Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS21475 and vioA are separated by 202 nucleotides vioA and COO64_RS21485 overlap by 20 nucleotides COO64_RS21485 and COO64_RS21490 are separated by 118 nucleotides
COO64_RS21475: COO64_RS21475 - hypothetical protein, at 60,681 to 61,259
_RS21475
COO64_RS21480: vioA - dTDP-4-amino-4,6-dideoxy-D-glucose aminotransferase VioA, at 61,462 to 62,613
vioA
COO64_RS21485: COO64_RS21485 - LysR substrate-binding domain-containing protein, at 62,594 to 63,475
_RS21485
COO64_RS21490: COO64_RS21490 - glutathione S-transferase family protein, at 63,594 to 64,220
_RS21490
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.573 kb on + strand at 60.574 kb on - strand at 60.574 kb on - strand at 60.597 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.686 kb on + strand at 60.687 kb on - strand at 60.687 kb on - strand at 60.687 kb on - strand at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.975 kb on + strand, within COO64_RS21475 at 60.976 kb on - strand, within COO64_RS21475 at 60.976 kb on - strand, within COO64_RS21475 at 61.339 kb on - strand at 61.522 kb on + strand at 61.522 kb on + strand at 61.523 kb on - strand at 61.603 kb on + strand, within vioA at 61.604 kb on - strand, within vioA at 61.705 kb on + strand, within vioA at 61.705 kb on + strand, within vioA at 61.706 kb on - strand, within vioA at 61.706 kb on - strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.903 kb on + strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.904 kb on - strand, within vioA at 61.921 kb on + strand, within vioA at 61.921 kb on + strand, within vioA at 61.921 kb on + strand, within vioA at 61.921 kb on + strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 61.922 kb on - strand, within vioA at 62.080 kb on + strand, within vioA at 62.231 kb on - strand, within vioA at 62.253 kb on - strand, within vioA at 62.299 kb on + strand, within vioA at 62.299 kb on + strand, within vioA at 62.300 kb on - strand, within vioA at 62.300 kb on - strand, within vioA at 62.300 kb on - strand, within vioA at 62.300 kb on - strand, within vioA at 62.306 kb on - strand, within vioA at 62.306 kb on - strand, within vioA at 62.306 kb on - strand, within vioA at 62.360 kb on - strand, within vioA at 62.360 kb on - strand, within vioA at 62.398 kb on + strand, within vioA at 62.398 kb on + strand, within vioA at 62.399 kb on - strand, within vioA at 62.399 kb on - strand, within vioA at 62.404 kb on + strand, within vioA at 62.432 kb on - strand, within vioA at 62.495 kb on + strand, within vioA at 62.495 kb on + strand, within vioA at 62.496 kb on - strand, within vioA at 62.534 kb on + strand at 62.535 kb on - strand at 62.535 kb on - strand at 62.595 kb on + strand at 62.596 kb on - strand at 62.673 kb on + strand at 62.673 kb on + strand at 62.674 kb on - strand at 62.676 kb on + strand at 62.676 kb on + strand at 62.677 kb on - strand at 62.914 kb on - strand, within COO64_RS21485 at 62.988 kb on - strand, within COO64_RS21485 at 63.270 kb on + strand, within COO64_RS21485 at 63.479 kb on + strand at 63.479 kb on + strand at 63.480 kb on - strand at 63.480 kb on - strand at 63.480 kb on - strand at 63.553 kb on - strand at 63.553 kb on - strand at 63.557 kb on + strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.558 kb on - strand at 63.576 kb on + strand at 63.576 kb on + strand at 63.576 kb on + strand at 63.576 kb on + strand at 63.577 kb on - strand at 63.577 kb on - strand at 63.577 kb on - strand at 63.610 kb on - strand at 63.611 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X remove 60,573 + -0.8 60,574 - -0.7 60,574 - -0.5 60,597 + +0.5 60,686 + -0.2 60,686 + -0.5 60,686 + +0.6 60,686 + -1.8 60,686 + +0.0 60,686 + +1.3 60,687 - -2.0 60,687 - -0.4 60,687 - -0.0 60,975 + COO64_RS21475 0.51 +0.5 60,975 + COO64_RS21475 0.51 +0.1 60,975 + COO64_RS21475 0.51 -0.0 60,975 + COO64_RS21475 0.51 +0.1 60,975 + COO64_RS21475 0.51 +0.7 60,976 - COO64_RS21475 0.51 -0.4 60,976 - COO64_RS21475 0.51 -0.1 61,339 - +0.8 61,522 + -0.7 61,522 + +0.8 61,523 - +1.1 61,603 + vioA COO64_RS21480 0.12 +0.6 61,604 - vioA COO64_RS21480 0.12 +0.2 61,705 + vioA COO64_RS21480 0.21 -0.5 61,705 + vioA COO64_RS21480 0.21 -0.8 61,706 - vioA COO64_RS21480 0.21 +0.0 61,706 - vioA COO64_RS21480 0.21 +0.2 61,903 + vioA COO64_RS21480 0.38 +0.6 61,903 + vioA COO64_RS21480 0.38 +1.6 61,903 + vioA COO64_RS21480 0.38 -0.8 61,903 + vioA COO64_RS21480 0.38 +0.1 61,903 + vioA COO64_RS21480 0.38 +1.5 61,903 + vioA COO64_RS21480 0.38 -0.9 61,903 + vioA COO64_RS21480 0.38 +0.2 61,903 + vioA COO64_RS21480 0.38 +0.1 61,904 - vioA COO64_RS21480 0.38 -0.3 61,904 - vioA COO64_RS21480 0.38 +0.5 61,904 - vioA COO64_RS21480 0.38 -0.7 61,904 - vioA COO64_RS21480 0.38 +0.7 61,904 - vioA COO64_RS21480 0.38 -0.2 61,921 + vioA COO64_RS21480 0.40 +0.3 61,921 + vioA COO64_RS21480 0.40 -0.5 61,921 + vioA COO64_RS21480 0.40 +1.1 61,921 + vioA COO64_RS21480 0.40 +0.3 61,922 - vioA COO64_RS21480 0.40 -0.4 61,922 - vioA COO64_RS21480 0.40 -0.5 61,922 - vioA COO64_RS21480 0.40 +0.1 61,922 - vioA COO64_RS21480 0.40 -1.3 61,922 - vioA COO64_RS21480 0.40 +0.3 62,080 + vioA COO64_RS21480 0.54 -0.2 62,231 - vioA COO64_RS21480 0.67 -0.2 62,253 - vioA COO64_RS21480 0.69 -0.0 62,299 + vioA COO64_RS21480 0.73 -0.1 62,299 + vioA COO64_RS21480 0.73 -2.6 62,300 - vioA COO64_RS21480 0.73 +0.4 62,300 - vioA COO64_RS21480 0.73 +0.2 62,300 - vioA COO64_RS21480 0.73 -0.2 62,300 - vioA COO64_RS21480 0.73 -0.1 62,306 - vioA COO64_RS21480 0.73 -0.7 62,306 - vioA COO64_RS21480 0.73 -0.2 62,306 - vioA COO64_RS21480 0.73 +1.5 62,360 - vioA COO64_RS21480 0.78 -0.2 62,360 - vioA COO64_RS21480 0.78 -0.7 62,398 + vioA COO64_RS21480 0.81 -0.4 62,398 + vioA COO64_RS21480 0.81 +0.5 62,399 - vioA COO64_RS21480 0.81 -0.9 62,399 - vioA COO64_RS21480 0.81 +0.4 62,404 + vioA COO64_RS21480 0.82 -0.2 62,432 - vioA COO64_RS21480 0.84 -0.7 62,495 + vioA COO64_RS21480 0.90 +1.8 62,495 + vioA COO64_RS21480 0.90 -0.1 62,496 - vioA COO64_RS21480 0.90 +0.3 62,534 + +0.5 62,535 - +0.0 62,535 - -0.0 62,595 + -0.9 62,596 - -0.9 62,673 + -0.1 62,673 + -1.0 62,674 - -1.8 62,676 + +1.2 62,676 + +0.3 62,677 - -1.1 62,914 - COO64_RS21485 0.36 +0.9 62,988 - COO64_RS21485 0.45 -0.2 63,270 + COO64_RS21485 0.77 -0.3 63,479 + +0.5 63,479 + +1.1 63,480 - +0.6 63,480 - +1.0 63,480 - +0.1 63,553 - +0.4 63,553 - -0.0 63,557 + +0.3 63,558 - +0.8 63,558 - -1.6 63,558 - +0.3 63,558 - -1.0 63,558 - +0.2 63,558 - +0.1 63,558 - -1.2 63,558 - +0.5 63,558 - +0.0 63,576 + -0.1 63,576 + +0.8 63,576 + -0.9 63,576 + -0.9 63,577 - -0.2 63,577 - -0.0 63,577 - +0.1 63,610 - -0.1 63,611 + +0.4
Or see this region's nucleotide sequence