Experiment: NL-CCM, start OD=0.3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_05333 and MPMX19_05334 are separated by 161 nucleotides MPMX19_05334 and MPMX19_05335 overlap by 4 nucleotides
MPMX19_05333: MPMX19_05333 - Thymidine phosphorylase, at 1,185 to 2,675
_05333
MPMX19_05334: MPMX19_05334 - hypothetical protein, at 2,837 to 4,468
_05334
MPMX19_05335: MPMX19_05335 - hypothetical protein, at 4,465 to 5,736
_05335
Position (kb)
2
3
4
5 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2.481 kb on + strand, within MPMX19_05333 at 2.496 kb on - strand, within MPMX19_05333 at 2.803 kb on + strand at 2.987 kb on + strand at 2.987 kb on + strand at 2.987 kb on + strand at 2.987 kb on + strand at 2.987 kb on + strand at 2.988 kb on - strand at 2.988 kb on - strand at 2.988 kb on - strand at 2.988 kb on - strand at 2.988 kb on - strand at 3.104 kb on + strand, within MPMX19_05334 at 3.104 kb on + strand, within MPMX19_05334 at 3.104 kb on + strand, within MPMX19_05334 at 3.105 kb on - strand, within MPMX19_05334 at 3.105 kb on - strand, within MPMX19_05334 at 3.105 kb on - strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.137 kb on + strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.138 kb on - strand, within MPMX19_05334 at 3.158 kb on + strand, within MPMX19_05334 at 3.158 kb on + strand, within MPMX19_05334 at 3.158 kb on + strand, within MPMX19_05334 at 3.158 kb on + strand, within MPMX19_05334 at 3.158 kb on + strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.159 kb on - strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.233 kb on + strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.234 kb on - strand, within MPMX19_05334 at 3.284 kb on + strand, within MPMX19_05334 at 3.284 kb on + strand, within MPMX19_05334 at 3.284 kb on + strand, within MPMX19_05334 at 3.284 kb on + strand, within MPMX19_05334 at 3.284 kb on + strand, within MPMX19_05334 at 3.285 kb on - strand, within MPMX19_05334 at 3.285 kb on - strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.512 kb on + strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.513 kb on - strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.650 kb on + strand, within MPMX19_05334 at 3.651 kb on - strand, within MPMX19_05334 at 3.651 kb on - strand, within MPMX19_05334 at 3.651 kb on - strand, within MPMX19_05334 at 3.651 kb on - strand, within MPMX19_05334 at 3.956 kb on + strand, within MPMX19_05334 at 4.064 kb on + strand, within MPMX19_05334 at 4.064 kb on + strand, within MPMX19_05334 at 4.064 kb on + strand, within MPMX19_05334 at 4.064 kb on + strand, within MPMX19_05334 at 4.064 kb on + strand, within MPMX19_05334 at 4.065 kb on - strand, within MPMX19_05334 at 4.065 kb on - strand, within MPMX19_05334 at 4.334 kb on + strand at 4.334 kb on + strand at 4.334 kb on + strand at 4.334 kb on + strand at 4.335 kb on - strand at 4.335 kb on - strand at 4.531 kb on + strand at 4.531 kb on + strand at 4.532 kb on - strand at 4.532 kb on - strand at 4.532 kb on - strand at 4.627 kb on + strand, within MPMX19_05335 at 4.627 kb on + strand, within MPMX19_05335 at 4.627 kb on + strand, within MPMX19_05335 at 4.627 kb on + strand, within MPMX19_05335 at 4.627 kb on + strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 4.628 kb on - strand, within MPMX19_05335 at 5.054 kb on - strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.128 kb on + strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.129 kb on - strand, within MPMX19_05335 at 5.350 kb on + strand, within MPMX19_05335 at 5.350 kb on + strand, within MPMX19_05335 at 5.350 kb on + strand, within MPMX19_05335 at 5.351 kb on - strand, within MPMX19_05335 at 5.351 kb on - strand, within MPMX19_05335 at 5.351 kb on - strand, within MPMX19_05335 at 5.351 kb on - strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.389 kb on + strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335 at 5.390 kb on - strand, within MPMX19_05335
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM, start OD=0.3 remove 2,481 + MPMX19_05333 0.87 -0.9 2,496 - MPMX19_05333 0.88 -0.7 2,803 + -0.7 2,987 + +0.5 2,987 + +0.1 2,987 + +0.0 2,987 + -0.6 2,987 + -0.8 2,988 - -1.1 2,988 - +0.8 2,988 - -0.5 2,988 - -0.1 2,988 - +0.3 3,104 + MPMX19_05334 0.16 -1.5 3,104 + MPMX19_05334 0.16 +1.7 3,104 + MPMX19_05334 0.16 -1.0 3,105 - MPMX19_05334 0.16 -1.0 3,105 - MPMX19_05334 0.16 +0.5 3,105 - MPMX19_05334 0.16 -0.6 3,137 + MPMX19_05334 0.18 -0.1 3,137 + MPMX19_05334 0.18 -0.7 3,137 + MPMX19_05334 0.18 -1.3 3,137 + MPMX19_05334 0.18 -0.9 3,137 + MPMX19_05334 0.18 +1.0 3,137 + MPMX19_05334 0.18 -0.5 3,137 + MPMX19_05334 0.18 +0.2 3,137 + MPMX19_05334 0.18 -0.5 3,137 + MPMX19_05334 0.18 -0.5 3,137 + MPMX19_05334 0.18 +1.0 3,137 + MPMX19_05334 0.18 -0.2 3,137 + MPMX19_05334 0.18 +0.3 3,137 + MPMX19_05334 0.18 +1.4 3,137 + MPMX19_05334 0.18 -0.1 3,137 + MPMX19_05334 0.18 -1.8 3,138 - MPMX19_05334 0.18 -1.6 3,138 - MPMX19_05334 0.18 +0.1 3,138 - MPMX19_05334 0.18 -1.3 3,138 - MPMX19_05334 0.18 -0.9 3,138 - MPMX19_05334 0.18 -1.2 3,138 - MPMX19_05334 0.18 +1.1 3,138 - MPMX19_05334 0.18 -0.8 3,138 - MPMX19_05334 0.18 +0.1 3,138 - MPMX19_05334 0.18 -1.1 3,138 - MPMX19_05334 0.18 -1.3 3,138 - MPMX19_05334 0.18 +0.4 3,138 - MPMX19_05334 0.18 -1.3 3,138 - MPMX19_05334 0.18 -0.9 3,138 - MPMX19_05334 0.18 +1.2 3,138 - MPMX19_05334 0.18 +0.0 3,138 - MPMX19_05334 0.18 -0.2 3,138 - MPMX19_05334 0.18 -2.3 3,138 - MPMX19_05334 0.18 -1.8 3,158 + MPMX19_05334 0.20 -1.4 3,158 + MPMX19_05334 0.20 +0.1 3,158 + MPMX19_05334 0.20 -0.3 3,158 + MPMX19_05334 0.20 +0.1 3,158 + MPMX19_05334 0.20 -0.9 3,159 - MPMX19_05334 0.20 -0.5 3,159 - MPMX19_05334 0.20 -1.7 3,159 - MPMX19_05334 0.20 -0.7 3,159 - MPMX19_05334 0.20 -0.3 3,159 - MPMX19_05334 0.20 +0.2 3,159 - MPMX19_05334 0.20 -0.6 3,159 - MPMX19_05334 0.20 -0.4 3,159 - MPMX19_05334 0.20 +1.3 3,159 - MPMX19_05334 0.20 -1.2 3,159 - MPMX19_05334 0.20 -1.6 3,159 - MPMX19_05334 0.20 -1.0 3,159 - MPMX19_05334 0.20 -1.2 3,233 + MPMX19_05334 0.24 +1.3 3,233 + MPMX19_05334 0.24 -1.7 3,233 + MPMX19_05334 0.24 -0.2 3,233 + MPMX19_05334 0.24 -0.3 3,233 + MPMX19_05334 0.24 -0.5 3,233 + MPMX19_05334 0.24 -1.5 3,233 + MPMX19_05334 0.24 -0.9 3,233 + MPMX19_05334 0.24 -2.0 3,233 + MPMX19_05334 0.24 +0.3 3,234 - MPMX19_05334 0.24 -0.5 3,234 - MPMX19_05334 0.24 -1.3 3,234 - MPMX19_05334 0.24 -0.8 3,234 - MPMX19_05334 0.24 -0.7 3,234 - MPMX19_05334 0.24 +0.7 3,234 - MPMX19_05334 0.24 -2.1 3,234 - MPMX19_05334 0.24 -0.2 3,234 - MPMX19_05334 0.24 +0.2 3,284 + MPMX19_05334 0.27 +0.0 3,284 + MPMX19_05334 0.27 -1.1 3,284 + MPMX19_05334 0.27 -1.1 3,284 + MPMX19_05334 0.27 -0.6 3,284 + MPMX19_05334 0.27 -0.7 3,285 - MPMX19_05334 0.27 -1.1 3,285 - MPMX19_05334 0.27 -1.2 3,512 + MPMX19_05334 0.41 +0.3 3,512 + MPMX19_05334 0.41 -1.2 3,512 + MPMX19_05334 0.41 -0.5 3,512 + MPMX19_05334 0.41 -0.5 3,512 + MPMX19_05334 0.41 -1.2 3,512 + MPMX19_05334 0.41 -0.9 3,512 + MPMX19_05334 0.41 -1.4 3,512 + MPMX19_05334 0.41 -0.9 3,513 - MPMX19_05334 0.41 +0.7 3,513 - MPMX19_05334 0.41 -1.4 3,513 - MPMX19_05334 0.41 -0.8 3,513 - MPMX19_05334 0.41 -0.9 3,513 - MPMX19_05334 0.41 -1.3 3,513 - MPMX19_05334 0.41 +2.1 3,513 - MPMX19_05334 0.41 +0.1 3,513 - MPMX19_05334 0.41 +0.9 3,650 + MPMX19_05334 0.50 -2.0 3,650 + MPMX19_05334 0.50 -1.5 3,650 + MPMX19_05334 0.50 -0.2 3,650 + MPMX19_05334 0.50 -0.4 3,650 + MPMX19_05334 0.50 -0.3 3,650 + MPMX19_05334 0.50 -1.1 3,650 + MPMX19_05334 0.50 -0.7 3,651 - MPMX19_05334 0.50 +0.8 3,651 - MPMX19_05334 0.50 -0.7 3,651 - MPMX19_05334 0.50 -0.1 3,651 - MPMX19_05334 0.50 +0.3 3,956 + MPMX19_05334 0.69 -1.2 4,064 + MPMX19_05334 0.75 -0.7 4,064 + MPMX19_05334 0.75 -2.1 4,064 + MPMX19_05334 0.75 -1.7 4,064 + MPMX19_05334 0.75 -1.1 4,064 + MPMX19_05334 0.75 +0.5 4,065 - MPMX19_05334 0.75 -1.7 4,065 - MPMX19_05334 0.75 -1.3 4,334 + -0.6 4,334 + -1.2 4,334 + -0.5 4,334 + +0.3 4,335 - +0.3 4,335 - -2.2 4,531 + +1.1 4,531 + +0.5 4,532 - +0.1 4,532 - -0.7 4,532 - +0.4 4,627 + MPMX19_05335 0.13 -2.3 4,627 + MPMX19_05335 0.13 -0.7 4,627 + MPMX19_05335 0.13 -2.8 4,627 + MPMX19_05335 0.13 -0.9 4,627 + MPMX19_05335 0.13 -0.6 4,628 - MPMX19_05335 0.13 -0.3 4,628 - MPMX19_05335 0.13 -0.5 4,628 - MPMX19_05335 0.13 -1.3 4,628 - MPMX19_05335 0.13 -0.7 4,628 - MPMX19_05335 0.13 +0.1 4,628 - MPMX19_05335 0.13 -0.6 4,628 - MPMX19_05335 0.13 +0.2 4,628 - MPMX19_05335 0.13 -1.6 5,054 - MPMX19_05335 0.46 +0.9 5,128 + MPMX19_05335 0.52 -0.5 5,128 + MPMX19_05335 0.52 -1.5 5,128 + MPMX19_05335 0.52 -1.9 5,128 + MPMX19_05335 0.52 +0.3 5,128 + MPMX19_05335 0.52 +0.9 5,128 + MPMX19_05335 0.52 -0.4 5,128 + MPMX19_05335 0.52 -1.3 5,128 + MPMX19_05335 0.52 +0.7 5,128 + MPMX19_05335 0.52 -0.9 5,128 + MPMX19_05335 0.52 -0.1 5,128 + MPMX19_05335 0.52 -0.5 5,128 + MPMX19_05335 0.52 -1.1 5,128 + MPMX19_05335 0.52 -0.7 5,128 + MPMX19_05335 0.52 -0.1 5,128 + MPMX19_05335 0.52 -1.2 5,128 + MPMX19_05335 0.52 -0.8 5,128 + MPMX19_05335 0.52 +0.8 5,128 + MPMX19_05335 0.52 +0.2 5,128 + MPMX19_05335 0.52 -0.3 5,129 - MPMX19_05335 0.52 +0.2 5,129 - MPMX19_05335 0.52 -0.6 5,129 - MPMX19_05335 0.52 -0.9 5,129 - MPMX19_05335 0.52 -0.2 5,129 - MPMX19_05335 0.52 -0.3 5,129 - MPMX19_05335 0.52 -0.7 5,129 - MPMX19_05335 0.52 -1.3 5,129 - MPMX19_05335 0.52 -0.7 5,129 - MPMX19_05335 0.52 -1.3 5,129 - MPMX19_05335 0.52 -1.6 5,129 - MPMX19_05335 0.52 +0.2 5,129 - MPMX19_05335 0.52 -1.5 5,129 - MPMX19_05335 0.52 +1.4 5,129 - MPMX19_05335 0.52 -1.9 5,129 - MPMX19_05335 0.52 -0.1 5,129 - MPMX19_05335 0.52 +0.4 5,129 - MPMX19_05335 0.52 -0.7 5,350 + MPMX19_05335 0.70 -0.3 5,350 + MPMX19_05335 0.70 -0.5 5,350 + MPMX19_05335 0.70 -2.2 5,351 - MPMX19_05335 0.70 -1.2 5,351 - MPMX19_05335 0.70 -1.4 5,351 - MPMX19_05335 0.70 -1.1 5,351 - MPMX19_05335 0.70 -0.4 5,389 + MPMX19_05335 0.73 +0.5 5,389 + MPMX19_05335 0.73 -0.7 5,389 + MPMX19_05335 0.73 -0.3 5,389 + MPMX19_05335 0.73 +1.4 5,389 + MPMX19_05335 0.73 -1.1 5,389 + MPMX19_05335 0.73 -1.2 5,389 + MPMX19_05335 0.73 -0.1 5,389 + MPMX19_05335 0.73 +0.4 5,389 + MPMX19_05335 0.73 +0.3 5,389 + MPMX19_05335 0.73 -0.1 5,389 + MPMX19_05335 0.73 -0.5 5,389 + MPMX19_05335 0.73 +0.6 5,390 - MPMX19_05335 0.73 +0.3 5,390 - MPMX19_05335 0.73 -1.4 5,390 - MPMX19_05335 0.73 -1.3 5,390 - MPMX19_05335 0.73 -1.1 5,390 - MPMX19_05335 0.73 -0.1 5,390 - MPMX19_05335 0.73 -1.1 5,390 - MPMX19_05335 0.73 -0.7 5,390 - MPMX19_05335 0.73 -0.9 5,390 - MPMX19_05335 0.73 -0.9 5,390 - MPMX19_05335 0.73 +0.7 5,390 - MPMX19_05335 0.73 -0.4 5,390 - MPMX19_05335 0.73 +0.1 5,390 - MPMX19_05335 0.73 -0.7 5,390 - MPMX19_05335 0.73 -1.3 5,390 - MPMX19_05335 0.73 -1.2 5,390 - MPMX19_05335 0.73 -0.4
Or see this region's nucleotide sequence