Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyQ and COO64_RS02195 are separated by 82 nucleotides COO64_RS02195 and COO64_RS02200 are separated by 39 nucleotides COO64_RS02200 and COO64_RS02205 are separated by 92 nucleotides COO64_RS02205 and trkA are separated by 12 nucleotides
COO64_RS02190: glyQ - glycine--tRNA ligase subunit alpha, at 456,886 to 457,833
glyQ
COO64_RS02195: COO64_RS02195 - DNA-3-methyladenine glycosylase I, at 457,916 to 458,467
_RS02195
COO64_RS02200: COO64_RS02200 - lysophospholipid acyltransferase, at 458,507 to 459,394
_RS02200
COO64_RS02205: COO64_RS02205 - tetratricopeptide repeat protein, at 459,487 to 459,798
_RS02205
COO64_RS02210: trkA - Trk system potassium transporter TrkA, at 459,811 to 461,184
trkA
Position (kb) 458
459
460 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 457.880 kb on - strand at 457.902 kb on - strand at 457.915 kb on + strand at 457.915 kb on + strand at 457.915 kb on + strand at 457.961 kb on + strand at 457.961 kb on + strand at 457.961 kb on + strand at 457.962 kb on - strand at 457.962 kb on - strand at 458.091 kb on + strand, within COO64_RS02195 at 458.091 kb on + strand, within COO64_RS02195 at 458.091 kb on + strand, within COO64_RS02195 at 458.092 kb on - strand, within COO64_RS02195 at 458.299 kb on + strand, within COO64_RS02195 at 458.300 kb on - strand, within COO64_RS02195 at 458.472 kb on + strand at 458.472 kb on + strand at 458.472 kb on + strand at 458.473 kb on - strand at 458.858 kb on - strand, within COO64_RS02200 at 458.858 kb on - strand, within COO64_RS02200 at 458.897 kb on + strand, within COO64_RS02200 at 458.897 kb on + strand, within COO64_RS02200 at 458.897 kb on + strand, within COO64_RS02200 at 458.897 kb on + strand, within COO64_RS02200 at 458.897 kb on + strand, within COO64_RS02200 at 458.898 kb on - strand, within COO64_RS02200 at 458.898 kb on - strand, within COO64_RS02200 at 458.898 kb on - strand, within COO64_RS02200 at 458.898 kb on - strand, within COO64_RS02200 at 458.898 kb on - strand, within COO64_RS02200 at 458.898 kb on - strand, within COO64_RS02200 at 459.257 kb on + strand, within COO64_RS02200 at 459.257 kb on + strand, within COO64_RS02200 at 459.257 kb on + strand, within COO64_RS02200 at 459.257 kb on + strand, within COO64_RS02200 at 459.257 kb on + strand, within COO64_RS02200 at 459.311 kb on + strand at 459.311 kb on + strand at 459.311 kb on + strand at 459.311 kb on + strand at 459.311 kb on + strand at 459.311 kb on + strand at 459.311 kb on + strand at 459.311 kb on + strand at 459.312 kb on - strand at 459.312 kb on - strand at 459.312 kb on - strand at 459.312 kb on - strand at 459.312 kb on - strand at 459.312 kb on - strand at 459.335 kb on + strand at 459.335 kb on + strand at 459.336 kb on - strand at 459.336 kb on - strand at 459.336 kb on - strand at 459.336 kb on - strand at 459.390 kb on - strand at 459.390 kb on - strand at 459.390 kb on - strand at 459.489 kb on - strand at 459.489 kb on - strand at 459.489 kb on - strand at 459.489 kb on - strand at 459.517 kb on + strand at 459.517 kb on + strand at 459.517 kb on + strand at 459.517 kb on + strand at 459.518 kb on - strand at 459.518 kb on - strand at 459.518 kb on - strand at 459.614 kb on - strand, within COO64_RS02205 at 459.614 kb on - strand, within COO64_RS02205 at 459.812 kb on + strand at 459.812 kb on + strand at 459.813 kb on - strand at 459.813 kb on - strand at 459.813 kb on - strand at 459.829 kb on + strand at 459.829 kb on + strand at 459.830 kb on - strand at 459.830 kb on - strand at 459.830 kb on - strand at 459.830 kb on - strand at 459.860 kb on + strand at 459.860 kb on + strand at 459.931 kb on + strand at 459.932 kb on - strand at 459.932 kb on - strand at 459.932 kb on - strand at 459.932 kb on - strand at 460.007 kb on - strand, within trkA at 460.007 kb on - strand, within trkA at 460.007 kb on - strand, within trkA at 460.007 kb on - strand, within trkA at 460.184 kb on + strand, within trkA at 460.184 kb on + strand, within trkA at 460.184 kb on + strand, within trkA at 460.184 kb on + strand, within trkA at 460.184 kb on + strand, within trkA at 460.185 kb on - strand, within trkA at 460.185 kb on - strand, within trkA at 460.352 kb on - strand, within trkA
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 457,880 - +1.0 457,902 - +0.3 457,915 + -1.2 457,915 + -0.3 457,915 + +0.3 457,961 + +0.5 457,961 + +0.7 457,961 + -1.3 457,962 - -0.4 457,962 - -0.2 458,091 + COO64_RS02195 0.32 +0.8 458,091 + COO64_RS02195 0.32 -0.3 458,091 + COO64_RS02195 0.32 +0.2 458,092 - COO64_RS02195 0.32 -0.1 458,299 + COO64_RS02195 0.69 +0.5 458,300 - COO64_RS02195 0.70 -1.2 458,472 + -0.1 458,472 + +0.1 458,472 + -0.2 458,473 - -0.1 458,858 - COO64_RS02200 0.40 +0.1 458,858 - COO64_RS02200 0.40 -0.1 458,897 + COO64_RS02200 0.44 -2.4 458,897 + COO64_RS02200 0.44 -0.9 458,897 + COO64_RS02200 0.44 +0.1 458,897 + COO64_RS02200 0.44 -0.6 458,897 + COO64_RS02200 0.44 -1.7 458,898 - COO64_RS02200 0.44 -1.1 458,898 - COO64_RS02200 0.44 +0.2 458,898 - COO64_RS02200 0.44 -2.3 458,898 - COO64_RS02200 0.44 -0.5 458,898 - COO64_RS02200 0.44 -1.4 458,898 - COO64_RS02200 0.44 +2.1 459,257 + COO64_RS02200 0.84 -0.5 459,257 + COO64_RS02200 0.84 -0.9 459,257 + COO64_RS02200 0.84 -0.9 459,257 + COO64_RS02200 0.84 +1.0 459,257 + COO64_RS02200 0.84 +1.1 459,311 + -0.1 459,311 + -0.6 459,311 + +0.1 459,311 + +0.3 459,311 + +0.1 459,311 + +0.5 459,311 + -0.8 459,311 + -1.7 459,312 - -0.3 459,312 - +0.5 459,312 - -0.3 459,312 - -2.4 459,312 - -1.1 459,312 - -0.3 459,335 + -0.1 459,335 + -0.1 459,336 - -1.2 459,336 - -1.8 459,336 - -0.1 459,336 - -0.4 459,390 - +0.5 459,390 - -1.0 459,390 - -1.5 459,489 - +0.6 459,489 - +0.7 459,489 - -1.8 459,489 - +0.8 459,517 + -0.7 459,517 + -1.7 459,517 + -0.2 459,517 + -0.7 459,518 - -0.7 459,518 - +0.1 459,518 - -1.2 459,614 - COO64_RS02205 0.41 +0.5 459,614 - COO64_RS02205 0.41 -1.2 459,812 + +0.1 459,812 + +1.6 459,813 - -0.7 459,813 - -1.0 459,813 - +0.2 459,829 + -0.1 459,829 + +1.0 459,830 - -0.9 459,830 - +0.2 459,830 - -0.1 459,830 - -0.0 459,860 + -0.1 459,860 + +2.1 459,931 + +0.1 459,932 - -0.5 459,932 - -0.4 459,932 - +0.8 459,932 - -1.2 460,007 - trkA COO64_RS02210 0.14 +0.1 460,007 - trkA COO64_RS02210 0.14 -2.8 460,007 - trkA COO64_RS02210 0.14 -0.3 460,007 - trkA COO64_RS02210 0.14 +0.5 460,184 + trkA COO64_RS02210 0.27 -0.0 460,184 + trkA COO64_RS02210 0.27 -0.4 460,184 + trkA COO64_RS02210 0.27 -0.2 460,184 + trkA COO64_RS02210 0.27 +0.3 460,184 + trkA COO64_RS02210 0.27 -0.6 460,185 - trkA COO64_RS02210 0.27 +0.3 460,185 - trkA COO64_RS02210 0.27 +0.2 460,352 - trkA COO64_RS02210 0.39 -0.3
Or see this region's nucleotide sequence