Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00090 and ybeD overlap by 1 nucleotides ybeD and lipB are separated by 100 nucleotides lipB and lipA are separated by 9 nucleotides
OKGIIK_00090: OKGIIK_00090 - serine-type D-Ala-D-Ala carboxypeptidase, at 20,949 to 22,202
_00090
OKGIIK_00095: ybeD - DUF493 domain-containing protein, at 22,202 to 22,492
ybeD
OKGIIK_00100: lipB - lipoyl(octanoyl) transferase LipB, at 22,593 to 23,291
lipB
OKGIIK_00105: lipA - lipoyl synthase, at 23,301 to 24,311
lipA
Position (kb)
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 22.348 kb on - strand, within ybeD at 22.437 kb on - strand, within ybeD at 22.437 kb on - strand, within ybeD at 22.437 kb on - strand, within ybeD at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.546 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.580 kb on + strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.581 kb on - strand at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.848 kb on + strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 22.849 kb on - strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.007 kb on + strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.008 kb on - strand, within lipB at 23.130 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.158 kb on + strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.159 kb on - strand, within lipB at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.298 kb on + strand at 23.299 kb on - strand at 23.299 kb on - strand at 23.299 kb on - strand at 23.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 22,348 - ybeD OKGIIK_00095 0.50 +0.2 22,437 - ybeD OKGIIK_00095 0.81 -0.4 22,437 - ybeD OKGIIK_00095 0.81 -0.7 22,437 - ybeD OKGIIK_00095 0.81 -1.8 22,546 + -0.2 22,546 + -2.0 22,546 + +0.6 22,546 + -0.3 22,580 + -0.6 22,580 + +0.5 22,580 + +0.2 22,580 + -0.5 22,580 + +2.9 22,580 + +0.1 22,580 + -0.6 22,580 + -0.9 22,580 + -0.3 22,580 + +1.2 22,580 + +1.6 22,580 + +0.9 22,580 + +0.9 22,580 + +0.3 22,580 + -0.1 22,580 + +0.9 22,581 - +1.4 22,581 - +0.4 22,581 - +0.7 22,581 - +0.3 22,581 - -0.8 22,581 - -0.9 22,848 + lipB OKGIIK_00100 0.36 +1.2 22,848 + lipB OKGIIK_00100 0.36 -1.5 22,848 + lipB OKGIIK_00100 0.36 +1.2 22,848 + lipB OKGIIK_00100 0.36 +0.8 22,848 + lipB OKGIIK_00100 0.36 +0.6 22,848 + lipB OKGIIK_00100 0.36 -0.9 22,848 + lipB OKGIIK_00100 0.36 -1.3 22,849 - lipB OKGIIK_00100 0.37 -1.8 22,849 - lipB OKGIIK_00100 0.37 -1.3 22,849 - lipB OKGIIK_00100 0.37 -0.8 23,007 + lipB OKGIIK_00100 0.59 -1.0 23,007 + lipB OKGIIK_00100 0.59 +1.0 23,007 + lipB OKGIIK_00100 0.59 -1.0 23,007 + lipB OKGIIK_00100 0.59 +0.3 23,007 + lipB OKGIIK_00100 0.59 +0.1 23,007 + lipB OKGIIK_00100 0.59 -1.3 23,007 + lipB OKGIIK_00100 0.59 +0.6 23,007 + lipB OKGIIK_00100 0.59 -1.0 23,007 + lipB OKGIIK_00100 0.59 -1.0 23,007 + lipB OKGIIK_00100 0.59 +1.0 23,007 + lipB OKGIIK_00100 0.59 -0.0 23,007 + lipB OKGIIK_00100 0.59 -0.6 23,007 + lipB OKGIIK_00100 0.59 +0.1 23,008 - lipB OKGIIK_00100 0.59 -1.8 23,008 - lipB OKGIIK_00100 0.59 +0.8 23,008 - lipB OKGIIK_00100 0.59 +0.4 23,008 - lipB OKGIIK_00100 0.59 -0.5 23,008 - lipB OKGIIK_00100 0.59 -0.7 23,130 + lipB OKGIIK_00100 0.77 -1.7 23,158 + lipB OKGIIK_00100 0.81 -0.4 23,158 + lipB OKGIIK_00100 0.81 -0.6 23,158 + lipB OKGIIK_00100 0.81 -0.8 23,159 - lipB OKGIIK_00100 0.81 +1.1 23,159 - lipB OKGIIK_00100 0.81 +0.7 23,159 - lipB OKGIIK_00100 0.81 -1.7 23,159 - lipB OKGIIK_00100 0.81 -1.2 23,298 + -1.3 23,298 + +0.4 23,298 + +1.2 23,298 + +1.4 23,298 + +0.4 23,298 + +1.1 23,298 + +0.1 23,298 + +1.3 23,298 + -1.7 23,298 + -1.4 23,298 + -0.0 23,298 + +1.0 23,298 + +1.4 23,298 + +0.8 23,298 + -0.6 23,298 + +0.2 23,298 + +0.2 23,298 + +0.7 23,298 + +0.3 23,298 + -2.9 23,299 - +0.6 23,299 - +2.0 23,299 - +0.3 23,299 - -2.2
Or see this region's nucleotide sequence