Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and panB are separated by 55 nucleotides panB and panC are separated by 16 nucleotides panC and panD are separated by 102 nucleotides
OKGIIK_03875: pcnB - tRNA nucleotidyltransferase/poly(A) polymerase, at 851,396 to 852,745
pcnB
OKGIIK_03880: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 852,742 to 853,221
folK
OKGIIK_03885: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 853,277 to 854,107
panB
OKGIIK_03890: panC - pantoate--beta-alanine ligase, at 854,124 to 854,969
panC
OKGIIK_03895: panD - aspartate 1-decarboxylase, at 855,072 to 855,464
panD
Position (kb) 853
854
855 Strain fitness (log2 ratio) -2
-1
0
1
2 at 852.688 kb on + strand at 852.688 kb on + strand at 853.280 kb on + strand at 853.280 kb on + strand at 853.280 kb on + strand at 853.281 kb on - strand at 853.281 kb on - strand at 853.380 kb on - strand, within panB at 853.498 kb on + strand, within panB at 853.499 kb on - strand, within panB at 853.499 kb on - strand, within panB at 853.499 kb on - strand, within panB at 853.499 kb on - strand, within panB at 854.134 kb on + strand at 854.134 kb on + strand at 854.134 kb on + strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.325 kb on + strand, within panC at 854.325 kb on + strand, within panC at 854.326 kb on - strand, within panC at 854.418 kb on + strand, within panC at 854.418 kb on + strand, within panC at 854.698 kb on - strand, within panC at 854.980 kb on + strand at 854.981 kb on - strand at 854.995 kb on - strand at 855.065 kb on + strand at 855.065 kb on + strand at 855.066 kb on - strand at 855.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 852,688 + +1.3 852,688 + -0.9 853,280 + +0.8 853,280 + +0.5 853,280 + +1.3 853,281 - +0.1 853,281 - -0.9 853,380 - panB OKGIIK_03885 0.12 -0.9 853,498 + panB OKGIIK_03885 0.27 -0.9 853,499 - panB OKGIIK_03885 0.27 -1.3 853,499 - panB OKGIIK_03885 0.27 +1.7 853,499 - panB OKGIIK_03885 0.27 -0.9 853,499 - panB OKGIIK_03885 0.27 -2.1 854,134 + +0.3 854,134 + -0.9 854,134 + -0.3 854,135 - +0.2 854,135 - -0.3 854,135 - +1.2 854,135 - +0.8 854,135 - +0.7 854,325 + panC OKGIIK_03890 0.24 +0.1 854,325 + panC OKGIIK_03890 0.24 +0.1 854,326 - panC OKGIIK_03890 0.24 -0.2 854,418 + panC OKGIIK_03890 0.35 +0.1 854,418 + panC OKGIIK_03890 0.35 +0.8 854,698 - panC OKGIIK_03890 0.68 +0.3 854,980 + -0.7 854,981 - +2.1 854,995 - -1.0 855,065 + +1.3 855,065 + -1.5 855,066 - +1.2 855,066 - -0.5
Or see this region's nucleotide sequence