Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tcdA and fabG are separated by 96 nucleotides fabG and gluQRS are separated by 111 nucleotides gluQRS and htpX are separated by 126 nucleotides
OKGIIK_03630: tcdA - tRNA A37 threonylcarbamoyladenosine dehydratase, at 799,709 to 800,539
tcdA
OKGIIK_03635: fabG - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 800,636 to 801,376
fabG
OKGIIK_03640: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 801,488 to 802,354
gluQRS
OKGIIK_03645: htpX - protease HtpX, at 802,481 to 803,374
htpX
Position (kb) 801
802
803 Strain fitness (log2 ratio) -2
-1
0
1
2 at 800.538 kb on - strand at 800.538 kb on - strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.617 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.649 kb on + strand at 800.650 kb on - strand at 800.650 kb on - strand at 800.650 kb on - strand at 800.826 kb on + strand, within fabG at 800.826 kb on + strand, within fabG at 800.826 kb on + strand, within fabG at 800.827 kb on - strand, within fabG at 800.922 kb on + strand, within fabG at 800.922 kb on + strand, within fabG at 800.922 kb on + strand, within fabG at 800.922 kb on + strand, within fabG at 800.922 kb on + strand, within fabG at 800.922 kb on + strand, within fabG at 800.923 kb on - strand, within fabG at 800.923 kb on - strand, within fabG at 800.923 kb on - strand, within fabG at 800.937 kb on + strand, within fabG at 801.074 kb on - strand, within fabG at 801.075 kb on + strand, within fabG at 801.075 kb on + strand, within fabG at 801.075 kb on + strand, within fabG at 801.075 kb on + strand, within fabG at 801.075 kb on + strand, within fabG at 801.076 kb on - strand, within fabG at 801.076 kb on - strand, within fabG at 801.076 kb on - strand, within fabG at 801.076 kb on - strand, within fabG at 801.076 kb on - strand, within fabG at 801.076 kb on - strand, within fabG at 801.097 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.215 kb on + strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.216 kb on - strand, within fabG at 801.276 kb on + strand, within fabG at 801.276 kb on + strand, within fabG at 801.276 kb on + strand, within fabG at 801.276 kb on + strand, within fabG at 801.276 kb on + strand, within fabG at 801.277 kb on - strand, within fabG at 801.277 kb on - strand, within fabG at 801.277 kb on - strand, within fabG at 801.277 kb on - strand, within fabG at 801.312 kb on + strand at 801.313 kb on - strand at 801.364 kb on + strand at 801.364 kb on + strand at 801.364 kb on + strand at 801.365 kb on - strand at 801.365 kb on - strand at 801.365 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.480 kb on - strand at 801.481 kb on + strand at 801.482 kb on - strand at 801.482 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.717 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.613 kb on + strand, within htpX at 802.614 kb on - strand, within htpX at 802.614 kb on - strand, within htpX at 802.796 kb on + strand, within htpX at 802.796 kb on + strand, within htpX at 802.796 kb on + strand, within htpX at 802.797 kb on - strand, within htpX at 802.797 kb on - strand, within htpX at 803.078 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.252 kb on - strand, within htpX at 803.252 kb on - strand, within htpX at 803.252 kb on - strand, within htpX at 803.252 kb on - strand, within htpX
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 800,538 - -0.2 800,538 - +0.5 800,617 + -0.5 800,617 + +0.1 800,617 + -0.5 800,617 + -0.3 800,617 + +0.3 800,617 + -0.4 800,617 + -0.0 800,617 + +0.2 800,617 + -0.5 800,617 + +0.2 800,617 + +0.5 800,617 + -0.0 800,617 + -0.1 800,617 + -1.2 800,617 + +1.1 800,617 + +0.1 800,617 + +0.1 800,617 + -0.3 800,617 + +0.1 800,617 + -1.8 800,617 + +0.3 800,617 + +0.2 800,617 + -0.9 800,617 + -1.4 800,617 + +0.5 800,617 + +1.4 800,617 + -1.9 800,617 + -0.7 800,649 + -1.3 800,649 + +0.1 800,649 + -1.3 800,649 + -0.3 800,649 + -1.9 800,649 + -0.6 800,649 + -0.7 800,649 + -0.5 800,649 + +0.4 800,649 + -0.9 800,649 + -0.9 800,649 + -1.3 800,649 + -0.2 800,649 + -0.1 800,649 + -0.7 800,649 + +0.2 800,649 + -0.7 800,649 + -2.4 800,649 + -0.8 800,649 + -0.6 800,649 + -0.3 800,649 + -0.5 800,649 + +1.1 800,650 - +0.2 800,650 - +0.4 800,650 - -1.3 800,826 + fabG OKGIIK_03635 0.26 -0.4 800,826 + fabG OKGIIK_03635 0.26 +0.7 800,826 + fabG OKGIIK_03635 0.26 -0.5 800,827 - fabG OKGIIK_03635 0.26 -0.5 800,922 + fabG OKGIIK_03635 0.39 +0.5 800,922 + fabG OKGIIK_03635 0.39 +0.3 800,922 + fabG OKGIIK_03635 0.39 +0.1 800,922 + fabG OKGIIK_03635 0.39 -0.9 800,922 + fabG OKGIIK_03635 0.39 +0.7 800,922 + fabG OKGIIK_03635 0.39 -1.9 800,923 - fabG OKGIIK_03635 0.39 +0.5 800,923 - fabG OKGIIK_03635 0.39 +1.3 800,923 - fabG OKGIIK_03635 0.39 +0.3 800,937 + fabG OKGIIK_03635 0.41 +0.3 801,074 - fabG OKGIIK_03635 0.59 -0.9 801,075 + fabG OKGIIK_03635 0.59 -0.4 801,075 + fabG OKGIIK_03635 0.59 +0.9 801,075 + fabG OKGIIK_03635 0.59 -0.8 801,075 + fabG OKGIIK_03635 0.59 -2.3 801,075 + fabG OKGIIK_03635 0.59 +0.0 801,076 - fabG OKGIIK_03635 0.59 +0.2 801,076 - fabG OKGIIK_03635 0.59 -0.2 801,076 - fabG OKGIIK_03635 0.59 -1.4 801,076 - fabG OKGIIK_03635 0.59 -0.7 801,076 - fabG OKGIIK_03635 0.59 -1.3 801,076 - fabG OKGIIK_03635 0.59 -1.2 801,097 + fabG OKGIIK_03635 0.62 -1.3 801,215 + fabG OKGIIK_03635 0.78 +0.1 801,215 + fabG OKGIIK_03635 0.78 +0.1 801,215 + fabG OKGIIK_03635 0.78 +0.2 801,215 + fabG OKGIIK_03635 0.78 -0.1 801,215 + fabG OKGIIK_03635 0.78 +0.1 801,215 + fabG OKGIIK_03635 0.78 -0.5 801,215 + fabG OKGIIK_03635 0.78 +0.2 801,215 + fabG OKGIIK_03635 0.78 -1.5 801,215 + fabG OKGIIK_03635 0.78 +0.6 801,215 + fabG OKGIIK_03635 0.78 -0.2 801,215 + fabG OKGIIK_03635 0.78 -2.6 801,215 + fabG OKGIIK_03635 0.78 +0.1 801,215 + fabG OKGIIK_03635 0.78 -0.1 801,215 + fabG OKGIIK_03635 0.78 -0.1 801,215 + fabG OKGIIK_03635 0.78 -1.4 801,215 + fabG OKGIIK_03635 0.78 -1.3 801,215 + fabG OKGIIK_03635 0.78 -0.3 801,215 + fabG OKGIIK_03635 0.78 +0.1 801,215 + fabG OKGIIK_03635 0.78 -1.4 801,216 - fabG OKGIIK_03635 0.78 -1.9 801,216 - fabG OKGIIK_03635 0.78 +0.2 801,216 - fabG OKGIIK_03635 0.78 -1.5 801,216 - fabG OKGIIK_03635 0.78 -0.2 801,216 - fabG OKGIIK_03635 0.78 -1.9 801,216 - fabG OKGIIK_03635 0.78 -1.3 801,216 - fabG OKGIIK_03635 0.78 +0.2 801,216 - fabG OKGIIK_03635 0.78 +0.6 801,216 - fabG OKGIIK_03635 0.78 -0.8 801,216 - fabG OKGIIK_03635 0.78 -0.2 801,216 - fabG OKGIIK_03635 0.78 +0.1 801,216 - fabG OKGIIK_03635 0.78 -0.9 801,216 - fabG OKGIIK_03635 0.78 -0.4 801,216 - fabG OKGIIK_03635 0.78 -1.1 801,216 - fabG OKGIIK_03635 0.78 +0.8 801,216 - fabG OKGIIK_03635 0.78 -1.3 801,216 - fabG OKGIIK_03635 0.78 +0.4 801,216 - fabG OKGIIK_03635 0.78 +0.6 801,216 - fabG OKGIIK_03635 0.78 -1.1 801,216 - fabG OKGIIK_03635 0.78 -0.9 801,276 + fabG OKGIIK_03635 0.86 -1.9 801,276 + fabG OKGIIK_03635 0.86 -0.7 801,276 + fabG OKGIIK_03635 0.86 -0.0 801,276 + fabG OKGIIK_03635 0.86 -0.1 801,276 + fabG OKGIIK_03635 0.86 -1.5 801,277 - fabG OKGIIK_03635 0.87 -0.7 801,277 - fabG OKGIIK_03635 0.87 +0.5 801,277 - fabG OKGIIK_03635 0.87 -1.7 801,277 - fabG OKGIIK_03635 0.87 -1.2 801,312 + +0.6 801,313 - -0.5 801,364 + +1.3 801,364 + -2.6 801,364 + +0.1 801,365 - -0.5 801,365 - +0.4 801,365 - +0.9 801,480 - +1.5 801,480 - -0.7 801,480 - -0.5 801,480 - -0.3 801,480 - -1.7 801,480 - +0.6 801,480 - -0.5 801,480 - -0.2 801,481 + +0.7 801,482 - -0.3 801,482 - -0.1 801,523 - +0.1 801,523 - +2.3 801,523 - -2.1 801,523 - -0.1 801,523 - +1.1 801,717 + gluQRS OKGIIK_03640 0.26 +0.1 801,846 + gluQRS OKGIIK_03640 0.41 -1.1 801,846 + gluQRS OKGIIK_03640 0.41 +0.2 801,846 + gluQRS OKGIIK_03640 0.41 -0.5 801,846 + gluQRS OKGIIK_03640 0.41 -0.3 801,846 + gluQRS OKGIIK_03640 0.41 -1.1 801,846 + gluQRS OKGIIK_03640 0.41 +0.5 801,846 + gluQRS OKGIIK_03640 0.41 +0.7 801,847 - gluQRS OKGIIK_03640 0.41 +0.7 801,847 - gluQRS OKGIIK_03640 0.41 +1.1 801,847 - gluQRS OKGIIK_03640 0.41 -0.7 801,847 - gluQRS OKGIIK_03640 0.41 +1.1 801,847 - gluQRS OKGIIK_03640 0.41 -1.3 801,847 - gluQRS OKGIIK_03640 0.41 -1.2 801,847 - gluQRS OKGIIK_03640 0.41 +1.3 801,847 - gluQRS OKGIIK_03640 0.41 -0.6 801,847 - gluQRS OKGIIK_03640 0.41 -1.6 801,852 + gluQRS OKGIIK_03640 0.42 -1.1 801,852 + gluQRS OKGIIK_03640 0.42 -1.5 801,852 + gluQRS OKGIIK_03640 0.42 -0.1 801,852 + gluQRS OKGIIK_03640 0.42 +0.5 801,852 + gluQRS OKGIIK_03640 0.42 -1.1 801,852 - gluQRS OKGIIK_03640 0.42 -0.4 801,853 - gluQRS OKGIIK_03640 0.42 -0.3 801,853 - gluQRS OKGIIK_03640 0.42 -0.5 801,853 - gluQRS OKGIIK_03640 0.42 +0.6 801,853 - gluQRS OKGIIK_03640 0.42 -0.5 801,853 - gluQRS OKGIIK_03640 0.42 +1.3 801,853 - gluQRS OKGIIK_03640 0.42 +1.1 801,853 - gluQRS OKGIIK_03640 0.42 -0.7 801,853 - gluQRS OKGIIK_03640 0.42 +0.8 801,853 - gluQRS OKGIIK_03640 0.42 -0.3 801,853 - gluQRS OKGIIK_03640 0.42 -0.5 802,365 + -0.2 802,365 + +1.2 802,365 + -0.5 802,365 + +0.1 802,365 + -0.9 802,365 + -0.3 802,365 + -1.1 802,365 + +0.5 802,365 + +0.2 802,365 + -0.7 802,366 - +0.1 802,366 - -0.1 802,366 - +1.4 802,366 - +0.1 802,366 - -1.4 802,366 - +0.1 802,613 + htpX OKGIIK_03645 0.15 +0.1 802,614 - htpX OKGIIK_03645 0.15 -0.2 802,614 - htpX OKGIIK_03645 0.15 +1.3 802,796 + htpX OKGIIK_03645 0.35 -1.3 802,796 + htpX OKGIIK_03645 0.35 +0.6 802,796 + htpX OKGIIK_03645 0.35 -1.3 802,797 - htpX OKGIIK_03645 0.35 -0.4 802,797 - htpX OKGIIK_03645 0.35 -0.7 803,078 + htpX OKGIIK_03645 0.67 -0.5 803,251 + htpX OKGIIK_03645 0.86 -0.1 803,251 + htpX OKGIIK_03645 0.86 +0.3 803,251 + htpX OKGIIK_03645 0.86 +0.5 803,251 + htpX OKGIIK_03645 0.86 -0.4 803,252 - htpX OKGIIK_03645 0.86 -0.1 803,252 - htpX OKGIIK_03645 0.86 -0.8 803,252 - htpX OKGIIK_03645 0.86 -0.1 803,252 - htpX OKGIIK_03645 0.86 -1.3
Or see this region's nucleotide sequence