Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS18195 and COO64_RS18200 overlap by 4 nucleotides COO64_RS18200 and COO64_RS18205 overlap by 4 nucleotides COO64_RS18205 and COO64_RS18210 are separated by 178 nucleotides
COO64_RS18195: COO64_RS18195 - iron ABC transporter permease, at 170,214 to 171,236
_RS18195
COO64_RS18200: COO64_RS18200 - ABC transporter substrate-binding protein, at 171,233 to 172,183
_RS18200
COO64_RS18205: COO64_RS18205 - ABC transporter ATP-binding protein, at 172,180 to 172,968
_RS18205
COO64_RS18210: COO64_RS18210 - TonB-dependent copper receptor, at 173,147 to 175,213
_RS18210
Position (kb) 171
172
173 Strain fitness (log2 ratio) -1
0
1
2 at 170.877 kb on - strand, within COO64_RS18195 at 170.877 kb on - strand, within COO64_RS18195 at 170.892 kb on + strand, within COO64_RS18195 at 170.893 kb on - strand, within COO64_RS18195 at 171.047 kb on + strand, within COO64_RS18195 at 171.048 kb on - strand, within COO64_RS18195 at 171.048 kb on - strand, within COO64_RS18195 at 171.306 kb on + strand at 171.306 kb on + strand at 171.306 kb on + strand at 171.307 kb on - strand at 171.307 kb on - strand at 171.307 kb on - strand at 171.307 kb on - strand at 171.711 kb on + strand, within COO64_RS18200 at 171.711 kb on + strand, within COO64_RS18200 at 171.711 kb on + strand, within COO64_RS18200 at 171.711 kb on + strand, within COO64_RS18200 at 171.711 kb on + strand, within COO64_RS18200 at 171.711 kb on + strand, within COO64_RS18200 at 171.711 kb on + strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.712 kb on - strand, within COO64_RS18200 at 171.774 kb on + strand, within COO64_RS18200 at 171.775 kb on - strand, within COO64_RS18200 at 171.775 kb on - strand, within COO64_RS18200 at 171.837 kb on + strand, within COO64_RS18200 at 171.897 kb on + strand, within COO64_RS18200 at 171.897 kb on + strand, within COO64_RS18200 at 171.897 kb on + strand, within COO64_RS18200 at 171.898 kb on - strand, within COO64_RS18200 at 172.200 kb on + strand at 172.331 kb on + strand, within COO64_RS18205 at 172.331 kb on + strand, within COO64_RS18205 at 172.331 kb on + strand, within COO64_RS18205 at 172.331 kb on + strand, within COO64_RS18205 at 172.331 kb on + strand, within COO64_RS18205 at 172.331 kb on + strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.332 kb on - strand, within COO64_RS18205 at 172.940 kb on + strand at 172.940 kb on + strand at 172.941 kb on - strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.001 kb on + strand at 173.002 kb on - strand at 173.021 kb on - strand at 173.179 kb on + strand at 173.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X remove 170,877 - COO64_RS18195 0.65 -0.5 170,877 - COO64_RS18195 0.65 -0.1 170,892 + COO64_RS18195 0.66 -0.3 170,893 - COO64_RS18195 0.66 +0.9 171,047 + COO64_RS18195 0.81 +0.3 171,048 - COO64_RS18195 0.82 +0.9 171,048 - COO64_RS18195 0.82 +1.5 171,306 + +0.3 171,306 + -0.1 171,306 + +1.6 171,307 - -0.8 171,307 - +0.2 171,307 - +0.6 171,307 - -0.1 171,711 + COO64_RS18200 0.50 -0.7 171,711 + COO64_RS18200 0.50 -0.4 171,711 + COO64_RS18200 0.50 -1.1 171,711 + COO64_RS18200 0.50 -0.5 171,711 + COO64_RS18200 0.50 -1.2 171,711 + COO64_RS18200 0.50 +0.1 171,711 + COO64_RS18200 0.50 -1.7 171,712 - COO64_RS18200 0.50 +0.2 171,712 - COO64_RS18200 0.50 -0.5 171,712 - COO64_RS18200 0.50 +0.0 171,712 - COO64_RS18200 0.50 +0.5 171,712 - COO64_RS18200 0.50 +0.0 171,712 - COO64_RS18200 0.50 -0.1 171,712 - COO64_RS18200 0.50 -0.3 171,712 - COO64_RS18200 0.50 -0.1 171,712 - COO64_RS18200 0.50 -0.2 171,712 - COO64_RS18200 0.50 -0.7 171,712 - COO64_RS18200 0.50 +0.2 171,712 - COO64_RS18200 0.50 -0.2 171,774 + COO64_RS18200 0.57 +0.4 171,775 - COO64_RS18200 0.57 -0.6 171,775 - COO64_RS18200 0.57 +0.6 171,837 + COO64_RS18200 0.64 +0.2 171,897 + COO64_RS18200 0.70 -0.8 171,897 + COO64_RS18200 0.70 +0.6 171,897 + COO64_RS18200 0.70 -0.3 171,898 - COO64_RS18200 0.70 +0.3 172,200 + +1.6 172,331 + COO64_RS18205 0.19 -0.7 172,331 + COO64_RS18205 0.19 +0.5 172,331 + COO64_RS18205 0.19 +0.9 172,331 + COO64_RS18205 0.19 +0.1 172,331 + COO64_RS18205 0.19 -0.8 172,331 + COO64_RS18205 0.19 -0.3 172,332 - COO64_RS18205 0.19 -0.5 172,332 - COO64_RS18205 0.19 -0.1 172,332 - COO64_RS18205 0.19 -1.2 172,332 - COO64_RS18205 0.19 +0.2 172,332 - COO64_RS18205 0.19 -0.7 172,332 - COO64_RS18205 0.19 -0.1 172,332 - COO64_RS18205 0.19 -0.1 172,332 - COO64_RS18205 0.19 +0.3 172,332 - COO64_RS18205 0.19 +0.8 172,332 - COO64_RS18205 0.19 +0.4 172,940 + -0.3 172,940 + -0.2 172,941 - -0.0 173,001 + -0.2 173,001 + -0.4 173,001 + +0.3 173,001 + -0.3 173,001 + -0.2 173,001 + -1.1 173,001 + +0.2 173,001 + -0.9 173,002 - +0.5 173,021 - +0.2 173,179 + +1.9 173,180 - -0.7
Or see this region's nucleotide sequence