Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS02065 and COO64_RS02070 are separated by 9 nucleotides COO64_RS02070 and rsmG are separated by 18 nucleotides rsmG and mnmG overlap by 4 nucleotides
COO64_RS02065: COO64_RS02065 - ParB/RepB/Spo0J family partition protein, at 426,132 to 427,004
_RS02065
COO64_RS02070: COO64_RS02070 - ParA family protein, at 427,014 to 427,805
_RS02070
COO64_RS02075: rsmG - 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG, at 427,824 to 428,468
rsmG
COO64_RS02080: mnmG - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 428,465 to 430,357
mnmG
Position (kb) 427
428
429 Strain fitness (log2 ratio) -2
-1
0
1
2 at 426.859 kb on + strand, within COO64_RS02065 at 426.859 kb on + strand, within COO64_RS02065 at 426.859 kb on + strand, within COO64_RS02065 at 426.860 kb on - strand, within COO64_RS02065 at 426.860 kb on - strand, within COO64_RS02065 at 426.860 kb on - strand, within COO64_RS02065 at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.015 kb on + strand at 427.016 kb on - strand at 427.016 kb on - strand at 427.016 kb on - strand at 427.016 kb on - strand at 427.111 kb on + strand, within COO64_RS02070 at 427.111 kb on + strand, within COO64_RS02070 at 427.112 kb on - strand, within COO64_RS02070 at 427.180 kb on + strand, within COO64_RS02070 at 427.180 kb on + strand, within COO64_RS02070 at 427.180 kb on + strand, within COO64_RS02070 at 427.180 kb on + strand, within COO64_RS02070 at 427.181 kb on - strand, within COO64_RS02070 at 427.181 kb on - strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.249 kb on + strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.250 kb on - strand, within COO64_RS02070 at 427.351 kb on + strand, within COO64_RS02070 at 427.351 kb on + strand, within COO64_RS02070 at 427.351 kb on + strand, within COO64_RS02070 at 427.352 kb on - strand, within COO64_RS02070 at 427.352 kb on - strand, within COO64_RS02070 at 427.444 kb on + strand, within COO64_RS02070 at 427.444 kb on + strand, within COO64_RS02070 at 427.444 kb on + strand, within COO64_RS02070 at 427.444 kb on + strand, within COO64_RS02070 at 427.444 kb on + strand, within COO64_RS02070 at 427.444 kb on + strand, within COO64_RS02070 at 427.445 kb on - strand, within COO64_RS02070 at 427.445 kb on - strand, within COO64_RS02070 at 427.445 kb on - strand, within COO64_RS02070 at 427.445 kb on - strand, within COO64_RS02070 at 427.450 kb on + strand, within COO64_RS02070 at 427.451 kb on - strand, within COO64_RS02070 at 427.625 kb on - strand, within COO64_RS02070 at 427.625 kb on - strand, within COO64_RS02070 at 427.625 kb on - strand, within COO64_RS02070 at 427.625 kb on - strand, within COO64_RS02070 at 427.625 kb on - strand, within COO64_RS02070 at 427.745 kb on + strand at 427.746 kb on - strand at 427.794 kb on + strand at 427.795 kb on - strand at 427.799 kb on + strand at 427.843 kb on - strand at 427.923 kb on + strand, within rsmG at 427.923 kb on + strand, within rsmG at 427.924 kb on - strand, within rsmG at 428.121 kb on + strand, within rsmG at 428.121 kb on + strand, within rsmG at 428.332 kb on + strand, within rsmG at 428.332 kb on + strand, within rsmG at 428.333 kb on - strand, within rsmG at 428.333 kb on - strand, within rsmG
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 426,859 + COO64_RS02065 0.83 -0.1 426,859 + COO64_RS02065 0.83 +0.5 426,859 + COO64_RS02065 0.83 -1.2 426,860 - COO64_RS02065 0.83 +1.9 426,860 - COO64_RS02065 0.83 -0.7 426,860 - COO64_RS02065 0.83 -0.4 427,015 + -1.0 427,015 + -0.5 427,015 + +0.6 427,015 + -1.1 427,015 + -0.6 427,015 + -0.4 427,015 + +0.6 427,015 + -0.3 427,015 + -1.2 427,015 + -0.1 427,016 - +0.3 427,016 - -0.3 427,016 - -2.4 427,016 - -0.5 427,111 + COO64_RS02070 0.12 -0.1 427,111 + COO64_RS02070 0.12 -0.4 427,112 - COO64_RS02070 0.12 +0.4 427,180 + COO64_RS02070 0.21 +0.9 427,180 + COO64_RS02070 0.21 +0.1 427,180 + COO64_RS02070 0.21 -0.7 427,180 + COO64_RS02070 0.21 +0.6 427,181 - COO64_RS02070 0.21 -0.5 427,181 - COO64_RS02070 0.21 -0.9 427,249 + COO64_RS02070 0.30 -0.5 427,249 + COO64_RS02070 0.30 +0.9 427,249 + COO64_RS02070 0.30 -1.0 427,249 + COO64_RS02070 0.30 +2.1 427,249 + COO64_RS02070 0.30 +1.3 427,249 + COO64_RS02070 0.30 +0.5 427,249 + COO64_RS02070 0.30 -0.4 427,250 - COO64_RS02070 0.30 -0.4 427,250 - COO64_RS02070 0.30 -0.4 427,250 - COO64_RS02070 0.30 +0.0 427,250 - COO64_RS02070 0.30 +0.7 427,250 - COO64_RS02070 0.30 -0.5 427,250 - COO64_RS02070 0.30 -1.2 427,250 - COO64_RS02070 0.30 +1.2 427,250 - COO64_RS02070 0.30 -0.9 427,250 - COO64_RS02070 0.30 +1.8 427,250 - COO64_RS02070 0.30 +0.5 427,351 + COO64_RS02070 0.43 -0.8 427,351 + COO64_RS02070 0.43 -1.7 427,351 + COO64_RS02070 0.43 -0.9 427,352 - COO64_RS02070 0.43 -1.1 427,352 - COO64_RS02070 0.43 -1.1 427,444 + COO64_RS02070 0.54 -0.7 427,444 + COO64_RS02070 0.54 +0.3 427,444 + COO64_RS02070 0.54 +1.1 427,444 + COO64_RS02070 0.54 -1.3 427,444 + COO64_RS02070 0.54 -0.5 427,444 + COO64_RS02070 0.54 -1.4 427,445 - COO64_RS02070 0.54 -0.4 427,445 - COO64_RS02070 0.54 -0.1 427,445 - COO64_RS02070 0.54 -0.0 427,445 - COO64_RS02070 0.54 +0.1 427,450 + COO64_RS02070 0.55 +1.3 427,451 - COO64_RS02070 0.55 +0.1 427,625 - COO64_RS02070 0.77 -0.3 427,625 - COO64_RS02070 0.77 +0.2 427,625 - COO64_RS02070 0.77 +0.5 427,625 - COO64_RS02070 0.77 -0.9 427,625 - COO64_RS02070 0.77 +0.5 427,745 + -1.4 427,746 - +0.5 427,794 + -0.4 427,795 - -1.1 427,799 + -0.4 427,843 - +0.5 427,923 + rsmG COO64_RS02075 0.15 -1.7 427,923 + rsmG COO64_RS02075 0.15 -0.5 427,924 - rsmG COO64_RS02075 0.16 -0.1 428,121 + rsmG COO64_RS02075 0.46 +0.1 428,121 + rsmG COO64_RS02075 0.46 -1.1 428,332 + rsmG COO64_RS02075 0.79 +0.3 428,332 + rsmG COO64_RS02075 0.79 +0.1 428,333 - rsmG COO64_RS02075 0.79 +0.1 428,333 - rsmG COO64_RS02075 0.79 -0.6
Or see this region's nucleotide sequence