Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS03550 and COO64_RS03555 are separated by 9 nucleotides COO64_RS03555 and COO64_RS03560 overlap by 17 nucleotides COO64_RS03560 and COO64_RS03565 overlap by 8 nucleotides COO64_RS03565 and folM are separated by 24 nucleotides
COO64_RS03550: COO64_RS03550 - GNAT family N-acetyltransferase, at 191,021 to 191,707
_RS03550
COO64_RS03555: COO64_RS03555 - alpha/beta fold hydrolase, at 191,717 to 192,670
_RS03555
COO64_RS03560: COO64_RS03560 - HopJ type III effector protein, at 192,654 to 192,995
_RS03560
COO64_RS03565: COO64_RS03565 - DUF1244 domain-containing protein, at 192,988 to 193,284
_RS03565
COO64_RS03570: folM - dihydromonapterin reductase, at 193,309 to 194,007
folM
Position (kb)
192
193 Strain fitness (log2 ratio)
-2
-1
0
1 at 191.751 kb on + strand at 191.891 kb on + strand, within COO64_RS03555 at 191.891 kb on + strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.892 kb on - strand, within COO64_RS03555 at 191.894 kb on + strand, within COO64_RS03555 at 191.894 kb on + strand, within COO64_RS03555 at 191.895 kb on - strand, within COO64_RS03555 at 191.895 kb on - strand, within COO64_RS03555 at 191.912 kb on + strand, within COO64_RS03555 at 191.913 kb on - strand, within COO64_RS03555 at 191.913 kb on - strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.945 kb on + strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.946 kb on - strand, within COO64_RS03555 at 191.952 kb on - strand, within COO64_RS03555 at 191.952 kb on - strand, within COO64_RS03555 at 191.952 kb on - strand, within COO64_RS03555 at 191.952 kb on - strand, within COO64_RS03555 at 192.177 kb on - strand, within COO64_RS03555 at 192.177 kb on - strand, within COO64_RS03555 at 192.188 kb on + strand, within COO64_RS03555 at 192.189 kb on - strand, within COO64_RS03555 at 192.264 kb on - strand, within COO64_RS03555 at 192.298 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.344 kb on + strand, within COO64_RS03555 at 192.345 kb on - strand, within COO64_RS03555 at 192.345 kb on - strand, within COO64_RS03555 at 192.475 kb on - strand, within COO64_RS03555 at 192.475 kb on - strand, within COO64_RS03555 at 192.483 kb on - strand, within COO64_RS03555 at 192.483 kb on - strand, within COO64_RS03555 at 192.483 kb on - strand, within COO64_RS03555 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.904 kb on + strand, within COO64_RS03560 at 192.905 kb on - strand, within COO64_RS03560 at 192.905 kb on - strand, within COO64_RS03560 at 192.905 kb on - strand, within COO64_RS03560 at 192.905 kb on - strand, within COO64_RS03560 at 192.905 kb on - strand, within COO64_RS03560 at 192.910 kb on + strand, within COO64_RS03560 at 192.910 kb on + strand, within COO64_RS03560 at 192.910 kb on + strand, within COO64_RS03560 at 192.910 kb on + strand, within COO64_RS03560 at 192.910 kb on + strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 192.911 kb on - strand, within COO64_RS03560 at 193.049 kb on + strand, within COO64_RS03565 at 193.049 kb on + strand, within COO64_RS03565 at 193.070 kb on + strand, within COO64_RS03565 at 193.070 kb on + strand, within COO64_RS03565 at 193.070 kb on + strand, within COO64_RS03565 at 193.071 kb on - strand, within COO64_RS03565 at 193.082 kb on + strand, within COO64_RS03565 at 193.082 kb on + strand, within COO64_RS03565 at 193.082 kb on + strand, within COO64_RS03565 at 193.083 kb on - strand, within COO64_RS03565 at 193.145 kb on + strand, within COO64_RS03565 at 193.145 kb on + strand, within COO64_RS03565 at 193.145 kb on + strand, within COO64_RS03565 at 193.145 kb on + strand, within COO64_RS03565 at 193.145 kb on + strand, within COO64_RS03565 at 193.146 kb on - strand, within COO64_RS03565 at 193.146 kb on - strand, within COO64_RS03565 at 193.146 kb on - strand, within COO64_RS03565 at 193.146 kb on - strand, within COO64_RS03565 at 193.264 kb on + strand at 193.264 kb on + strand at 193.264 kb on + strand at 193.264 kb on + strand at 193.264 kb on + strand at 193.264 kb on + strand at 193.265 kb on - strand at 193.265 kb on - strand at 193.265 kb on - strand at 193.305 kb on + strand at 193.306 kb on - strand at 193.306 kb on - strand at 193.306 kb on - strand at 193.306 kb on - strand at 193.376 kb on + strand at 193.376 kb on + strand at 193.376 kb on + strand at 193.376 kb on + strand at 193.376 kb on + strand at 193.377 kb on - strand at 193.541 kb on + strand, within folM at 193.541 kb on + strand, within folM at 193.541 kb on + strand, within folM at 193.542 kb on - strand, within folM at 193.542 kb on - strand, within folM at 193.596 kb on - strand, within folM at 193.596 kb on - strand, within folM at 193.703 kb on + strand, within folM at 193.704 kb on - strand, within folM at 193.704 kb on - strand, within folM at 193.803 kb on - strand, within folM at 193.803 kb on - strand, within folM at 193.927 kb on - strand, within folM
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.25X remove 191,751 + +0.1 191,891 + COO64_RS03555 0.18 -0.1 191,891 + COO64_RS03555 0.18 +1.4 191,892 - COO64_RS03555 0.18 -0.2 191,892 - COO64_RS03555 0.18 -0.3 191,892 - COO64_RS03555 0.18 -0.1 191,892 - COO64_RS03555 0.18 +0.2 191,892 - COO64_RS03555 0.18 -0.3 191,892 - COO64_RS03555 0.18 +0.2 191,892 - COO64_RS03555 0.18 -2.2 191,892 - COO64_RS03555 0.18 +0.7 191,892 - COO64_RS03555 0.18 -1.0 191,892 - COO64_RS03555 0.18 -0.2 191,894 + COO64_RS03555 0.19 +0.2 191,894 + COO64_RS03555 0.19 -0.8 191,895 - COO64_RS03555 0.19 -0.3 191,895 - COO64_RS03555 0.19 -0.4 191,912 + COO64_RS03555 0.20 -0.1 191,913 - COO64_RS03555 0.21 +0.6 191,913 - COO64_RS03555 0.21 +0.9 191,945 + COO64_RS03555 0.24 +0.1 191,945 + COO64_RS03555 0.24 -0.1 191,945 + COO64_RS03555 0.24 +0.6 191,945 + COO64_RS03555 0.24 -0.9 191,945 + COO64_RS03555 0.24 +0.0 191,945 + COO64_RS03555 0.24 -0.9 191,945 + COO64_RS03555 0.24 -1.2 191,945 + COO64_RS03555 0.24 -0.1 191,945 + COO64_RS03555 0.24 +0.9 191,946 - COO64_RS03555 0.24 -1.0 191,946 - COO64_RS03555 0.24 -0.3 191,946 - COO64_RS03555 0.24 +0.4 191,946 - COO64_RS03555 0.24 -0.0 191,946 - COO64_RS03555 0.24 +0.3 191,946 - COO64_RS03555 0.24 +1.2 191,946 - COO64_RS03555 0.24 +0.4 191,946 - COO64_RS03555 0.24 -0.2 191,946 - COO64_RS03555 0.24 -0.2 191,952 - COO64_RS03555 0.25 -0.6 191,952 - COO64_RS03555 0.25 +0.2 191,952 - COO64_RS03555 0.25 -0.6 191,952 - COO64_RS03555 0.25 -0.9 192,177 - COO64_RS03555 0.48 +0.1 192,177 - COO64_RS03555 0.48 -0.6 192,188 + COO64_RS03555 0.49 -0.9 192,189 - COO64_RS03555 0.49 -1.0 192,264 - COO64_RS03555 0.57 +0.1 192,298 + COO64_RS03555 0.61 +1.3 192,344 + COO64_RS03555 0.66 -0.5 192,344 + COO64_RS03555 0.66 +0.3 192,344 + COO64_RS03555 0.66 -0.1 192,344 + COO64_RS03555 0.66 +0.9 192,344 + COO64_RS03555 0.66 -1.0 192,344 + COO64_RS03555 0.66 +0.2 192,344 + COO64_RS03555 0.66 +0.1 192,345 - COO64_RS03555 0.66 +0.4 192,345 - COO64_RS03555 0.66 -0.3 192,475 - COO64_RS03555 0.79 +0.1 192,475 - COO64_RS03555 0.79 +0.0 192,483 - COO64_RS03555 0.80 +0.1 192,483 - COO64_RS03555 0.80 +0.7 192,483 - COO64_RS03555 0.80 +0.6 192,904 + COO64_RS03560 0.73 +1.2 192,904 + COO64_RS03560 0.73 +0.3 192,904 + COO64_RS03560 0.73 +1.0 192,904 + COO64_RS03560 0.73 -1.0 192,904 + COO64_RS03560 0.73 -0.2 192,904 + COO64_RS03560 0.73 +0.4 192,904 + COO64_RS03560 0.73 -0.9 192,904 + COO64_RS03560 0.73 +0.2 192,904 + COO64_RS03560 0.73 +0.1 192,904 + COO64_RS03560 0.73 +0.4 192,905 - COO64_RS03560 0.73 -0.0 192,905 - COO64_RS03560 0.73 -0.9 192,905 - COO64_RS03560 0.73 +0.5 192,905 - COO64_RS03560 0.73 -0.0 192,905 - COO64_RS03560 0.73 +0.1 192,910 + COO64_RS03560 0.75 -0.1 192,910 + COO64_RS03560 0.75 +0.1 192,910 + COO64_RS03560 0.75 +0.6 192,910 + COO64_RS03560 0.75 +0.1 192,910 + COO64_RS03560 0.75 +0.3 192,911 - COO64_RS03560 0.75 -1.3 192,911 - COO64_RS03560 0.75 +0.2 192,911 - COO64_RS03560 0.75 -0.4 192,911 - COO64_RS03560 0.75 +0.0 192,911 - COO64_RS03560 0.75 +0.4 192,911 - COO64_RS03560 0.75 +1.3 192,911 - COO64_RS03560 0.75 -0.6 193,049 + COO64_RS03565 0.21 -0.2 193,049 + COO64_RS03565 0.21 -0.7 193,070 + COO64_RS03565 0.28 -1.9 193,070 + COO64_RS03565 0.28 -0.1 193,070 + COO64_RS03565 0.28 -1.6 193,071 - COO64_RS03565 0.28 -0.6 193,082 + COO64_RS03565 0.32 -0.5 193,082 + COO64_RS03565 0.32 +0.0 193,082 + COO64_RS03565 0.32 -1.1 193,083 - COO64_RS03565 0.32 -0.2 193,145 + COO64_RS03565 0.53 +0.7 193,145 + COO64_RS03565 0.53 -0.6 193,145 + COO64_RS03565 0.53 -0.5 193,145 + COO64_RS03565 0.53 +0.0 193,145 + COO64_RS03565 0.53 -0.0 193,146 - COO64_RS03565 0.53 +1.0 193,146 - COO64_RS03565 0.53 -0.8 193,146 - COO64_RS03565 0.53 +1.2 193,146 - COO64_RS03565 0.53 -0.8 193,264 + -0.0 193,264 + -0.9 193,264 + -0.0 193,264 + +0.0 193,264 + +1.0 193,264 + +0.4 193,265 - -0.7 193,265 - -1.5 193,265 - -0.8 193,305 + -1.0 193,306 - -0.1 193,306 - +0.1 193,306 - -0.3 193,306 - +0.8 193,376 + -0.1 193,376 + -0.9 193,376 + +0.6 193,376 + +0.3 193,376 + -0.1 193,377 - +1.1 193,541 + folM COO64_RS03570 0.33 +0.1 193,541 + folM COO64_RS03570 0.33 -0.5 193,541 + folM COO64_RS03570 0.33 -0.2 193,542 - folM COO64_RS03570 0.33 +0.0 193,542 - folM COO64_RS03570 0.33 +0.4 193,596 - folM COO64_RS03570 0.41 +0.0 193,596 - folM COO64_RS03570 0.41 +0.8 193,703 + folM COO64_RS03570 0.56 -0.3 193,704 - folM COO64_RS03570 0.57 -0.9 193,704 - folM COO64_RS03570 0.57 -0.2 193,803 - folM COO64_RS03570 0.71 +0.3 193,803 - folM COO64_RS03570 0.71 -0.2 193,927 - folM COO64_RS03570 0.88 -0.6
Or see this region's nucleotide sequence