Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhaK and OKGIIK_03620 are separated by 151 nucleotides OKGIIK_03620 and OKGIIK_03625 are separated by 57 nucleotides OKGIIK_03625 and tcdA are separated by 104 nucleotides
OKGIIK_03615: yhaK - Pirin, at 797,828 to 798,703
yhaK
OKGIIK_03620: OKGIIK_03620 - DUF962 domain-containing protein, at 798,855 to 799,157
_03620
OKGIIK_03625: OKGIIK_03625 - Cytochrome C, at 799,215 to 799,604
_03625
OKGIIK_03630: tcdA - tRNA A37 threonylcarbamoyladenosine dehydratase, at 799,709 to 800,539
tcdA
Position (kb) 799
800 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 798.238 kb on - strand, within yhaK at 798.317 kb on + strand, within yhaK at 798.318 kb on - strand, within yhaK at 798.318 kb on - strand, within yhaK at 798.318 kb on - strand, within yhaK at 798.795 kb on + strand at 798.795 kb on + strand at 798.865 kb on - strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.885 kb on + strand at 798.886 kb on - strand, within OKGIIK_03620 at 798.886 kb on - strand, within OKGIIK_03620 at 798.886 kb on - strand, within OKGIIK_03620 at 798.886 kb on - strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.894 kb on + strand, within OKGIIK_03620 at 798.895 kb on + strand, within OKGIIK_03620 at 798.895 kb on - strand, within OKGIIK_03620 at 798.895 kb on - strand, within OKGIIK_03620 at 798.895 kb on - strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.994 kb on + strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 798.995 kb on - strand, within OKGIIK_03620 at 799.095 kb on + strand, within OKGIIK_03620 at 799.095 kb on + strand, within OKGIIK_03620 at 799.095 kb on + strand, within OKGIIK_03620 at 799.095 kb on + strand, within OKGIIK_03620 at 799.095 kb on + strand, within OKGIIK_03620 at 799.095 kb on + strand, within OKGIIK_03620 at 799.096 kb on - strand, within OKGIIK_03620 at 799.096 kb on - strand, within OKGIIK_03620 at 799.096 kb on - strand, within OKGIIK_03620 at 799.096 kb on - strand, within OKGIIK_03620 at 799.096 kb on - strand, within OKGIIK_03620 at 799.096 kb on - strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.122 kb on + strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.123 kb on - strand, within OKGIIK_03620 at 799.222 kb on + strand at 799.222 kb on + strand at 799.222 kb on + strand at 799.222 kb on + strand at 799.223 kb on - strand at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.425 kb on + strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.426 kb on - strand, within OKGIIK_03625 at 799.484 kb on - strand, within OKGIIK_03625 at 799.484 kb on - strand, within OKGIIK_03625 at 799.596 kb on + strand at 799.596 kb on + strand at 799.596 kb on + strand at 799.597 kb on - strand at 799.608 kb on + strand at 799.608 kb on + strand at 799.609 kb on - strand at 799.609 kb on - strand at 799.609 kb on - strand at 799.609 kb on - strand at 799.675 kb on + strand at 799.676 kb on + strand at 799.676 kb on + strand at 799.677 kb on - strand at 799.677 kb on - strand at 799.729 kb on + strand at 799.729 kb on + strand at 799.729 kb on + strand at 799.729 kb on + strand at 799.730 kb on - strand at 799.730 kb on - strand at 799.730 kb on - strand at 799.730 kb on - strand at 799.730 kb on - strand at 799.802 kb on + strand, within tcdA at 799.802 kb on + strand, within tcdA at 799.803 kb on - strand, within tcdA at 799.803 kb on - strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.985 kb on + strand, within tcdA at 799.986 kb on - strand, within tcdA at 799.986 kb on - strand, within tcdA at 799.986 kb on - strand, within tcdA at 799.986 kb on - strand, within tcdA at 799.986 kb on - strand, within tcdA at 799.986 kb on - strand, within tcdA at 799.986 kb on - strand, within tcdA at 800.108 kb on + strand, within tcdA at 800.109 kb on - strand, within tcdA at 800.337 kb on - strand, within tcdA at 800.337 kb on - strand, within tcdA at 800.357 kb on + strand, within tcdA at 800.358 kb on - strand, within tcdA at 800.379 kb on - strand, within tcdA at 800.538 kb on - strand at 800.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 798,238 - yhaK OKGIIK_03615 0.47 -0.2 798,317 + yhaK OKGIIK_03615 0.56 +0.2 798,318 - yhaK OKGIIK_03615 0.56 +0.1 798,318 - yhaK OKGIIK_03615 0.56 +1.3 798,318 - yhaK OKGIIK_03615 0.56 -0.3 798,795 + -2.1 798,795 + +0.1 798,865 - -0.7 798,885 + +0.2 798,885 + +0.3 798,885 + -0.5 798,885 + +0.3 798,885 + -0.7 798,885 + -1.6 798,885 + +0.9 798,885 + -0.4 798,886 - OKGIIK_03620 0.10 -1.5 798,886 - OKGIIK_03620 0.10 -0.4 798,886 - OKGIIK_03620 0.10 -0.2 798,886 - OKGIIK_03620 0.10 -0.4 798,894 + OKGIIK_03620 0.13 -1.3 798,894 + OKGIIK_03620 0.13 -1.9 798,894 + OKGIIK_03620 0.13 +1.6 798,894 + OKGIIK_03620 0.13 -0.3 798,894 + OKGIIK_03620 0.13 -1.1 798,894 + OKGIIK_03620 0.13 -0.5 798,894 + OKGIIK_03620 0.13 -0.3 798,894 + OKGIIK_03620 0.13 +0.3 798,894 + OKGIIK_03620 0.13 +0.2 798,894 + OKGIIK_03620 0.13 +0.3 798,895 + OKGIIK_03620 0.13 -0.4 798,895 - OKGIIK_03620 0.13 +0.6 798,895 - OKGIIK_03620 0.13 +1.1 798,895 - OKGIIK_03620 0.13 +0.1 798,994 + OKGIIK_03620 0.46 -0.7 798,994 + OKGIIK_03620 0.46 +2.4 798,994 + OKGIIK_03620 0.46 +0.1 798,994 + OKGIIK_03620 0.46 -0.2 798,994 + OKGIIK_03620 0.46 +1.1 798,994 + OKGIIK_03620 0.46 +0.5 798,994 + OKGIIK_03620 0.46 -3.8 798,994 + OKGIIK_03620 0.46 +0.3 798,994 + OKGIIK_03620 0.46 -0.4 798,994 + OKGIIK_03620 0.46 -0.9 798,994 + OKGIIK_03620 0.46 +1.4 798,994 + OKGIIK_03620 0.46 +0.2 798,994 + OKGIIK_03620 0.46 -0.2 798,994 + OKGIIK_03620 0.46 +0.9 798,995 - OKGIIK_03620 0.46 +1.5 798,995 - OKGIIK_03620 0.46 -0.1 798,995 - OKGIIK_03620 0.46 -0.5 798,995 - OKGIIK_03620 0.46 +0.1 798,995 - OKGIIK_03620 0.46 +0.6 798,995 - OKGIIK_03620 0.46 -0.2 798,995 - OKGIIK_03620 0.46 +0.4 798,995 - OKGIIK_03620 0.46 -0.3 798,995 - OKGIIK_03620 0.46 +0.1 798,995 - OKGIIK_03620 0.46 -0.4 798,995 - OKGIIK_03620 0.46 +0.3 798,995 - OKGIIK_03620 0.46 +1.1 798,995 - OKGIIK_03620 0.46 -2.3 798,995 - OKGIIK_03620 0.46 -0.7 799,095 + OKGIIK_03620 0.79 +0.6 799,095 + OKGIIK_03620 0.79 -1.3 799,095 + OKGIIK_03620 0.79 -0.3 799,095 + OKGIIK_03620 0.79 -2.3 799,095 + OKGIIK_03620 0.79 +1.4 799,095 + OKGIIK_03620 0.79 -0.3 799,096 - OKGIIK_03620 0.80 +0.1 799,096 - OKGIIK_03620 0.80 -0.5 799,096 - OKGIIK_03620 0.80 -0.1 799,096 - OKGIIK_03620 0.80 -0.7 799,096 - OKGIIK_03620 0.80 -1.0 799,096 - OKGIIK_03620 0.80 -0.0 799,122 + OKGIIK_03620 0.88 +0.2 799,122 + OKGIIK_03620 0.88 -0.6 799,122 + OKGIIK_03620 0.88 +0.8 799,122 + OKGIIK_03620 0.88 -0.9 799,122 + OKGIIK_03620 0.88 -0.7 799,122 + OKGIIK_03620 0.88 +1.2 799,122 + OKGIIK_03620 0.88 -0.5 799,122 + OKGIIK_03620 0.88 -0.2 799,123 - OKGIIK_03620 0.88 -1.1 799,123 - OKGIIK_03620 0.88 -1.1 799,123 - OKGIIK_03620 0.88 -1.6 799,123 - OKGIIK_03620 0.88 -0.5 799,123 - OKGIIK_03620 0.88 +0.6 799,123 - OKGIIK_03620 0.88 -1.1 799,123 - OKGIIK_03620 0.88 -1.3 799,123 - OKGIIK_03620 0.88 -0.5 799,123 - OKGIIK_03620 0.88 -2.7 799,123 - OKGIIK_03620 0.88 +0.7 799,123 - OKGIIK_03620 0.88 -1.7 799,123 - OKGIIK_03620 0.88 -0.4 799,222 + -1.1 799,222 + -1.1 799,222 + -0.2 799,222 + +0.5 799,223 - -1.7 799,425 + OKGIIK_03625 0.54 +0.1 799,425 + OKGIIK_03625 0.54 -0.5 799,425 + OKGIIK_03625 0.54 -2.7 799,425 + OKGIIK_03625 0.54 -0.7 799,425 + OKGIIK_03625 0.54 +0.1 799,425 + OKGIIK_03625 0.54 -0.8 799,425 + OKGIIK_03625 0.54 -0.1 799,425 + OKGIIK_03625 0.54 -0.9 799,425 + OKGIIK_03625 0.54 +0.8 799,425 + OKGIIK_03625 0.54 -1.3 799,425 + OKGIIK_03625 0.54 -1.6 799,425 + OKGIIK_03625 0.54 -1.3 799,425 + OKGIIK_03625 0.54 -0.7 799,425 + OKGIIK_03625 0.54 -0.2 799,425 + OKGIIK_03625 0.54 +0.1 799,425 + OKGIIK_03625 0.54 -0.6 799,426 - OKGIIK_03625 0.54 -0.2 799,426 - OKGIIK_03625 0.54 +1.0 799,426 - OKGIIK_03625 0.54 -2.8 799,426 - OKGIIK_03625 0.54 -0.8 799,426 - OKGIIK_03625 0.54 -0.8 799,426 - OKGIIK_03625 0.54 -0.9 799,426 - OKGIIK_03625 0.54 -0.9 799,426 - OKGIIK_03625 0.54 -0.5 799,484 - OKGIIK_03625 0.69 -0.1 799,484 - OKGIIK_03625 0.69 -1.0 799,596 + +0.6 799,596 + -2.3 799,596 + -2.6 799,597 - +0.1 799,608 + +0.3 799,608 + -1.6 799,609 - -2.3 799,609 - -1.3 799,609 - -1.3 799,609 - +0.3 799,675 + +0.3 799,676 + +0.2 799,676 + -0.2 799,677 - +1.1 799,677 - -1.9 799,729 + +0.6 799,729 + -0.5 799,729 + +0.2 799,729 + -0.8 799,730 - -1.0 799,730 - +0.5 799,730 - +0.4 799,730 - -1.1 799,730 - -0.7 799,802 + tcdA OKGIIK_03630 0.11 +0.1 799,802 + tcdA OKGIIK_03630 0.11 -0.9 799,803 - tcdA OKGIIK_03630 0.11 -0.3 799,803 - tcdA OKGIIK_03630 0.11 +0.5 799,985 + tcdA OKGIIK_03630 0.33 +0.5 799,985 + tcdA OKGIIK_03630 0.33 +0.4 799,985 + tcdA OKGIIK_03630 0.33 +1.6 799,985 + tcdA OKGIIK_03630 0.33 +0.2 799,985 + tcdA OKGIIK_03630 0.33 -0.7 799,985 + tcdA OKGIIK_03630 0.33 -1.2 799,985 + tcdA OKGIIK_03630 0.33 -2.1 799,986 - tcdA OKGIIK_03630 0.33 +0.9 799,986 - tcdA OKGIIK_03630 0.33 -1.3 799,986 - tcdA OKGIIK_03630 0.33 -0.5 799,986 - tcdA OKGIIK_03630 0.33 +0.2 799,986 - tcdA OKGIIK_03630 0.33 -0.3 799,986 - tcdA OKGIIK_03630 0.33 +0.2 799,986 - tcdA OKGIIK_03630 0.33 -0.4 800,108 + tcdA OKGIIK_03630 0.48 +0.6 800,109 - tcdA OKGIIK_03630 0.48 +1.1 800,337 - tcdA OKGIIK_03630 0.76 +0.4 800,337 - tcdA OKGIIK_03630 0.76 -1.1 800,357 + tcdA OKGIIK_03630 0.78 +0.8 800,358 - tcdA OKGIIK_03630 0.78 -0.7 800,379 - tcdA OKGIIK_03630 0.81 -1.8 800,538 - -0.2 800,538 - +0.5
Or see this region's nucleotide sequence