Experiment: NL-CCM, start OD=0.3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_03795 and MPMX19_03796 are separated by 304 nucleotides MPMX19_03796 and MPMX19_03797 overlap by 29 nucleotides
MPMX19_03795: MPMX19_03795 - hypothetical protein, at 29,546 to 31,087
_03795
MPMX19_03796: MPMX19_03796 - hypothetical protein, at 31,392 to 32,138
_03796
MPMX19_03797: MPMX19_03797 - hypothetical protein, at 32,110 to 33,336
_03797
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 30.401 kb on + strand, within MPMX19_03795 at 30.401 kb on + strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 30.402 kb on - strand, within MPMX19_03795 at 31.217 kb on + strand at 31.217 kb on + strand at 31.218 kb on - strand at 31.218 kb on - strand at 31.218 kb on - strand at 31.224 kb on + strand at 31.224 kb on + strand at 31.446 kb on + strand at 31.446 kb on + strand at 31.447 kb on - strand at 31.447 kb on - strand at 31.452 kb on + strand at 31.452 kb on + strand at 31.453 kb on - strand at 31.453 kb on - strand at 31.557 kb on + strand, within MPMX19_03796 at 31.557 kb on + strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.558 kb on - strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.584 kb on + strand, within MPMX19_03796 at 31.585 kb on - strand, within MPMX19_03796 at 31.585 kb on - strand, within MPMX19_03796 at 31.585 kb on - strand, within MPMX19_03796 at 31.585 kb on - strand, within MPMX19_03796 at 31.585 kb on - strand, within MPMX19_03796 at 31.585 kb on - strand, within MPMX19_03796 at 31.602 kb on + strand, within MPMX19_03796 at 31.602 kb on + strand, within MPMX19_03796 at 31.602 kb on + strand, within MPMX19_03796 at 31.603 kb on - strand, within MPMX19_03796 at 31.603 kb on - strand, within MPMX19_03796 at 31.603 kb on - strand, within MPMX19_03796 at 31.603 kb on - strand, within MPMX19_03796 at 31.603 kb on - strand, within MPMX19_03796 at 31.603 kb on - strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.767 kb on + strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.768 kb on - strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.779 kb on + strand, within MPMX19_03796 at 31.780 kb on - strand, within MPMX19_03796 at 31.780 kb on - strand, within MPMX19_03796 at 31.780 kb on - strand, within MPMX19_03796 at 31.780 kb on - strand, within MPMX19_03796 at 31.780 kb on - strand, within MPMX19_03796 at 31.857 kb on + strand, within MPMX19_03796 at 31.857 kb on + strand, within MPMX19_03796 at 31.858 kb on - strand, within MPMX19_03796 at 31.858 kb on - strand, within MPMX19_03796 at 31.858 kb on - strand, within MPMX19_03796 at 31.878 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.905 kb on + strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.906 kb on - strand, within MPMX19_03796 at 31.926 kb on + strand, within MPMX19_03796 at 31.926 kb on + strand, within MPMX19_03796 at 31.926 kb on + strand, within MPMX19_03796 at 31.927 kb on - strand, within MPMX19_03796 at 31.927 kb on - strand, within MPMX19_03796 at 31.927 kb on - strand, within MPMX19_03796 at 31.927 kb on - strand, within MPMX19_03796 at 32.031 kb on + strand, within MPMX19_03796 at 32.031 kb on + strand, within MPMX19_03796 at 32.032 kb on - strand, within MPMX19_03796 at 32.088 kb on + strand at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.362 kb on + strand, within MPMX19_03797 at 32.363 kb on - strand, within MPMX19_03797 at 32.363 kb on - strand, within MPMX19_03797 at 32.363 kb on - strand, within MPMX19_03797 at 32.363 kb on - strand, within MPMX19_03797 at 32.363 kb on - strand, within MPMX19_03797 at 32.363 kb on - strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.398 kb on + strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.399 kb on - strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.473 kb on + strand, within MPMX19_03797 at 32.474 kb on - strand, within MPMX19_03797 at 32.474 kb on - strand, within MPMX19_03797 at 32.474 kb on - strand, within MPMX19_03797 at 32.474 kb on - strand, within MPMX19_03797 at 32.474 kb on - strand, within MPMX19_03797 at 32.761 kb on + strand, within MPMX19_03797 at 32.761 kb on + strand, within MPMX19_03797 at 32.761 kb on + strand, within MPMX19_03797 at 32.761 kb on + strand, within MPMX19_03797 at 32.762 kb on - strand, within MPMX19_03797 at 32.762 kb on - strand, within MPMX19_03797 at 32.762 kb on - strand, within MPMX19_03797 at 32.762 kb on - strand, within MPMX19_03797 at 32.783 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.827 kb on + strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.828 kb on - strand, within MPMX19_03797 at 32.864 kb on + strand, within MPMX19_03797 at 32.864 kb on + strand, within MPMX19_03797 at 32.864 kb on + strand, within MPMX19_03797 at 32.865 kb on - strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.932 kb on + strand, within MPMX19_03797 at 32.933 kb on - strand, within MPMX19_03797 at 32.933 kb on - strand, within MPMX19_03797 at 32.933 kb on - strand, within MPMX19_03797 at 32.933 kb on - strand, within MPMX19_03797 at 32.933 kb on - strand, within MPMX19_03797 at 32.933 kb on - strand, within MPMX19_03797 at 33.003 kb on + strand, within MPMX19_03797 at 33.003 kb on + strand, within MPMX19_03797 at 33.004 kb on - strand, within MPMX19_03797 at 33.011 kb on + strand, within MPMX19_03797 at 33.082 kb on + strand, within MPMX19_03797 at 33.082 kb on + strand, within MPMX19_03797 at 33.083 kb on - strand, within MPMX19_03797 at 33.083 kb on - strand, within MPMX19_03797
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM, start OD=0.3 remove 30,401 + MPMX19_03795 0.55 -0.6 30,401 + MPMX19_03795 0.55 +0.8 30,402 - MPMX19_03795 0.56 +0.5 30,402 - MPMX19_03795 0.56 +0.9 30,402 - MPMX19_03795 0.56 +0.2 30,402 - MPMX19_03795 0.56 -0.3 30,402 - MPMX19_03795 0.56 +0.2 30,402 - MPMX19_03795 0.56 +0.8 30,402 - MPMX19_03795 0.56 +1.0 30,402 - MPMX19_03795 0.56 +0.4 30,402 - MPMX19_03795 0.56 +0.0 31,217 + -0.2 31,217 + +2.2 31,218 - -0.2 31,218 - -0.5 31,218 - -0.3 31,224 + -0.0 31,224 + -0.5 31,446 + -0.5 31,446 + -0.1 31,447 - -0.1 31,447 - -1.4 31,452 + -1.0 31,452 + -0.1 31,453 - +0.2 31,453 - -1.6 31,557 + MPMX19_03796 0.22 -0.6 31,557 + MPMX19_03796 0.22 +0.0 31,558 - MPMX19_03796 0.22 -1.6 31,558 - MPMX19_03796 0.22 +2.2 31,558 - MPMX19_03796 0.22 +1.4 31,558 - MPMX19_03796 0.22 -1.4 31,558 - MPMX19_03796 0.22 -0.4 31,558 - MPMX19_03796 0.22 +0.2 31,558 - MPMX19_03796 0.22 +1.0 31,558 - MPMX19_03796 0.22 -0.4 31,584 + MPMX19_03796 0.26 -1.3 31,584 + MPMX19_03796 0.26 -0.3 31,584 + MPMX19_03796 0.26 -1.1 31,584 + MPMX19_03796 0.26 +1.0 31,584 + MPMX19_03796 0.26 +0.1 31,584 + MPMX19_03796 0.26 +0.8 31,584 + MPMX19_03796 0.26 -2.6 31,584 + MPMX19_03796 0.26 -1.4 31,585 - MPMX19_03796 0.26 -1.8 31,585 - MPMX19_03796 0.26 +0.0 31,585 - MPMX19_03796 0.26 -1.2 31,585 - MPMX19_03796 0.26 +0.5 31,585 - MPMX19_03796 0.26 -0.1 31,585 - MPMX19_03796 0.26 -0.8 31,602 + MPMX19_03796 0.28 -1.4 31,602 + MPMX19_03796 0.28 -0.6 31,602 + MPMX19_03796 0.28 -1.2 31,603 - MPMX19_03796 0.28 +0.3 31,603 - MPMX19_03796 0.28 +0.3 31,603 - MPMX19_03796 0.28 -0.3 31,603 - MPMX19_03796 0.28 +1.5 31,603 - MPMX19_03796 0.28 -0.9 31,603 - MPMX19_03796 0.28 -1.1 31,767 + MPMX19_03796 0.50 +1.4 31,767 + MPMX19_03796 0.50 -1.1 31,767 + MPMX19_03796 0.50 -2.0 31,767 + MPMX19_03796 0.50 -1.0 31,767 + MPMX19_03796 0.50 -0.5 31,767 + MPMX19_03796 0.50 -0.3 31,767 + MPMX19_03796 0.50 -1.8 31,767 + MPMX19_03796 0.50 +0.3 31,767 + MPMX19_03796 0.50 +0.0 31,767 + MPMX19_03796 0.50 -0.7 31,767 + MPMX19_03796 0.50 -0.9 31,767 + MPMX19_03796 0.50 -0.8 31,767 + MPMX19_03796 0.50 -1.9 31,767 + MPMX19_03796 0.50 -0.4 31,768 - MPMX19_03796 0.50 -1.2 31,768 - MPMX19_03796 0.50 -0.7 31,768 - MPMX19_03796 0.50 -0.8 31,768 - MPMX19_03796 0.50 -0.8 31,768 - MPMX19_03796 0.50 -0.6 31,768 - MPMX19_03796 0.50 +0.6 31,768 - MPMX19_03796 0.50 -2.5 31,768 - MPMX19_03796 0.50 -1.2 31,768 - MPMX19_03796 0.50 -0.7 31,768 - MPMX19_03796 0.50 -2.1 31,779 + MPMX19_03796 0.52 -1.7 31,779 + MPMX19_03796 0.52 -0.2 31,779 + MPMX19_03796 0.52 -0.6 31,779 + MPMX19_03796 0.52 -0.4 31,779 + MPMX19_03796 0.52 -1.3 31,779 + MPMX19_03796 0.52 +0.2 31,779 + MPMX19_03796 0.52 -1.6 31,779 + MPMX19_03796 0.52 -1.4 31,779 + MPMX19_03796 0.52 -1.5 31,780 - MPMX19_03796 0.52 -0.6 31,780 - MPMX19_03796 0.52 -1.1 31,780 - MPMX19_03796 0.52 +0.9 31,780 - MPMX19_03796 0.52 -0.3 31,780 - MPMX19_03796 0.52 -2.0 31,857 + MPMX19_03796 0.62 -1.5 31,857 + MPMX19_03796 0.62 -0.3 31,858 - MPMX19_03796 0.62 +0.6 31,858 - MPMX19_03796 0.62 -0.9 31,858 - MPMX19_03796 0.62 -1.4 31,878 + MPMX19_03796 0.65 +0.1 31,905 + MPMX19_03796 0.69 -0.5 31,905 + MPMX19_03796 0.69 +0.1 31,905 + MPMX19_03796 0.69 +1.5 31,905 + MPMX19_03796 0.69 -1.5 31,905 + MPMX19_03796 0.69 -0.2 31,905 + MPMX19_03796 0.69 -1.8 31,905 + MPMX19_03796 0.69 -1.1 31,906 - MPMX19_03796 0.69 -0.1 31,906 - MPMX19_03796 0.69 +0.6 31,906 - MPMX19_03796 0.69 +0.6 31,906 - MPMX19_03796 0.69 -0.1 31,906 - MPMX19_03796 0.69 -0.5 31,906 - MPMX19_03796 0.69 -2.5 31,906 - MPMX19_03796 0.69 -1.3 31,926 + MPMX19_03796 0.71 +1.0 31,926 + MPMX19_03796 0.71 -2.7 31,926 + MPMX19_03796 0.71 +0.2 31,927 - MPMX19_03796 0.72 +1.5 31,927 - MPMX19_03796 0.72 -1.0 31,927 - MPMX19_03796 0.72 -0.4 31,927 - MPMX19_03796 0.72 -0.7 32,031 + MPMX19_03796 0.86 +0.2 32,031 + MPMX19_03796 0.86 -0.6 32,032 - MPMX19_03796 0.86 -3.2 32,088 + -1.7 32,362 + MPMX19_03797 0.21 -1.3 32,362 + MPMX19_03797 0.21 -0.5 32,362 + MPMX19_03797 0.21 -0.8 32,362 + MPMX19_03797 0.21 +0.2 32,362 + MPMX19_03797 0.21 +1.2 32,362 + MPMX19_03797 0.21 +0.6 32,362 + MPMX19_03797 0.21 -0.6 32,362 + MPMX19_03797 0.21 -0.2 32,362 + MPMX19_03797 0.21 +0.0 32,363 - MPMX19_03797 0.21 -0.7 32,363 - MPMX19_03797 0.21 -0.3 32,363 - MPMX19_03797 0.21 -0.7 32,363 - MPMX19_03797 0.21 -0.8 32,363 - MPMX19_03797 0.21 +0.9 32,363 - MPMX19_03797 0.21 +0.1 32,398 + MPMX19_03797 0.23 +0.2 32,398 + MPMX19_03797 0.23 -0.1 32,398 + MPMX19_03797 0.23 -0.3 32,398 + MPMX19_03797 0.23 +0.5 32,398 + MPMX19_03797 0.23 -0.1 32,398 + MPMX19_03797 0.23 +0.4 32,398 + MPMX19_03797 0.23 -1.1 32,398 + MPMX19_03797 0.23 -1.1 32,399 - MPMX19_03797 0.24 -0.1 32,399 - MPMX19_03797 0.24 -0.7 32,399 - MPMX19_03797 0.24 -0.1 32,399 - MPMX19_03797 0.24 -0.9 32,399 - MPMX19_03797 0.24 +1.2 32,399 - MPMX19_03797 0.24 -0.6 32,399 - MPMX19_03797 0.24 -1.0 32,399 - MPMX19_03797 0.24 -0.2 32,473 + MPMX19_03797 0.30 +0.1 32,473 + MPMX19_03797 0.30 -1.4 32,473 + MPMX19_03797 0.30 -0.9 32,473 + MPMX19_03797 0.30 -1.5 32,473 + MPMX19_03797 0.30 -0.3 32,473 + MPMX19_03797 0.30 +0.8 32,473 + MPMX19_03797 0.30 -1.3 32,474 - MPMX19_03797 0.30 +1.2 32,474 - MPMX19_03797 0.30 -0.1 32,474 - MPMX19_03797 0.30 -0.6 32,474 - MPMX19_03797 0.30 -1.4 32,474 - MPMX19_03797 0.30 -1.1 32,761 + MPMX19_03797 0.53 +0.3 32,761 + MPMX19_03797 0.53 -0.1 32,761 + MPMX19_03797 0.53 -0.4 32,761 + MPMX19_03797 0.53 -1.1 32,762 - MPMX19_03797 0.53 -0.1 32,762 - MPMX19_03797 0.53 -2.2 32,762 - MPMX19_03797 0.53 -2.8 32,762 - MPMX19_03797 0.53 +0.9 32,783 + MPMX19_03797 0.55 -1.5 32,827 + MPMX19_03797 0.58 +0.4 32,827 + MPMX19_03797 0.58 -1.7 32,827 + MPMX19_03797 0.58 +0.4 32,827 + MPMX19_03797 0.58 -0.5 32,827 + MPMX19_03797 0.58 -0.4 32,827 + MPMX19_03797 0.58 +0.3 32,827 + MPMX19_03797 0.58 +1.2 32,828 - MPMX19_03797 0.59 -0.1 32,828 - MPMX19_03797 0.59 -0.7 32,828 - MPMX19_03797 0.59 -0.7 32,828 - MPMX19_03797 0.59 -0.5 32,828 - MPMX19_03797 0.59 +0.8 32,828 - MPMX19_03797 0.59 -0.9 32,828 - MPMX19_03797 0.59 +0.6 32,828 - MPMX19_03797 0.59 -0.0 32,864 + MPMX19_03797 0.61 -1.6 32,864 + MPMX19_03797 0.61 -0.1 32,864 + MPMX19_03797 0.61 +0.6 32,865 - MPMX19_03797 0.62 +1.5 32,932 + MPMX19_03797 0.67 -1.0 32,932 + MPMX19_03797 0.67 -1.0 32,932 + MPMX19_03797 0.67 -2.5 32,932 + MPMX19_03797 0.67 -0.5 32,932 + MPMX19_03797 0.67 -2.1 32,932 + MPMX19_03797 0.67 -0.5 32,932 + MPMX19_03797 0.67 -0.4 32,932 + MPMX19_03797 0.67 -2.5 32,933 - MPMX19_03797 0.67 +0.6 32,933 - MPMX19_03797 0.67 +0.3 32,933 - MPMX19_03797 0.67 -2.8 32,933 - MPMX19_03797 0.67 +0.6 32,933 - MPMX19_03797 0.67 +0.2 32,933 - MPMX19_03797 0.67 +0.9 33,003 + MPMX19_03797 0.73 -0.1 33,003 + MPMX19_03797 0.73 -1.0 33,004 - MPMX19_03797 0.73 -0.1 33,011 + MPMX19_03797 0.73 +0.0 33,082 + MPMX19_03797 0.79 -1.1 33,082 + MPMX19_03797 0.79 +0.2 33,083 - MPMX19_03797 0.79 +0.7 33,083 - MPMX19_03797 0.79 -0.5
Or see this region's nucleotide sequence