Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rho and trxA are separated by 229 nucleotides trxA and ppx are separated by 241 nucleotides ppx and ppk1 overlap by 14 nucleotides
COO64_RS01065: rho - transcription termination factor Rho, at 227,635 to 228,894
rho
COO64_RS01070: trxA - thioredoxin TrxA, at 229,124 to 229,453
trxA
COO64_RS01075: ppx - exopolyphosphatase, at 229,695 to 231,197
ppx
COO64_RS01080: ppk1 - polyphosphate kinase 1, at 231,184 to 233,415
ppk1
Position (kb) 229
230
231
232 Strain fitness (log2 ratio) -2
-1
0
1
2 at 228.917 kb on - strand at 228.917 kb on - strand at 228.917 kb on - strand at 228.917 kb on - strand at 228.917 kb on - strand at 228.931 kb on - strand at 228.931 kb on - strand at 228.995 kb on - strand at 228.995 kb on - strand at 228.995 kb on - strand at 228.995 kb on - strand at 229.054 kb on - strand at 229.054 kb on - strand at 229.077 kb on - strand at 229.077 kb on - strand at 229.087 kb on + strand at 229.088 kb on - strand at 229.088 kb on - strand at 229.129 kb on + strand at 229.130 kb on - strand at 229.472 kb on - strand at 229.512 kb on + strand at 229.538 kb on + strand at 229.538 kb on + strand at 229.539 kb on - strand at 229.539 kb on - strand at 229.542 kb on + strand at 229.542 kb on + strand at 229.542 kb on + strand at 229.542 kb on + strand at 229.542 kb on + strand at 229.542 kb on + strand at 229.542 kb on + strand at 229.543 kb on - strand at 229.543 kb on - strand at 229.543 kb on - strand at 229.544 kb on + strand at 229.544 kb on + strand at 229.544 kb on + strand at 229.544 kb on + strand at 229.544 kb on + strand at 229.544 kb on + strand at 229.545 kb on - strand at 229.545 kb on - strand at 229.545 kb on - strand at 229.545 kb on - strand at 229.545 kb on - strand at 229.565 kb on - strand at 229.565 kb on - strand at 229.589 kb on + strand at 229.589 kb on + strand at 229.589 kb on + strand at 229.590 kb on - strand at 229.590 kb on - strand at 229.612 kb on + strand at 229.625 kb on + strand at 229.625 kb on + strand at 229.626 kb on - strand at 229.626 kb on - strand at 229.626 kb on - strand at 230.670 kb on + strand, within ppx at 230.826 kb on + strand, within ppx at 230.826 kb on + strand, within ppx at 230.826 kb on + strand, within ppx at 230.827 kb on - strand, within ppx at 230.827 kb on - strand, within ppx at 230.827 kb on - strand, within ppx at 230.827 kb on - strand, within ppx at 230.827 kb on - strand, within ppx at 230.832 kb on + strand, within ppx at 230.833 kb on - strand, within ppx at 230.833 kb on - strand, within ppx at 230.850 kb on + strand, within ppx at 230.850 kb on + strand, within ppx at 230.851 kb on - strand, within ppx at 231.190 kb on + strand at 231.190 kb on + strand at 231.191 kb on - strand at 231.191 kb on - strand at 231.195 kb on + strand at 231.195 kb on + strand at 231.274 kb on + strand at 231.274 kb on + strand at 231.274 kb on + strand at 231.274 kb on + strand at 231.274 kb on + strand at 231.274 kb on + strand at 231.275 kb on - strand at 231.275 kb on - strand at 231.275 kb on - strand at 231.275 kb on - strand at 231.279 kb on - strand at 231.279 kb on - strand at 231.326 kb on + strand at 231.326 kb on + strand at 231.327 kb on - strand at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.467 kb on + strand, within ppk1 at 231.468 kb on - strand, within ppk1 at 231.468 kb on - strand, within ppk1 at 231.468 kb on - strand, within ppk1 at 231.468 kb on - strand, within ppk1 at 231.468 kb on - strand, within ppk1 at 231.582 kb on + strand, within ppk1 at 231.608 kb on + strand, within ppk1 at 231.608 kb on + strand, within ppk1 at 231.608 kb on + strand, within ppk1 at 231.608 kb on + strand, within ppk1 at 231.609 kb on - strand, within ppk1 at 231.609 kb on - strand, within ppk1 at 231.609 kb on - strand, within ppk1 at 231.609 kb on - strand, within ppk1 at 231.609 kb on - strand, within ppk1 at 231.845 kb on + strand, within ppk1 at 231.846 kb on - strand, within ppk1 at 231.846 kb on - strand, within ppk1 at 231.854 kb on + strand, within ppk1 at 231.854 kb on + strand, within ppk1 at 231.855 kb on - strand, within ppk1 at 231.878 kb on + strand, within ppk1 at 231.878 kb on + strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.879 kb on - strand, within ppk1 at 231.902 kb on + strand, within ppk1 at 231.903 kb on - strand, within ppk1 at 231.977 kb on + strand, within ppk1 at 231.978 kb on - strand, within ppk1 at 231.978 kb on - strand, within ppk1 at 232.136 kb on + strand, within ppk1
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 228,917 - +0.7 228,917 - -0.0 228,917 - +0.2 228,917 - -0.1 228,917 - -0.1 228,931 - +0.1 228,931 - -0.8 228,995 - -1.4 228,995 - +0.0 228,995 - +1.6 228,995 - +0.8 229,054 - -0.8 229,054 - -0.3 229,077 - -0.4 229,077 - -0.9 229,087 + +0.3 229,088 - +0.3 229,088 - +0.8 229,129 + -0.3 229,130 - -0.1 229,472 - -0.8 229,512 + -0.7 229,538 + -0.9 229,538 + +0.5 229,539 - +0.7 229,539 - -0.3 229,542 + -1.0 229,542 + -0.7 229,542 + +0.2 229,542 + -0.1 229,542 + -1.8 229,542 + -1.0 229,542 + -0.1 229,543 - -0.3 229,543 - -0.8 229,543 - -0.3 229,544 + -0.7 229,544 + -1.3 229,544 + -0.0 229,544 + -0.4 229,544 + +1.3 229,544 + -0.1 229,545 - -0.5 229,545 - -0.1 229,545 - +0.2 229,545 - +0.0 229,545 - -0.5 229,565 - -0.6 229,565 - -1.6 229,589 + -0.7 229,589 + +0.4 229,589 + +1.3 229,590 - -0.5 229,590 - -0.3 229,612 + +0.2 229,625 + -1.0 229,625 + -0.0 229,626 - +0.6 229,626 - -0.7 229,626 - -0.3 230,670 + ppx COO64_RS01075 0.65 -0.8 230,826 + ppx COO64_RS01075 0.75 +1.6 230,826 + ppx COO64_RS01075 0.75 -0.7 230,826 + ppx COO64_RS01075 0.75 +0.3 230,827 - ppx COO64_RS01075 0.75 -0.6 230,827 - ppx COO64_RS01075 0.75 -1.1 230,827 - ppx COO64_RS01075 0.75 -0.3 230,827 - ppx COO64_RS01075 0.75 +2.0 230,827 - ppx COO64_RS01075 0.75 -1.0 230,832 + ppx COO64_RS01075 0.76 +0.3 230,833 - ppx COO64_RS01075 0.76 -1.4 230,833 - ppx COO64_RS01075 0.76 +0.7 230,850 + ppx COO64_RS01075 0.77 -0.8 230,850 + ppx COO64_RS01075 0.77 +0.8 230,851 - ppx COO64_RS01075 0.77 -0.3 231,190 + -0.2 231,190 + +0.0 231,191 - +1.2 231,191 - +0.7 231,195 + -0.5 231,195 + +0.1 231,274 + +0.1 231,274 + +0.2 231,274 + +0.8 231,274 + -0.5 231,274 + -1.0 231,274 + +0.6 231,275 - -0.5 231,275 - -0.3 231,275 - -0.4 231,275 - -0.7 231,279 - +0.6 231,279 - +0.4 231,326 + +0.4 231,326 + +0.6 231,327 - +0.8 231,467 + ppk1 COO64_RS01080 0.13 -1.6 231,467 + ppk1 COO64_RS01080 0.13 -0.1 231,467 + ppk1 COO64_RS01080 0.13 -0.1 231,467 + ppk1 COO64_RS01080 0.13 +0.0 231,467 + ppk1 COO64_RS01080 0.13 +0.5 231,467 + ppk1 COO64_RS01080 0.13 +1.6 231,467 + ppk1 COO64_RS01080 0.13 +0.6 231,467 + ppk1 COO64_RS01080 0.13 +0.1 231,467 + ppk1 COO64_RS01080 0.13 -0.7 231,468 - ppk1 COO64_RS01080 0.13 -2.0 231,468 - ppk1 COO64_RS01080 0.13 -0.3 231,468 - ppk1 COO64_RS01080 0.13 +1.0 231,468 - ppk1 COO64_RS01080 0.13 +0.5 231,468 - ppk1 COO64_RS01080 0.13 +0.3 231,582 + ppk1 COO64_RS01080 0.18 +0.1 231,608 + ppk1 COO64_RS01080 0.19 +0.2 231,608 + ppk1 COO64_RS01080 0.19 +0.4 231,608 + ppk1 COO64_RS01080 0.19 -0.5 231,608 + ppk1 COO64_RS01080 0.19 +0.0 231,609 - ppk1 COO64_RS01080 0.19 -0.2 231,609 - ppk1 COO64_RS01080 0.19 -0.9 231,609 - ppk1 COO64_RS01080 0.19 +0.3 231,609 - ppk1 COO64_RS01080 0.19 +0.4 231,609 - ppk1 COO64_RS01080 0.19 +0.1 231,845 + ppk1 COO64_RS01080 0.30 -0.1 231,846 - ppk1 COO64_RS01080 0.30 +0.6 231,846 - ppk1 COO64_RS01080 0.30 +0.0 231,854 + ppk1 COO64_RS01080 0.30 +1.5 231,854 + ppk1 COO64_RS01080 0.30 +1.0 231,855 - ppk1 COO64_RS01080 0.30 -0.7 231,878 + ppk1 COO64_RS01080 0.31 +0.4 231,878 + ppk1 COO64_RS01080 0.31 -0.7 231,879 - ppk1 COO64_RS01080 0.31 -0.1 231,879 - ppk1 COO64_RS01080 0.31 +1.0 231,879 - ppk1 COO64_RS01080 0.31 +0.6 231,879 - ppk1 COO64_RS01080 0.31 +1.1 231,879 - ppk1 COO64_RS01080 0.31 +1.3 231,879 - ppk1 COO64_RS01080 0.31 -0.2 231,879 - ppk1 COO64_RS01080 0.31 -0.4 231,879 - ppk1 COO64_RS01080 0.31 -0.5 231,879 - ppk1 COO64_RS01080 0.31 -0.1 231,879 - ppk1 COO64_RS01080 0.31 -1.8 231,902 + ppk1 COO64_RS01080 0.32 +0.4 231,903 - ppk1 COO64_RS01080 0.32 +0.3 231,977 + ppk1 COO64_RS01080 0.36 -0.2 231,978 - ppk1 COO64_RS01080 0.36 -0.5 231,978 - ppk1 COO64_RS01080 0.36 +0.8 232,136 + ppk1 COO64_RS01080 0.43 +0.5
Or see this region's nucleotide sequence