Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaK and grpE are separated by 134 nucleotides grpE and hrcA are separated by 81 nucleotides hrcA and recN are separated by 128 nucleotides
OKGIIK_07325: dnaK - molecular chaperone DnaK, at 1,621,226 to 1,623,142
dnaK
OKGIIK_07330: grpE - nucleotide exchange factor GrpE, at 1,623,277 to 1,623,807
grpE
OKGIIK_07335: hrcA - heat-inducible transcriptional repressor HrcA, at 1,623,889 to 1,624,959
hrcA
OKGIIK_07340: recN - DNA repair protein RecN, at 1,625,088 to 1,626,761
recN
Position (kb)
1623
1624
1625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1623.890 kb on + strand at 1623.891 kb on - strand at 1623.891 kb on - strand at 1623.891 kb on - strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.892 kb on + strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.893 kb on - strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.968 kb on + strand at 1623.969 kb on - strand at 1623.969 kb on - strand at 1623.969 kb on - strand at 1623.969 kb on - strand at 1624.009 kb on + strand, within hrcA at 1624.010 kb on - strand, within hrcA at 1624.010 kb on - strand, within hrcA at 1624.028 kb on + strand, within hrcA at 1624.028 kb on + strand, within hrcA at 1624.028 kb on + strand, within hrcA at 1624.028 kb on + strand, within hrcA at 1624.028 kb on + strand, within hrcA at 1624.029 kb on - strand, within hrcA at 1624.029 kb on - strand, within hrcA at 1624.029 kb on - strand, within hrcA at 1624.029 kb on - strand, within hrcA at 1624.202 kb on + strand, within hrcA at 1624.202 kb on + strand, within hrcA at 1624.202 kb on + strand, within hrcA at 1624.202 kb on + strand, within hrcA at 1624.202 kb on + strand, within hrcA at 1624.202 kb on + strand, within hrcA at 1624.203 kb on - strand, within hrcA at 1624.203 kb on - strand, within hrcA at 1624.203 kb on - strand, within hrcA at 1624.203 kb on - strand, within hrcA at 1624.303 kb on + strand, within hrcA at 1624.303 kb on + strand, within hrcA at 1624.304 kb on - strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.400 kb on + strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.401 kb on - strand, within hrcA at 1624.857 kb on + strand at 1624.857 kb on + strand at 1624.857 kb on + strand at 1624.857 kb on + strand at 1624.857 kb on + strand at 1624.857 kb on + strand at 1624.857 kb on + strand at 1624.858 kb on - strand at 1624.858 kb on - strand at 1624.858 kb on - strand at 1624.858 kb on - strand at 1624.889 kb on + strand at 1624.889 kb on + strand at 1624.889 kb on + strand at 1624.889 kb on + strand at 1624.889 kb on + strand at 1624.889 kb on + strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.890 kb on - strand at 1624.967 kb on + strand at 1624.967 kb on + strand at 1624.967 kb on + strand at 1624.967 kb on + strand at 1624.967 kb on + strand at 1624.967 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.993 kb on + strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1624.994 kb on - strand at 1625.056 kb on + strand at 1625.057 kb on - strand at 1625.057 kb on - strand at 1625.057 kb on - strand at 1625.057 kb on - strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.103 kb on + strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.104 kb on - strand at 1625.532 kb on + strand, within recN at 1625.532 kb on + strand, within recN at 1625.532 kb on + strand, within recN at 1625.532 kb on + strand, within recN at 1625.532 kb on + strand, within recN at 1625.532 kb on + strand, within recN at 1625.532 kb on + strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.533 kb on - strand, within recN at 1625.943 kb on + strand, within recN at 1625.943 kb on + strand, within recN at 1625.943 kb on + strand, within recN at 1625.944 kb on - strand, within recN at 1625.944 kb on - strand, within recN
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 1,623,890 + +0.8 1,623,891 - -1.3 1,623,891 - +1.6 1,623,891 - +0.6 1,623,892 + +0.1 1,623,892 + +0.3 1,623,892 + +0.2 1,623,892 + +0.6 1,623,892 + +0.5 1,623,892 + -0.2 1,623,892 + -0.9 1,623,892 + -1.3 1,623,892 + +0.9 1,623,892 + +0.5 1,623,892 + -0.5 1,623,892 + -0.3 1,623,892 + +1.1 1,623,892 + -0.5 1,623,892 + -0.5 1,623,892 + -0.0 1,623,892 + -1.1 1,623,893 - +1.8 1,623,893 - -2.1 1,623,893 - -0.4 1,623,893 - -0.4 1,623,893 - -0.6 1,623,893 - +0.2 1,623,893 - -1.5 1,623,893 - +1.1 1,623,893 - -1.1 1,623,893 - +0.1 1,623,893 - -0.9 1,623,893 - +0.1 1,623,893 - +1.1 1,623,968 + -0.3 1,623,968 + +1.8 1,623,968 + +1.1 1,623,968 + -0.9 1,623,968 + +1.1 1,623,968 + -1.1 1,623,968 + -0.8 1,623,968 + +1.1 1,623,968 + +0.3 1,623,968 + +0.7 1,623,968 + +0.1 1,623,968 + +0.6 1,623,968 + +1.6 1,623,969 - +2.6 1,623,969 - +1.1 1,623,969 - -0.9 1,623,969 - -1.3 1,624,009 + hrcA OKGIIK_07335 0.11 +0.4 1,624,010 - hrcA OKGIIK_07335 0.11 -1.3 1,624,010 - hrcA OKGIIK_07335 0.11 -0.9 1,624,028 + hrcA OKGIIK_07335 0.13 +1.1 1,624,028 + hrcA OKGIIK_07335 0.13 +0.3 1,624,028 + hrcA OKGIIK_07335 0.13 +0.8 1,624,028 + hrcA OKGIIK_07335 0.13 +0.1 1,624,028 + hrcA OKGIIK_07335 0.13 +2.5 1,624,029 - hrcA OKGIIK_07335 0.13 -0.8 1,624,029 - hrcA OKGIIK_07335 0.13 +0.2 1,624,029 - hrcA OKGIIK_07335 0.13 +2.1 1,624,029 - hrcA OKGIIK_07335 0.13 -1.5 1,624,202 + hrcA OKGIIK_07335 0.29 +1.8 1,624,202 + hrcA OKGIIK_07335 0.29 -0.3 1,624,202 + hrcA OKGIIK_07335 0.29 +0.2 1,624,202 + hrcA OKGIIK_07335 0.29 +0.1 1,624,202 + hrcA OKGIIK_07335 0.29 +0.1 1,624,202 + hrcA OKGIIK_07335 0.29 +0.3 1,624,203 - hrcA OKGIIK_07335 0.29 +3.4 1,624,203 - hrcA OKGIIK_07335 0.29 +2.2 1,624,203 - hrcA OKGIIK_07335 0.29 +1.3 1,624,203 - hrcA OKGIIK_07335 0.29 -0.5 1,624,303 + hrcA OKGIIK_07335 0.39 -0.8 1,624,303 + hrcA OKGIIK_07335 0.39 -1.4 1,624,304 - hrcA OKGIIK_07335 0.39 -1.3 1,624,400 + hrcA OKGIIK_07335 0.48 +0.7 1,624,400 + hrcA OKGIIK_07335 0.48 -0.2 1,624,400 + hrcA OKGIIK_07335 0.48 +1.0 1,624,400 + hrcA OKGIIK_07335 0.48 -3.0 1,624,400 + hrcA OKGIIK_07335 0.48 +0.2 1,624,400 + hrcA OKGIIK_07335 0.48 +0.2 1,624,400 + hrcA OKGIIK_07335 0.48 +0.8 1,624,400 + hrcA OKGIIK_07335 0.48 -0.4 1,624,400 + hrcA OKGIIK_07335 0.48 -1.9 1,624,400 + hrcA OKGIIK_07335 0.48 +0.2 1,624,400 + hrcA OKGIIK_07335 0.48 -0.9 1,624,401 - hrcA OKGIIK_07335 0.48 -0.3 1,624,401 - hrcA OKGIIK_07335 0.48 -2.3 1,624,401 - hrcA OKGIIK_07335 0.48 +0.4 1,624,401 - hrcA OKGIIK_07335 0.48 +0.4 1,624,401 - hrcA OKGIIK_07335 0.48 -0.5 1,624,401 - hrcA OKGIIK_07335 0.48 +0.3 1,624,401 - hrcA OKGIIK_07335 0.48 -0.8 1,624,401 - hrcA OKGIIK_07335 0.48 -0.9 1,624,401 - hrcA OKGIIK_07335 0.48 +0.1 1,624,401 - hrcA OKGIIK_07335 0.48 +0.1 1,624,401 - hrcA OKGIIK_07335 0.48 -0.8 1,624,857 + -1.5 1,624,857 + +0.1 1,624,857 + +1.5 1,624,857 + +0.1 1,624,857 + -0.9 1,624,857 + +0.1 1,624,857 + +1.8 1,624,858 - +0.1 1,624,858 - +0.6 1,624,858 - +0.5 1,624,858 - -0.8 1,624,889 + +0.1 1,624,889 + +0.8 1,624,889 + +0.3 1,624,889 + -0.1 1,624,889 + +1.1 1,624,889 + +0.5 1,624,890 - -0.5 1,624,890 - -0.5 1,624,890 - +0.5 1,624,890 - +1.1 1,624,890 - -0.9 1,624,890 - -0.3 1,624,890 - +0.6 1,624,890 - -0.6 1,624,967 + -0.2 1,624,967 + +3.6 1,624,967 + -0.7 1,624,967 + +0.3 1,624,967 + -0.5 1,624,967 + -1.0 1,624,993 + +0.1 1,624,993 + +0.5 1,624,993 + +0.7 1,624,993 + +1.6 1,624,993 + +0.8 1,624,993 + -0.4 1,624,993 + +0.1 1,624,993 + +1.0 1,624,993 + +0.4 1,624,993 + +0.6 1,624,993 + -1.8 1,624,993 + -0.3 1,624,993 + +2.1 1,624,993 + +0.6 1,624,993 + -1.4 1,624,993 + -2.5 1,624,993 + +1.5 1,624,994 - -0.2 1,624,994 - +0.7 1,624,994 - -0.9 1,624,994 - -0.5 1,624,994 - -0.8 1,624,994 - -0.6 1,624,994 - +1.0 1,624,994 - -1.3 1,624,994 - +1.3 1,624,994 - -1.2 1,624,994 - -2.9 1,625,056 + +0.1 1,625,057 - -2.1 1,625,057 - -0.3 1,625,057 - +0.8 1,625,057 - +0.6 1,625,103 + +0.1 1,625,103 + -1.1 1,625,103 + +1.9 1,625,103 + -1.8 1,625,103 + -1.5 1,625,103 + +1.8 1,625,103 + -0.5 1,625,103 + -1.5 1,625,103 + -0.5 1,625,103 + -1.1 1,625,103 + -0.4 1,625,104 - +2.1 1,625,104 - -1.1 1,625,104 - -1.3 1,625,104 - -1.8 1,625,104 - +1.5 1,625,104 - -0.5 1,625,104 - +1.1 1,625,104 - +0.3 1,625,104 - -2.0 1,625,104 - +0.9 1,625,104 - +0.0 1,625,104 - -0.9 1,625,104 - -0.2 1,625,104 - -0.1 1,625,104 - -1.8 1,625,104 - -1.1 1,625,104 - +0.5 1,625,104 - +1.1 1,625,532 + recN OKGIIK_07340 0.27 -0.9 1,625,532 + recN OKGIIK_07340 0.27 -0.0 1,625,532 + recN OKGIIK_07340 0.27 +1.5 1,625,532 + recN OKGIIK_07340 0.27 +2.1 1,625,532 + recN OKGIIK_07340 0.27 +1.1 1,625,532 + recN OKGIIK_07340 0.27 -3.2 1,625,532 + recN OKGIIK_07340 0.27 -0.8 1,625,533 - recN OKGIIK_07340 0.27 +0.2 1,625,533 - recN OKGIIK_07340 0.27 -0.7 1,625,533 - recN OKGIIK_07340 0.27 +1.1 1,625,533 - recN OKGIIK_07340 0.27 -1.0 1,625,533 - recN OKGIIK_07340 0.27 -1.7 1,625,533 - recN OKGIIK_07340 0.27 +0.8 1,625,533 - recN OKGIIK_07340 0.27 -0.4 1,625,533 - recN OKGIIK_07340 0.27 +0.2 1,625,943 + recN OKGIIK_07340 0.51 -1.0 1,625,943 + recN OKGIIK_07340 0.51 -0.0 1,625,943 + recN OKGIIK_07340 0.51 -1.9 1,625,944 - recN OKGIIK_07340 0.51 +2.3 1,625,944 - recN OKGIIK_07340 0.51 +1.4
Or see this region's nucleotide sequence