Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgI and flgJ are separated by 14 nucleotides flgJ and OKGIIK_02695 overlap by 4 nucleotides OKGIIK_02695 and flgK overlap by 8 nucleotides
OKGIIK_02685: flgI - Flagellar basal body P-ring protein FlgI, at 622,563 to 623,732
flgI
OKGIIK_02690: flgJ - flagellar assembly peptidoglycan hydrolase FlgJ, at 623,747 to 624,814
flgJ
OKGIIK_02695: OKGIIK_02695 - hypothetical protein, at 624,811 to 625,461
_02695
OKGIIK_02700: flgK - flagellar hook-associated protein FlgK, at 625,454 to 627,328
flgK
Position (kb) 623
624
625 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 623.260 kb on - strand, within flgI at 623.730 kb on + strand at 623.730 kb on + strand at 623.731 kb on - strand at 623.731 kb on - strand at 624.539 kb on + strand, within flgJ at 624.539 kb on + strand, within flgJ at 624.539 kb on + strand, within flgJ at 624.540 kb on - strand, within flgJ at 624.540 kb on - strand, within flgJ at 624.584 kb on + strand, within flgJ at 624.584 kb on + strand, within flgJ at 624.585 kb on - strand, within flgJ at 624.614 kb on + strand, within flgJ at 624.615 kb on - strand, within flgJ at 624.644 kb on + strand, within flgJ at 624.644 kb on + strand, within flgJ at 624.710 kb on + strand at 624.710 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.728 kb on + strand at 624.729 kb on - strand at 624.729 kb on - strand at 624.729 kb on - strand at 624.729 kb on - strand at 625.467 kb on + strand at 625.467 kb on + strand at 625.469 kb on - strand at 625.480 kb on - strand at 625.543 kb on + strand at 625.543 kb on + strand at 625.544 kb on - strand at 625.544 kb on - strand at 625.544 kb on - strand at 625.553 kb on + strand at 625.553 kb on + strand at 625.554 kb on - strand at 625.554 kb on - strand at 625.574 kb on + strand at 625.575 kb on - strand at 625.575 kb on - strand at 625.575 kb on - strand at 625.575 kb on - strand at 625.575 kb on - strand at 625.608 kb on - strand at 625.608 kb on - strand at 625.608 kb on - strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.610 kb on + strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.611 kb on - strand at 625.652 kb on + strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK at 625.653 kb on - strand, within flgK
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 623,260 - flgI OKGIIK_02685 0.60 -1.8 623,730 + -2.7 623,730 + -2.4 623,731 - -1.5 623,731 - +0.3 624,539 + flgJ OKGIIK_02690 0.74 +0.5 624,539 + flgJ OKGIIK_02690 0.74 +0.4 624,539 + flgJ OKGIIK_02690 0.74 +0.9 624,540 - flgJ OKGIIK_02690 0.74 +1.4 624,540 - flgJ OKGIIK_02690 0.74 +2.6 624,584 + flgJ OKGIIK_02690 0.78 -0.9 624,584 + flgJ OKGIIK_02690 0.78 -0.0 624,585 - flgJ OKGIIK_02690 0.78 +1.0 624,614 + flgJ OKGIIK_02690 0.81 -1.1 624,615 - flgJ OKGIIK_02690 0.81 +0.5 624,644 + flgJ OKGIIK_02690 0.84 -1.3 624,644 + flgJ OKGIIK_02690 0.84 -0.0 624,710 + +1.0 624,710 + +0.3 624,728 + -1.0 624,728 + +1.0 624,728 + +0.6 624,728 + -0.3 624,728 + -0.4 624,728 + -1.0 624,728 + +0.3 624,728 + +0.3 624,728 + +0.3 624,728 + -0.0 624,729 - +0.1 624,729 - -0.2 624,729 - -0.5 624,729 - +1.4 625,467 + -1.8 625,467 + -2.0 625,469 - -2.9 625,480 - -0.3 625,543 + +1.4 625,543 + -1.0 625,544 - +1.0 625,544 - -1.9 625,544 - +1.6 625,553 + +1.6 625,553 + +0.3 625,554 - +0.7 625,554 - -1.7 625,574 + -1.9 625,575 - +1.0 625,575 - -0.7 625,575 - -0.3 625,575 - -0.7 625,575 - -0.6 625,608 - -0.0 625,608 - +1.1 625,608 - -1.0 625,610 + -0.6 625,610 + -0.0 625,610 + -0.9 625,610 + -1.0 625,610 + -0.7 625,610 + +0.3 625,610 + +2.0 625,610 + +0.5 625,610 + +3.3 625,611 - -0.5 625,611 - +0.1 625,611 - +0.3 625,611 - -0.0 625,611 - -0.1 625,611 - -0.3 625,611 - -1.3 625,611 - +0.6 625,611 - -2.5 625,611 - +0.7 625,611 - +1.0 625,611 - -0.0 625,611 - -0.2 625,652 + flgK OKGIIK_02700 0.11 +0.5 625,653 - flgK OKGIIK_02700 0.11 -0.1 625,653 - flgK OKGIIK_02700 0.11 +0.2 625,653 - flgK OKGIIK_02700 0.11 -1.6 625,653 - flgK OKGIIK_02700 0.11 +0.2 625,653 - flgK OKGIIK_02700 0.11 -1.8 625,653 - flgK OKGIIK_02700 0.11 -1.0
Or see this region's nucleotide sequence