_RS02485
COO64_RS02490: COO64_RS02490 - cytochrome c, at 513,064 to 513,681
_RS02490
COO64_RS02495: dsbA - thiol:disulfide interchange protein DsbA, at 513,849 to 514,478
dsbA
COO64_RS02500: COO64_RS02500 - endonuclease/exonuclease/phosphatase family protein, at 514,491 to 515,345
_RS02500
COO64_RS02505: COO64_RS02505 - diguanylate cyclase, at 515,373 to 517,448
_RS02505
Position (kb) 513
514
515 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 512.901 kb on + strand, within COO64_RS02485 at 512.943 kb on + strand, within COO64_RS02485 at 512.943 kb on + strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.986 kb on + strand, within COO64_RS02485 at 512.987 kb on - strand, within COO64_RS02485 at 513.024 kb on + strand at 513.024 kb on + strand at 513.024 kb on + strand at 513.024 kb on + strand at 513.024 kb on + strand at 513.025 kb on - strand at 513.025 kb on - strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.049 kb on + strand at 513.049 kb on + strand at 513.049 kb on + strand at 513.050 kb on - strand at 513.050 kb on - strand at 513.050 kb on - strand at 513.050 kb on - strand at 513.058 kb on + strand at 513.058 kb on + strand at 513.059 kb on - strand at 513.075 kb on - strand at 513.075 kb on - strand at 513.132 kb on + strand, within COO64_RS02490 at 513.132 kb on + strand, within COO64_RS02490 at 513.133 kb on - strand, within COO64_RS02490 at 513.133 kb on - strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.250 kb on + strand, within COO64_RS02490 at 513.250 kb on + strand, within COO64_RS02490 at 513.251 kb on - strand, within COO64_RS02490 at 513.251 kb on - strand, within COO64_RS02490 at 513.348 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.582 kb on + strand, within COO64_RS02490 at 513.583 kb on - strand, within COO64_RS02490 at 513.583 kb on - strand, within COO64_RS02490 at 513.583 kb on - strand, within COO64_RS02490 at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.691 kb on + strand at 513.692 kb on - strand at 513.702 kb on + strand at 513.702 kb on + strand at 513.703 kb on - strand at 513.703 kb on - strand at 513.703 kb on - strand at 513.705 kb on + strand at 513.705 kb on + strand at 513.705 kb on + strand at 513.705 kb on + strand at 513.706 kb on - strand at 513.706 kb on - strand at 513.706 kb on - strand at 513.706 kb on - strand at 513.765 kb on + strand at 513.770 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.801 kb on - strand at 513.801 kb on - strand at 513.801 kb on - strand at 513.801 kb on - strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.829 kb on - strand at 513.829 kb on - strand at 513.829 kb on - strand at 513.829 kb on - strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.844 kb on - strand at 513.844 kb on - strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.855 kb on - strand at 513.939 kb on + strand, within dsbA at 513.939 kb on + strand, within dsbA at 513.939 kb on + strand, within dsbA at 513.940 kb on - strand, within dsbA at 513.940 kb on - strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.942 kb on - strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.028 kb on + strand, within dsbA at 514.028 kb on + strand, within dsbA at 514.028 kb on + strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.094 kb on - strand, within dsbA at 514.347 kb on + strand, within dsbA at 514.348 kb on - strand, within dsbA at 514.389 kb on + strand, within dsbA at 514.389 kb on + strand, within dsbA at 514.390 kb on - strand, within dsbA at 514.446 kb on + strand at 514.446 kb on + strand at 514.447 kb on - strand at 514.472 kb on + strand at 514.473 kb on - strand at 514.473 kb on - strand at 514.476 kb on + strand at 514.476 kb on + strand at 514.476 kb on + strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.644 kb on + strand, within COO64_RS02500 at 514.779 kb on + strand, within COO64_RS02500 at 514.779 kb on + strand, within COO64_RS02500 at 514.780 kb on - strand, within COO64_RS02500 at 514.824 kb on + strand, within COO64_RS02500 at 514.830 kb on + strand, within COO64_RS02500 at 514.831 kb on - strand, within COO64_RS02500 at 514.874 kb on + strand, within COO64_RS02500 at 514.875 kb on - strand, within COO64_RS02500 at 514.875 kb on - strand, within COO64_RS02500 at 515.043 kb on + strand, within COO64_RS02500 at 515.043 kb on + strand, within COO64_RS02500 at 515.043 kb on + strand, within COO64_RS02500 at 515.043 kb on + strand, within COO64_RS02500 at 515.043 kb on + strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.044 kb on - strand, within COO64_RS02500 at 515.134 kb on + strand, within COO64_RS02500 at 515.210 kb on + strand, within COO64_RS02500 at 515.210 kb on + strand, within COO64_RS02500 at 515.210 kb on + strand, within COO64_RS02500 at 515.211 kb on - strand, within COO64_RS02500 at 515.211 kb on - strand, within COO64_RS02500 at 515.265 kb on - strand at 515.265 kb on - strand at 515.324 kb on + strand at 515.324 kb on + strand at 515.325 kb on - strand at 515.325 kb on - strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.343 kb on + strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.344 kb on - strand at 515.354 kb on - strand at 515.407 kb on - strand at 515.407 kb on - strand at 515.417 kb on + strand at 515.417 kb on + strand at 515.417 kb on + strand at 515.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 512,901 + COO64_RS02485 0.57 +0.1 512,943 + COO64_RS02485 0.71 -0.3 512,943 + COO64_RS02485 0.71 +1.2 512,944 - COO64_RS02485 0.71 -1.4 512,944 - COO64_RS02485 0.71 +0.7 512,944 - COO64_RS02485 0.71 +0.5 512,944 - COO64_RS02485 0.71 -0.9 512,986 + COO64_RS02485 0.85 +0.1 512,987 - COO64_RS02485 0.85 +1.1 513,024 + -1.8 513,024 + -0.8 513,024 + -0.0 513,024 + -0.2 513,024 + -0.5 513,025 - +1.9 513,025 - -0.1 513,028 + +0.1 513,028 + -0.4 513,028 + +0.5 513,028 + -1.8 513,028 + +1.1 513,028 + -0.5 513,028 + -0.2 513,028 + -0.9 513,028 + -2.3 513,028 + -0.5 513,028 + -1.0 513,028 + -0.8 513,028 + -1.7 513,028 + +0.0 513,028 + +0.9 513,029 - +0.3 513,029 - -0.2 513,029 - -0.2 513,029 - -0.4 513,029 - +0.3 513,029 - -1.6 513,029 - -0.5 513,029 - +0.6 513,029 - -0.6 513,029 - +1.1 513,029 - +0.1 513,029 - +0.5 513,049 + -0.5 513,049 + +1.3 513,049 + -0.7 513,050 - -0.5 513,050 - -0.5 513,050 - -0.4 513,050 - +0.1 513,058 + -0.1 513,058 + +1.8 513,059 - +0.1 513,075 - -0.2 513,075 - -0.2 513,132 + COO64_RS02490 0.11 -1.5 513,132 + COO64_RS02490 0.11 -0.2 513,133 - COO64_RS02490 0.11 +2.1 513,133 - COO64_RS02490 0.11 -1.4 513,173 + COO64_RS02490 0.18 +0.7 513,173 + COO64_RS02490 0.18 +0.5 513,173 + COO64_RS02490 0.18 -0.9 513,173 + COO64_RS02490 0.18 +0.7 513,174 - COO64_RS02490 0.18 -0.7 513,174 - COO64_RS02490 0.18 -0.7 513,174 - COO64_RS02490 0.18 +0.1 513,174 - COO64_RS02490 0.18 +0.5 513,174 - COO64_RS02490 0.18 +1.8 513,174 - COO64_RS02490 0.18 -0.1 513,250 + COO64_RS02490 0.30 -0.7 513,250 + COO64_RS02490 0.30 +0.5 513,251 - COO64_RS02490 0.30 +0.5 513,251 - COO64_RS02490 0.30 -1.5 513,348 + COO64_RS02490 0.46 +0.1 513,358 + COO64_RS02490 0.48 -0.9 513,358 + COO64_RS02490 0.48 -0.5 513,358 + COO64_RS02490 0.48 -1.1 513,358 + COO64_RS02490 0.48 -1.7 513,358 + COO64_RS02490 0.48 -0.6 513,358 + COO64_RS02490 0.48 +0.5 513,358 + COO64_RS02490 0.48 -2.1 513,358 + COO64_RS02490 0.48 +0.5 513,358 + COO64_RS02490 0.48 +0.7 513,359 - COO64_RS02490 0.48 -1.2 513,359 - COO64_RS02490 0.48 -0.1 513,359 - COO64_RS02490 0.48 -0.1 513,359 - COO64_RS02490 0.48 +0.1 513,359 - COO64_RS02490 0.48 -0.6 513,359 - COO64_RS02490 0.48 +0.3 513,541 + COO64_RS02490 0.77 -1.2 513,541 + COO64_RS02490 0.77 -1.1 513,541 + COO64_RS02490 0.77 -1.5 513,541 + COO64_RS02490 0.77 +0.8 513,541 + COO64_RS02490 0.77 -0.2 513,541 + COO64_RS02490 0.77 +0.5 513,541 + COO64_RS02490 0.77 -0.2 513,542 - COO64_RS02490 0.77 -1.2 513,542 - COO64_RS02490 0.77 -1.1 513,542 - COO64_RS02490 0.77 +0.1 513,542 - COO64_RS02490 0.77 -0.4 513,542 - COO64_RS02490 0.77 +1.1 513,582 + COO64_RS02490 0.84 -0.5 513,583 - COO64_RS02490 0.84 -1.2 513,583 - COO64_RS02490 0.84 -2.0 513,583 - COO64_RS02490 0.84 -0.5 513,661 + -0.7 513,661 + -0.8 513,661 + -0.6 513,661 + +0.1 513,661 + -0.6 513,661 + -0.5 513,661 + +0.8 513,662 - -0.5 513,662 - +0.3 513,662 - -0.2 513,662 - -1.4 513,662 - +0.5 513,662 - -1.0 513,662 - -1.1 513,662 - +0.3 513,691 + +0.5 513,692 - -1.6 513,702 + -1.6 513,702 + -0.5 513,703 - -1.2 513,703 - -0.1 513,703 - -0.8 513,705 + -0.6 513,705 + -0.7 513,705 + +0.9 513,705 + +1.4 513,706 - -1.7 513,706 - -0.2 513,706 - +0.5 513,706 - -1.6 513,765 + +0.7 513,770 + +0.3 513,800 + +0.2 513,800 + -0.1 513,800 + +1.1 513,800 + +1.5 513,800 + -0.5 513,801 - +1.1 513,801 - -0.7 513,801 - -0.0 513,801 - -0.9 513,828 + +0.4 513,828 + -0.2 513,828 + +1.8 513,828 + -0.7 513,828 + -1.2 513,829 - +0.2 513,829 - +0.4 513,829 - +0.1 513,829 - +0.8 513,843 + +1.1 513,843 + -0.6 513,843 + -2.5 513,843 + -1.0 513,843 + +1.4 513,844 - +0.1 513,844 - +1.9 513,854 + -1.4 513,854 + -0.5 513,854 + -0.2 513,854 + +0.3 513,854 + -0.0 513,855 - +0.5 513,939 + dsbA COO64_RS02495 0.14 +0.5 513,939 + dsbA COO64_RS02495 0.14 -0.4 513,939 + dsbA COO64_RS02495 0.14 -0.6 513,940 - dsbA COO64_RS02495 0.14 -0.2 513,940 - dsbA COO64_RS02495 0.14 -0.9 513,941 + dsbA COO64_RS02495 0.15 +0.5 513,941 + dsbA COO64_RS02495 0.15 +0.1 513,941 + dsbA COO64_RS02495 0.15 -1.0 513,941 + dsbA COO64_RS02495 0.15 -0.3 513,942 - dsbA COO64_RS02495 0.15 -0.4 514,026 + dsbA COO64_RS02495 0.28 +1.1 514,026 + dsbA COO64_RS02495 0.28 +2.1 514,026 + dsbA COO64_RS02495 0.28 -0.2 514,026 + dsbA COO64_RS02495 0.28 +0.1 514,027 - dsbA COO64_RS02495 0.28 +0.5 514,027 - dsbA COO64_RS02495 0.28 -0.9 514,027 - dsbA COO64_RS02495 0.28 -0.5 514,027 - dsbA COO64_RS02495 0.28 -1.4 514,027 - dsbA COO64_RS02495 0.28 +0.6 514,027 - dsbA COO64_RS02495 0.28 -0.7 514,027 - dsbA COO64_RS02495 0.28 +1.3 514,027 - dsbA COO64_RS02495 0.28 -0.4 514,027 - dsbA COO64_RS02495 0.28 -0.9 514,028 + dsbA COO64_RS02495 0.28 -0.9 514,028 + dsbA COO64_RS02495 0.28 -0.4 514,028 + dsbA COO64_RS02495 0.28 -0.1 514,029 - dsbA COO64_RS02495 0.29 -0.2 514,029 - dsbA COO64_RS02495 0.29 -0.7 514,029 - dsbA COO64_RS02495 0.29 -0.2 514,029 - dsbA COO64_RS02495 0.29 +0.5 514,029 - dsbA COO64_RS02495 0.29 -2.4 514,029 - dsbA COO64_RS02495 0.29 -0.2 514,029 - dsbA COO64_RS02495 0.29 +0.1 514,029 - dsbA COO64_RS02495 0.29 -1.7 514,029 - dsbA COO64_RS02495 0.29 -0.9 514,029 - dsbA COO64_RS02495 0.29 +0.1 514,029 - dsbA COO64_RS02495 0.29 -1.2 514,094 - dsbA COO64_RS02495 0.39 -0.7 514,347 + dsbA COO64_RS02495 0.79 -0.4 514,348 - dsbA COO64_RS02495 0.79 -0.5 514,389 + dsbA COO64_RS02495 0.86 +0.5 514,389 + dsbA COO64_RS02495 0.86 +0.1 514,390 - dsbA COO64_RS02495 0.86 +1.3 514,446 + -1.4 514,446 + +0.4 514,447 - -1.6 514,472 + -2.8 514,473 - +0.4 514,473 - +0.4 514,476 + +0.6 514,476 + -0.2 514,476 + +0.0 514,477 - -0.0 514,477 - -0.4 514,477 - -0.3 514,477 - -0.1 514,477 - -0.5 514,477 - -0.3 514,644 + COO64_RS02500 0.18 +0.2 514,779 + COO64_RS02500 0.34 +0.1 514,779 + COO64_RS02500 0.34 -0.3 514,780 - COO64_RS02500 0.34 -0.0 514,824 + COO64_RS02500 0.39 -1.6 514,830 + COO64_RS02500 0.40 +0.4 514,831 - COO64_RS02500 0.40 -0.9 514,874 + COO64_RS02500 0.45 -1.3 514,875 - COO64_RS02500 0.45 +0.3 514,875 - COO64_RS02500 0.45 +0.2 515,043 + COO64_RS02500 0.65 -0.8 515,043 + COO64_RS02500 0.65 -1.9 515,043 + COO64_RS02500 0.65 -0.5 515,043 + COO64_RS02500 0.65 +0.2 515,043 + COO64_RS02500 0.65 +0.1 515,044 - COO64_RS02500 0.65 +0.0 515,044 - COO64_RS02500 0.65 -0.1 515,044 - COO64_RS02500 0.65 +0.2 515,044 - COO64_RS02500 0.65 -0.8 515,044 - COO64_RS02500 0.65 -0.6 515,044 - COO64_RS02500 0.65 -0.2 515,044 - COO64_RS02500 0.65 -0.3 515,044 - COO64_RS02500 0.65 +1.9 515,044 - COO64_RS02500 0.65 -0.2 515,134 + COO64_RS02500 0.75 +1.7 515,210 + COO64_RS02500 0.84 +1.7 515,210 + COO64_RS02500 0.84 +0.1 515,210 + COO64_RS02500 0.84 +0.3 515,211 - COO64_RS02500 0.84 -0.3 515,211 - COO64_RS02500 0.84 +0.3 515,265 - -0.4 515,265 - -0.9 515,324 + -1.2 515,324 + +0.2 515,325 - -1.8 515,325 - -0.2 515,343 + +0.9 515,343 + +0.3 515,343 + +0.6 515,343 + +0.0 515,343 + -0.2 515,343 + -0.2 515,344 - -0.1 515,344 - -0.2 515,344 - -0.2 515,344 - -0.0 515,344 - -0.4 515,344 - +0.6 515,344 - -0.6 515,344 - -0.6 515,344 - +1.0 515,344 - +0.8 515,354 - +0.0 515,407 - -0.9 515,407 - +0.1 515,417 + -0.1 515,417 + -0.1 515,417 + -0.9 515,417 + -0.5
Or see this region's nucleotide sequence