Experiment: supernatant control, M9 with 0.1X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS01015 and COO64_RS26980 are separated by 18 nucleotides COO64_RS26980 and COO64_RS01020 are separated by 178 nucleotides COO64_RS01020 and COO64_RS01025 are separated by 93 nucleotides COO64_RS01025 and rbbA overlap by 4 nucleotides
COO64_RS01015: COO64_RS01015 - 5-formyltetrahydrofolate cyclo-ligase, at 216,243 to 216,851
_RS01015
COO64_RS26980: COO64_RS26980 - hypothetical protein, at 216,870 to 217,010
_RS26980
COO64_RS01020: COO64_RS01020 - EVE domain-containing protein, at 217,189 to 217,638
_RS01020
COO64_RS01025: COO64_RS01025 - HlyD family secretion protein, at 217,732 to 218,697
_RS01025
COO64_RS01030: rbbA - ribosome-associated ATPase/putative transporter RbbA, at 218,694 to 221,417
rbbA
Position (kb)
217
218
219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 216.894 kb on + strand, within COO64_RS26980 at 216.901 kb on + strand, within COO64_RS26980 at 216.940 kb on + strand, within COO64_RS26980 at 216.947 kb on + strand, within COO64_RS26980 at 216.948 kb on - strand, within COO64_RS26980 at 217.080 kb on + strand at 217.080 kb on + strand at 217.081 kb on - strand at 217.160 kb on + strand at 217.160 kb on + strand at 217.161 kb on - strand at 217.161 kb on - strand at 217.195 kb on + strand at 217.195 kb on + strand at 217.196 kb on - strand at 217.196 kb on - strand at 217.282 kb on + strand, within COO64_RS01020 at 217.282 kb on + strand, within COO64_RS01020 at 217.293 kb on + strand, within COO64_RS01020 at 217.336 kb on + strand, within COO64_RS01020 at 217.336 kb on + strand, within COO64_RS01020 at 217.337 kb on - strand, within COO64_RS01020 at 217.397 kb on - strand, within COO64_RS01020 at 217.432 kb on + strand, within COO64_RS01020 at 217.432 kb on + strand, within COO64_RS01020 at 217.432 kb on + strand, within COO64_RS01020 at 217.432 kb on + strand, within COO64_RS01020 at 217.432 kb on + strand, within COO64_RS01020 at 217.433 kb on - strand, within COO64_RS01020 at 217.433 kb on - strand, within COO64_RS01020 at 217.525 kb on + strand, within COO64_RS01020 at 217.526 kb on - strand, within COO64_RS01020 at 217.526 kb on - strand, within COO64_RS01020 at 217.565 kb on + strand, within COO64_RS01020 at 217.645 kb on + strand at 217.645 kb on + strand at 217.645 kb on + strand at 217.711 kb on - strand at 217.811 kb on - strand at 218.041 kb on + strand, within COO64_RS01025 at 218.137 kb on + strand, within COO64_RS01025 at 218.137 kb on + strand, within COO64_RS01025 at 218.137 kb on + strand, within COO64_RS01025 at 218.176 kb on + strand, within COO64_RS01025 at 218.327 kb on + strand, within COO64_RS01025 at 218.327 kb on + strand, within COO64_RS01025 at 218.328 kb on - strand, within COO64_RS01025 at 218.328 kb on - strand, within COO64_RS01025 at 218.328 kb on - strand, within COO64_RS01025 at 218.328 kb on - strand, within COO64_RS01025 at 218.328 kb on - strand, within COO64_RS01025 at 218.328 kb on - strand, within COO64_RS01025 at 218.372 kb on + strand, within COO64_RS01025 at 218.373 kb on - strand, within COO64_RS01025 at 218.373 kb on - strand, within COO64_RS01025 at 218.401 kb on + strand, within COO64_RS01025 at 218.401 kb on + strand, within COO64_RS01025 at 218.401 kb on + strand, within COO64_RS01025 at 218.401 kb on + strand, within COO64_RS01025 at 218.401 kb on + strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.402 kb on - strand, within COO64_RS01025 at 218.413 kb on + strand, within COO64_RS01025 at 218.413 kb on + strand, within COO64_RS01025 at 218.413 kb on + strand, within COO64_RS01025 at 218.414 kb on - strand, within COO64_RS01025 at 218.414 kb on - strand, within COO64_RS01025 at 218.653 kb on + strand at 218.668 kb on - strand at 218.668 kb on - strand at 218.736 kb on + strand at 218.918 kb on + strand at 218.929 kb on - strand at 218.974 kb on - strand, within rbbA at 219.294 kb on + strand, within rbbA at 219.294 kb on + strand, within rbbA at 219.294 kb on + strand, within rbbA at 219.295 kb on - strand, within rbbA at 219.295 kb on - strand, within rbbA at 219.295 kb on - strand, within rbbA at 219.295 kb on - strand, within rbbA at 219.476 kb on + strand, within rbbA at 219.673 kb on + strand, within rbbA
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.1X LB remove 216,894 + COO64_RS26980 0.17 +0.9 216,901 + COO64_RS26980 0.22 -0.0 216,940 + COO64_RS26980 0.50 +0.2 216,947 + COO64_RS26980 0.55 -1.1 216,948 - COO64_RS26980 0.55 +0.8 217,080 + -0.2 217,080 + +0.9 217,081 - +0.1 217,160 + -0.4 217,160 + +0.1 217,161 - +0.4 217,161 - -1.1 217,195 + +0.4 217,195 + -1.1 217,196 - +0.3 217,196 - +0.8 217,282 + COO64_RS01020 0.21 +0.3 217,282 + COO64_RS01020 0.21 -0.1 217,293 + COO64_RS01020 0.23 +1.2 217,336 + COO64_RS01020 0.33 +0.5 217,336 + COO64_RS01020 0.33 +0.0 217,337 - COO64_RS01020 0.33 +0.7 217,397 - COO64_RS01020 0.46 -0.2 217,432 + COO64_RS01020 0.54 -2.2 217,432 + COO64_RS01020 0.54 -0.7 217,432 + COO64_RS01020 0.54 +0.6 217,432 + COO64_RS01020 0.54 +0.6 217,432 + COO64_RS01020 0.54 -0.5 217,433 - COO64_RS01020 0.54 -2.0 217,433 - COO64_RS01020 0.54 +0.2 217,525 + COO64_RS01020 0.75 -0.8 217,526 - COO64_RS01020 0.75 +0.6 217,526 - COO64_RS01020 0.75 -0.9 217,565 + COO64_RS01020 0.84 +0.4 217,645 + -0.7 217,645 + -0.7 217,645 + +0.1 217,711 - -0.3 217,811 - +1.1 218,041 + COO64_RS01025 0.32 -1.5 218,137 + COO64_RS01025 0.42 +0.9 218,137 + COO64_RS01025 0.42 +0.1 218,137 + COO64_RS01025 0.42 +0.1 218,176 + COO64_RS01025 0.46 +0.9 218,327 + COO64_RS01025 0.62 +0.1 218,327 + COO64_RS01025 0.62 +0.5 218,328 - COO64_RS01025 0.62 +0.2 218,328 - COO64_RS01025 0.62 -1.0 218,328 - COO64_RS01025 0.62 -0.2 218,328 - COO64_RS01025 0.62 -2.2 218,328 - COO64_RS01025 0.62 +0.5 218,328 - COO64_RS01025 0.62 +0.2 218,372 + COO64_RS01025 0.66 -0.8 218,373 - COO64_RS01025 0.66 +1.3 218,373 - COO64_RS01025 0.66 +0.7 218,401 + COO64_RS01025 0.69 -1.0 218,401 + COO64_RS01025 0.69 -1.0 218,401 + COO64_RS01025 0.69 +0.2 218,401 + COO64_RS01025 0.69 +0.1 218,401 + COO64_RS01025 0.69 +1.0 218,402 - COO64_RS01025 0.69 +0.1 218,402 - COO64_RS01025 0.69 -0.5 218,402 - COO64_RS01025 0.69 +1.0 218,402 - COO64_RS01025 0.69 +0.0 218,402 - COO64_RS01025 0.69 -2.3 218,402 - COO64_RS01025 0.69 +1.2 218,402 - COO64_RS01025 0.69 -1.4 218,413 + COO64_RS01025 0.70 +0.5 218,413 + COO64_RS01025 0.70 +0.0 218,413 + COO64_RS01025 0.70 -1.1 218,414 - COO64_RS01025 0.71 +0.6 218,414 - COO64_RS01025 0.71 +0.9 218,653 + -1.0 218,668 - -0.1 218,668 - -0.2 218,736 + +0.1 218,918 + -0.7 218,929 - +1.8 218,974 - rbbA COO64_RS01030 0.10 +2.4 219,294 + rbbA COO64_RS01030 0.22 -0.1 219,294 + rbbA COO64_RS01030 0.22 +0.9 219,294 + rbbA COO64_RS01030 0.22 -0.7 219,295 - rbbA COO64_RS01030 0.22 +0.3 219,295 - rbbA COO64_RS01030 0.22 +0.2 219,295 - rbbA COO64_RS01030 0.22 -1.1 219,295 - rbbA COO64_RS01030 0.22 +0.3 219,476 + rbbA COO64_RS01030 0.29 -0.2 219,673 + rbbA COO64_RS01030 0.36 +0.5
Or see this region's nucleotide sequence