Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS02415 and COO64_RS02420 are separated by 93 nucleotides COO64_RS02420 and COO64_RS02425 are separated by 62 nucleotides COO64_RS02425 and COO64_RS02430 overlap by 1 nucleotides
COO64_RS02415: COO64_RS02415 - SCO family protein, at 497,886 to 498,518
_RS02415
COO64_RS02420: COO64_RS02420 - MetQ/NlpA family ABC transporter substrate-binding protein, at 498,612 to 499,382
_RS02420
COO64_RS02425: COO64_RS02425 - methionine ABC transporter permease, at 499,445 to 500,116
_RS02425
COO64_RS02430: COO64_RS02430 - methionine ABC transporter ATP-binding protein, at 500,116 to 501,123
_RS02430
Position (kb) 499
500
501 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 498.640 kb on + strand at 498.640 kb on + strand at 498.640 kb on + strand at 498.640 kb on + strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.641 kb on - strand at 498.748 kb on + strand, within COO64_RS02420 at 498.748 kb on + strand, within COO64_RS02420 at 498.749 kb on - strand, within COO64_RS02420 at 498.749 kb on - strand, within COO64_RS02420 at 498.814 kb on + strand, within COO64_RS02420 at 498.814 kb on + strand, within COO64_RS02420 at 498.814 kb on + strand, within COO64_RS02420 at 498.815 kb on - strand, within COO64_RS02420 at 498.815 kb on - strand, within COO64_RS02420 at 498.815 kb on - strand, within COO64_RS02420 at 498.815 kb on - strand, within COO64_RS02420 at 498.815 kb on - strand, within COO64_RS02420 at 498.815 kb on - strand, within COO64_RS02420 at 499.060 kb on + strand, within COO64_RS02420 at 499.060 kb on + strand, within COO64_RS02420 at 499.060 kb on + strand, within COO64_RS02420 at 499.060 kb on + strand, within COO64_RS02420 at 499.060 kb on + strand, within COO64_RS02420 at 499.061 kb on - strand, within COO64_RS02420 at 499.061 kb on - strand, within COO64_RS02420 at 499.061 kb on - strand, within COO64_RS02420 at 499.061 kb on - strand, within COO64_RS02420 at 499.061 kb on - strand, within COO64_RS02420 at 499.072 kb on + strand, within COO64_RS02420 at 499.073 kb on - strand, within COO64_RS02420 at 499.150 kb on + strand, within COO64_RS02420 at 499.150 kb on + strand, within COO64_RS02420 at 499.214 kb on + strand, within COO64_RS02420 at 499.214 kb on + strand, within COO64_RS02420 at 499.215 kb on - strand, within COO64_RS02420 at 499.215 kb on - strand, within COO64_RS02420 at 499.215 kb on - strand, within COO64_RS02420 at 499.215 kb on - strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.216 kb on + strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.217 kb on - strand, within COO64_RS02420 at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.446 kb on + strand at 499.447 kb on - strand at 499.447 kb on - strand at 499.447 kb on - strand at 499.447 kb on - strand at 499.447 kb on - strand at 499.451 kb on - strand at 499.505 kb on + strand at 499.506 kb on - strand at 499.620 kb on + strand, within COO64_RS02425 at 499.620 kb on + strand, within COO64_RS02425 at 499.621 kb on - strand, within COO64_RS02425 at 499.621 kb on - strand, within COO64_RS02425 at 499.621 kb on - strand, within COO64_RS02425 at 499.621 kb on - strand, within COO64_RS02425 at 499.634 kb on - strand, within COO64_RS02425 at 500.059 kb on + strand at 500.060 kb on - strand at 500.060 kb on - strand at 500.062 kb on + strand at 500.062 kb on + strand at 500.063 kb on - strand at 500.125 kb on + strand at 500.125 kb on + strand at 500.125 kb on + strand at 500.125 kb on + strand at 500.125 kb on + strand at 500.126 kb on - strand at 500.133 kb on + strand at 500.133 kb on + strand at 500.133 kb on + strand at 500.134 kb on - strand at 500.134 kb on - strand at 500.134 kb on - strand at 500.134 kb on - strand at 500.134 kb on - strand at 500.250 kb on + strand, within COO64_RS02430 at 500.250 kb on + strand, within COO64_RS02430 at 500.251 kb on - strand, within COO64_RS02430 at 500.251 kb on - strand, within COO64_RS02430 at 500.255 kb on + strand, within COO64_RS02430 at 500.255 kb on + strand, within COO64_RS02430 at 500.255 kb on + strand, within COO64_RS02430 at 500.255 kb on + strand, within COO64_RS02430 at 500.255 kb on + strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.256 kb on - strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.300 kb on + strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.301 kb on - strand, within COO64_RS02430 at 500.995 kb on - strand, within COO64_RS02430 at 501.025 kb on + strand at 501.026 kb on - strand at 501.026 kb on - strand at 501.026 kb on - strand at 501.026 kb on - strand at 501.026 kb on - strand at 501.093 kb on - strand at 501.093 kb on - strand at 501.093 kb on - strand at 501.099 kb on + strand at 501.099 kb on + strand at 501.099 kb on + strand at 501.100 kb on - strand at 501.100 kb on - strand at 501.100 kb on - strand at 501.100 kb on - strand at 501.100 kb on - strand at 501.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 498,640 + -1.0 498,640 + +0.5 498,640 + -0.3 498,640 + -0.3 498,641 - -1.5 498,641 - -0.8 498,641 - +0.0 498,641 - -0.6 498,641 - -0.9 498,641 - -0.2 498,641 - +0.2 498,641 - -0.2 498,641 - -0.0 498,641 - -0.4 498,641 - +0.9 498,748 + COO64_RS02420 0.18 -0.1 498,748 + COO64_RS02420 0.18 -0.7 498,749 - COO64_RS02420 0.18 -0.4 498,749 - COO64_RS02420 0.18 -0.6 498,814 + COO64_RS02420 0.26 +1.1 498,814 + COO64_RS02420 0.26 -0.0 498,814 + COO64_RS02420 0.26 -0.0 498,815 - COO64_RS02420 0.26 +0.2 498,815 - COO64_RS02420 0.26 -0.5 498,815 - COO64_RS02420 0.26 +0.0 498,815 - COO64_RS02420 0.26 -0.8 498,815 - COO64_RS02420 0.26 -0.2 498,815 - COO64_RS02420 0.26 +1.0 499,060 + COO64_RS02420 0.58 -0.6 499,060 + COO64_RS02420 0.58 +0.3 499,060 + COO64_RS02420 0.58 -0.4 499,060 + COO64_RS02420 0.58 +1.1 499,060 + COO64_RS02420 0.58 -0.2 499,061 - COO64_RS02420 0.58 +0.4 499,061 - COO64_RS02420 0.58 -0.3 499,061 - COO64_RS02420 0.58 +0.5 499,061 - COO64_RS02420 0.58 +0.2 499,061 - COO64_RS02420 0.58 -1.9 499,072 + COO64_RS02420 0.60 -0.2 499,073 - COO64_RS02420 0.60 -0.0 499,150 + COO64_RS02420 0.70 -1.2 499,150 + COO64_RS02420 0.70 -0.5 499,214 + COO64_RS02420 0.78 -0.0 499,214 + COO64_RS02420 0.78 -0.1 499,215 - COO64_RS02420 0.78 -0.2 499,215 - COO64_RS02420 0.78 -1.8 499,215 - COO64_RS02420 0.78 +0.3 499,215 - COO64_RS02420 0.78 -0.5 499,216 + COO64_RS02420 0.78 +0.2 499,216 + COO64_RS02420 0.78 +0.3 499,216 + COO64_RS02420 0.78 -0.6 499,216 + COO64_RS02420 0.78 -1.1 499,216 + COO64_RS02420 0.78 -0.7 499,216 + COO64_RS02420 0.78 -1.1 499,216 + COO64_RS02420 0.78 -0.5 499,216 + COO64_RS02420 0.78 +0.8 499,216 + COO64_RS02420 0.78 -0.1 499,216 + COO64_RS02420 0.78 -1.1 499,216 + COO64_RS02420 0.78 +0.4 499,217 - COO64_RS02420 0.78 -0.6 499,217 - COO64_RS02420 0.78 +1.8 499,217 - COO64_RS02420 0.78 -0.4 499,217 - COO64_RS02420 0.78 -0.6 499,217 - COO64_RS02420 0.78 +0.1 499,217 - COO64_RS02420 0.78 -1.2 499,217 - COO64_RS02420 0.78 -1.0 499,217 - COO64_RS02420 0.78 -1.0 499,217 - COO64_RS02420 0.78 -1.5 499,217 - COO64_RS02420 0.78 +0.9 499,217 - COO64_RS02420 0.78 -0.4 499,446 + +0.2 499,446 + +1.1 499,446 + -0.1 499,446 + -0.5 499,446 + -0.7 499,446 + -0.2 499,446 + -0.1 499,446 + -1.9 499,446 + -0.5 499,447 - -1.2 499,447 - +0.3 499,447 - -0.5 499,447 - +0.7 499,447 - -0.5 499,451 - -0.0 499,505 + -2.2 499,506 - -0.1 499,620 + COO64_RS02425 0.26 -0.7 499,620 + COO64_RS02425 0.26 -1.1 499,621 - COO64_RS02425 0.26 +0.2 499,621 - COO64_RS02425 0.26 -1.1 499,621 - COO64_RS02425 0.26 -0.5 499,621 - COO64_RS02425 0.26 +0.1 499,634 - COO64_RS02425 0.28 +0.6 500,059 + -0.5 500,060 - -0.1 500,060 - -0.3 500,062 + -3.2 500,062 + +1.1 500,063 - -0.2 500,125 + +0.8 500,125 + +1.1 500,125 + -0.3 500,125 + -0.7 500,125 + +0.7 500,126 - +0.7 500,133 + -0.4 500,133 + +0.8 500,133 + +0.9 500,134 - +1.1 500,134 - -0.5 500,134 - +0.6 500,134 - +0.7 500,134 - -0.5 500,250 + COO64_RS02430 0.13 -0.9 500,250 + COO64_RS02430 0.13 -0.3 500,251 - COO64_RS02430 0.13 +0.6 500,251 - COO64_RS02430 0.13 +0.3 500,255 + COO64_RS02430 0.14 +0.5 500,255 + COO64_RS02430 0.14 +0.0 500,255 + COO64_RS02430 0.14 -0.7 500,255 + COO64_RS02430 0.14 -0.2 500,255 + COO64_RS02430 0.14 +0.8 500,256 - COO64_RS02430 0.14 -0.5 500,256 - COO64_RS02430 0.14 -0.4 500,256 - COO64_RS02430 0.14 -0.4 500,256 - COO64_RS02430 0.14 -0.3 500,256 - COO64_RS02430 0.14 +0.5 500,256 - COO64_RS02430 0.14 +1.2 500,256 - COO64_RS02430 0.14 +0.1 500,256 - COO64_RS02430 0.14 -0.1 500,256 - COO64_RS02430 0.14 -0.7 500,256 - COO64_RS02430 0.14 -0.2 500,300 + COO64_RS02430 0.18 +0.4 500,300 + COO64_RS02430 0.18 +0.3 500,300 + COO64_RS02430 0.18 -0.2 500,300 + COO64_RS02430 0.18 +0.7 500,300 + COO64_RS02430 0.18 -1.2 500,300 + COO64_RS02430 0.18 -0.4 500,300 + COO64_RS02430 0.18 -0.7 500,301 - COO64_RS02430 0.18 -1.5 500,301 - COO64_RS02430 0.18 +0.8 500,301 - COO64_RS02430 0.18 -0.6 500,301 - COO64_RS02430 0.18 +0.1 500,301 - COO64_RS02430 0.18 -0.2 500,301 - COO64_RS02430 0.18 +0.3 500,301 - COO64_RS02430 0.18 +0.8 500,995 - COO64_RS02430 0.87 -1.2 501,025 + -1.2 501,026 - -0.3 501,026 - -1.4 501,026 - +1.6 501,026 - -0.8 501,026 - +0.0 501,093 - -1.2 501,093 - -0.6 501,093 - -0.5 501,099 + -0.2 501,099 + +0.1 501,099 + -0.8 501,100 - +0.1 501,100 - +0.8 501,100 - -0.5 501,100 - +0.5 501,100 - +0.9 501,100 - -0.5
Or see this region's nucleotide sequence