Strain Fitness in Pseudomonas sp. DMC3 around GFF5250

Experiment: D-Glucose and D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5249 and GFF5250 are separated by 431 nucleotidesGFF5250 and GFF5251 are separated by 15 nucleotides GFF5249 - Nitrate transporter, at 29,998 to 31,209 GFF5249 GFF5250 - putative transcriptional regulatory protein pdtaR, at 31,641 to 32,216 GFF5250 GFF5251 - Nitrate/nitrite binding protein NrtA, at 32,232 to 33,443 GFF5251 Position (kb) 31 32 33Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 30.905 kb on + strand, within GFF5249at 30.906 kb on - strand, within GFF5249at 30.906 kb on - strand, within GFF5249at 30.906 kb on - strand, within GFF5249at 30.906 kb on - strand, within GFF5249at 31.151 kb on + strandat 31.151 kb on + strandat 31.152 kb on - strandat 31.366 kb on + strandat 31.367 kb on - strandat 31.367 kb on - strandat 31.402 kb on + strandat 31.406 kb on + strandat 31.451 kb on + strandat 31.452 kb on - strandat 31.533 kb on - strandat 31.730 kb on + strand, within GFF5250at 31.731 kb on - strand, within GFF5250at 31.731 kb on - strand, within GFF5250at 31.731 kb on - strand, within GFF5250at 31.731 kb on - strand, within GFF5250at 31.732 kb on + strand, within GFF5250at 31.732 kb on + strand, within GFF5250at 31.732 kb on + strand, within GFF5250at 31.733 kb on - strand, within GFF5250at 31.733 kb on - strand, within GFF5250at 31.733 kb on - strand, within GFF5250at 31.733 kb on - strand, within GFF5250at 31.733 kb on - strand, within GFF5250at 31.770 kb on + strand, within GFF5250at 31.770 kb on + strand, within GFF5250at 31.771 kb on - strand, within GFF5250at 31.921 kb on + strand, within GFF5250at 31.921 kb on + strand, within GFF5250at 31.921 kb on + strand, within GFF5250at 31.921 kb on + strand, within GFF5250at 31.922 kb on - strand, within GFF5250at 31.922 kb on - strand, within GFF5250at 31.922 kb on - strand, within GFF5250at 31.922 kb on - strand, within GFF5250at 32.294 kb on - strandat 32.373 kb on - strand, within GFF5251at 32.437 kb on + strand, within GFF5251at 32.438 kb on - strand, within GFF5251at 32.506 kb on + strand, within GFF5251at 32.585 kb on - strand, within GFF5251at 32.594 kb on + strand, within GFF5251at 32.594 kb on + strand, within GFF5251at 32.594 kb on + strand, within GFF5251at 32.594 kb on + strand, within GFF5251at 32.632 kb on + strand, within GFF5251at 32.633 kb on - strand, within GFF5251at 32.633 kb on - strand, within GFF5251at 32.633 kb on - strand, within GFF5251at 32.743 kb on + strand, within GFF5251at 32.980 kb on + strand, within GFF5251at 32.980 kb on + strand, within GFF5251at 32.981 kb on - strand, within GFF5251at 32.983 kb on + strand, within GFF5251at 32.984 kb on - strand, within GFF5251at 32.984 kb on - strand, within GFF5251at 33.183 kb on + strand, within GFF5251at 33.183 kb on + strand, within GFF5251at 33.183 kb on + strand, within GFF5251at 33.183 kb on + strand, within GFF5251at 33.184 kb on - strand, within GFF5251at 33.208 kb on + strand, within GFF5251at 33.209 kb on - strand, within GFF5251

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose and D,L-Lactate (C)
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30,905 + GFF5249 0.75 -1.7
30,906 - GFF5249 0.75 -2.5
30,906 - GFF5249 0.75 -3.8
30,906 - GFF5249 0.75 -1.4
30,906 - GFF5249 0.75 -3.1
31,151 + -1.3
31,151 + -1.9
31,152 - -3.8
31,366 + -2.5
31,367 - -0.5
31,367 - -0.1
31,402 + -1.1
31,406 + -2.8
31,451 + -0.0
31,452 - -0.7
31,533 - +0.7
31,730 + GFF5250 0.15 -0.4
31,731 - GFF5250 0.16 -2.1
31,731 - GFF5250 0.16 -0.7
31,731 - GFF5250 0.16 -2.7
31,731 - GFF5250 0.16 -0.2
31,732 + GFF5250 0.16 -3.8
31,732 + GFF5250 0.16 -1.0
31,732 + GFF5250 0.16 -2.3
31,733 - GFF5250 0.16 -2.4
31,733 - GFF5250 0.16 -3.0
31,733 - GFF5250 0.16 -2.1
31,733 - GFF5250 0.16 -3.6
31,733 - GFF5250 0.16 -2.4
31,770 + GFF5250 0.22 -0.0
31,770 + GFF5250 0.22 -2.3
31,771 - GFF5250 0.23 -2.0
31,921 + GFF5250 0.49 -1.6
31,921 + GFF5250 0.49 -1.1
31,921 + GFF5250 0.49 -1.9
31,921 + GFF5250 0.49 -1.7
31,922 - GFF5250 0.49 -3.5
31,922 - GFF5250 0.49 -1.0
31,922 - GFF5250 0.49 -3.4
31,922 - GFF5250 0.49 -2.7
32,294 - -1.5
32,373 - GFF5251 0.12 -0.3
32,437 + GFF5251 0.17 -2.5
32,438 - GFF5251 0.17 -1.2
32,506 + GFF5251 0.23 -0.6
32,585 - GFF5251 0.29 +0.7
32,594 + GFF5251 0.30 -2.3
32,594 + GFF5251 0.30 -2.0
32,594 + GFF5251 0.30 +0.3
32,594 + GFF5251 0.30 -3.0
32,632 + GFF5251 0.33 -1.7
32,633 - GFF5251 0.33 +1.9
32,633 - GFF5251 0.33 -0.0
32,633 - GFF5251 0.33 -0.6
32,743 + GFF5251 0.42 -3.5
32,980 + GFF5251 0.62 -1.6
32,980 + GFF5251 0.62 -2.4
32,981 - GFF5251 0.62 +0.2
32,983 + GFF5251 0.62 -3.8
32,984 - GFF5251 0.62 -1.4
32,984 - GFF5251 0.62 -2.2
33,183 + GFF5251 0.78 -0.7
33,183 + GFF5251 0.78 -1.6
33,183 + GFF5251 0.78 -1.2
33,183 + GFF5251 0.78 -0.4
33,184 - GFF5251 0.79 -0.3
33,208 + GFF5251 0.81 -2.0
33,209 - GFF5251 0.81 -0.6

Or see this region's nucleotide sequence