Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ettA and gdhA are separated by 243 nucleotides gdhA and COO64_RS03015 overlap by 4 nucleotides COO64_RS03015 and COO64_RS03020 are separated by 66 nucleotides
COO64_RS03005: ettA - energy-dependent translational throttle protein EttA, at 81,645 to 83,309
ettA
COO64_RS03010: gdhA - NADP-specific glutamate dehydrogenase, at 83,553 to 84,890
gdhA
COO64_RS03015: COO64_RS03015 - GreA/GreB family elongation factor, at 84,887 to 85,384
_RS03015
COO64_RS03020: COO64_RS03020 - hypothetical protein, at 85,451 to 85,807
_RS03020
Position (kb) 83
84
85 Strain fitness (log2 ratio) -2
-1
0
1 at 82.578 kb on + strand, within ettA at 82.578 kb on + strand, within ettA at 82.578 kb on + strand, within ettA at 82.578 kb on + strand, within ettA at 82.579 kb on - strand, within ettA at 82.579 kb on - strand, within ettA at 82.641 kb on + strand, within ettA at 82.642 kb on - strand, within ettA at 82.902 kb on + strand, within ettA at 82.903 kb on - strand, within ettA at 82.903 kb on - strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.923 kb on + strand, within ettA at 82.924 kb on - strand, within ettA at 82.924 kb on - strand, within ettA at 82.924 kb on - strand, within ettA at 83.184 kb on + strand at 83.185 kb on - strand at 83.224 kb on - strand at 83.224 kb on - strand at 83.224 kb on - strand at 83.232 kb on + strand at 83.233 kb on - strand at 83.308 kb on - strand at 83.308 kb on - strand at 83.308 kb on - strand at 83.407 kb on + strand at 83.408 kb on - strand at 83.408 kb on - strand at 83.503 kb on + strand at 83.503 kb on + strand at 83.504 kb on - strand at 83.504 kb on - strand at 83.524 kb on + strand at 83.525 kb on - strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.550 kb on + strand at 83.551 kb on - strand at 83.551 kb on - strand at 83.551 kb on - strand at 83.679 kb on + strand at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.706 kb on - strand, within gdhA at 83.817 kb on + strand, within gdhA at 83.817 kb on + strand, within gdhA at 83.817 kb on + strand, within gdhA at 83.818 kb on - strand, within gdhA at 83.818 kb on - strand, within gdhA at 83.818 kb on - strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.093 kb on + strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.094 kb on - strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.147 kb on + strand, within gdhA at 84.148 kb on - strand, within gdhA at 84.195 kb on + strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.196 kb on - strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.282 kb on + strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.283 kb on - strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.351 kb on + strand, within gdhA at 84.352 kb on - strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.617 kb on + strand, within gdhA at 84.792 kb on + strand at 84.793 kb on - strand at 84.793 kb on - strand at 84.819 kb on + strand at 84.820 kb on - strand at 84.820 kb on - strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.888 kb on + strand at 84.889 kb on - strand at 84.889 kb on - strand at 84.889 kb on - strand at 85.026 kb on + strand, within COO64_RS03015 at 85.026 kb on + strand, within COO64_RS03015 at 85.027 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.168 kb on - strand, within COO64_RS03015 at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.452 kb on + strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.453 kb on - strand at 85.537 kb on - strand, within COO64_RS03020 at 85.605 kb on + strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.606 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.624 kb on - strand, within COO64_RS03020 at 85.778 kb on + strand at 85.779 kb on - strand at 85.838 kb on + strand at 85.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X remove 82,578 + ettA COO64_RS03005 0.56 -0.3 82,578 + ettA COO64_RS03005 0.56 +0.0 82,578 + ettA COO64_RS03005 0.56 -0.1 82,578 + ettA COO64_RS03005 0.56 -1.3 82,579 - ettA COO64_RS03005 0.56 -0.1 82,579 - ettA COO64_RS03005 0.56 +0.2 82,641 + ettA COO64_RS03005 0.60 -1.8 82,642 - ettA COO64_RS03005 0.60 -0.2 82,902 + ettA COO64_RS03005 0.75 -0.8 82,903 - ettA COO64_RS03005 0.76 +0.0 82,903 - ettA COO64_RS03005 0.76 -0.5 82,923 + ettA COO64_RS03005 0.77 -0.3 82,923 + ettA COO64_RS03005 0.77 -0.5 82,923 + ettA COO64_RS03005 0.77 +0.4 82,923 + ettA COO64_RS03005 0.77 -0.3 82,923 + ettA COO64_RS03005 0.77 -0.1 82,923 + ettA COO64_RS03005 0.77 -0.6 82,924 - ettA COO64_RS03005 0.77 +0.0 82,924 - ettA COO64_RS03005 0.77 -0.9 82,924 - ettA COO64_RS03005 0.77 +0.6 83,184 + -0.5 83,185 - -0.0 83,224 - -0.1 83,224 - -0.2 83,224 - +0.2 83,232 + +0.9 83,233 - +1.2 83,308 - +0.9 83,308 - +0.0 83,308 - -0.3 83,407 + +0.3 83,408 - +0.6 83,408 - +1.2 83,503 + +0.8 83,503 + +0.2 83,504 - +1.2 83,504 - +0.9 83,524 + -0.4 83,525 - +0.2 83,550 + +1.3 83,550 + -0.2 83,550 + +0.9 83,550 + +0.5 83,550 + -0.1 83,550 + +0.2 83,550 + -1.8 83,551 - +0.2 83,551 - -0.6 83,551 - +0.3 83,679 + -0.6 83,706 - gdhA COO64_RS03010 0.11 +0.4 83,706 - gdhA COO64_RS03010 0.11 -1.1 83,706 - gdhA COO64_RS03010 0.11 -0.1 83,706 - gdhA COO64_RS03010 0.11 -0.2 83,706 - gdhA COO64_RS03010 0.11 +1.3 83,706 - gdhA COO64_RS03010 0.11 -1.6 83,817 + gdhA COO64_RS03010 0.20 +0.2 83,817 + gdhA COO64_RS03010 0.20 -0.3 83,817 + gdhA COO64_RS03010 0.20 +0.9 83,818 - gdhA COO64_RS03010 0.20 +0.5 83,818 - gdhA COO64_RS03010 0.20 -1.1 83,818 - gdhA COO64_RS03010 0.20 +0.5 84,093 + gdhA COO64_RS03010 0.40 +0.4 84,093 + gdhA COO64_RS03010 0.40 -1.8 84,093 + gdhA COO64_RS03010 0.40 +0.0 84,094 - gdhA COO64_RS03010 0.40 -0.9 84,094 - gdhA COO64_RS03010 0.40 +0.9 84,094 - gdhA COO64_RS03010 0.40 -0.5 84,147 + gdhA COO64_RS03010 0.44 +1.1 84,147 + gdhA COO64_RS03010 0.44 -2.1 84,148 - gdhA COO64_RS03010 0.44 +0.1 84,195 + gdhA COO64_RS03010 0.48 -0.0 84,196 - gdhA COO64_RS03010 0.48 +0.3 84,196 - gdhA COO64_RS03010 0.48 +0.8 84,196 - gdhA COO64_RS03010 0.48 +1.0 84,282 + gdhA COO64_RS03010 0.54 -1.5 84,282 + gdhA COO64_RS03010 0.54 -0.7 84,283 - gdhA COO64_RS03010 0.55 -0.3 84,283 - gdhA COO64_RS03010 0.55 -0.2 84,351 + gdhA COO64_RS03010 0.60 +1.1 84,351 + gdhA COO64_RS03010 0.60 +0.2 84,351 + gdhA COO64_RS03010 0.60 -0.0 84,351 + gdhA COO64_RS03010 0.60 +0.7 84,352 - gdhA COO64_RS03010 0.60 -0.6 84,617 + gdhA COO64_RS03010 0.80 -0.1 84,617 + gdhA COO64_RS03010 0.80 -0.6 84,792 + +0.4 84,793 - +0.1 84,793 - -0.1 84,819 + -1.1 84,820 - +1.0 84,820 - +0.3 84,888 + +0.2 84,888 + -0.4 84,888 + -1.2 84,888 + +0.9 84,889 - +1.3 84,889 - -0.3 84,889 - +0.1 85,026 + COO64_RS03015 0.28 -0.0 85,026 + COO64_RS03015 0.28 -1.1 85,027 - COO64_RS03015 0.28 +0.5 85,168 - COO64_RS03015 0.56 -0.1 85,168 - COO64_RS03015 0.56 +0.9 85,452 + +1.6 85,452 + +1.4 85,452 + -0.1 85,452 + -0.7 85,452 + -0.7 85,452 + +0.5 85,452 + +0.3 85,453 - -0.7 85,453 - +1.0 85,453 - -0.7 85,453 - +0.2 85,537 - COO64_RS03020 0.24 -0.1 85,605 + COO64_RS03020 0.43 -0.5 85,606 - COO64_RS03020 0.43 +0.3 85,606 - COO64_RS03020 0.43 -1.7 85,624 - COO64_RS03020 0.48 +0.3 85,624 - COO64_RS03020 0.48 -1.1 85,778 + +0.2 85,779 - -0.6 85,838 + +0.1 85,839 - +0.7
Or see this region's nucleotide sequence