Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tig and clpP are separated by 91 nucleotides clpP and clpX are separated by 108 nucleotides clpX and lon are separated by 151 nucleotides
COO64_RS19190: tig - trigger factor, at 157,457 to 158,767
tig
COO64_RS19195: clpP - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 158,859 to 159,500
clpP
COO64_RS19200: clpX - ATP-dependent Clp protease ATP-binding subunit ClpX, at 159,609 to 160,892
clpX
COO64_RS19205: lon - endopeptidase La, at 161,044 to 163,440
lon
Position (kb) 159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 158.615 kb on + strand, within tig at 158.615 kb on + strand, within tig at 158.616 kb on - strand, within tig at 158.618 kb on + strand, within tig at 158.619 kb on - strand, within tig at 158.619 kb on - strand, within tig at 158.619 kb on - strand, within tig at 158.724 kb on - strand at 158.724 kb on - strand at 158.823 kb on - strand at 158.837 kb on + strand at 158.837 kb on + strand at 158.837 kb on + strand at 158.837 kb on + strand at 158.838 kb on - strand at 158.838 kb on - strand at 158.838 kb on - strand at 158.838 kb on - strand at 158.838 kb on - strand at 158.874 kb on + strand at 158.875 kb on - strand at 158.878 kb on - strand at 158.958 kb on + strand, within clpP at 158.958 kb on + strand, within clpP at 158.959 kb on - strand, within clpP at 158.967 kb on + strand, within clpP at 158.967 kb on + strand, within clpP at 158.968 kb on - strand, within clpP at 158.968 kb on - strand, within clpP at 158.968 kb on - strand, within clpP at 158.968 kb on - strand, within clpP at 159.021 kb on + strand, within clpP at 159.021 kb on + strand, within clpP at 159.021 kb on + strand, within clpP at 159.022 kb on - strand, within clpP at 159.022 kb on - strand, within clpP at 159.022 kb on - strand, within clpP at 159.093 kb on + strand, within clpP at 159.094 kb on - strand, within clpP at 159.094 kb on - strand, within clpP at 159.094 kb on - strand, within clpP at 159.129 kb on - strand, within clpP at 159.138 kb on + strand, within clpP at 159.139 kb on - strand, within clpP at 159.373 kb on - strand, within clpP at 159.474 kb on + strand at 159.537 kb on + strand at 159.538 kb on - strand at 159.651 kb on + strand at 159.651 kb on + strand at 159.652 kb on - strand at 159.652 kb on - strand at 159.652 kb on - strand at 159.652 kb on - strand at 159.712 kb on + strand at 159.712 kb on + strand at 159.712 kb on + strand at 159.716 kb on + strand at 159.716 kb on + strand at 159.717 kb on - strand at 159.840 kb on + strand, within clpX at 159.840 kb on + strand, within clpX at 159.841 kb on - strand, within clpX at 159.841 kb on - strand, within clpX at 159.841 kb on - strand, within clpX at 159.841 kb on - strand, within clpX at 159.888 kb on + strand, within clpX at 159.888 kb on + strand, within clpX at 159.888 kb on + strand, within clpX at 159.889 kb on - strand, within clpX at 159.889 kb on - strand, within clpX at 159.897 kb on + strand, within clpX at 159.897 kb on + strand, within clpX at 159.897 kb on + strand, within clpX at 159.898 kb on - strand, within clpX at 159.953 kb on + strand, within clpX at 159.954 kb on - strand, within clpX at 159.954 kb on - strand, within clpX at 159.954 kb on - strand, within clpX at 160.027 kb on + strand, within clpX at 160.027 kb on + strand, within clpX at 160.028 kb on - strand, within clpX at 160.125 kb on + strand, within clpX at 160.126 kb on - strand, within clpX at 160.126 kb on - strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.158 kb on + strand, within clpX at 160.159 kb on - strand, within clpX at 160.159 kb on - strand, within clpX at 160.159 kb on - strand, within clpX at 160.337 kb on + strand, within clpX at 160.432 kb on + strand, within clpX at 160.432 kb on + strand, within clpX at 160.432 kb on + strand, within clpX at 160.432 kb on + strand, within clpX at 160.621 kb on - strand, within clpX at 160.764 kb on + strand at 160.764 kb on + strand at 160.764 kb on + strand at 160.764 kb on + strand at 160.765 kb on - strand at 160.765 kb on - strand at 160.765 kb on - strand at 160.765 kb on - strand at 160.849 kb on - strand at 160.985 kb on + strand at 160.985 kb on + strand at 160.985 kb on + strand at 160.986 kb on - strand at 160.986 kb on - strand at 160.986 kb on - strand at 161.020 kb on + strand at 161.031 kb on + strand at 161.032 kb on - strand at 161.032 kb on - strand at 161.032 kb on - strand at 161.103 kb on + strand at 161.103 kb on + strand at 161.247 kb on + strand at 161.427 kb on + strand, within lon at 161.428 kb on - strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.452 kb on + strand, within lon at 161.453 kb on - strand, within lon at 161.453 kb on - strand, within lon at 161.453 kb on - strand, within lon at 161.453 kb on - strand, within lon at 161.453 kb on - strand, within lon at 161.453 kb on - strand, within lon at 161.713 kb on + strand, within lon at 161.714 kb on - strand, within lon at 161.716 kb on + strand, within lon at 161.716 kb on + strand, within lon at 161.717 kb on - strand, within lon at 161.717 kb on - strand, within lon at 161.717 kb on - strand, within lon at 161.717 kb on - strand, within lon at 161.833 kb on + strand, within lon at 161.833 kb on + strand, within lon at 161.833 kb on + strand, within lon
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 158,615 + tig COO64_RS19190 0.88 -0.7 158,615 + tig COO64_RS19190 0.88 -0.8 158,616 - tig COO64_RS19190 0.88 -0.9 158,618 + tig COO64_RS19190 0.89 -0.7 158,619 - tig COO64_RS19190 0.89 +0.1 158,619 - tig COO64_RS19190 0.89 -1.2 158,619 - tig COO64_RS19190 0.89 -0.5 158,724 - +0.7 158,724 - +0.7 158,823 - -0.3 158,837 + +1.3 158,837 + -0.3 158,837 + +0.0 158,837 + +0.0 158,838 - -0.0 158,838 - +0.3 158,838 - -0.7 158,838 - -0.3 158,838 - +0.3 158,874 + -0.9 158,875 - +1.3 158,878 - +3.3 158,958 + clpP COO64_RS19195 0.15 +1.3 158,958 + clpP COO64_RS19195 0.15 -0.5 158,959 - clpP COO64_RS19195 0.16 -0.0 158,967 + clpP COO64_RS19195 0.17 -0.3 158,967 + clpP COO64_RS19195 0.17 +0.7 158,968 - clpP COO64_RS19195 0.17 -0.0 158,968 - clpP COO64_RS19195 0.17 +1.3 158,968 - clpP COO64_RS19195 0.17 +0.3 158,968 - clpP COO64_RS19195 0.17 -0.3 159,021 + clpP COO64_RS19195 0.25 -0.0 159,021 + clpP COO64_RS19195 0.25 -0.5 159,021 + clpP COO64_RS19195 0.25 -0.0 159,022 - clpP COO64_RS19195 0.25 -0.3 159,022 - clpP COO64_RS19195 0.25 -1.0 159,022 - clpP COO64_RS19195 0.25 +1.7 159,093 + clpP COO64_RS19195 0.36 -0.0 159,094 - clpP COO64_RS19195 0.37 -1.5 159,094 - clpP COO64_RS19195 0.37 -0.7 159,094 - clpP COO64_RS19195 0.37 -0.0 159,129 - clpP COO64_RS19195 0.42 -0.5 159,138 + clpP COO64_RS19195 0.43 -0.0 159,139 - clpP COO64_RS19195 0.44 -0.5 159,373 - clpP COO64_RS19195 0.80 +0.3 159,474 + +0.3 159,537 + +0.7 159,538 - +1.0 159,651 + -0.3 159,651 + -0.5 159,652 - +1.1 159,652 - -1.3 159,652 - -0.7 159,652 - +0.9 159,712 + +0.3 159,712 + +1.5 159,712 + -1.4 159,716 + +0.4 159,716 + -0.3 159,717 - +0.8 159,840 + clpX COO64_RS19200 0.18 -0.7 159,840 + clpX COO64_RS19200 0.18 +0.7 159,841 - clpX COO64_RS19200 0.18 -2.1 159,841 - clpX COO64_RS19200 0.18 -0.2 159,841 - clpX COO64_RS19200 0.18 +0.1 159,841 - clpX COO64_RS19200 0.18 -1.5 159,888 + clpX COO64_RS19200 0.22 -0.6 159,888 + clpX COO64_RS19200 0.22 +0.5 159,888 + clpX COO64_RS19200 0.22 +0.7 159,889 - clpX COO64_RS19200 0.22 -0.5 159,889 - clpX COO64_RS19200 0.22 -0.3 159,897 + clpX COO64_RS19200 0.22 -0.8 159,897 + clpX COO64_RS19200 0.22 +1.3 159,897 + clpX COO64_RS19200 0.22 -1.2 159,898 - clpX COO64_RS19200 0.23 -1.0 159,953 + clpX COO64_RS19200 0.27 -0.5 159,954 - clpX COO64_RS19200 0.27 -0.5 159,954 - clpX COO64_RS19200 0.27 +1.0 159,954 - clpX COO64_RS19200 0.27 -1.0 160,027 + clpX COO64_RS19200 0.33 +0.7 160,027 + clpX COO64_RS19200 0.33 -1.4 160,028 - clpX COO64_RS19200 0.33 +0.2 160,125 + clpX COO64_RS19200 0.40 -1.3 160,126 - clpX COO64_RS19200 0.40 -1.0 160,126 - clpX COO64_RS19200 0.40 +0.7 160,158 + clpX COO64_RS19200 0.43 +0.8 160,158 + clpX COO64_RS19200 0.43 -0.4 160,158 + clpX COO64_RS19200 0.43 +0.3 160,158 + clpX COO64_RS19200 0.43 +0.5 160,158 + clpX COO64_RS19200 0.43 -1.8 160,158 + clpX COO64_RS19200 0.43 -0.3 160,158 + clpX COO64_RS19200 0.43 -0.3 160,158 + clpX COO64_RS19200 0.43 +0.3 160,159 - clpX COO64_RS19200 0.43 -1.3 160,159 - clpX COO64_RS19200 0.43 +0.1 160,159 - clpX COO64_RS19200 0.43 -0.2 160,337 + clpX COO64_RS19200 0.57 -0.0 160,432 + clpX COO64_RS19200 0.64 +1.7 160,432 + clpX COO64_RS19200 0.64 -2.0 160,432 + clpX COO64_RS19200 0.64 -0.7 160,432 + clpX COO64_RS19200 0.64 +1.9 160,621 - clpX COO64_RS19200 0.79 -0.4 160,764 + -0.5 160,764 + -1.3 160,764 + -0.0 160,764 + -1.5 160,765 - +0.7 160,765 - -0.3 160,765 - -1.2 160,765 - +0.3 160,849 - -1.0 160,985 + +0.2 160,985 + +0.3 160,985 + -1.3 160,986 - +0.4 160,986 - +1.3 160,986 - -0.2 161,020 + -0.7 161,031 + -0.6 161,032 - -0.5 161,032 - -1.5 161,032 - -0.7 161,103 + +0.8 161,103 + -0.3 161,247 + -1.1 161,427 + lon COO64_RS19205 0.16 -2.8 161,428 - lon COO64_RS19205 0.16 -0.9 161,452 + lon COO64_RS19205 0.17 -0.5 161,452 + lon COO64_RS19205 0.17 -0.3 161,452 + lon COO64_RS19205 0.17 -0.0 161,452 + lon COO64_RS19205 0.17 +0.4 161,452 + lon COO64_RS19205 0.17 +0.1 161,452 + lon COO64_RS19205 0.17 +0.1 161,452 + lon COO64_RS19205 0.17 -0.6 161,452 + lon COO64_RS19205 0.17 +0.6 161,452 + lon COO64_RS19205 0.17 -0.5 161,453 - lon COO64_RS19205 0.17 +0.2 161,453 - lon COO64_RS19205 0.17 -0.8 161,453 - lon COO64_RS19205 0.17 -2.2 161,453 - lon COO64_RS19205 0.17 -2.6 161,453 - lon COO64_RS19205 0.17 +0.3 161,453 - lon COO64_RS19205 0.17 +0.6 161,713 + lon COO64_RS19205 0.28 -0.2 161,714 - lon COO64_RS19205 0.28 -0.2 161,716 + lon COO64_RS19205 0.28 -0.4 161,716 + lon COO64_RS19205 0.28 -0.3 161,717 - lon COO64_RS19205 0.28 -0.0 161,717 - lon COO64_RS19205 0.28 -1.7 161,717 - lon COO64_RS19205 0.28 +0.2 161,717 - lon COO64_RS19205 0.28 -1.0 161,833 + lon COO64_RS19205 0.33 -1.0 161,833 + lon COO64_RS19205 0.33 -0.3 161,833 + lon COO64_RS19205 0.33 -0.5
Or see this region's nucleotide sequence