Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and COO64_RS21320 are separated by 76 nucleotides COO64_RS21320 and COO64_RS21325 are separated by 71 nucleotides COO64_RS21325 and COO64_RS21330 are separated by 445 nucleotides
COO64_RS21315: fliD - flagellar filament capping protein FliD, at 20,743 to 22,149
fliD
COO64_RS21320: COO64_RS21320 - flagellar protein FlaG, at 22,226 to 22,591
_RS21320
COO64_RS21325: COO64_RS21325 - flagellin domain-containing protein, at 22,663 to 23,511
_RS21325
COO64_RS21330: COO64_RS21330 - ketoacyl-ACP synthase III, at 23,957 to 24,886
_RS21330
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 21.848 kb on + strand, within fliD at 21.849 kb on - strand, within fliD at 21.849 kb on - strand, within fliD at 21.849 kb on - strand, within fliD at 21.868 kb on - strand, within fliD at 21.868 kb on - strand, within fliD at 21.917 kb on + strand, within fliD at 21.917 kb on + strand, within fliD at 21.917 kb on + strand, within fliD at 21.918 kb on - strand, within fliD at 21.971 kb on + strand, within fliD at 22.105 kb on + strand at 22.106 kb on - strand at 22.141 kb on - strand at 22.149 kb on + strand at 22.149 kb on + strand at 22.149 kb on + strand at 22.150 kb on - strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.153 kb on + strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.154 kb on - strand at 22.167 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.256 kb on + strand at 22.257 kb on - strand at 22.257 kb on - strand at 22.342 kb on + strand, within COO64_RS21320 at 22.342 kb on + strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.343 kb on - strand, within COO64_RS21320 at 22.346 kb on + strand, within COO64_RS21320 at 22.346 kb on + strand, within COO64_RS21320 at 22.347 kb on - strand, within COO64_RS21320 at 22.347 kb on - strand, within COO64_RS21320 at 22.347 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.388 kb on - strand, within COO64_RS21320 at 22.561 kb on - strand at 22.585 kb on + strand at 22.585 kb on + strand at 22.586 kb on - strand at 22.629 kb on + strand at 22.630 kb on - strand at 22.630 kb on - strand at 22.630 kb on - strand at 22.656 kb on + strand at 22.656 kb on + strand at 22.782 kb on + strand, within COO64_RS21325 at 22.782 kb on + strand, within COO64_RS21325 at 22.783 kb on - strand, within COO64_RS21325 at 22.783 kb on - strand, within COO64_RS21325 at 22.789 kb on + strand, within COO64_RS21325 at 22.789 kb on + strand, within COO64_RS21325 at 22.798 kb on - strand, within COO64_RS21325 at 22.854 kb on + strand, within COO64_RS21325 at 22.854 kb on + strand, within COO64_RS21325 at 22.855 kb on - strand, within COO64_RS21325 at 22.855 kb on - strand, within COO64_RS21325 at 22.855 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.865 kb on - strand, within COO64_RS21325 at 22.905 kb on + strand, within COO64_RS21325 at 23.017 kb on - strand, within COO64_RS21325 at 23.017 kb on - strand, within COO64_RS21325 at 23.184 kb on + strand, within COO64_RS21325 at 23.184 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.238 kb on + strand, within COO64_RS21325 at 23.239 kb on - strand, within COO64_RS21325 at 23.298 kb on + strand, within COO64_RS21325 at 23.299 kb on - strand, within COO64_RS21325 at 23.299 kb on - strand, within COO64_RS21325 at 23.299 kb on - strand, within COO64_RS21325 at 23.353 kb on - strand, within COO64_RS21325 at 23.353 kb on - strand, within COO64_RS21325 at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.484 kb on + strand at 23.485 kb on - strand at 23.485 kb on - strand at 23.490 kb on + strand at 23.490 kb on + strand at 23.490 kb on + strand at 23.490 kb on + strand at 23.535 kb on - strand at 23.573 kb on + strand at 23.574 kb on - strand at 23.574 kb on - strand at 23.574 kb on - strand at 23.586 kb on + strand at 23.586 kb on + strand at 23.587 kb on - strand at 23.587 kb on - strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.589 kb on + strand at 23.590 kb on - strand at 23.590 kb on - strand at 23.590 kb on - strand at 23.590 kb on - strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.593 kb on + strand at 23.594 kb on - strand at 23.594 kb on - strand at 23.594 kb on - strand at 23.594 kb on - strand at 23.693 kb on + strand at 23.693 kb on + strand at 23.745 kb on + strand at 23.745 kb on + strand at 23.835 kb on + strand at 23.835 kb on + strand at 23.835 kb on + strand at 23.836 kb on - strand at 23.836 kb on - strand at 23.888 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.953 kb on + strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.954 kb on - strand at 23.955 kb on + strand at 23.955 kb on + strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 23.956 kb on - strand at 24.085 kb on + strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.086 kb on - strand, within COO64_RS21330 at 24.088 kb on + strand, within COO64_RS21330 at 24.457 kb on - strand, within COO64_RS21330 at 24.457 kb on - strand, within COO64_RS21330 at 24.457 kb on - strand, within COO64_RS21330
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 21,848 + fliD COO64_RS21315 0.79 +1.3 21,849 - fliD COO64_RS21315 0.79 +1.5 21,849 - fliD COO64_RS21315 0.79 -0.2 21,849 - fliD COO64_RS21315 0.79 +1.2 21,868 - fliD COO64_RS21315 0.80 +1.1 21,868 - fliD COO64_RS21315 0.80 +0.5 21,917 + fliD COO64_RS21315 0.83 +0.9 21,917 + fliD COO64_RS21315 0.83 +1.7 21,917 + fliD COO64_RS21315 0.83 +1.7 21,918 - fliD COO64_RS21315 0.84 +0.8 21,971 + fliD COO64_RS21315 0.87 +0.5 22,105 + +0.7 22,106 - +2.2 22,141 - +0.9 22,149 + +1.7 22,149 + +0.5 22,149 + +0.2 22,150 - +2.4 22,153 + +1.9 22,153 + +1.9 22,153 + +2.0 22,153 + +1.4 22,153 + +0.4 22,154 - +0.4 22,154 - +0.6 22,154 - -0.5 22,154 - +0.3 22,154 - +0.4 22,154 - -0.8 22,167 + +1.0 22,256 + +0.0 22,256 + +0.7 22,256 + +0.6 22,256 + +1.0 22,256 + +0.7 22,256 + -0.5 22,257 - -0.4 22,257 - -0.1 22,342 + COO64_RS21320 0.32 +1.4 22,342 + COO64_RS21320 0.32 -0.5 22,343 - COO64_RS21320 0.32 +0.5 22,343 - COO64_RS21320 0.32 -1.1 22,343 - COO64_RS21320 0.32 +0.6 22,343 - COO64_RS21320 0.32 +0.5 22,343 - COO64_RS21320 0.32 -0.6 22,346 + COO64_RS21320 0.33 -0.7 22,346 + COO64_RS21320 0.33 -0.9 22,347 - COO64_RS21320 0.33 -0.5 22,347 - COO64_RS21320 0.33 +0.9 22,347 - COO64_RS21320 0.33 +2.0 22,388 - COO64_RS21320 0.44 +0.9 22,388 - COO64_RS21320 0.44 -0.2 22,388 - COO64_RS21320 0.44 -0.7 22,388 - COO64_RS21320 0.44 +1.7 22,561 - +0.8 22,585 + +0.7 22,585 + -0.1 22,586 - -0.5 22,629 + +1.8 22,630 - +0.2 22,630 - -0.2 22,630 - +1.3 22,656 + +1.8 22,656 + +1.0 22,782 + COO64_RS21325 0.14 +1.4 22,782 + COO64_RS21325 0.14 +1.4 22,783 - COO64_RS21325 0.14 +0.7 22,783 - COO64_RS21325 0.14 +1.2 22,789 + COO64_RS21325 0.15 +2.3 22,789 + COO64_RS21325 0.15 +2.7 22,798 - COO64_RS21325 0.16 +0.1 22,854 + COO64_RS21325 0.22 +2.0 22,854 + COO64_RS21325 0.22 +1.9 22,855 - COO64_RS21325 0.23 +0.7 22,855 - COO64_RS21325 0.23 +1.4 22,855 - COO64_RS21325 0.23 +1.8 22,865 - COO64_RS21325 0.24 +0.3 22,865 - COO64_RS21325 0.24 +1.9 22,865 - COO64_RS21325 0.24 +1.6 22,865 - COO64_RS21325 0.24 +1.7 22,905 + COO64_RS21325 0.29 +0.8 23,017 - COO64_RS21325 0.42 +0.6 23,017 - COO64_RS21325 0.42 -0.3 23,184 + COO64_RS21325 0.61 -0.5 23,184 + COO64_RS21325 0.61 +1.0 23,238 + COO64_RS21325 0.68 -1.0 23,238 + COO64_RS21325 0.68 +0.6 23,238 + COO64_RS21325 0.68 +0.6 23,238 + COO64_RS21325 0.68 +1.9 23,239 - COO64_RS21325 0.68 +1.6 23,298 + COO64_RS21325 0.75 +2.7 23,299 - COO64_RS21325 0.75 +2.7 23,299 - COO64_RS21325 0.75 +2.0 23,299 - COO64_RS21325 0.75 -0.4 23,353 - COO64_RS21325 0.81 +0.6 23,353 - COO64_RS21325 0.81 +1.2 23,484 + +0.8 23,484 + +0.6 23,484 + +0.3 23,484 + +1.1 23,484 + +0.9 23,484 + +0.9 23,484 + +1.0 23,484 + +0.2 23,484 + +0.4 23,484 + +1.3 23,484 + +1.0 23,485 - +1.0 23,485 - +0.8 23,490 + +1.1 23,490 + +0.1 23,490 + +0.4 23,490 + +0.1 23,535 - -0.9 23,573 + -0.5 23,574 - +1.1 23,574 - +0.7 23,574 - +1.1 23,586 + +1.1 23,586 + +0.1 23,587 - -0.2 23,587 - +0.7 23,589 + +1.5 23,589 + +1.2 23,589 + +0.2 23,589 + +1.7 23,589 + +1.5 23,590 - +1.7 23,590 - +0.7 23,590 - +1.6 23,590 - +0.1 23,593 + +1.1 23,593 + +1.0 23,593 + +1.1 23,593 + +0.9 23,593 + +0.8 23,593 + +1.3 23,593 + +1.3 23,594 - +0.2 23,594 - +2.0 23,594 - +1.4 23,594 - +0.3 23,693 + +0.3 23,693 + -0.0 23,745 + -1.9 23,745 + -0.2 23,835 + +0.4 23,835 + -0.7 23,835 + -0.4 23,836 - -0.3 23,836 - +0.6 23,888 + -0.4 23,953 + +0.4 23,953 + -0.1 23,953 + -0.6 23,953 + -0.7 23,953 + -0.9 23,953 + -0.6 23,954 - -0.8 23,954 - -0.4 23,954 - -0.3 23,954 - -0.2 23,954 - -0.0 23,954 - -0.4 23,955 + -1.9 23,955 + -0.8 23,956 - -0.2 23,956 - -2.0 23,956 - -1.3 23,956 - +0.5 23,956 - -0.4 23,956 - -0.1 24,085 + COO64_RS21330 0.14 +0.5 24,086 - COO64_RS21330 0.14 -0.5 24,086 - COO64_RS21330 0.14 -0.5 24,086 - COO64_RS21330 0.14 -0.1 24,086 - COO64_RS21330 0.14 -1.0 24,086 - COO64_RS21330 0.14 -0.3 24,088 + COO64_RS21330 0.14 -0.0 24,457 - COO64_RS21330 0.54 -0.5 24,457 - COO64_RS21330 0.54 -0.8 24,457 - COO64_RS21330 0.54 -1.0
Or see this region's nucleotide sequence