Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yihA and COO64_RS02485 are separated by 193 nucleotides COO64_RS02485 and COO64_RS02490 are separated by 33 nucleotides COO64_RS02490 and dsbA are separated by 167 nucleotides dsbA and COO64_RS02500 are separated by 12 nucleotides
COO64_RS02480: yihA - ribosome biogenesis GTP-binding protein YihA/YsxC, at 511,902 to 512,534
yihA
COO64_RS02485: COO64_RS02485 - cytochrome c5 family protein, at 512,728 to 513,030
_RS02485
COO64_RS02490: COO64_RS02490 - cytochrome c, at 513,064 to 513,681
_RS02490
COO64_RS02495: dsbA - thiol:disulfide interchange protein DsbA, at 513,849 to 514,478
dsbA
COO64_RS02500: COO64_RS02500 - endonuclease/exonuclease/phosphatase family protein, at 514,491 to 515,345
_RS02500
Position (kb) 513
514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 512.606 kb on + strand at 512.607 kb on - strand at 512.607 kb on - strand at 512.607 kb on - strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.608 kb on + strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.609 kb on - strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.610 kb on + strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.611 kb on - strand at 512.646 kb on + strand at 512.646 kb on + strand at 512.647 kb on - strand at 512.688 kb on + strand at 512.688 kb on + strand at 512.688 kb on + strand at 512.688 kb on + strand at 512.689 kb on - strand at 512.689 kb on - strand at 512.689 kb on - strand at 512.689 kb on - strand at 512.689 kb on - strand at 512.784 kb on + strand, within COO64_RS02485 at 512.806 kb on + strand, within COO64_RS02485 at 512.806 kb on + strand, within COO64_RS02485 at 512.807 kb on - strand, within COO64_RS02485 at 512.901 kb on + strand, within COO64_RS02485 at 512.943 kb on + strand, within COO64_RS02485 at 512.943 kb on + strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.944 kb on - strand, within COO64_RS02485 at 512.986 kb on + strand, within COO64_RS02485 at 512.987 kb on - strand, within COO64_RS02485 at 513.024 kb on + strand at 513.024 kb on + strand at 513.024 kb on + strand at 513.024 kb on + strand at 513.024 kb on + strand at 513.025 kb on - strand at 513.025 kb on - strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.028 kb on + strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.029 kb on - strand at 513.049 kb on + strand at 513.049 kb on + strand at 513.049 kb on + strand at 513.050 kb on - strand at 513.050 kb on - strand at 513.050 kb on - strand at 513.050 kb on - strand at 513.058 kb on + strand at 513.058 kb on + strand at 513.059 kb on - strand at 513.075 kb on - strand at 513.075 kb on - strand at 513.132 kb on + strand, within COO64_RS02490 at 513.132 kb on + strand, within COO64_RS02490 at 513.133 kb on - strand, within COO64_RS02490 at 513.133 kb on - strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.173 kb on + strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.174 kb on - strand, within COO64_RS02490 at 513.250 kb on + strand, within COO64_RS02490 at 513.250 kb on + strand, within COO64_RS02490 at 513.251 kb on - strand, within COO64_RS02490 at 513.251 kb on - strand, within COO64_RS02490 at 513.348 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.358 kb on + strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.359 kb on - strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.541 kb on + strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.542 kb on - strand, within COO64_RS02490 at 513.582 kb on + strand, within COO64_RS02490 at 513.583 kb on - strand, within COO64_RS02490 at 513.583 kb on - strand, within COO64_RS02490 at 513.583 kb on - strand, within COO64_RS02490 at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.661 kb on + strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.662 kb on - strand at 513.691 kb on + strand at 513.692 kb on - strand at 513.702 kb on + strand at 513.702 kb on + strand at 513.703 kb on - strand at 513.703 kb on - strand at 513.703 kb on - strand at 513.705 kb on + strand at 513.705 kb on + strand at 513.705 kb on + strand at 513.705 kb on + strand at 513.706 kb on - strand at 513.706 kb on - strand at 513.706 kb on - strand at 513.706 kb on - strand at 513.765 kb on + strand at 513.770 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.800 kb on + strand at 513.801 kb on - strand at 513.801 kb on - strand at 513.801 kb on - strand at 513.801 kb on - strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.828 kb on + strand at 513.829 kb on - strand at 513.829 kb on - strand at 513.829 kb on - strand at 513.829 kb on - strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.843 kb on + strand at 513.844 kb on - strand at 513.844 kb on - strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.854 kb on + strand at 513.855 kb on - strand at 513.939 kb on + strand, within dsbA at 513.939 kb on + strand, within dsbA at 513.939 kb on + strand, within dsbA at 513.940 kb on - strand, within dsbA at 513.940 kb on - strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.941 kb on + strand, within dsbA at 513.942 kb on - strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.026 kb on + strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.027 kb on - strand, within dsbA at 514.028 kb on + strand, within dsbA at 514.028 kb on + strand, within dsbA at 514.028 kb on + strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.029 kb on - strand, within dsbA at 514.094 kb on - strand, within dsbA at 514.347 kb on + strand, within dsbA at 514.348 kb on - strand, within dsbA at 514.389 kb on + strand, within dsbA at 514.389 kb on + strand, within dsbA at 514.390 kb on - strand, within dsbA at 514.446 kb on + strand at 514.446 kb on + strand at 514.447 kb on - strand at 514.472 kb on + strand at 514.473 kb on - strand at 514.473 kb on - strand at 514.476 kb on + strand at 514.476 kb on + strand at 514.476 kb on + strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.477 kb on - strand at 514.644 kb on + strand, within COO64_RS02500
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 512,606 + -2.0 512,607 - +0.5 512,607 - +0.2 512,607 - -0.5 512,608 + +0.3 512,608 + +0.5 512,608 + -0.9 512,608 + +1.2 512,608 + +0.4 512,608 + +0.7 512,608 + -1.6 512,608 + +0.3 512,608 + -0.2 512,608 + +1.7 512,608 + +1.6 512,608 + +0.9 512,608 + -1.8 512,608 + +0.3 512,609 - -1.5 512,609 - -0.7 512,609 - +0.5 512,609 - +1.5 512,609 - -1.6 512,609 - -2.2 512,609 - +0.5 512,609 - -1.2 512,609 - -0.4 512,610 + -0.3 512,610 + -0.1 512,610 + -0.1 512,610 + -0.4 512,610 + +1.4 512,610 + -0.2 512,610 + +0.0 512,610 + -1.9 512,610 + -0.7 512,610 + +0.0 512,610 + -1.9 512,611 - +1.3 512,611 - -1.1 512,611 - -0.5 512,611 - +0.6 512,611 - -0.9 512,611 - +0.3 512,611 - +0.6 512,611 - -0.5 512,611 - -0.0 512,611 - +0.9 512,611 - +0.1 512,611 - -0.6 512,646 + -0.3 512,646 + -0.0 512,647 - +0.2 512,688 + +0.1 512,688 + -0.3 512,688 + -1.2 512,688 + -0.1 512,689 - -0.7 512,689 - +0.3 512,689 - +0.8 512,689 - -0.1 512,689 - -1.6 512,784 + COO64_RS02485 0.18 +0.3 512,806 + COO64_RS02485 0.26 -0.3 512,806 + COO64_RS02485 0.26 -0.4 512,807 - COO64_RS02485 0.26 -1.6 512,901 + COO64_RS02485 0.57 +0.1 512,943 + COO64_RS02485 0.71 -0.3 512,943 + COO64_RS02485 0.71 +1.2 512,944 - COO64_RS02485 0.71 -1.4 512,944 - COO64_RS02485 0.71 +0.7 512,944 - COO64_RS02485 0.71 +0.5 512,944 - COO64_RS02485 0.71 -0.9 512,986 + COO64_RS02485 0.85 +0.1 512,987 - COO64_RS02485 0.85 +1.1 513,024 + -1.8 513,024 + -0.8 513,024 + -0.0 513,024 + -0.2 513,024 + -0.5 513,025 - +1.9 513,025 - -0.1 513,028 + +0.1 513,028 + -0.4 513,028 + +0.5 513,028 + -1.8 513,028 + +1.1 513,028 + -0.5 513,028 + -0.2 513,028 + -0.9 513,028 + -2.3 513,028 + -0.5 513,028 + -1.0 513,028 + -0.8 513,028 + -1.7 513,028 + +0.0 513,028 + +0.9 513,029 - +0.3 513,029 - -0.2 513,029 - -0.2 513,029 - -0.4 513,029 - +0.3 513,029 - -1.6 513,029 - -0.5 513,029 - +0.6 513,029 - -0.6 513,029 - +1.1 513,029 - +0.1 513,029 - +0.5 513,049 + -0.5 513,049 + +1.3 513,049 + -0.7 513,050 - -0.5 513,050 - -0.5 513,050 - -0.4 513,050 - +0.1 513,058 + -0.1 513,058 + +1.8 513,059 - +0.1 513,075 - -0.2 513,075 - -0.2 513,132 + COO64_RS02490 0.11 -1.5 513,132 + COO64_RS02490 0.11 -0.2 513,133 - COO64_RS02490 0.11 +2.1 513,133 - COO64_RS02490 0.11 -1.4 513,173 + COO64_RS02490 0.18 +0.7 513,173 + COO64_RS02490 0.18 +0.5 513,173 + COO64_RS02490 0.18 -0.9 513,173 + COO64_RS02490 0.18 +0.7 513,174 - COO64_RS02490 0.18 -0.7 513,174 - COO64_RS02490 0.18 -0.7 513,174 - COO64_RS02490 0.18 +0.1 513,174 - COO64_RS02490 0.18 +0.5 513,174 - COO64_RS02490 0.18 +1.8 513,174 - COO64_RS02490 0.18 -0.1 513,250 + COO64_RS02490 0.30 -0.7 513,250 + COO64_RS02490 0.30 +0.5 513,251 - COO64_RS02490 0.30 +0.5 513,251 - COO64_RS02490 0.30 -1.5 513,348 + COO64_RS02490 0.46 +0.1 513,358 + COO64_RS02490 0.48 -0.9 513,358 + COO64_RS02490 0.48 -0.5 513,358 + COO64_RS02490 0.48 -1.1 513,358 + COO64_RS02490 0.48 -1.7 513,358 + COO64_RS02490 0.48 -0.6 513,358 + COO64_RS02490 0.48 +0.5 513,358 + COO64_RS02490 0.48 -2.1 513,358 + COO64_RS02490 0.48 +0.5 513,358 + COO64_RS02490 0.48 +0.7 513,359 - COO64_RS02490 0.48 -1.2 513,359 - COO64_RS02490 0.48 -0.1 513,359 - COO64_RS02490 0.48 -0.1 513,359 - COO64_RS02490 0.48 +0.1 513,359 - COO64_RS02490 0.48 -0.6 513,359 - COO64_RS02490 0.48 +0.3 513,541 + COO64_RS02490 0.77 -1.2 513,541 + COO64_RS02490 0.77 -1.1 513,541 + COO64_RS02490 0.77 -1.5 513,541 + COO64_RS02490 0.77 +0.8 513,541 + COO64_RS02490 0.77 -0.2 513,541 + COO64_RS02490 0.77 +0.5 513,541 + COO64_RS02490 0.77 -0.2 513,542 - COO64_RS02490 0.77 -1.2 513,542 - COO64_RS02490 0.77 -1.1 513,542 - COO64_RS02490 0.77 +0.1 513,542 - COO64_RS02490 0.77 -0.4 513,542 - COO64_RS02490 0.77 +1.1 513,582 + COO64_RS02490 0.84 -0.5 513,583 - COO64_RS02490 0.84 -1.2 513,583 - COO64_RS02490 0.84 -2.0 513,583 - COO64_RS02490 0.84 -0.5 513,661 + -0.7 513,661 + -0.8 513,661 + -0.6 513,661 + +0.1 513,661 + -0.6 513,661 + -0.5 513,661 + +0.8 513,662 - -0.5 513,662 - +0.3 513,662 - -0.2 513,662 - -1.4 513,662 - +0.5 513,662 - -1.0 513,662 - -1.1 513,662 - +0.3 513,691 + +0.5 513,692 - -1.6 513,702 + -1.6 513,702 + -0.5 513,703 - -1.2 513,703 - -0.1 513,703 - -0.8 513,705 + -0.6 513,705 + -0.7 513,705 + +0.9 513,705 + +1.4 513,706 - -1.7 513,706 - -0.2 513,706 - +0.5 513,706 - -1.6 513,765 + +0.7 513,770 + +0.3 513,800 + +0.2 513,800 + -0.1 513,800 + +1.1 513,800 + +1.5 513,800 + -0.5 513,801 - +1.1 513,801 - -0.7 513,801 - -0.0 513,801 - -0.9 513,828 + +0.4 513,828 + -0.2 513,828 + +1.8 513,828 + -0.7 513,828 + -1.2 513,829 - +0.2 513,829 - +0.4 513,829 - +0.1 513,829 - +0.8 513,843 + +1.1 513,843 + -0.6 513,843 + -2.5 513,843 + -1.0 513,843 + +1.4 513,844 - +0.1 513,844 - +1.9 513,854 + -1.4 513,854 + -0.5 513,854 + -0.2 513,854 + +0.3 513,854 + -0.0 513,855 - +0.5 513,939 + dsbA COO64_RS02495 0.14 +0.5 513,939 + dsbA COO64_RS02495 0.14 -0.4 513,939 + dsbA COO64_RS02495 0.14 -0.6 513,940 - dsbA COO64_RS02495 0.14 -0.2 513,940 - dsbA COO64_RS02495 0.14 -0.9 513,941 + dsbA COO64_RS02495 0.15 +0.5 513,941 + dsbA COO64_RS02495 0.15 +0.1 513,941 + dsbA COO64_RS02495 0.15 -1.0 513,941 + dsbA COO64_RS02495 0.15 -0.3 513,942 - dsbA COO64_RS02495 0.15 -0.4 514,026 + dsbA COO64_RS02495 0.28 +1.1 514,026 + dsbA COO64_RS02495 0.28 +2.1 514,026 + dsbA COO64_RS02495 0.28 -0.2 514,026 + dsbA COO64_RS02495 0.28 +0.1 514,027 - dsbA COO64_RS02495 0.28 +0.5 514,027 - dsbA COO64_RS02495 0.28 -0.9 514,027 - dsbA COO64_RS02495 0.28 -0.5 514,027 - dsbA COO64_RS02495 0.28 -1.4 514,027 - dsbA COO64_RS02495 0.28 +0.6 514,027 - dsbA COO64_RS02495 0.28 -0.7 514,027 - dsbA COO64_RS02495 0.28 +1.3 514,027 - dsbA COO64_RS02495 0.28 -0.4 514,027 - dsbA COO64_RS02495 0.28 -0.9 514,028 + dsbA COO64_RS02495 0.28 -0.9 514,028 + dsbA COO64_RS02495 0.28 -0.4 514,028 + dsbA COO64_RS02495 0.28 -0.1 514,029 - dsbA COO64_RS02495 0.29 -0.2 514,029 - dsbA COO64_RS02495 0.29 -0.7 514,029 - dsbA COO64_RS02495 0.29 -0.2 514,029 - dsbA COO64_RS02495 0.29 +0.5 514,029 - dsbA COO64_RS02495 0.29 -2.4 514,029 - dsbA COO64_RS02495 0.29 -0.2 514,029 - dsbA COO64_RS02495 0.29 +0.1 514,029 - dsbA COO64_RS02495 0.29 -1.7 514,029 - dsbA COO64_RS02495 0.29 -0.9 514,029 - dsbA COO64_RS02495 0.29 +0.1 514,029 - dsbA COO64_RS02495 0.29 -1.2 514,094 - dsbA COO64_RS02495 0.39 -0.7 514,347 + dsbA COO64_RS02495 0.79 -0.4 514,348 - dsbA COO64_RS02495 0.79 -0.5 514,389 + dsbA COO64_RS02495 0.86 +0.5 514,389 + dsbA COO64_RS02495 0.86 +0.1 514,390 - dsbA COO64_RS02495 0.86 +1.3 514,446 + -1.4 514,446 + +0.4 514,447 - -1.6 514,472 + -2.8 514,473 - +0.4 514,473 - +0.4 514,476 + +0.6 514,476 + -0.2 514,476 + +0.0 514,477 - -0.0 514,477 - -0.4 514,477 - -0.3 514,477 - -0.1 514,477 - -0.5 514,477 - -0.3 514,644 + COO64_RS02500 0.18 +0.2
Or see this region's nucleotide sequence