Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapF and COO64_RS01265 are separated by 13 nucleotides COO64_RS01265 and xerC overlap by 1 nucleotides xerC and COO64_RS01275 overlap by 4 nucleotides COO64_RS01275 and sutA are separated by 148 nucleotides
COO64_RS01260: dapF - diaminopimelate epimerase, at 264,122 to 264,952
dapF
COO64_RS01265: COO64_RS01265 - DUF484 family protein, at 264,966 to 265,703
_RS01265
COO64_RS01270: xerC - tyrosine recombinase XerC, at 265,703 to 266,602
xerC
COO64_RS01275: COO64_RS01275 - HAD family hydrolase, at 266,599 to 267,294
_RS01275
COO64_RS01280: sutA - transcriptional regulator SutA, at 267,443 to 267,766
sutA
Position (kb) 265
266
267 Strain fitness (log2 ratio) -2
-1
0
1
2 at 264.948 kb on + strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.949 kb on - strand at 264.958 kb on + strand at 264.958 kb on + strand at 264.958 kb on + strand at 264.958 kb on + strand at 265.011 kb on - strand at 265.060 kb on - strand, within COO64_RS01265 at 265.188 kb on - strand, within COO64_RS01265 at 265.188 kb on - strand, within COO64_RS01265 at 265.455 kb on - strand, within COO64_RS01265 at 265.573 kb on + strand, within COO64_RS01265 at 265.574 kb on - strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.605 kb on + strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.606 kb on - strand, within COO64_RS01265 at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.638 kb on + strand at 265.639 kb on - strand at 265.639 kb on - strand at 265.639 kb on - strand at 265.639 kb on - strand at 265.639 kb on - strand at 265.639 kb on - strand at 265.639 kb on - strand at 265.663 kb on + strand at 265.663 kb on + strand at 265.664 kb on - strand at 265.664 kb on - strand at 265.846 kb on - strand, within xerC at 265.943 kb on + strand, within xerC at 265.943 kb on + strand, within xerC at 265.944 kb on - strand, within xerC at 266.123 kb on + strand, within xerC at 266.123 kb on + strand, within xerC at 266.123 kb on + strand, within xerC at 266.123 kb on + strand, within xerC at 266.124 kb on - strand, within xerC at 266.124 kb on - strand, within xerC at 266.124 kb on - strand, within xerC at 266.196 kb on + strand, within xerC at 266.197 kb on - strand, within xerC at 266.368 kb on - strand, within xerC at 266.472 kb on + strand, within xerC at 266.472 kb on + strand, within xerC at 266.473 kb on - strand, within xerC at 266.473 kb on - strand, within xerC at 266.473 kb on - strand, within xerC at 266.473 kb on - strand, within xerC at 266.473 kb on - strand, within xerC at 266.516 kb on + strand at 266.516 kb on + strand at 266.517 kb on - strand at 266.517 kb on - strand at 266.517 kb on - strand at 266.517 kb on - strand at 266.517 kb on - strand at 266.517 kb on - strand at 266.552 kb on + strand at 266.552 kb on + strand at 266.552 kb on + strand at 266.552 kb on + strand at 266.553 kb on - strand at 266.553 kb on - strand at 266.553 kb on - strand at 266.553 kb on - strand at 266.553 kb on - strand at 266.553 kb on - strand at 266.584 kb on + strand at 266.584 kb on + strand at 266.584 kb on + strand at 266.584 kb on + strand at 266.584 kb on + strand at 266.585 kb on - strand at 266.585 kb on - strand at 266.585 kb on - strand at 266.740 kb on + strand, within COO64_RS01275 at 266.741 kb on - strand, within COO64_RS01275 at 266.741 kb on - strand, within COO64_RS01275 at 266.741 kb on - strand, within COO64_RS01275 at 266.962 kb on + strand, within COO64_RS01275 at 266.962 kb on + strand, within COO64_RS01275 at 266.962 kb on + strand, within COO64_RS01275 at 266.962 kb on + strand, within COO64_RS01275 at 266.962 kb on + strand, within COO64_RS01275 at 266.962 kb on + strand, within COO64_RS01275 at 266.963 kb on - strand, within COO64_RS01275 at 266.963 kb on - strand, within COO64_RS01275 at 267.022 kb on + strand, within COO64_RS01275 at 267.022 kb on + strand, within COO64_RS01275 at 267.023 kb on - strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.053 kb on + strand, within COO64_RS01275 at 267.054 kb on - strand, within COO64_RS01275 at 267.054 kb on - strand, within COO64_RS01275 at 267.054 kb on - strand, within COO64_RS01275 at 267.054 kb on - strand, within COO64_RS01275 at 267.054 kb on - strand, within COO64_RS01275 at 267.054 kb on - strand, within COO64_RS01275 at 267.059 kb on + strand, within COO64_RS01275 at 267.059 kb on + strand, within COO64_RS01275 at 267.060 kb on - strand, within COO64_RS01275 at 267.128 kb on + strand, within COO64_RS01275 at 267.129 kb on - strand, within COO64_RS01275 at 267.129 kb on - strand, within COO64_RS01275 at 267.129 kb on - strand, within COO64_RS01275 at 267.129 kb on - strand, within COO64_RS01275 at 267.129 kb on - strand, within COO64_RS01275 at 267.292 kb on + strand at 267.367 kb on + strand at 267.368 kb on - strand at 267.389 kb on + strand at 267.389 kb on + strand at 267.390 kb on - strand at 267.390 kb on - strand at 267.390 kb on - strand at 267.390 kb on - strand at 267.449 kb on - strand at 267.449 kb on - strand at 267.449 kb on - strand at 267.462 kb on + strand at 267.462 kb on + strand at 267.462 kb on + strand at 267.462 kb on + strand at 267.462 kb on + strand at 267.462 kb on + strand at 267.463 kb on - strand at 267.463 kb on - strand at 267.463 kb on - strand at 267.463 kb on - strand at 267.463 kb on - strand at 267.463 kb on - strand at 267.463 kb on - strand at 267.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 264,948 + -0.3 264,948 + -0.3 264,948 + +0.7 264,948 + +1.3 264,948 + -0.5 264,949 - +0.6 264,958 + -0.9 264,958 + +0.6 264,958 + -0.0 264,958 + -0.6 265,011 - +1.1 265,060 - COO64_RS01265 0.13 -0.2 265,188 - COO64_RS01265 0.30 -0.6 265,188 - COO64_RS01265 0.30 -0.3 265,455 - COO64_RS01265 0.66 -0.1 265,573 + COO64_RS01265 0.82 -0.9 265,574 - COO64_RS01265 0.82 -0.6 265,605 + COO64_RS01265 0.87 -0.2 265,605 + COO64_RS01265 0.87 +0.4 265,605 + COO64_RS01265 0.87 -0.7 265,605 + COO64_RS01265 0.87 +0.2 265,605 + COO64_RS01265 0.87 -0.2 265,605 + COO64_RS01265 0.87 +0.1 265,605 + COO64_RS01265 0.87 -0.0 265,606 - COO64_RS01265 0.87 -0.2 265,606 - COO64_RS01265 0.87 -1.1 265,606 - COO64_RS01265 0.87 +0.6 265,606 - COO64_RS01265 0.87 -0.2 265,606 - COO64_RS01265 0.87 +0.8 265,606 - COO64_RS01265 0.87 +0.5 265,606 - COO64_RS01265 0.87 -0.6 265,606 - COO64_RS01265 0.87 +1.0 265,606 - COO64_RS01265 0.87 +0.4 265,638 + -0.4 265,638 + -0.0 265,638 + -0.3 265,638 + -1.8 265,638 + +1.3 265,638 + +0.2 265,638 + -0.3 265,638 + +0.9 265,638 + -0.1 265,639 - -0.7 265,639 - +0.5 265,639 - +0.1 265,639 - -0.1 265,639 - -1.2 265,639 - -0.7 265,639 - +0.3 265,663 + -0.7 265,663 + -0.6 265,664 - -1.4 265,664 - +1.3 265,846 - xerC COO64_RS01270 0.16 +0.3 265,943 + xerC COO64_RS01270 0.27 +1.3 265,943 + xerC COO64_RS01270 0.27 +0.3 265,944 - xerC COO64_RS01270 0.27 -0.5 266,123 + xerC COO64_RS01270 0.47 +2.1 266,123 + xerC COO64_RS01270 0.47 -1.6 266,123 + xerC COO64_RS01270 0.47 -1.1 266,123 + xerC COO64_RS01270 0.47 -0.9 266,124 - xerC COO64_RS01270 0.47 -0.3 266,124 - xerC COO64_RS01270 0.47 -0.5 266,124 - xerC COO64_RS01270 0.47 -1.0 266,196 + xerC COO64_RS01270 0.55 -0.7 266,197 - xerC COO64_RS01270 0.55 -1.1 266,368 - xerC COO64_RS01270 0.74 -0.4 266,472 + xerC COO64_RS01270 0.85 +0.1 266,472 + xerC COO64_RS01270 0.85 -0.3 266,473 - xerC COO64_RS01270 0.86 -0.6 266,473 - xerC COO64_RS01270 0.86 +0.5 266,473 - xerC COO64_RS01270 0.86 +0.6 266,473 - xerC COO64_RS01270 0.86 -0.5 266,473 - xerC COO64_RS01270 0.86 -1.4 266,516 + -1.5 266,516 + -1.4 266,517 - -2.6 266,517 - -0.9 266,517 - +1.1 266,517 - -0.6 266,517 - -2.2 266,517 - -0.4 266,552 + -0.7 266,552 + +0.6 266,552 + -0.4 266,552 + -0.5 266,553 - +0.5 266,553 - +0.3 266,553 - -0.8 266,553 - +0.1 266,553 - -1.0 266,553 - +0.4 266,584 + -0.9 266,584 + -0.3 266,584 + -0.5 266,584 + -0.1 266,584 + +0.2 266,585 - +1.0 266,585 - -0.4 266,585 - +0.2 266,740 + COO64_RS01275 0.20 -0.1 266,741 - COO64_RS01275 0.20 -0.2 266,741 - COO64_RS01275 0.20 +0.3 266,741 - COO64_RS01275 0.20 -0.1 266,962 + COO64_RS01275 0.52 +0.2 266,962 + COO64_RS01275 0.52 -1.0 266,962 + COO64_RS01275 0.52 +0.8 266,962 + COO64_RS01275 0.52 +0.5 266,962 + COO64_RS01275 0.52 +0.9 266,962 + COO64_RS01275 0.52 -1.4 266,963 - COO64_RS01275 0.52 +0.8 266,963 - COO64_RS01275 0.52 -1.4 267,022 + COO64_RS01275 0.61 +0.4 267,022 + COO64_RS01275 0.61 -0.8 267,023 - COO64_RS01275 0.61 -0.6 267,053 + COO64_RS01275 0.65 -0.3 267,053 + COO64_RS01275 0.65 -0.7 267,053 + COO64_RS01275 0.65 -0.1 267,053 + COO64_RS01275 0.65 +0.7 267,053 + COO64_RS01275 0.65 -0.2 267,053 + COO64_RS01275 0.65 -0.8 267,053 + COO64_RS01275 0.65 +0.1 267,053 + COO64_RS01275 0.65 +2.1 267,053 + COO64_RS01275 0.65 +1.7 267,053 + COO64_RS01275 0.65 +0.6 267,054 - COO64_RS01275 0.65 -0.7 267,054 - COO64_RS01275 0.65 -0.9 267,054 - COO64_RS01275 0.65 +0.2 267,054 - COO64_RS01275 0.65 -0.4 267,054 - COO64_RS01275 0.65 -1.3 267,054 - COO64_RS01275 0.65 +0.2 267,059 + COO64_RS01275 0.66 -0.0 267,059 + COO64_RS01275 0.66 -0.1 267,060 - COO64_RS01275 0.66 -0.4 267,128 + COO64_RS01275 0.76 +0.8 267,129 - COO64_RS01275 0.76 +0.3 267,129 - COO64_RS01275 0.76 +1.1 267,129 - COO64_RS01275 0.76 +0.0 267,129 - COO64_RS01275 0.76 +0.2 267,129 - COO64_RS01275 0.76 +0.5 267,292 + -0.1 267,367 + +0.8 267,368 - +1.1 267,389 + -0.2 267,389 + +0.1 267,390 - -0.0 267,390 - -0.0 267,390 - +0.3 267,390 - +0.1 267,449 - +0.3 267,449 - +0.6 267,449 - -0.1 267,462 + +0.5 267,462 + -0.5 267,462 + +0.7 267,462 + +0.9 267,462 + +0.4 267,462 + +1.2 267,463 - -1.5 267,463 - +0.3 267,463 - +0.9 267,463 - -0.1 267,463 - +0.6 267,463 - +0.4 267,463 - -0.3 267,463 - +0.1
Or see this region's nucleotide sequence