Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcnB and hcnA overlap by 4 nucleotides hcnA and glmS are separated by 161 nucleotides
COO64_RS01995: hcnB - cyanide-forming glycine dehydrogenase subunit HcnB, at 412,706 to 414,100
hcnB
COO64_RS02000: hcnA - cyanide-forming glycine dehydrogenase subunit HcnA, at 414,097 to 414,411
hcnA
COO64_RS02005: glmS - glutamine--fructose-6-phosphate transaminase (isomerizing), at 414,573 to 416,408
glmS
Position (kb) 414
415 Strain fitness (log2 ratio) -2
-1
0
1
2 at 413.097 kb on - strand, within hcnB at 413.097 kb on - strand, within hcnB at 413.097 kb on - strand, within hcnB at 413.097 kb on - strand, within hcnB at 413.097 kb on - strand, within hcnB at 413.097 kb on - strand, within hcnB at 413.241 kb on + strand, within hcnB at 413.241 kb on + strand, within hcnB at 413.242 kb on - strand, within hcnB at 413.242 kb on - strand, within hcnB at 413.284 kb on + strand, within hcnB at 413.285 kb on - strand, within hcnB at 413.285 kb on - strand, within hcnB at 413.303 kb on - strand, within hcnB at 413.380 kb on - strand, within hcnB at 413.442 kb on + strand, within hcnB at 413.443 kb on - strand, within hcnB at 413.443 kb on - strand, within hcnB at 413.443 kb on - strand, within hcnB at 413.443 kb on - strand, within hcnB at 413.443 kb on - strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.445 kb on + strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.446 kb on - strand, within hcnB at 413.472 kb on + strand, within hcnB at 413.472 kb on + strand, within hcnB at 413.472 kb on + strand, within hcnB at 413.473 kb on - strand, within hcnB at 413.473 kb on - strand, within hcnB at 413.473 kb on - strand, within hcnB at 413.473 kb on - strand, within hcnB at 413.473 kb on - strand, within hcnB at 413.565 kb on + strand, within hcnB at 413.565 kb on + strand, within hcnB at 413.566 kb on - strand, within hcnB at 413.566 kb on - strand, within hcnB at 413.566 kb on - strand, within hcnB at 413.566 kb on - strand, within hcnB at 413.566 kb on - strand, within hcnB at 413.566 kb on - strand, within hcnB at 413.648 kb on - strand, within hcnB at 413.648 kb on - strand, within hcnB at 413.738 kb on + strand, within hcnB at 413.738 kb on + strand, within hcnB at 413.739 kb on - strand, within hcnB at 413.739 kb on - strand, within hcnB at 413.739 kb on - strand, within hcnB at 413.739 kb on - strand, within hcnB at 413.739 kb on - strand, within hcnB at 413.739 kb on - strand, within hcnB at 413.926 kb on - strand, within hcnB at 413.941 kb on - strand, within hcnB at 413.941 kb on - strand, within hcnB at 414.148 kb on - strand, within hcnA at 414.286 kb on + strand, within hcnA at 414.286 kb on + strand, within hcnA at 414.385 kb on - strand at 414.385 kb on - strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.388 kb on + strand at 414.389 kb on - strand at 414.389 kb on - strand at 414.389 kb on - strand at 414.389 kb on - strand at 414.411 kb on + strand at 414.411 kb on + strand at 414.411 kb on + strand at 414.411 kb on + strand at 414.411 kb on + strand at 414.412 kb on - strand at 414.412 kb on - strand at 414.452 kb on + strand at 414.453 kb on - strand at 414.453 kb on - strand at 414.453 kb on - strand at 414.453 kb on - strand at 414.453 kb on - strand at 414.453 kb on - strand at 414.474 kb on + strand at 414.474 kb on + strand at 414.475 kb on - strand at 414.475 kb on - strand at 414.475 kb on - strand at 414.475 kb on - strand at 414.475 kb on - strand at 414.475 kb on - strand at 414.491 kb on + strand at 414.491 kb on + strand at 414.491 kb on + strand at 414.491 kb on + strand at 414.492 kb on - strand at 414.492 kb on - strand at 414.492 kb on - strand at 414.492 kb on - strand at 414.492 kb on - strand at 414.558 kb on + strand at 414.559 kb on - strand at 414.559 kb on - strand at 414.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 413,097 - hcnB COO64_RS01995 0.28 -0.4 413,097 - hcnB COO64_RS01995 0.28 +0.1 413,097 - hcnB COO64_RS01995 0.28 +0.4 413,097 - hcnB COO64_RS01995 0.28 -0.7 413,097 - hcnB COO64_RS01995 0.28 -0.8 413,097 - hcnB COO64_RS01995 0.28 +0.0 413,241 + hcnB COO64_RS01995 0.38 -0.9 413,241 + hcnB COO64_RS01995 0.38 -0.5 413,242 - hcnB COO64_RS01995 0.38 -0.9 413,242 - hcnB COO64_RS01995 0.38 -1.3 413,284 + hcnB COO64_RS01995 0.41 +0.3 413,285 - hcnB COO64_RS01995 0.42 -0.4 413,285 - hcnB COO64_RS01995 0.42 -0.1 413,303 - hcnB COO64_RS01995 0.43 -0.7 413,380 - hcnB COO64_RS01995 0.48 -0.2 413,442 + hcnB COO64_RS01995 0.53 -0.6 413,443 - hcnB COO64_RS01995 0.53 -0.7 413,443 - hcnB COO64_RS01995 0.53 +0.8 413,443 - hcnB COO64_RS01995 0.53 +0.2 413,443 - hcnB COO64_RS01995 0.53 +1.4 413,443 - hcnB COO64_RS01995 0.53 +0.1 413,445 + hcnB COO64_RS01995 0.53 -0.5 413,445 + hcnB COO64_RS01995 0.53 -1.6 413,445 + hcnB COO64_RS01995 0.53 +0.4 413,445 + hcnB COO64_RS01995 0.53 -0.4 413,445 + hcnB COO64_RS01995 0.53 -0.7 413,445 + hcnB COO64_RS01995 0.53 +0.5 413,445 + hcnB COO64_RS01995 0.53 +0.8 413,445 + hcnB COO64_RS01995 0.53 -0.9 413,445 + hcnB COO64_RS01995 0.53 -0.2 413,445 + hcnB COO64_RS01995 0.53 +0.8 413,445 + hcnB COO64_RS01995 0.53 -0.9 413,445 + hcnB COO64_RS01995 0.53 +0.2 413,445 + hcnB COO64_RS01995 0.53 +0.3 413,446 - hcnB COO64_RS01995 0.53 +0.7 413,446 - hcnB COO64_RS01995 0.53 +0.2 413,446 - hcnB COO64_RS01995 0.53 -1.4 413,446 - hcnB COO64_RS01995 0.53 -2.2 413,446 - hcnB COO64_RS01995 0.53 +1.1 413,446 - hcnB COO64_RS01995 0.53 +0.7 413,446 - hcnB COO64_RS01995 0.53 -0.3 413,446 - hcnB COO64_RS01995 0.53 -0.3 413,446 - hcnB COO64_RS01995 0.53 +0.3 413,472 + hcnB COO64_RS01995 0.55 -0.2 413,472 + hcnB COO64_RS01995 0.55 -0.8 413,472 + hcnB COO64_RS01995 0.55 +0.9 413,473 - hcnB COO64_RS01995 0.55 -0.2 413,473 - hcnB COO64_RS01995 0.55 -1.2 413,473 - hcnB COO64_RS01995 0.55 +0.8 413,473 - hcnB COO64_RS01995 0.55 -0.7 413,473 - hcnB COO64_RS01995 0.55 -0.9 413,565 + hcnB COO64_RS01995 0.62 -1.6 413,565 + hcnB COO64_RS01995 0.62 -0.7 413,566 - hcnB COO64_RS01995 0.62 +0.2 413,566 - hcnB COO64_RS01995 0.62 +1.5 413,566 - hcnB COO64_RS01995 0.62 -1.2 413,566 - hcnB COO64_RS01995 0.62 -1.4 413,566 - hcnB COO64_RS01995 0.62 -0.6 413,566 - hcnB COO64_RS01995 0.62 +0.7 413,648 - hcnB COO64_RS01995 0.68 +1.1 413,648 - hcnB COO64_RS01995 0.68 +0.3 413,738 + hcnB COO64_RS01995 0.74 -0.4 413,738 + hcnB COO64_RS01995 0.74 +0.7 413,739 - hcnB COO64_RS01995 0.74 +0.4 413,739 - hcnB COO64_RS01995 0.74 +0.1 413,739 - hcnB COO64_RS01995 0.74 -1.5 413,739 - hcnB COO64_RS01995 0.74 +0.3 413,739 - hcnB COO64_RS01995 0.74 +0.5 413,739 - hcnB COO64_RS01995 0.74 +0.1 413,926 - hcnB COO64_RS01995 0.87 +0.7 413,941 - hcnB COO64_RS01995 0.89 -0.5 413,941 - hcnB COO64_RS01995 0.89 -0.5 414,148 - hcnA COO64_RS02000 0.16 -0.2 414,286 + hcnA COO64_RS02000 0.60 -2.0 414,286 + hcnA COO64_RS02000 0.60 -1.7 414,385 - -0.3 414,385 - -0.9 414,388 + -0.9 414,388 + -1.0 414,388 + -1.0 414,388 + +1.0 414,388 + +0.7 414,388 + +1.0 414,388 + -1.0 414,388 + -0.3 414,388 + +0.2 414,388 + +0.3 414,389 - +0.1 414,389 - -0.3 414,389 - -0.4 414,389 - -1.0 414,411 + +0.1 414,411 + -0.2 414,411 + -0.3 414,411 + +0.0 414,411 + +0.2 414,412 - -1.4 414,412 - +2.2 414,452 + +1.7 414,453 - -0.2 414,453 - +0.4 414,453 - -0.4 414,453 - -0.6 414,453 - -0.2 414,453 - -0.3 414,474 + +0.0 414,474 + +0.5 414,475 - -1.1 414,475 - +0.0 414,475 - -0.5 414,475 - -0.9 414,475 - +0.2 414,475 - -1.2 414,491 + -0.7 414,491 + +0.2 414,491 + -0.1 414,491 + -0.6 414,492 - -0.1 414,492 - +2.1 414,492 - +0.8 414,492 - +0.6 414,492 - +0.9 414,558 + -1.1 414,559 - -0.2 414,559 - +0.7 414,559 - -0.2
Or see this region's nucleotide sequence