Experiment: supernatant control, M9 with 0.1X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS14475 and COO64_RS14480 are separated by 30 nucleotides COO64_RS14480 and COO64_RS14485 are separated by 144 nucleotides COO64_RS14485 and COO64_RS14490 are separated by 84 nucleotides
COO64_RS14475: COO64_RS14475 - DMT family transporter, at 457,532 to 458,476
_RS14475
COO64_RS14480: COO64_RS14480 - hypothetical protein, at 458,507 to 458,893
_RS14480
COO64_RS14485: COO64_RS14485 - MOSC domain-containing protein, at 459,038 to 459,742
_RS14485
COO64_RS14490: COO64_RS14490 - gamma-glutamyl-gamma-aminobutyrate hydrolase family protein, at 459,827 to 460,642
_RS14490
Position (kb) 459
460 Strain fitness (log2 ratio) -2
-1
0
1
2 at 458.155 kb on - strand, within COO64_RS14475 at 458.234 kb on + strand, within COO64_RS14475 at 458.234 kb on + strand, within COO64_RS14475 at 458.471 kb on - strand at 458.471 kb on - strand at 458.471 kb on - strand at 458.474 kb on + strand at 458.474 kb on + strand at 458.475 kb on - strand at 458.475 kb on - strand at 458.475 kb on - strand at 458.475 kb on - strand at 458.715 kb on + strand, within COO64_RS14480 at 458.715 kb on + strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.716 kb on - strand, within COO64_RS14480 at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.959 kb on + strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.960 kb on - strand at 458.963 kb on + strand at 459.021 kb on - strand at 459.021 kb on - strand at 459.069 kb on + strand at 459.069 kb on + strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.070 kb on - strand at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.245 kb on + strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.246 kb on - strand, within COO64_RS14485 at 459.345 kb on + strand, within COO64_RS14485 at 459.345 kb on + strand, within COO64_RS14485 at 459.529 kb on + strand, within COO64_RS14485 at 459.758 kb on + strand at 459.758 kb on + strand at 459.759 kb on - strand at 459.759 kb on - strand at 459.759 kb on - strand at 459.792 kb on + strand at 459.792 kb on + strand at 459.793 kb on - strand at 459.793 kb on - strand at 460.155 kb on + strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.156 kb on - strand, within COO64_RS14490 at 460.216 kb on - strand, within COO64_RS14490 at 460.228 kb on - strand, within COO64_RS14490 at 460.313 kb on + strand, within COO64_RS14490 at 460.313 kb on + strand, within COO64_RS14490 at 460.446 kb on + strand, within COO64_RS14490 at 460.493 kb on + strand, within COO64_RS14490 at 460.493 kb on + strand, within COO64_RS14490 at 460.494 kb on - strand, within COO64_RS14490 at 460.494 kb on - strand, within COO64_RS14490 at 460.494 kb on - strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.527 kb on + strand, within COO64_RS14490 at 460.528 kb on - strand, within COO64_RS14490 at 460.528 kb on - strand, within COO64_RS14490 at 460.528 kb on - strand, within COO64_RS14490 at 460.549 kb on - strand, within COO64_RS14490 at 460.618 kb on + strand at 460.619 kb on - strand at 460.619 kb on - strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.642 kb on + strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.643 kb on - strand at 460.674 kb on + strand at 460.675 kb on - strand at 460.675 kb on - strand at 460.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.1X LB remove 458,155 - COO64_RS14475 0.66 +0.7 458,234 + COO64_RS14475 0.74 +1.5 458,234 + COO64_RS14475 0.74 -0.4 458,471 - +1.0 458,471 - -0.2 458,471 - -0.9 458,474 + +0.0 458,474 + +0.7 458,475 - -0.3 458,475 - +0.0 458,475 - -1.5 458,475 - -1.1 458,715 + COO64_RS14480 0.54 -1.7 458,715 + COO64_RS14480 0.54 -1.0 458,716 - COO64_RS14480 0.54 -1.3 458,716 - COO64_RS14480 0.54 -1.2 458,716 - COO64_RS14480 0.54 +0.2 458,716 - COO64_RS14480 0.54 +0.3 458,959 + +0.8 458,959 + +0.4 458,959 + +1.2 458,959 + +0.1 458,959 + +1.5 458,959 + +1.0 458,960 - -0.4 458,960 - -1.5 458,960 - +0.4 458,960 - +0.7 458,960 - +1.3 458,960 - -1.2 458,960 - +0.1 458,963 + +0.1 459,021 - -2.5 459,021 - +0.5 459,069 + -0.1 459,069 + +0.9 459,070 - +0.1 459,070 - -0.1 459,070 - +0.8 459,070 - +0.8 459,070 - -0.4 459,245 + COO64_RS14485 0.29 -0.9 459,245 + COO64_RS14485 0.29 +0.3 459,245 + COO64_RS14485 0.29 -1.4 459,245 + COO64_RS14485 0.29 -0.5 459,245 + COO64_RS14485 0.29 +0.0 459,245 + COO64_RS14485 0.29 -0.1 459,245 + COO64_RS14485 0.29 -0.7 459,245 + COO64_RS14485 0.29 -0.2 459,245 + COO64_RS14485 0.29 -0.5 459,246 - COO64_RS14485 0.30 -0.2 459,246 - COO64_RS14485 0.30 +0.8 459,246 - COO64_RS14485 0.30 +0.6 459,246 - COO64_RS14485 0.30 +0.1 459,246 - COO64_RS14485 0.30 -0.5 459,345 + COO64_RS14485 0.44 -0.7 459,345 + COO64_RS14485 0.44 -1.7 459,529 + COO64_RS14485 0.70 +0.2 459,758 + -1.0 459,758 + -0.7 459,759 - -0.1 459,759 - +0.1 459,759 - +0.7 459,792 + +0.3 459,792 + +0.2 459,793 - -0.4 459,793 - -0.6 460,155 + COO64_RS14490 0.40 -1.3 460,156 - COO64_RS14490 0.40 +0.5 460,156 - COO64_RS14490 0.40 +0.3 460,156 - COO64_RS14490 0.40 -0.1 460,156 - COO64_RS14490 0.40 -0.7 460,216 - COO64_RS14490 0.48 -0.6 460,228 - COO64_RS14490 0.49 +0.4 460,313 + COO64_RS14490 0.60 -0.1 460,313 + COO64_RS14490 0.60 -0.4 460,446 + COO64_RS14490 0.76 -0.3 460,493 + COO64_RS14490 0.82 +1.1 460,493 + COO64_RS14490 0.82 -0.2 460,494 - COO64_RS14490 0.82 -0.3 460,494 - COO64_RS14490 0.82 +0.5 460,494 - COO64_RS14490 0.82 -0.1 460,527 + COO64_RS14490 0.86 +2.1 460,527 + COO64_RS14490 0.86 +0.5 460,527 + COO64_RS14490 0.86 -0.2 460,527 + COO64_RS14490 0.86 -0.4 460,527 + COO64_RS14490 0.86 -0.8 460,527 + COO64_RS14490 0.86 -0.7 460,528 - COO64_RS14490 0.86 -0.3 460,528 - COO64_RS14490 0.86 +0.1 460,528 - COO64_RS14490 0.86 +0.4 460,549 - COO64_RS14490 0.88 -0.8 460,618 + +0.4 460,619 - -0.1 460,619 - -0.5 460,642 + +0.6 460,642 + -0.7 460,642 + +1.1 460,642 + -0.4 460,642 + -0.8 460,642 + +0.3 460,642 + +0.0 460,642 + -0.1 460,642 + +0.5 460,642 + +0.5 460,643 - -0.6 460,643 - +0.3 460,643 - +0.5 460,643 - -0.4 460,643 - +0.3 460,643 - +0.3 460,674 + -1.2 460,675 - +0.8 460,675 - -0.4 460,675 - -0.8
Or see this region's nucleotide sequence