Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prs and Gln_trna are separated by 33 nucleotides Gln_trna and ispE are separated by 63 nucleotides ispE and lolB are separated by 50 nucleotides lolB and OKGIIK_10600 overlap by 4 nucleotides
OKGIIK_10580: prs - ribose-phosphate diphosphokinase, at 2,270,475 to 2,271,434
prs
OKGIIK_10585: Gln_trna - tRNA-Gln, at 2,271,468 to 2,271,544
_trna
OKGIIK_10590: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 2,271,608 to 2,273,008
ispE
OKGIIK_10595: lolB - lipoprotein insertase outer membrane protein LolB, at 2,273,059 to 2,273,670
lolB
OKGIIK_10600: OKGIIK_10600 - Beta-barrel assembly-enhancing protease, at 2,273,667 to 2,275,424
_10600
Position (kb)
2271
2272
2273
2274 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.621 kb on - strand at 2271.621 kb on - strand at 2271.621 kb on - strand at 2272.469 kb on + strand, within ispE at 2272.470 kb on - strand, within ispE at 2272.470 kb on - strand, within ispE at 2272.618 kb on - strand, within ispE at 2272.618 kb on - strand, within ispE at 2272.618 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.738 kb on - strand, within ispE at 2272.738 kb on - strand, within ispE at 2272.738 kb on - strand, within ispE at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.053 kb on - strand at 2273.053 kb on - strand at 2273.053 kb on - strand at 2273.872 kb on + strand, within OKGIIK_10600 at 2273.873 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 2,271,442 - +0.5 2,271,442 - -2.0 2,271,442 - -2.4 2,271,442 - -0.3 2,271,550 - +0.7 2,271,550 - -0.5 2,271,550 - +0.2 2,271,550 - -0.4 2,271,550 - -0.3 2,271,550 - +0.3 2,271,550 - -2.3 2,271,550 - -0.4 2,271,550 - +0.0 2,271,550 - -1.1 2,271,550 - -0.0 2,271,550 - +0.0 2,271,550 - +1.4 2,271,550 - -0.8 2,271,550 - +2.6 2,271,550 - -1.1 2,271,550 - -0.1 2,271,550 - -0.9 2,271,550 - +0.8 2,271,550 - -0.3 2,271,550 - +0.4 2,271,550 - +1.2 2,271,550 - -0.5 2,271,550 - -1.4 2,271,550 - +1.5 2,271,550 - -0.5 2,271,550 - -0.8 2,271,550 - -3.2 2,271,550 - -0.2 2,271,550 - -0.8 2,271,550 - +0.6 2,271,550 - +0.7 2,271,550 - +0.6 2,271,550 - -0.3 2,271,550 - +0.2 2,271,550 - +1.0 2,271,550 - -1.5 2,271,550 - +0.9 2,271,550 - +0.0 2,271,550 - +0.8 2,271,550 - -1.5 2,271,550 - +1.4 2,271,550 - +0.7 2,271,550 - -0.1 2,271,550 - -1.8 2,271,550 - -0.5 2,271,550 - -2.3 2,271,550 - -1.5 2,271,550 - -1.5 2,271,550 - -0.8 2,271,550 - +0.0 2,271,574 - +1.7 2,271,574 - -0.4 2,271,574 - -0.0 2,271,574 - +1.5 2,271,574 - +0.1 2,271,574 - +2.0 2,271,574 - -1.0 2,271,574 - -0.4 2,271,574 - +1.0 2,271,574 - +0.5 2,271,574 - +0.7 2,271,574 - +0.8 2,271,574 - -0.7 2,271,574 - -1.5 2,271,574 - +1.7 2,271,574 - -0.7 2,271,574 - -0.1 2,271,574 - -0.9 2,271,574 - -1.3 2,271,574 - +1.5 2,271,574 - -1.7 2,271,574 - +0.2 2,271,574 - -0.4 2,271,574 - -2.3 2,271,621 - -0.4 2,271,621 - -1.8 2,271,621 - -1.5 2,272,469 + ispE OKGIIK_10590 0.61 +0.8 2,272,470 - ispE OKGIIK_10590 0.62 -1.7 2,272,470 - ispE OKGIIK_10590 0.62 -0.5 2,272,618 - ispE OKGIIK_10590 0.72 -3.0 2,272,618 - ispE OKGIIK_10590 0.72 -1.4 2,272,618 - ispE OKGIIK_10590 0.72 -0.0 2,272,638 - ispE OKGIIK_10590 0.74 -0.8 2,272,638 - ispE OKGIIK_10590 0.74 -0.9 2,272,638 - ispE OKGIIK_10590 0.74 -0.5 2,272,638 - ispE OKGIIK_10590 0.74 -1.3 2,272,638 - ispE OKGIIK_10590 0.74 -0.4 2,272,638 - ispE OKGIIK_10590 0.74 +1.2 2,272,638 - ispE OKGIIK_10590 0.74 -1.0 2,272,638 - ispE OKGIIK_10590 0.74 -1.0 2,272,638 - ispE OKGIIK_10590 0.74 -1.8 2,272,738 - ispE OKGIIK_10590 0.81 -0.2 2,272,738 - ispE OKGIIK_10590 0.81 -0.4 2,272,738 - ispE OKGIIK_10590 0.81 -1.5 2,272,903 - +0.0 2,272,903 - -1.5 2,272,903 - -2.0 2,272,903 - +0.3 2,272,903 - -1.0 2,272,903 - -1.5 2,273,052 + +0.0 2,273,052 + -1.0 2,273,052 + +0.2 2,273,052 + -1.3 2,273,053 - -0.5 2,273,053 - +0.2 2,273,053 - +1.5 2,273,872 + OKGIIK_10600 0.12 -0.4 2,273,873 - OKGIIK_10600 0.12 -0.5 2,273,987 - OKGIIK_10600 0.18 -1.5 2,273,987 - OKGIIK_10600 0.18 -1.7 2,273,987 - OKGIIK_10600 0.18 -1.3 2,273,987 - OKGIIK_10600 0.18 -0.8 2,273,987 - OKGIIK_10600 0.18 -1.0 2,273,987 - OKGIIK_10600 0.18 -1.0 2,273,987 - OKGIIK_10600 0.18 +0.5 2,273,987 - OKGIIK_10600 0.18 -0.8
Or see this region's nucleotide sequence