Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS14335 and cobF are separated by 72 nucleotides cobF and COO64_RS14345 overlap by 35 nucleotides COO64_RS14345 and COO64_RS14350 are separated by 52 nucleotides COO64_RS14350 and COO64_RS14355 are separated by 296 nucleotides
COO64_RS14335: COO64_RS14335 - OprD family porin, at 428,000 to 429,289
_RS14335
COO64_RS14340: cobF - precorrin-6A synthase (deacetylating), at 429,362 to 430,111
cobF
COO64_RS14345: COO64_RS14345 - glycosyltransferase, at 430,077 to 431,396
_RS14345
COO64_RS14350: COO64_RS14350 - response regulator, at 431,449 to 431,820
_RS14350
COO64_RS14355: COO64_RS14355 - response regulator, at 432,117 to 435,599
_RS14355
Position (kb) 430
431
432 Strain fitness (log2 ratio) -2
-1
0
1 at 429.091 kb on + strand, within COO64_RS14335 at 429.092 kb on - strand, within COO64_RS14335 at 429.092 kb on - strand, within COO64_RS14335 at 429.092 kb on - strand, within COO64_RS14335 at 429.152 kb on + strand, within COO64_RS14335 at 429.152 kb on + strand, within COO64_RS14335 at 429.153 kb on - strand, within COO64_RS14335 at 429.199 kb on + strand at 429.200 kb on - strand at 429.209 kb on + strand at 429.209 kb on + strand at 429.221 kb on + strand at 429.222 kb on - strand at 429.222 kb on - strand at 429.230 kb on + strand at 429.230 kb on + strand at 429.231 kb on - strand at 429.231 kb on - strand at 429.231 kb on - strand at 429.269 kb on + strand at 429.269 kb on + strand at 429.269 kb on + strand at 429.269 kb on + strand at 429.270 kb on - strand at 429.297 kb on + strand at 429.297 kb on + strand at 429.297 kb on + strand at 429.297 kb on + strand at 429.297 kb on + strand at 429.297 kb on + strand at 429.297 kb on + strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.298 kb on - strand at 429.327 kb on + strand at 429.327 kb on + strand at 429.328 kb on - strand at 429.328 kb on - strand at 429.329 kb on + strand at 429.329 kb on + strand at 429.329 kb on + strand at 429.330 kb on - strand at 429.590 kb on + strand, within cobF at 429.591 kb on - strand, within cobF at 429.591 kb on - strand, within cobF at 429.635 kb on + strand, within cobF at 429.635 kb on + strand, within cobF at 429.635 kb on + strand, within cobF at 429.636 kb on - strand, within cobF at 429.636 kb on - strand, within cobF at 429.636 kb on - strand, within cobF at 429.711 kb on - strand, within cobF at 429.711 kb on - strand, within cobF at 429.742 kb on - strand, within cobF at 429.836 kb on - strand, within cobF at 429.962 kb on + strand, within cobF at 429.962 kb on + strand, within cobF at 429.962 kb on + strand, within cobF at 429.962 kb on + strand, within cobF at 429.962 kb on + strand, within cobF at 429.963 kb on - strand, within cobF at 429.974 kb on + strand, within cobF at 430.088 kb on + strand at 430.088 kb on + strand at 430.088 kb on + strand at 430.088 kb on + strand at 430.088 kb on + strand at 430.088 kb on + strand at 430.089 kb on - strand at 430.089 kb on - strand at 430.089 kb on - strand at 430.089 kb on - strand at 430.089 kb on - strand at 430.089 kb on - strand at 430.147 kb on + strand at 430.148 kb on - strand at 430.148 kb on - strand at 430.340 kb on - strand, within COO64_RS14345 at 430.340 kb on - strand, within COO64_RS14345 at 430.473 kb on - strand, within COO64_RS14345 at 430.589 kb on + strand, within COO64_RS14345 at 430.590 kb on - strand, within COO64_RS14345 at 430.618 kb on + strand, within COO64_RS14345 at 430.618 kb on + strand, within COO64_RS14345 at 430.618 kb on + strand, within COO64_RS14345 at 430.618 kb on + strand, within COO64_RS14345 at 430.619 kb on - strand, within COO64_RS14345 at 430.619 kb on - strand, within COO64_RS14345 at 430.627 kb on + strand, within COO64_RS14345 at 430.627 kb on + strand, within COO64_RS14345 at 430.628 kb on - strand, within COO64_RS14345 at 430.628 kb on - strand, within COO64_RS14345 at 430.628 kb on - strand, within COO64_RS14345 at 430.628 kb on - strand, within COO64_RS14345 at 430.631 kb on + strand, within COO64_RS14345 at 430.631 kb on + strand, within COO64_RS14345 at 430.631 kb on + strand, within COO64_RS14345 at 430.631 kb on + strand, within COO64_RS14345 at 430.632 kb on - strand, within COO64_RS14345 at 430.632 kb on - strand, within COO64_RS14345 at 430.632 kb on - strand, within COO64_RS14345 at 430.632 kb on - strand, within COO64_RS14345 at 430.632 kb on - strand, within COO64_RS14345 at 430.891 kb on + strand, within COO64_RS14345 at 430.891 kb on + strand, within COO64_RS14345 at 430.891 kb on + strand, within COO64_RS14345 at 430.892 kb on - strand, within COO64_RS14345 at 430.892 kb on - strand, within COO64_RS14345 at 431.273 kb on + strand at 431.274 kb on - strand at 431.359 kb on + strand at 431.359 kb on + strand at 431.359 kb on + strand at 431.360 kb on - strand at 431.360 kb on - strand at 431.360 kb on - strand at 431.364 kb on + strand at 431.364 kb on + strand at 431.364 kb on + strand at 431.365 kb on - strand at 431.390 kb on + strand at 431.390 kb on + strand at 431.426 kb on - strand at 431.428 kb on + strand at 431.429 kb on - strand at 431.450 kb on + strand at 431.450 kb on + strand at 431.450 kb on + strand at 431.451 kb on - strand at 431.451 kb on - strand at 431.623 kb on - strand, within COO64_RS14350 at 431.852 kb on + strand at 431.852 kb on + strand at 431.853 kb on - strand at 431.853 kb on - strand at 431.853 kb on - strand at 431.853 kb on - strand at 431.853 kb on - strand at 431.853 kb on - strand at 431.886 kb on + strand at 431.895 kb on + strand at 431.918 kb on + strand at 431.918 kb on + strand at 431.927 kb on - strand at 431.956 kb on + strand at 431.956 kb on + strand at 432.006 kb on - strand at 432.146 kb on + strand at 432.146 kb on + strand at 432.147 kb on - strand at 432.147 kb on - strand at 432.156 kb on - strand at 432.234 kb on + strand at 432.234 kb on + strand at 432.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 429,091 + COO64_RS14335 0.85 +0.4 429,092 - COO64_RS14335 0.85 -0.0 429,092 - COO64_RS14335 0.85 +1.1 429,092 - COO64_RS14335 0.85 -0.7 429,152 + COO64_RS14335 0.89 -0.7 429,152 + COO64_RS14335 0.89 +1.1 429,153 - COO64_RS14335 0.89 +0.3 429,199 + -0.5 429,200 - +0.4 429,209 + +0.8 429,209 + -0.0 429,221 + -2.2 429,222 - -0.4 429,222 - -0.9 429,230 + +0.1 429,230 + +0.3 429,231 - +1.7 429,231 - -2.2 429,231 - +0.4 429,269 + -0.7 429,269 + -1.0 429,269 + +1.0 429,269 + -0.4 429,270 - -0.8 429,297 + +0.1 429,297 + +0.4 429,297 + +0.1 429,297 + +0.1 429,297 + +0.0 429,297 + +0.2 429,297 + -0.2 429,298 - -0.6 429,298 - +0.1 429,298 - -1.9 429,298 - +0.4 429,298 - -0.3 429,298 - -0.2 429,298 - -0.3 429,298 - +0.3 429,327 + -0.5 429,327 + +0.2 429,328 - -0.2 429,328 - -0.6 429,329 + -0.2 429,329 + -0.3 429,329 + +0.4 429,330 - -0.9 429,590 + cobF COO64_RS14340 0.30 -0.6 429,591 - cobF COO64_RS14340 0.31 -0.3 429,591 - cobF COO64_RS14340 0.31 -0.5 429,635 + cobF COO64_RS14340 0.36 -0.1 429,635 + cobF COO64_RS14340 0.36 +0.1 429,635 + cobF COO64_RS14340 0.36 -0.6 429,636 - cobF COO64_RS14340 0.37 -1.5 429,636 - cobF COO64_RS14340 0.37 +0.1 429,636 - cobF COO64_RS14340 0.37 +1.1 429,711 - cobF COO64_RS14340 0.47 -0.5 429,711 - cobF COO64_RS14340 0.47 -0.6 429,742 - cobF COO64_RS14340 0.51 -0.7 429,836 - cobF COO64_RS14340 0.63 -0.4 429,962 + cobF COO64_RS14340 0.80 -0.5 429,962 + cobF COO64_RS14340 0.80 -0.8 429,962 + cobF COO64_RS14340 0.80 +0.1 429,962 + cobF COO64_RS14340 0.80 -0.6 429,962 + cobF COO64_RS14340 0.80 -0.8 429,963 - cobF COO64_RS14340 0.80 +0.9 429,974 + cobF COO64_RS14340 0.82 -1.0 430,088 + -1.0 430,088 + -0.7 430,088 + -0.8 430,088 + -0.1 430,088 + -0.0 430,088 + -0.1 430,089 - -0.9 430,089 - -0.7 430,089 - +1.1 430,089 - -0.0 430,089 - +1.0 430,089 - +0.3 430,147 + -0.3 430,148 - +0.7 430,148 - -1.7 430,340 - COO64_RS14345 0.20 -0.2 430,340 - COO64_RS14345 0.20 +0.1 430,473 - COO64_RS14345 0.30 -0.8 430,589 + COO64_RS14345 0.39 +1.1 430,590 - COO64_RS14345 0.39 -0.7 430,618 + COO64_RS14345 0.41 -0.6 430,618 + COO64_RS14345 0.41 -0.3 430,618 + COO64_RS14345 0.41 +0.1 430,618 + COO64_RS14345 0.41 -0.9 430,619 - COO64_RS14345 0.41 -0.1 430,619 - COO64_RS14345 0.41 +0.5 430,627 + COO64_RS14345 0.42 -0.5 430,627 + COO64_RS14345 0.42 -2.1 430,628 - COO64_RS14345 0.42 +0.8 430,628 - COO64_RS14345 0.42 -0.5 430,628 - COO64_RS14345 0.42 -0.2 430,628 - COO64_RS14345 0.42 -1.1 430,631 + COO64_RS14345 0.42 -1.1 430,631 + COO64_RS14345 0.42 -1.3 430,631 + COO64_RS14345 0.42 +0.1 430,631 + COO64_RS14345 0.42 +0.4 430,632 - COO64_RS14345 0.42 +0.5 430,632 - COO64_RS14345 0.42 -0.4 430,632 - COO64_RS14345 0.42 -1.5 430,632 - COO64_RS14345 0.42 -0.1 430,632 - COO64_RS14345 0.42 -0.3 430,891 + COO64_RS14345 0.62 +0.4 430,891 + COO64_RS14345 0.62 -0.0 430,891 + COO64_RS14345 0.62 -0.9 430,892 - COO64_RS14345 0.62 -0.9 430,892 - COO64_RS14345 0.62 +0.0 431,273 + +1.3 431,274 - +0.4 431,359 + +0.8 431,359 + -0.8 431,359 + -0.7 431,360 - -1.9 431,360 - +0.2 431,360 - -0.5 431,364 + -0.0 431,364 + -1.3 431,364 + -1.1 431,365 - -0.3 431,390 + +0.0 431,390 + -0.1 431,426 - -0.3 431,428 + -0.2 431,429 - -0.1 431,450 + -0.0 431,450 + +0.7 431,450 + +0.8 431,451 - -0.4 431,451 - -0.8 431,623 - COO64_RS14350 0.47 -0.2 431,852 + +1.0 431,852 + +0.3 431,853 - -0.9 431,853 - +0.8 431,853 - -0.3 431,853 - -0.5 431,853 - -0.6 431,853 - +1.4 431,886 + -1.0 431,895 + +0.5 431,918 + -1.8 431,918 + -1.1 431,927 - -0.0 431,956 + -0.9 431,956 + -1.2 432,006 - +0.4 432,146 + -0.2 432,146 + -0.3 432,147 - -0.5 432,147 - +0.9 432,156 - +0.7 432,234 + -0.2 432,234 + +0.4 432,235 - -0.2
Or see this region's nucleotide sequence