Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliG and OKGIIK_02755 overlap by 1 nucleotides OKGIIK_02755 and fliI overlap by 4 nucleotides fliI and fliJ overlap by 4 nucleotides fliJ and OKGIIK_02770 are separated by 64 nucleotides
OKGIIK_02750: fliG - flagellar motor switch protein FliG, at 636,862 to 637,878
fliG
OKGIIK_02755: OKGIIK_02755 - Flagellar assembly protein FliH, at 637,878 to 638,507
_02755
OKGIIK_02760: fliI - flagellar protein export ATPase FliI, at 638,504 to 639,883
fliI
OKGIIK_02765: fliJ - flagellar export protein FliJ, at 639,880 to 640,305
fliJ
OKGIIK_02770: OKGIIK_02770 - Flg-hook domain-containing protein, at 640,370 to 641,452
_02770
Position (kb) 638
639
640 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.870 kb on + strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 637.871 kb on - strand at 638.034 kb on + strand, within OKGIIK_02755 at 638.034 kb on + strand, within OKGIIK_02755 at 638.034 kb on + strand, within OKGIIK_02755 at 638.034 kb on + strand, within OKGIIK_02755 at 638.034 kb on + strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.035 kb on - strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.112 kb on + strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.113 kb on - strand, within OKGIIK_02755 at 638.881 kb on - strand, within fliI at 639.095 kb on + strand, within fliI at 639.096 kb on - strand, within fliI at 639.096 kb on - strand, within fliI at 639.257 kb on + strand, within fliI at 639.257 kb on + strand, within fliI at 639.258 kb on - strand, within fliI at 639.258 kb on - strand, within fliI at 639.258 kb on - strand, within fliI at 639.258 kb on - strand, within fliI at 639.333 kb on - strand, within fliI at 639.333 kb on - strand, within fliI at 639.479 kb on + strand, within fliI at 639.480 kb on - strand, within fliI at 639.480 kb on - strand, within fliI at 639.480 kb on - strand, within fliI at 639.480 kb on - strand, within fliI at 639.507 kb on - strand, within fliI at 639.507 kb on - strand, within fliI at 639.556 kb on + strand, within fliI at 639.557 kb on - strand, within fliI at 639.557 kb on - strand, within fliI at 639.557 kb on - strand, within fliI at 639.689 kb on + strand, within fliI at 639.689 kb on + strand, within fliI at 639.689 kb on + strand, within fliI at 639.689 kb on + strand, within fliI at 639.689 kb on + strand, within fliI at 639.689 kb on + strand, within fliI at 639.690 kb on - strand, within fliI at 639.690 kb on - strand, within fliI at 639.690 kb on - strand, within fliI at 639.690 kb on - strand, within fliI at 639.690 kb on - strand, within fliI at 639.690 kb on - strand, within fliI at 639.734 kb on + strand, within fliI at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.027 kb on + strand, within fliJ at 640.028 kb on - strand, within fliJ at 640.028 kb on - strand, within fliJ at 640.028 kb on - strand, within fliJ at 640.028 kb on - strand, within fliJ at 640.028 kb on - strand, within fliJ at 640.028 kb on - strand, within fliJ at 640.518 kb on + strand, within OKGIIK_02770 at 640.518 kb on + strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770 at 640.519 kb on - strand, within OKGIIK_02770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 637,870 + +0.3 637,870 + -0.3 637,870 + +0.1 637,870 + +1.0 637,870 + -0.8 637,870 + -1.2 637,870 + +1.0 637,870 + +1.2 637,870 + -1.3 637,870 + -0.2 637,870 + -1.6 637,870 + +0.5 637,870 + -0.7 637,870 + -1.3 637,870 + +1.6 637,870 + +1.2 637,870 + +0.3 637,870 + +0.8 637,870 + -0.0 637,870 + -1.1 637,870 + -1.2 637,870 + +0.1 637,871 - +0.4 637,871 - +2.0 637,871 - +0.2 637,871 - +0.7 637,871 - +0.4 637,871 - +1.3 637,871 - +0.2 637,871 - -1.8 637,871 - -1.0 637,871 - -0.0 637,871 - -1.6 637,871 - +0.4 637,871 - -0.2 637,871 - +0.8 637,871 - -1.3 638,034 + OKGIIK_02755 0.25 +0.7 638,034 + OKGIIK_02755 0.25 -1.6 638,034 + OKGIIK_02755 0.25 -0.6 638,034 + OKGIIK_02755 0.25 -0.0 638,034 + OKGIIK_02755 0.25 -1.0 638,035 - OKGIIK_02755 0.25 -0.8 638,035 - OKGIIK_02755 0.25 +0.3 638,035 - OKGIIK_02755 0.25 -1.3 638,035 - OKGIIK_02755 0.25 +2.0 638,035 - OKGIIK_02755 0.25 -0.6 638,035 - OKGIIK_02755 0.25 -0.0 638,035 - OKGIIK_02755 0.25 -0.6 638,112 + OKGIIK_02755 0.37 -0.7 638,112 + OKGIIK_02755 0.37 -0.1 638,112 + OKGIIK_02755 0.37 +0.4 638,112 + OKGIIK_02755 0.37 -0.0 638,112 + OKGIIK_02755 0.37 -0.2 638,112 + OKGIIK_02755 0.37 -0.5 638,112 + OKGIIK_02755 0.37 -0.4 638,112 + OKGIIK_02755 0.37 -0.0 638,112 + OKGIIK_02755 0.37 +1.2 638,112 + OKGIIK_02755 0.37 +0.6 638,113 - OKGIIK_02755 0.37 +0.7 638,113 - OKGIIK_02755 0.37 +0.3 638,113 - OKGIIK_02755 0.37 +0.7 638,113 - OKGIIK_02755 0.37 +0.5 638,113 - OKGIIK_02755 0.37 +0.8 638,113 - OKGIIK_02755 0.37 +0.3 638,113 - OKGIIK_02755 0.37 +0.1 638,113 - OKGIIK_02755 0.37 +0.6 638,113 - OKGIIK_02755 0.37 +0.1 638,881 - fliI OKGIIK_02760 0.27 +0.3 639,095 + fliI OKGIIK_02760 0.43 +0.8 639,096 - fliI OKGIIK_02760 0.43 -0.0 639,096 - fliI OKGIIK_02760 0.43 -0.1 639,257 + fliI OKGIIK_02760 0.55 +0.2 639,257 + fliI OKGIIK_02760 0.55 +2.0 639,258 - fliI OKGIIK_02760 0.55 +0.7 639,258 - fliI OKGIIK_02760 0.55 -0.6 639,258 - fliI OKGIIK_02760 0.55 +0.8 639,258 - fliI OKGIIK_02760 0.55 +0.5 639,333 - fliI OKGIIK_02760 0.60 -0.1 639,333 - fliI OKGIIK_02760 0.60 +1.0 639,479 + fliI OKGIIK_02760 0.71 -0.0 639,480 - fliI OKGIIK_02760 0.71 -0.8 639,480 - fliI OKGIIK_02760 0.71 +0.5 639,480 - fliI OKGIIK_02760 0.71 +1.0 639,480 - fliI OKGIIK_02760 0.71 +0.3 639,507 - fliI OKGIIK_02760 0.73 -0.0 639,507 - fliI OKGIIK_02760 0.73 -1.0 639,556 + fliI OKGIIK_02760 0.76 +1.0 639,557 - fliI OKGIIK_02760 0.76 -0.1 639,557 - fliI OKGIIK_02760 0.76 +0.4 639,557 - fliI OKGIIK_02760 0.76 +0.4 639,689 + fliI OKGIIK_02760 0.86 +4.8 639,689 + fliI OKGIIK_02760 0.86 +1.0 639,689 + fliI OKGIIK_02760 0.86 -0.7 639,689 + fliI OKGIIK_02760 0.86 +2.6 639,689 + fliI OKGIIK_02760 0.86 -0.7 639,689 + fliI OKGIIK_02760 0.86 +0.7 639,690 - fliI OKGIIK_02760 0.86 +0.6 639,690 - fliI OKGIIK_02760 0.86 +0.0 639,690 - fliI OKGIIK_02760 0.86 +0.1 639,690 - fliI OKGIIK_02760 0.86 +0.0 639,690 - fliI OKGIIK_02760 0.86 +1.6 639,690 - fliI OKGIIK_02760 0.86 +0.9 639,734 + fliI OKGIIK_02760 0.89 -0.4 640,027 + fliJ OKGIIK_02765 0.35 +0.3 640,027 + fliJ OKGIIK_02765 0.35 -1.6 640,027 + fliJ OKGIIK_02765 0.35 -0.3 640,027 + fliJ OKGIIK_02765 0.35 +0.0 640,027 + fliJ OKGIIK_02765 0.35 +2.0 640,027 + fliJ OKGIIK_02765 0.35 -1.1 640,027 + fliJ OKGIIK_02765 0.35 +0.6 640,027 + fliJ OKGIIK_02765 0.35 -0.6 640,028 - fliJ OKGIIK_02765 0.35 +2.0 640,028 - fliJ OKGIIK_02765 0.35 -1.3 640,028 - fliJ OKGIIK_02765 0.35 +0.4 640,028 - fliJ OKGIIK_02765 0.35 +1.0 640,028 - fliJ OKGIIK_02765 0.35 +0.7 640,028 - fliJ OKGIIK_02765 0.35 -1.6 640,518 + OKGIIK_02770 0.14 +0.7 640,518 + OKGIIK_02770 0.14 +0.0 640,519 - OKGIIK_02770 0.14 +0.7 640,519 - OKGIIK_02770 0.14 +0.4 640,519 - OKGIIK_02770 0.14 +0.0 640,519 - OKGIIK_02770 0.14 +2.0 640,519 - OKGIIK_02770 0.14 -0.1 640,519 - OKGIIK_02770 0.14 +0.5 640,519 - OKGIIK_02770 0.14 +0.8 640,519 - OKGIIK_02770 0.14 +0.2 640,519 - OKGIIK_02770 0.14 -0.3 640,519 - OKGIIK_02770 0.14 -0.7 640,519 - OKGIIK_02770 0.14 +1.3 640,519 - OKGIIK_02770 0.14 +2.0 640,519 - OKGIIK_02770 0.14 +1.7
Or see this region's nucleotide sequence