Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS01240 and cyaY overlap by 1 nucleotides cyaY and COO64_RS01250 are separated by 195 nucleotides COO64_RS01250 and lysA are separated by 9 nucleotides lysA and dapF are separated by 4 nucleotides dapF and COO64_RS01265 are separated by 13 nucleotides
COO64_RS01240: COO64_RS01240 - DUF1289 domain-containing protein, at 261,974 to 262,195
_RS01240
COO64_RS01245: cyaY - iron donor protein CyaY, at 262,195 to 262,527
cyaY
COO64_RS01250: COO64_RS01250 - LPS translocon maturation chaperone LptM, at 262,723 to 262,860
_RS01250
COO64_RS01255: lysA - diaminopimelate decarboxylase, at 262,870 to 264,117
lysA
COO64_RS01260: dapF - diaminopimelate epimerase, at 264,122 to 264,952
dapF
COO64_RS01265: COO64_RS01265 - DUF484 family protein, at 264,966 to 265,703
_RS01265
Position (kb) 262
263
264
265 Strain fitness (log2 ratio) -2
-1
0
1 at 261.870 kb on + strand at 261.870 kb on + strand at 261.871 kb on - strand at 261.871 kb on - strand at 261.872 kb on + strand at 261.872 kb on + strand at 261.873 kb on - strand at 261.873 kb on - strand at 261.873 kb on - strand at 261.938 kb on + strand at 261.938 kb on + strand at 261.939 kb on - strand at 261.939 kb on - strand at 261.939 kb on - strand at 261.951 kb on + strand at 261.951 kb on + strand at 261.951 kb on + strand at 261.952 kb on - strand at 261.952 kb on - strand at 261.952 kb on - strand at 261.955 kb on + strand at 261.955 kb on + strand at 261.955 kb on + strand at 261.955 kb on + strand at 261.955 kb on + strand at 261.955 kb on + strand at 261.955 kb on + strand at 261.956 kb on - strand at 261.956 kb on - strand at 261.956 kb on - strand at 261.963 kb on - strand at 261.975 kb on + strand at 261.975 kb on + strand at 261.975 kb on + strand at 261.976 kb on - strand at 261.976 kb on - strand at 261.976 kb on - strand at 262.161 kb on + strand, within COO64_RS01240 at 262.161 kb on + strand, within COO64_RS01240 at 262.161 kb on + strand, within COO64_RS01240 at 262.310 kb on + strand, within cyaY at 262.311 kb on - strand, within cyaY at 262.573 kb on + strand at 262.573 kb on + strand at 262.573 kb on + strand at 262.573 kb on + strand at 262.574 kb on - strand at 262.574 kb on - strand at 262.574 kb on - strand at 262.659 kb on + strand at 262.659 kb on + strand at 262.659 kb on + strand at 262.660 kb on - strand at 262.660 kb on - strand at 262.682 kb on + strand at 262.684 kb on + strand at 262.684 kb on + strand at 262.684 kb on + strand at 262.685 kb on - strand at 262.697 kb on + strand at 262.697 kb on + strand at 262.697 kb on + strand at 262.698 kb on - strand at 262.704 kb on + strand at 262.704 kb on + strand at 262.704 kb on + strand at 262.704 kb on + strand at 262.704 kb on + strand at 262.704 kb on + strand at 262.704 kb on + strand at 262.705 kb on - strand at 262.705 kb on - strand at 262.705 kb on - strand at 262.705 kb on - strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.707 kb on + strand at 262.708 kb on - strand at 262.708 kb on - strand at 262.717 kb on + strand at 262.717 kb on + strand at 262.717 kb on + strand at 262.717 kb on + strand at 262.718 kb on - strand at 262.804 kb on + strand, within COO64_RS01250 at 262.804 kb on + strand, within COO64_RS01250 at 262.858 kb on + strand at 262.859 kb on - strand at 262.957 kb on + strand at 262.957 kb on + strand at 262.957 kb on + strand at 262.995 kb on + strand, within lysA at 262.999 kb on + strand, within lysA at 262.999 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.350 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.413 kb on + strand, within lysA at 263.632 kb on + strand, within lysA at 263.632 kb on + strand, within lysA at 263.698 kb on + strand, within lysA at 263.698 kb on + strand, within lysA at 263.868 kb on + strand, within lysA at 263.868 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.872 kb on + strand, within lysA at 263.977 kb on + strand, within lysA at 263.977 kb on + strand, within lysA at 263.978 kb on - strand, within lysA at 264.001 kb on + strand at 264.001 kb on + strand at 264.001 kb on + strand at 264.001 kb on + strand at 264.001 kb on + strand at 264.001 kb on + strand at 264.001 kb on + strand at 264.002 kb on - strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.948 kb on + strand at 264.949 kb on - strand at 264.958 kb on + strand at 264.958 kb on + strand at 264.958 kb on + strand at 264.958 kb on + strand at 265.011 kb on - strand at 265.060 kb on - strand, within COO64_RS01265
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 261,870 + +0.5 261,870 + +0.6 261,871 - +0.8 261,871 - -0.8 261,872 + -1.0 261,872 + +0.3 261,873 - -1.0 261,873 - +0.6 261,873 - -0.3 261,938 + -0.5 261,938 + +0.1 261,939 - +0.7 261,939 - -0.2 261,939 - -0.2 261,951 + +0.1 261,951 + +0.5 261,951 + +0.8 261,952 - -1.1 261,952 - +0.1 261,952 - +0.7 261,955 + +0.1 261,955 + +0.4 261,955 + -0.2 261,955 + -0.1 261,955 + +0.7 261,955 + +0.4 261,955 + -0.5 261,956 - -0.3 261,956 - +0.2 261,956 - +0.4 261,963 - +0.1 261,975 + +0.7 261,975 + +0.4 261,975 + -0.1 261,976 - +0.5 261,976 - -0.9 261,976 - +0.7 262,161 + COO64_RS01240 0.84 -0.5 262,161 + COO64_RS01240 0.84 +0.1 262,161 + COO64_RS01240 0.84 -0.8 262,310 + cyaY COO64_RS01245 0.35 -0.5 262,311 - cyaY COO64_RS01245 0.35 +0.2 262,573 + +0.5 262,573 + +0.1 262,573 + +0.3 262,573 + -1.0 262,574 - -1.3 262,574 - -0.1 262,574 - +0.3 262,659 + -0.8 262,659 + -0.2 262,659 + -0.0 262,660 - -0.8 262,660 - -0.1 262,682 + +0.6 262,684 + -0.1 262,684 + +0.7 262,684 + -0.2 262,685 - -1.1 262,697 + +1.1 262,697 + -0.5 262,697 + -0.9 262,698 - -0.9 262,704 + -0.5 262,704 + +0.7 262,704 + +1.0 262,704 + +0.6 262,704 + +0.3 262,704 + -0.4 262,704 + +1.1 262,705 - -1.4 262,705 - +1.1 262,705 - -1.4 262,705 - -0.3 262,707 + +0.5 262,707 + -0.7 262,707 + -1.0 262,707 + -0.6 262,707 + +0.2 262,707 + +0.3 262,707 + +0.1 262,707 + -0.6 262,708 - -1.3 262,708 - +0.7 262,717 + -0.7 262,717 + +0.9 262,717 + +0.1 262,717 + -0.7 262,718 - -0.5 262,804 + COO64_RS01250 0.59 +0.7 262,804 + COO64_RS01250 0.59 -0.8 262,858 + -0.1 262,859 - +0.1 262,957 + -0.7 262,957 + +0.7 262,957 + -1.5 262,995 + lysA COO64_RS01255 0.10 -0.5 262,999 + lysA COO64_RS01255 0.10 +0.1 262,999 + lysA COO64_RS01255 0.10 -0.1 263,350 + lysA COO64_RS01255 0.38 -0.3 263,350 + lysA COO64_RS01255 0.38 -1.5 263,350 + lysA COO64_RS01255 0.38 +0.1 263,350 + lysA COO64_RS01255 0.38 -0.9 263,350 + lysA COO64_RS01255 0.38 -0.3 263,350 + lysA COO64_RS01255 0.38 -0.7 263,350 + lysA COO64_RS01255 0.38 -2.3 263,350 + lysA COO64_RS01255 0.38 +1.3 263,350 + lysA COO64_RS01255 0.38 -0.5 263,413 + lysA COO64_RS01255 0.44 -0.9 263,413 + lysA COO64_RS01255 0.44 -1.1 263,413 + lysA COO64_RS01255 0.44 -0.5 263,413 + lysA COO64_RS01255 0.44 -1.3 263,413 + lysA COO64_RS01255 0.44 -0.4 263,413 + lysA COO64_RS01255 0.44 -1.2 263,413 + lysA COO64_RS01255 0.44 -0.4 263,413 + lysA COO64_RS01255 0.44 -2.3 263,632 + lysA COO64_RS01255 0.61 -0.3 263,632 + lysA COO64_RS01255 0.61 +0.8 263,698 + lysA COO64_RS01255 0.66 -0.6 263,698 + lysA COO64_RS01255 0.66 +0.7 263,868 + lysA COO64_RS01255 0.80 +0.5 263,868 + lysA COO64_RS01255 0.80 -0.8 263,872 + lysA COO64_RS01255 0.80 -0.2 263,872 + lysA COO64_RS01255 0.80 -0.6 263,872 + lysA COO64_RS01255 0.80 -0.2 263,872 + lysA COO64_RS01255 0.80 -0.3 263,872 + lysA COO64_RS01255 0.80 +1.1 263,872 + lysA COO64_RS01255 0.80 -0.5 263,872 + lysA COO64_RS01255 0.80 +0.7 263,872 + lysA COO64_RS01255 0.80 -0.7 263,872 + lysA COO64_RS01255 0.80 -0.3 263,977 + lysA COO64_RS01255 0.89 -0.9 263,977 + lysA COO64_RS01255 0.89 +0.1 263,978 - lysA COO64_RS01255 0.89 -0.3 264,001 + -0.5 264,001 + +0.8 264,001 + +0.1 264,001 + +0.6 264,001 + -0.2 264,001 + -1.0 264,001 + -0.1 264,002 - +0.1 264,948 + -0.3 264,948 + -0.3 264,948 + +0.7 264,948 + +1.3 264,948 + -0.5 264,949 - +0.6 264,958 + -0.9 264,958 + +0.6 264,958 + -0.0 264,958 + -0.6 265,011 - +1.1 265,060 - COO64_RS01265 0.13 -0.2
Or see this region's nucleotide sequence