Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_02725 and OKGIIK_02730 are separated by 84 nucleotides OKGIIK_02730 and OKGIIK_02735 are separated by 345 nucleotides OKGIIK_02735 and fliE are separated by 126 nucleotides fliE and fliF are separated by 47 nucleotides
OKGIIK_02725: OKGIIK_02725 - hypothetical protein, at 631,996 to 632,274
_02725
OKGIIK_02730: OKGIIK_02730 - PilZ-2 domain-containing protein, at 632,359 to 632,913
_02730
OKGIIK_02735: OKGIIK_02735 - Fis family transcriptional regulator, at 633,259 to 634,683
_02735
OKGIIK_02740: fliE - flagellar hook-basal body complex protein FliE, at 634,810 to 635,139
fliE
OKGIIK_02745: fliF - flagellar M-ring protein FliF, at 635,187 to 636,872
fliF
Position (kb) 633
634
635 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 632.260 kb on + strand at 632.260 kb on + strand at 632.260 kb on + strand at 632.260 kb on + strand at 632.261 kb on - strand at 632.495 kb on + strand, within OKGIIK_02730 at 632.605 kb on - strand, within OKGIIK_02730 at 632.861 kb on + strand at 632.861 kb on + strand at 632.861 kb on + strand at 632.861 kb on + strand at 632.861 kb on + strand at 632.861 kb on + strand at 632.861 kb on + strand at 632.862 kb on - strand at 632.862 kb on - strand at 632.862 kb on - strand at 632.862 kb on - strand at 632.862 kb on - strand at 632.862 kb on - strand at 632.957 kb on + strand at 632.957 kb on + strand at 632.957 kb on + strand at 632.957 kb on + strand at 632.957 kb on - strand at 632.958 kb on - strand at 632.958 kb on - strand at 632.958 kb on - strand at 633.019 kb on + strand at 633.020 kb on - strand at 633.034 kb on - strand at 633.034 kb on - strand at 633.034 kb on - strand at 633.037 kb on + strand at 633.037 kb on + strand at 633.057 kb on + strand at 633.057 kb on + strand at 633.057 kb on + strand at 633.058 kb on - strand at 633.058 kb on - strand at 633.058 kb on - strand at 633.058 kb on - strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.074 kb on + strand at 633.075 kb on - strand at 633.075 kb on - strand at 633.075 kb on - strand at 633.075 kb on - strand at 633.075 kb on - strand at 633.075 kb on - strand at 633.154 kb on + strand at 633.154 kb on + strand at 633.154 kb on + strand at 633.154 kb on + strand at 633.155 kb on - strand at 633.155 kb on - strand at 633.155 kb on - strand at 633.155 kb on - strand at 633.155 kb on - strand at 633.155 kb on - strand at 633.306 kb on - strand at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.406 kb on + strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.407 kb on - strand, within OKGIIK_02735 at 633.613 kb on + strand, within OKGIIK_02735 at 633.613 kb on + strand, within OKGIIK_02735 at 633.613 kb on + strand, within OKGIIK_02735 at 633.613 kb on + strand, within OKGIIK_02735 at 633.614 kb on - strand, within OKGIIK_02735 at 633.614 kb on - strand, within OKGIIK_02735 at 633.614 kb on - strand, within OKGIIK_02735 at 633.614 kb on - strand, within OKGIIK_02735 at 633.664 kb on + strand, within OKGIIK_02735 at 634.015 kb on + strand, within OKGIIK_02735 at 634.015 kb on + strand, within OKGIIK_02735 at 634.015 kb on + strand, within OKGIIK_02735 at 634.016 kb on - strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.129 kb on + strand, within OKGIIK_02735 at 634.130 kb on - strand, within OKGIIK_02735 at 634.130 kb on - strand, within OKGIIK_02735 at 634.130 kb on - strand, within OKGIIK_02735 at 634.130 kb on - strand, within OKGIIK_02735 at 634.130 kb on - strand, within OKGIIK_02735 at 634.130 kb on - strand, within OKGIIK_02735 at 634.384 kb on + strand, within OKGIIK_02735 at 634.384 kb on + strand, within OKGIIK_02735 at 634.384 kb on + strand, within OKGIIK_02735 at 634.385 kb on - strand, within OKGIIK_02735 at 634.385 kb on - strand, within OKGIIK_02735 at 634.385 kb on - strand, within OKGIIK_02735 at 634.385 kb on - strand, within OKGIIK_02735 at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.654 kb on + strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.655 kb on - strand at 634.681 kb on + strand at 634.681 kb on + strand at 634.681 kb on + strand at 634.682 kb on - strand at 634.682 kb on - strand at 634.682 kb on - strand at 634.682 kb on - strand at 634.756 kb on + strand at 634.756 kb on + strand at 634.756 kb on + strand at 634.756 kb on + strand at 634.756 kb on + strand at 634.756 kb on + strand at 634.756 kb on + strand at 634.757 kb on - strand at 634.757 kb on - strand at 634.757 kb on - strand at 634.757 kb on - strand at 634.757 kb on - strand at 634.757 kb on - strand at 634.757 kb on - strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.110 kb on + strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.111 kb on - strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.121 kb on + strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.122 kb on - strand at 635.225 kb on + strand at 635.226 kb on - strand at 635.226 kb on - strand at 635.352 kb on + strand at 635.352 kb on + strand at 635.352 kb on + strand at 635.352 kb on + strand at 635.353 kb on - strand at 635.353 kb on - strand at 635.353 kb on - strand at 635.353 kb on - strand at 635.353 kb on - strand at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.364 kb on + strand, within fliF at 635.365 kb on - strand, within fliF at 635.365 kb on - strand, within fliF at 635.365 kb on - strand, within fliF at 635.365 kb on - strand, within fliF at 635.365 kb on - strand, within fliF at 635.365 kb on - strand, within fliF at 635.365 kb on - strand, within fliF at 635.430 kb on + strand, within fliF at 635.431 kb on - strand, within fliF at 635.431 kb on - strand, within fliF at 635.431 kb on - strand, within fliF at 635.431 kb on - strand, within fliF
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 632,260 + +0.7 632,260 + +0.4 632,260 + -0.0 632,260 + +0.4 632,261 - +1.3 632,495 + OKGIIK_02730 0.25 -1.0 632,605 - OKGIIK_02730 0.44 +0.3 632,861 + -0.2 632,861 + +0.4 632,861 + -1.5 632,861 + +0.7 632,861 + -0.0 632,861 + +1.8 632,861 + -1.3 632,862 - -0.0 632,862 - -0.4 632,862 - +4.2 632,862 - +0.1 632,862 - -1.6 632,862 - -0.5 632,957 + -1.6 632,957 + -0.0 632,957 + -0.3 632,957 + -0.1 632,957 - +0.1 632,958 - -1.3 632,958 - +0.3 632,958 - -0.0 633,019 + +0.5 633,020 - +0.8 633,034 - -0.7 633,034 - +0.7 633,034 - +1.3 633,037 + -0.0 633,037 + +2.0 633,057 + +0.4 633,057 + +0.4 633,057 + +2.3 633,058 - +0.3 633,058 - +2.8 633,058 - -1.3 633,058 - -1.3 633,074 + +0.2 633,074 + +0.4 633,074 + -0.0 633,074 + -0.6 633,074 + -1.3 633,074 + -2.0 633,074 + +0.7 633,074 + +0.3 633,074 + +0.1 633,074 + +2.6 633,075 - -0.0 633,075 - -0.2 633,075 - -0.5 633,075 - -0.0 633,075 - -1.3 633,075 - +0.1 633,154 + -0.2 633,154 + -2.0 633,154 + +1.0 633,154 + -0.5 633,155 - +1.6 633,155 - -0.0 633,155 - +0.7 633,155 - -2.2 633,155 - +1.1 633,155 - +1.3 633,306 - -0.2 633,406 + OKGIIK_02735 0.10 +1.0 633,406 + OKGIIK_02735 0.10 -0.6 633,406 + OKGIIK_02735 0.10 +0.7 633,406 + OKGIIK_02735 0.10 +0.7 633,406 + OKGIIK_02735 0.10 +1.1 633,406 + OKGIIK_02735 0.10 -0.9 633,406 + OKGIIK_02735 0.10 +2.1 633,406 + OKGIIK_02735 0.10 +2.0 633,406 + OKGIIK_02735 0.10 -0.2 633,406 + OKGIIK_02735 0.10 -0.6 633,406 + OKGIIK_02735 0.10 -0.2 633,406 + OKGIIK_02735 0.10 -0.0 633,406 + OKGIIK_02735 0.10 -0.2 633,406 + OKGIIK_02735 0.10 +0.7 633,406 + OKGIIK_02735 0.10 -0.0 633,406 + OKGIIK_02735 0.10 -0.8 633,406 + OKGIIK_02735 0.10 +1.0 633,406 + OKGIIK_02735 0.10 -2.2 633,406 + OKGIIK_02735 0.10 +1.6 633,407 - OKGIIK_02735 0.10 -2.6 633,407 - OKGIIK_02735 0.10 +0.4 633,407 - OKGIIK_02735 0.10 -0.3 633,407 - OKGIIK_02735 0.10 +1.0 633,407 - OKGIIK_02735 0.10 +0.4 633,407 - OKGIIK_02735 0.10 -0.7 633,407 - OKGIIK_02735 0.10 -1.6 633,407 - OKGIIK_02735 0.10 +1.8 633,407 - OKGIIK_02735 0.10 +3.6 633,407 - OKGIIK_02735 0.10 +0.7 633,407 - OKGIIK_02735 0.10 +0.7 633,407 - OKGIIK_02735 0.10 +1.0 633,407 - OKGIIK_02735 0.10 +3.0 633,407 - OKGIIK_02735 0.10 -0.0 633,613 + OKGIIK_02735 0.25 +0.7 633,613 + OKGIIK_02735 0.25 -1.5 633,613 + OKGIIK_02735 0.25 +1.5 633,613 + OKGIIK_02735 0.25 -1.6 633,614 - OKGIIK_02735 0.25 -0.2 633,614 - OKGIIK_02735 0.25 +0.3 633,614 - OKGIIK_02735 0.25 -1.6 633,614 - OKGIIK_02735 0.25 +1.0 633,664 + OKGIIK_02735 0.28 -0.3 634,015 + OKGIIK_02735 0.53 -1.0 634,015 + OKGIIK_02735 0.53 -0.7 634,015 + OKGIIK_02735 0.53 -0.0 634,016 - OKGIIK_02735 0.53 +1.5 634,129 + OKGIIK_02735 0.61 +0.3 634,129 + OKGIIK_02735 0.61 -2.0 634,129 + OKGIIK_02735 0.61 +2.0 634,129 + OKGIIK_02735 0.61 -0.7 634,129 + OKGIIK_02735 0.61 +0.8 634,129 + OKGIIK_02735 0.61 +0.9 634,129 + OKGIIK_02735 0.61 -0.1 634,130 - OKGIIK_02735 0.61 -1.5 634,130 - OKGIIK_02735 0.61 +0.9 634,130 - OKGIIK_02735 0.61 -0.2 634,130 - OKGIIK_02735 0.61 -1.0 634,130 - OKGIIK_02735 0.61 -0.4 634,130 - OKGIIK_02735 0.61 +1.0 634,384 + OKGIIK_02735 0.79 -0.8 634,384 + OKGIIK_02735 0.79 +1.0 634,384 + OKGIIK_02735 0.79 -2.4 634,385 - OKGIIK_02735 0.79 -1.2 634,385 - OKGIIK_02735 0.79 -0.4 634,385 - OKGIIK_02735 0.79 -2.0 634,385 - OKGIIK_02735 0.79 +2.2 634,654 + -2.5 634,654 + -1.4 634,654 + +2.0 634,654 + +2.0 634,654 + +1.0 634,654 + -1.8 634,654 + -1.3 634,654 + -0.8 634,654 + +0.3 634,655 - +0.2 634,655 - +1.7 634,655 - +0.7 634,655 - +1.0 634,655 - +1.7 634,655 - -0.0 634,655 - -0.0 634,655 - -1.7 634,655 - -2.1 634,681 + -2.3 634,681 + -2.0 634,681 + -1.8 634,682 - +0.7 634,682 - -0.9 634,682 - -0.6 634,682 - -1.0 634,756 + -0.6 634,756 + +2.2 634,756 + +1.0 634,756 + -1.6 634,756 + -1.7 634,756 + +1.0 634,756 + -0.1 634,757 - -0.3 634,757 - +0.5 634,757 - +1.0 634,757 - +0.4 634,757 - +1.4 634,757 - -2.8 634,757 - -0.3 635,110 + -0.2 635,110 + +1.4 635,110 + -1.3 635,110 + +0.6 635,110 + -0.3 635,110 + -0.0 635,110 + -0.0 635,110 + +0.2 635,111 - +1.0 635,111 - -1.1 635,111 - +1.4 635,111 - -0.1 635,111 - +0.3 635,111 - +0.4 635,111 - -2.0 635,111 - -0.3 635,111 - -2.0 635,111 - +0.2 635,111 - +1.0 635,111 - +0.2 635,111 - +0.7 635,111 - -0.6 635,121 + +2.3 635,121 + +1.1 635,121 + -1.6 635,121 + -1.0 635,121 + -0.4 635,121 + -0.3 635,121 + +1.0 635,121 + +0.3 635,121 + +1.3 635,122 - +0.2 635,122 - -0.1 635,122 - -1.6 635,122 - -1.3 635,122 - -0.9 635,122 - -1.2 635,122 - -1.5 635,122 - +0.1 635,122 - -0.6 635,122 - +1.5 635,122 - -1.0 635,122 - +0.1 635,225 + -0.9 635,226 - +2.6 635,226 - -0.8 635,352 + +1.6 635,352 + -0.4 635,352 + +0.1 635,352 + -1.0 635,353 - -0.6 635,353 - -0.6 635,353 - +1.0 635,353 - +0.5 635,353 - -0.9 635,364 + fliF OKGIIK_02745 0.10 -0.4 635,364 + fliF OKGIIK_02745 0.10 +0.7 635,364 + fliF OKGIIK_02745 0.10 +1.0 635,364 + fliF OKGIIK_02745 0.10 +1.0 635,364 + fliF OKGIIK_02745 0.10 +1.0 635,364 + fliF OKGIIK_02745 0.10 +1.6 635,364 + fliF OKGIIK_02745 0.10 -0.6 635,364 + fliF OKGIIK_02745 0.10 -1.7 635,364 + fliF OKGIIK_02745 0.10 -1.9 635,365 - fliF OKGIIK_02745 0.11 +0.3 635,365 - fliF OKGIIK_02745 0.11 -1.3 635,365 - fliF OKGIIK_02745 0.11 -2.0 635,365 - fliF OKGIIK_02745 0.11 +0.6 635,365 - fliF OKGIIK_02745 0.11 +1.0 635,365 - fliF OKGIIK_02745 0.11 +0.5 635,365 - fliF OKGIIK_02745 0.11 -0.0 635,430 + fliF OKGIIK_02745 0.14 +0.3 635,431 - fliF OKGIIK_02745 0.14 -0.5 635,431 - fliF OKGIIK_02745 0.14 -0.3 635,431 - fliF OKGIIK_02745 0.14 -0.8 635,431 - fliF OKGIIK_02745 0.14 -0.1
Or see this region's nucleotide sequence