Experiment: NL-CCM, start OD=0.3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_02006 and MPMX19_02007 are separated by 47 nucleotides MPMX19_02007 and MPMX19_02008 are separated by 141 nucleotides MPMX19_02008 and MPMX19_02009 overlap by 4 nucleotides
MPMX19_02006: MPMX19_02006 - Methionyl-tRNA formyltransferase, at 2,094,827 to 2,095,783
_02006
MPMX19_02007: MPMX19_02007 - Peptide deformylase 1, at 2,095,831 to 2,096,343
_02007
MPMX19_02008: MPMX19_02008 - Adenosylcobinamide-GDP ribazoletransferase, at 2,096,485 to 2,097,321
_02008
MPMX19_02009: MPMX19_02009 - Adenosylcobalamin/alpha-ribazole phosphatase, at 2,097,318 to 2,097,962
_02009
Position (kb)
2095
2096
2097 Strain fitness (log2 ratio)
-2
-1
0
1 at 2095.793 kb on - strand at 2095.793 kb on - strand at 2095.793 kb on - strand at 2096.361 kb on - strand at 2096.386 kb on - strand at 2096.421 kb on + strand at 2096.421 kb on + strand at 2096.421 kb on + strand at 2096.421 kb on + strand at 2096.421 kb on + strand at 2096.421 kb on + strand at 2096.421 kb on + strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.422 kb on - strand at 2096.479 kb on + strand at 2096.479 kb on + strand at 2096.479 kb on + strand at 2096.479 kb on + strand at 2096.479 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM, start OD=0.3 remove 2,095,793 - -1.1 2,095,793 - +0.4 2,095,793 - +0.5 2,096,361 - +1.1 2,096,386 - -0.6 2,096,421 + -0.6 2,096,421 + -1.1 2,096,421 + -0.7 2,096,421 + -1.5 2,096,421 + -0.5 2,096,421 + +0.9 2,096,421 + -2.7 2,096,422 - -0.1 2,096,422 - -0.2 2,096,422 - +0.7 2,096,422 - -0.9 2,096,422 - -1.4 2,096,422 - +0.5 2,096,422 - -1.7 2,096,422 - -2.6 2,096,422 - +0.8 2,096,422 - +0.7 2,096,479 + -1.9 2,096,479 + -1.4 2,096,479 + +0.1 2,096,479 + -0.4 2,096,479 + -1.0
Or see this region's nucleotide sequence