Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dAP2 and OKGIIK_13375 are separated by 148 nucleotides OKGIIK_13375 and gstA are separated by 77 nucleotides gstA and lysR are separated by 9 nucleotides
OKGIIK_13370: dAP2 - S9 family peptidase, at 2,887,872 to 2,890,208
dAP2
OKGIIK_13375: OKGIIK_13375 - Acyl-CoA hydrolase, at 2,890,357 to 2,890,794
_13375
OKGIIK_13380: gstA - glutathione S-transferase family protein, at 2,890,872 to 2,891,624
gstA
OKGIIK_13385: lysR - LysR family transcriptional regulator, at 2,891,634 to 2,892,533
lysR
Position (kb)
2890
2891
2892 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2890.009 kb on + strand at 2890.010 kb on - strand at 2890.037 kb on + strand at 2890.078 kb on + strand at 2890.078 kb on + strand at 2890.079 kb on - strand at 2890.079 kb on - strand at 2890.079 kb on - strand at 2890.105 kb on + strand at 2890.106 kb on - strand at 2890.800 kb on + strand at 2890.800 kb on + strand at 2890.801 kb on - strand at 2890.902 kb on + strand at 2890.902 kb on + strand at 2890.903 kb on - strand at 2890.903 kb on - strand at 2890.903 kb on - strand at 2890.903 kb on - strand at 2890.914 kb on + strand at 2890.914 kb on + strand at 2890.914 kb on + strand at 2890.915 kb on - strand at 2890.915 kb on - strand at 2890.915 kb on - strand at 2890.915 kb on - strand at 2890.915 kb on - strand at 2890.927 kb on + strand at 2890.927 kb on + strand at 2890.927 kb on + strand at 2890.927 kb on + strand at 2890.928 kb on - strand at 2890.928 kb on - strand at 2890.928 kb on - strand at 2890.928 kb on - strand at 2890.959 kb on + strand, within gstA at 2890.959 kb on + strand, within gstA at 2890.959 kb on + strand, within gstA at 2890.960 kb on - strand, within gstA at 2890.960 kb on - strand, within gstA at 2890.960 kb on - strand, within gstA at 2891.086 kb on - strand, within gstA at 2891.086 kb on - strand, within gstA at 2891.086 kb on - strand, within gstA at 2891.118 kb on + strand, within gstA at 2891.167 kb on - strand, within gstA at 2891.167 kb on - strand, within gstA at 2891.167 kb on - strand, within gstA at 2891.307 kb on + strand, within gstA at 2891.307 kb on + strand, within gstA at 2891.307 kb on + strand, within gstA at 2891.308 kb on - strand, within gstA at 2891.409 kb on + strand, within gstA at 2891.496 kb on + strand, within gstA at 2891.713 kb on + strand at 2891.848 kb on + strand, within lysR at 2891.848 kb on + strand, within lysR at 2891.848 kb on + strand, within lysR at 2891.849 kb on - strand, within lysR at 2892.023 kb on - strand, within lysR at 2892.023 kb on - strand, within lysR at 2892.337 kb on + strand, within lysR at 2892.338 kb on - strand, within lysR at 2892.338 kb on - strand, within lysR at 2892.338 kb on - strand, within lysR at 2892.340 kb on + strand, within lysR at 2892.340 kb on + strand, within lysR at 2892.340 kb on + strand, within lysR at 2892.340 kb on + strand, within lysR at 2892.340 kb on + strand, within lysR at 2892.340 kb on + strand, within lysR at 2892.341 kb on - strand, within lysR at 2892.341 kb on - strand, within lysR at 2892.341 kb on - strand, within lysR at 2892.341 kb on - strand, within lysR at 2892.377 kb on + strand, within lysR at 2892.503 kb on + strand at 2892.503 kb on + strand at 2892.503 kb on + strand at 2892.504 kb on - strand at 2892.504 kb on - strand at 2892.505 kb on + strand at 2892.505 kb on + strand at 2892.505 kb on + strand at 2892.505 kb on + strand at 2892.506 kb on - strand at 2892.506 kb on - strand at 2892.506 kb on - strand at 2892.569 kb on + strand at 2892.569 kb on + strand at 2892.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 2,890,009 + -0.4 2,890,010 - -0.2 2,890,037 + -0.8 2,890,078 + -1.0 2,890,078 + -0.4 2,890,079 - -1.9 2,890,079 - -0.4 2,890,079 - -1.4 2,890,105 + -0.4 2,890,106 - -0.1 2,890,800 + +0.6 2,890,800 + +0.5 2,890,801 - -0.5 2,890,902 + +0.4 2,890,902 + +0.0 2,890,903 - -0.3 2,890,903 - -0.2 2,890,903 - -0.7 2,890,903 - -1.3 2,890,914 + +0.9 2,890,914 + +0.9 2,890,914 + -0.1 2,890,915 - +1.7 2,890,915 - +0.0 2,890,915 - -0.6 2,890,915 - -0.2 2,890,915 - -0.5 2,890,927 + -0.0 2,890,927 + -1.5 2,890,927 + -1.1 2,890,927 + -1.9 2,890,928 - -1.9 2,890,928 - -1.7 2,890,928 - +1.1 2,890,928 - -0.3 2,890,959 + gstA OKGIIK_13380 0.12 -0.3 2,890,959 + gstA OKGIIK_13380 0.12 -0.8 2,890,959 + gstA OKGIIK_13380 0.12 -1.0 2,890,960 - gstA OKGIIK_13380 0.12 +0.3 2,890,960 - gstA OKGIIK_13380 0.12 -0.8 2,890,960 - gstA OKGIIK_13380 0.12 +1.9 2,891,086 - gstA OKGIIK_13380 0.28 -0.0 2,891,086 - gstA OKGIIK_13380 0.28 +0.9 2,891,086 - gstA OKGIIK_13380 0.28 +1.7 2,891,118 + gstA OKGIIK_13380 0.33 +0.2 2,891,167 - gstA OKGIIK_13380 0.39 -1.4 2,891,167 - gstA OKGIIK_13380 0.39 +0.4 2,891,167 - gstA OKGIIK_13380 0.39 +0.4 2,891,307 + gstA OKGIIK_13380 0.58 +0.4 2,891,307 + gstA OKGIIK_13380 0.58 -1.3 2,891,307 + gstA OKGIIK_13380 0.58 +0.3 2,891,308 - gstA OKGIIK_13380 0.58 -1.1 2,891,409 + gstA OKGIIK_13380 0.71 -3.6 2,891,496 + gstA OKGIIK_13380 0.83 -0.1 2,891,713 + +0.1 2,891,848 + lysR OKGIIK_13385 0.24 -0.1 2,891,848 + lysR OKGIIK_13385 0.24 +0.9 2,891,848 + lysR OKGIIK_13385 0.24 -0.2 2,891,849 - lysR OKGIIK_13385 0.24 -1.3 2,892,023 - lysR OKGIIK_13385 0.43 +0.4 2,892,023 - lysR OKGIIK_13385 0.43 -1.2 2,892,337 + lysR OKGIIK_13385 0.78 +0.4 2,892,338 - lysR OKGIIK_13385 0.78 -0.4 2,892,338 - lysR OKGIIK_13385 0.78 -1.1 2,892,338 - lysR OKGIIK_13385 0.78 +0.1 2,892,340 + lysR OKGIIK_13385 0.78 -0.1 2,892,340 + lysR OKGIIK_13385 0.78 -2.2 2,892,340 + lysR OKGIIK_13385 0.78 -2.1 2,892,340 + lysR OKGIIK_13385 0.78 -0.7 2,892,340 + lysR OKGIIK_13385 0.78 -0.4 2,892,340 + lysR OKGIIK_13385 0.78 +0.2 2,892,341 - lysR OKGIIK_13385 0.79 -0.7 2,892,341 - lysR OKGIIK_13385 0.79 -0.3 2,892,341 - lysR OKGIIK_13385 0.79 -0.5 2,892,341 - lysR OKGIIK_13385 0.79 -2.1 2,892,377 + lysR OKGIIK_13385 0.83 +0.4 2,892,503 + -0.1 2,892,503 + +0.0 2,892,503 + -1.3 2,892,504 - -1.1 2,892,504 - -0.7 2,892,505 + +0.8 2,892,505 + -1.2 2,892,505 + +0.1 2,892,505 + -0.1 2,892,506 - +0.4 2,892,506 - -0.2 2,892,506 - -2.0 2,892,569 + +0.9 2,892,569 + +0.9 2,892,570 - -1.1
Or see this region's nucleotide sequence