Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00395 and OKGIIK_00400 are separated by 270 nucleotides OKGIIK_00400 and tsaA overlap by 6 nucleotides tsaA and OKGIIK_00410 are separated by 58 nucleotides OKGIIK_00410 and OKGIIK_00415 are separated by 2 nucleotides OKGIIK_00415 and OKGIIK_00420 are separated by 28 nucleotides
OKGIIK_00395: OKGIIK_00395 - Peptidoglycan-binding protein, at 95,854 to 97,518
_00395
OKGIIK_00400: OKGIIK_00400 - hypothetical protein, at 97,789 to 98,034
_00400
OKGIIK_00405: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 98,029 to 98,604
tsaA
OKGIIK_00410: OKGIIK_00410 - YCII domain-containing protein, at 98,663 to 99,037
_00410
OKGIIK_00415: OKGIIK_00415 - hypothetical protein, at 99,040 to 99,561
_00415
OKGIIK_00420: OKGIIK_00420 - haloacid dehalogenase, at 99,590 to 100,306
_00420
Position (kb)
98
99 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 97.162 kb on + strand, within OKGIIK_00395 at 97.162 kb on + strand, within OKGIIK_00395 at 97.162 kb on + strand, within OKGIIK_00395 at 97.162 kb on + strand, within OKGIIK_00395 at 97.163 kb on - strand, within OKGIIK_00395 at 97.171 kb on + strand, within OKGIIK_00395 at 97.171 kb on + strand, within OKGIIK_00395 at 97.171 kb on + strand, within OKGIIK_00395 at 97.172 kb on - strand, within OKGIIK_00395 at 97.172 kb on - strand, within OKGIIK_00395 at 97.172 kb on - strand, within OKGIIK_00395 at 97.172 kb on - strand, within OKGIIK_00395 at 97.172 kb on - strand, within OKGIIK_00395 at 97.172 kb on - strand, within OKGIIK_00395 at 97.381 kb on + strand at 97.381 kb on + strand at 97.382 kb on - strand at 97.382 kb on - strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.435 kb on + strand at 97.436 kb on - strand at 97.436 kb on - strand at 97.436 kb on - strand at 97.436 kb on - strand at 97.436 kb on - strand at 97.436 kb on - strand at 97.436 kb on - strand at 97.957 kb on + strand, within OKGIIK_00400 at 98.162 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.171 kb on + strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.172 kb on - strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.528 kb on + strand, within tsaA at 98.529 kb on - strand, within tsaA at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.577 kb on + strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.606 kb on - strand at 98.606 kb on - strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.684 kb on + strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.685 kb on - strand at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.891 kb on + strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 98.892 kb on - strand, within OKGIIK_00410 at 99.404 kb on - strand, within OKGIIK_00415 at 99.404 kb on - strand, within OKGIIK_00415 at 99.427 kb on + strand, within OKGIIK_00415 at 99.428 kb on - strand, within OKGIIK_00415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 97,162 + OKGIIK_00395 0.79 -1.0 97,162 + OKGIIK_00395 0.79 -1.0 97,162 + OKGIIK_00395 0.79 -0.1 97,162 + OKGIIK_00395 0.79 +1.0 97,163 - OKGIIK_00395 0.79 -1.2 97,171 + OKGIIK_00395 0.79 +0.9 97,171 + OKGIIK_00395 0.79 -0.4 97,171 + OKGIIK_00395 0.79 -1.4 97,172 - OKGIIK_00395 0.79 +1.4 97,172 - OKGIIK_00395 0.79 +0.2 97,172 - OKGIIK_00395 0.79 -0.0 97,172 - OKGIIK_00395 0.79 +1.0 97,172 - OKGIIK_00395 0.79 +1.0 97,172 - OKGIIK_00395 0.79 +1.0 97,381 + +1.0 97,381 + +0.3 97,382 - -0.4 97,382 - -0.2 97,435 + -0.6 97,435 + -0.2 97,435 + +0.4 97,435 + -0.5 97,435 + +0.3 97,435 + +0.2 97,435 + +0.3 97,435 + -1.9 97,435 + +0.2 97,435 + -1.2 97,436 - -0.9 97,436 - +0.4 97,436 - +0.1 97,436 - +1.7 97,436 - -0.0 97,436 - -1.0 97,436 - -0.1 97,957 + OKGIIK_00400 0.68 +1.4 98,162 + tsaA OKGIIK_00405 0.23 +0.1 98,171 + tsaA OKGIIK_00405 0.25 -1.0 98,171 + tsaA OKGIIK_00405 0.25 -0.3 98,171 + tsaA OKGIIK_00405 0.25 +0.6 98,171 + tsaA OKGIIK_00405 0.25 -1.4 98,171 + tsaA OKGIIK_00405 0.25 +0.1 98,171 + tsaA OKGIIK_00405 0.25 +1.4 98,171 + tsaA OKGIIK_00405 0.25 +0.1 98,171 + tsaA OKGIIK_00405 0.25 +2.3 98,171 + tsaA OKGIIK_00405 0.25 -0.0 98,171 + tsaA OKGIIK_00405 0.25 -1.0 98,171 + tsaA OKGIIK_00405 0.25 -1.0 98,171 + tsaA OKGIIK_00405 0.25 +1.0 98,171 + tsaA OKGIIK_00405 0.25 +0.0 98,171 + tsaA OKGIIK_00405 0.25 -1.0 98,172 - tsaA OKGIIK_00405 0.25 +1.0 98,172 - tsaA OKGIIK_00405 0.25 -0.5 98,172 - tsaA OKGIIK_00405 0.25 +0.1 98,172 - tsaA OKGIIK_00405 0.25 -1.9 98,172 - tsaA OKGIIK_00405 0.25 +0.3 98,172 - tsaA OKGIIK_00405 0.25 -0.6 98,172 - tsaA OKGIIK_00405 0.25 +0.5 98,172 - tsaA OKGIIK_00405 0.25 +0.2 98,528 + tsaA OKGIIK_00405 0.87 -0.0 98,528 + tsaA OKGIIK_00405 0.87 +0.3 98,528 + tsaA OKGIIK_00405 0.87 +2.7 98,529 - tsaA OKGIIK_00405 0.87 +1.1 98,577 + -0.4 98,577 + +1.5 98,577 + -0.0 98,577 + -0.2 98,577 + -0.6 98,577 + +0.7 98,578 - +1.4 98,578 - +0.4 98,578 - -0.6 98,578 - -1.6 98,578 - -1.7 98,578 - +0.7 98,578 - +2.5 98,606 - -1.1 98,606 - +0.4 98,684 + -0.2 98,684 + -0.6 98,684 + +0.1 98,684 + -0.2 98,684 + +0.5 98,684 + -0.4 98,684 + -0.9 98,684 + +0.3 98,685 - -1.7 98,685 - -2.0 98,685 - -0.8 98,685 - +0.1 98,685 - +1.4 98,685 - -2.0 98,685 - -0.0 98,685 - -1.0 98,891 + OKGIIK_00410 0.61 -0.6 98,891 + OKGIIK_00410 0.61 +0.6 98,891 + OKGIIK_00410 0.61 +0.7 98,891 + OKGIIK_00410 0.61 +0.4 98,891 + OKGIIK_00410 0.61 -0.3 98,891 + OKGIIK_00410 0.61 +0.2 98,891 + OKGIIK_00410 0.61 +0.7 98,892 - OKGIIK_00410 0.61 +0.7 98,892 - OKGIIK_00410 0.61 -0.5 98,892 - OKGIIK_00410 0.61 -1.2 98,892 - OKGIIK_00410 0.61 +0.3 98,892 - OKGIIK_00410 0.61 +2.0 99,404 - OKGIIK_00415 0.70 -0.9 99,404 - OKGIIK_00415 0.70 -0.0 99,427 + OKGIIK_00415 0.74 -0.6 99,428 - OKGIIK_00415 0.74 -0.4
Or see this region's nucleotide sequence