Experiment: Pustulan (b-1-6 glucan) 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_04690 and BACOVA_04691 overlap by 4 nucleotides BACOVA_04691 and BACOVA_04692 overlap by 10 nucleotides BACOVA_04692 and BACOVA_04693 overlap by 17 nucleotides BACOVA_04693 and BACOVA_04694 are separated by 88 nucleotides BACOVA_04694 and BACOVA_04695 overlap by 26 nucleotides
BACOVA_04690: BACOVA_04690 - hypothetical protein, at 59,326 to 59,796
_04690
BACOVA_04691: BACOVA_04691 - hypothetical protein, at 59,793 to 60,194
_04691
BACOVA_04692: BACOVA_04692 - hypothetical protein, at 60,185 to 60,775
_04692
BACOVA_04693: BACOVA_04693 - hypothetical protein, at 60,759 to 61,610
_04693
BACOVA_04694: BACOVA_04694 - hypothetical protein, at 61,699 to 61,854
_04694
BACOVA_04695: BACOVA_04695 - putative plasmid recombination enzyme, at 61,829 to 63,286
_04695
Position (kb)
60
61
62 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 59.806 kb on + strand at 59.824 kb on - strand at 59.824 kb on - strand at 59.885 kb on + strand, within BACOVA_04691 at 59.885 kb on + strand, within BACOVA_04691 at 59.885 kb on + strand, within BACOVA_04691 at 59.963 kb on - strand, within BACOVA_04691 at 59.963 kb on - strand, within BACOVA_04691 at 60.041 kb on + strand, within BACOVA_04691 at 60.076 kb on - strand, within BACOVA_04691 at 60.180 kb on + strand at 60.185 kb on - strand at 60.185 kb on - strand at 60.222 kb on + strand at 60.281 kb on + strand, within BACOVA_04692 at 60.282 kb on - strand, within BACOVA_04692 at 60.320 kb on + strand, within BACOVA_04692 at 60.320 kb on + strand, within BACOVA_04692 at 60.321 kb on - strand, within BACOVA_04692 at 60.321 kb on - strand, within BACOVA_04692 at 60.321 kb on - strand, within BACOVA_04692 at 60.331 kb on - strand, within BACOVA_04692 at 60.339 kb on + strand, within BACOVA_04692 at 60.340 kb on - strand, within BACOVA_04692 at 60.359 kb on + strand, within BACOVA_04692 at 60.360 kb on - strand, within BACOVA_04692 at 60.363 kb on + strand, within BACOVA_04692 at 60.363 kb on + strand, within BACOVA_04692 at 60.364 kb on - strand, within BACOVA_04692 at 60.364 kb on - strand, within BACOVA_04692 at 60.379 kb on + strand, within BACOVA_04692 at 60.380 kb on - strand, within BACOVA_04692 at 60.400 kb on - strand, within BACOVA_04692 at 60.536 kb on + strand, within BACOVA_04692 at 60.537 kb on - strand, within BACOVA_04692 at 60.557 kb on - strand, within BACOVA_04692 at 60.560 kb on + strand, within BACOVA_04692 at 60.730 kb on - strand at 60.740 kb on + strand at 60.741 kb on - strand at 60.741 kb on - strand at 60.785 kb on - strand at 60.814 kb on + strand at 60.823 kb on - strand at 60.868 kb on - strand, within BACOVA_04693 at 60.930 kb on + strand, within BACOVA_04693 at 61.091 kb on - strand, within BACOVA_04693 at 61.091 kb on - strand, within BACOVA_04693 at 61.163 kb on - strand, within BACOVA_04693 at 61.163 kb on - strand, within BACOVA_04693 at 61.228 kb on + strand, within BACOVA_04693 at 61.681 kb on + strand at 61.683 kb on + strand at 61.684 kb on - strand at 61.736 kb on + strand, within BACOVA_04694 at 61.936 kb on + strand at 61.942 kb on + strand at 61.952 kb on + strand at 61.952 kb on + strand at 61.952 kb on + strand at 62.005 kb on - strand, within BACOVA_04695 at 62.005 kb on - strand, within BACOVA_04695 at 62.005 kb on - strand, within BACOVA_04695 at 62.053 kb on - strand, within BACOVA_04695 at 62.206 kb on + strand, within BACOVA_04695 at 62.207 kb on - strand, within BACOVA_04695 at 62.213 kb on - strand, within BACOVA_04695 at 62.256 kb on - strand, within BACOVA_04695 at 62.256 kb on - strand, within BACOVA_04695 at 62.277 kb on - strand, within BACOVA_04695 at 62.437 kb on + strand, within BACOVA_04695 at 62.462 kb on - strand, within BACOVA_04695 at 62.493 kb on + strand, within BACOVA_04695
Per-strain Table
Position Strand Gene LocusTag Fraction Pustulan (b-1-6 glucan) 2.5 mg/mL (C) remove 59,806 + -0.8 59,824 - +0.1 59,824 - -0.4 59,885 + BACOVA_04691 0.23 +2.5 59,885 + BACOVA_04691 0.23 -1.1 59,885 + BACOVA_04691 0.23 +1.4 59,963 - BACOVA_04691 0.42 +0.1 59,963 - BACOVA_04691 0.42 -1.3 60,041 + BACOVA_04691 0.62 +0.4 60,076 - BACOVA_04691 0.70 -0.5 60,180 + -0.0 60,185 - -0.6 60,185 - -0.8 60,222 + -1.7 60,281 + BACOVA_04692 0.16 +0.5 60,282 - BACOVA_04692 0.16 -1.0 60,320 + BACOVA_04692 0.23 -0.1 60,320 + BACOVA_04692 0.23 +0.3 60,321 - BACOVA_04692 0.23 -0.1 60,321 - BACOVA_04692 0.23 -1.4 60,321 - BACOVA_04692 0.23 +0.4 60,331 - BACOVA_04692 0.25 -0.0 60,339 + BACOVA_04692 0.26 +0.5 60,340 - BACOVA_04692 0.26 +1.0 60,359 + BACOVA_04692 0.29 -0.3 60,360 - BACOVA_04692 0.30 -2.4 60,363 + BACOVA_04692 0.30 -0.6 60,363 + BACOVA_04692 0.30 -0.5 60,364 - BACOVA_04692 0.30 -0.0 60,364 - BACOVA_04692 0.30 -0.3 60,379 + BACOVA_04692 0.33 -1.1 60,380 - BACOVA_04692 0.33 -0.0 60,400 - BACOVA_04692 0.36 -1.1 60,536 + BACOVA_04692 0.59 +0.8 60,537 - BACOVA_04692 0.60 -1.4 60,557 - BACOVA_04692 0.63 -0.4 60,560 + BACOVA_04692 0.63 +0.7 60,730 - -1.5 60,740 + +0.3 60,741 - +0.9 60,741 - -0.0 60,785 - -0.1 60,814 + +0.7 60,823 - -1.6 60,868 - BACOVA_04693 0.13 +0.1 60,930 + BACOVA_04693 0.20 -0.2 61,091 - BACOVA_04693 0.39 -1.0 61,091 - BACOVA_04693 0.39 -1.3 61,163 - BACOVA_04693 0.47 -1.1 61,163 - BACOVA_04693 0.47 -0.3 61,228 + BACOVA_04693 0.55 +1.3 61,681 + -0.1 61,683 + +0.4 61,684 - -2.0 61,736 + BACOVA_04694 0.24 +1.0 61,936 + +1.3 61,942 + +0.3 61,952 + -0.8 61,952 + -0.1 61,952 + +0.7 62,005 - BACOVA_04695 0.12 +0.7 62,005 - BACOVA_04695 0.12 +0.6 62,005 - BACOVA_04695 0.12 -1.0 62,053 - BACOVA_04695 0.15 -2.0 62,206 + BACOVA_04695 0.26 +0.9 62,207 - BACOVA_04695 0.26 +0.1 62,213 - BACOVA_04695 0.26 -0.3 62,256 - BACOVA_04695 0.29 -1.7 62,256 - BACOVA_04695 0.29 -1.2 62,277 - BACOVA_04695 0.31 -1.0 62,437 + BACOVA_04695 0.42 +0.0 62,462 - BACOVA_04695 0.43 +0.5 62,493 + BACOVA_04695 0.46 -1.5
Or see this region's nucleotide sequence