Experiment: D-Glucose and D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3 and GFF4 are separated by 69 nucleotides GFF4 and GFF5 are separated by 168 nucleotides GFF5 and GFF6 are separated by 134 nucleotides GFF6 and GFF7 are separated by 33 nucleotides
GFF3 - Anhydro-N-acetylmuramic acid kinase, at 3,144 to 4,235
GFF3
GFF4 - Iron-sulfur cluster insertion protein ErpA, at 4,305 to 4,655
GFF4
GFF5 - N-acetyl-gamma-glutamyl-phosphate reductase, at 4,824 to 5,858
GFF5
GFF6 - hypothetical protein, at 5,993 to 6,421
GFF6
GFF7 - hypothetical protein, at 6,455 to 7,423
GFF7
Position (kb)
4
5
6 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3.973 kb on + strand, within GFF3 at 4.071 kb on - strand, within GFF3 at 4.071 kb on - strand, within GFF3 at 4.117 kb on + strand, within GFF3 at 4.118 kb on - strand, within GFF3 at 4.118 kb on - strand, within GFF3 at 4.211 kb on - strand at 4.234 kb on - strand at 4.245 kb on - strand at 4.686 kb on - strand at 4.735 kb on - strand at 4.794 kb on + strand at 4.794 kb on + strand at 4.794 kb on + strand at 4.794 kb on + strand at 4.794 kb on + strand at 4.794 kb on + strand at 4.795 kb on - strand at 4.795 kb on - strand at 4.795 kb on - strand at 4.826 kb on - strand at 5.066 kb on - strand, within GFF5 at 5.066 kb on - strand, within GFF5 at 5.066 kb on - strand, within GFF5 at 5.066 kb on - strand, within GFF5 at 5.119 kb on + strand, within GFF5 at 5.120 kb on - strand, within GFF5 at 5.249 kb on - strand, within GFF5 at 5.279 kb on - strand, within GFF5 at 5.279 kb on - strand, within GFF5 at 5.289 kb on + strand, within GFF5 at 5.289 kb on + strand, within GFF5 at 5.289 kb on + strand, within GFF5 at 5.289 kb on + strand, within GFF5 at 5.290 kb on - strand, within GFF5 at 5.473 kb on + strand, within GFF5 at 5.474 kb on - strand, within GFF5 at 5.519 kb on - strand, within GFF5 at 5.702 kb on - strand, within GFF5 at 5.722 kb on + strand, within GFF5 at 5.722 kb on + strand, within GFF5 at 5.824 kb on + strand at 5.858 kb on + strand at 5.859 kb on - strand at 5.900 kb on - strand at 5.900 kb on - strand at 5.956 kb on + strand at 5.956 kb on + strand at 5.956 kb on + strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.957 kb on - strand at 5.959 kb on + strand at 5.959 kb on + strand at 5.959 kb on + strand at 5.959 kb on + strand at 5.960 kb on - strand at 5.960 kb on - strand at 5.960 kb on - strand at 5.960 kb on - strand at 5.960 kb on - strand at 5.960 kb on - strand at 5.960 kb on - strand at 5.960 kb on - strand at 6.419 kb on + strand at 6.419 kb on + strand at 6.419 kb on + strand at 6.419 kb on + strand at 6.419 kb on + strand at 6.420 kb on - strand at 6.432 kb on + strand at 6.432 kb on + strand at 6.498 kb on + strand at 6.501 kb on + strand at 6.502 kb on - strand at 6.502 kb on - strand at 6.502 kb on - strand at 6.668 kb on + strand, within GFF7 at 6.754 kb on + strand, within GFF7 at 6.803 kb on + strand, within GFF7 at 6.803 kb on + strand, within GFF7 at 6.804 kb on - strand, within GFF7 at 6.804 kb on - strand, within GFF7 at 6.804 kb on - strand, within GFF7
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose and D,L-Lactate (C) remove 3,973 + GFF3 0.76 -0.5 4,071 - GFF3 0.85 +0.3 4,071 - GFF3 0.85 -0.2 4,117 + GFF3 0.89 +0.3 4,118 - GFF3 0.89 +0.3 4,118 - GFF3 0.89 +1.1 4,211 - +0.3 4,234 - -0.4 4,245 - +0.5 4,686 - +0.3 4,735 - -0.5 4,794 + +0.9 4,794 + -0.1 4,794 + +0.9 4,794 + -0.2 4,794 + -0.1 4,794 + -1.2 4,795 - -0.0 4,795 - -0.5 4,795 - -1.3 4,826 - -0.8 5,066 - GFF5 0.23 -1.3 5,066 - GFF5 0.23 -4.1 5,066 - GFF5 0.23 -2.1 5,066 - GFF5 0.23 -2.5 5,119 + GFF5 0.29 -2.2 5,120 - GFF5 0.29 -3.5 5,249 - GFF5 0.41 -1.9 5,279 - GFF5 0.44 -3.2 5,279 - GFF5 0.44 -1.0 5,289 + GFF5 0.45 -0.8 5,289 + GFF5 0.45 -2.3 5,289 + GFF5 0.45 -2.0 5,289 + GFF5 0.45 -3.7 5,290 - GFF5 0.45 -2.4 5,473 + GFF5 0.63 -1.7 5,474 - GFF5 0.63 -1.1 5,519 - GFF5 0.67 -0.3 5,702 - GFF5 0.85 +0.4 5,722 + GFF5 0.87 -2.7 5,722 + GFF5 0.87 -3.5 5,824 + -2.2 5,858 + -3.7 5,859 - -3.5 5,900 - -0.7 5,900 - +0.4 5,956 + +0.2 5,956 + +0.6 5,956 + +0.3 5,957 - -0.3 5,957 - -0.3 5,957 - +0.4 5,957 - +0.7 5,957 - -1.8 5,957 - -0.2 5,957 - -0.1 5,957 - +0.9 5,959 + -1.0 5,959 + +0.1 5,959 + +0.8 5,959 + +0.0 5,960 - -0.4 5,960 - -0.1 5,960 - -0.3 5,960 - -0.3 5,960 - -0.3 5,960 - +1.3 5,960 - +0.2 5,960 - +0.0 6,419 + -0.4 6,419 + +0.2 6,419 + -0.6 6,419 + +0.3 6,419 + -0.3 6,420 - +0.6 6,432 + +0.0 6,432 + -1.8 6,498 + -0.8 6,501 + +0.6 6,502 - -0.9 6,502 - -0.8 6,502 - +0.2 6,668 + GFF7 0.22 -1.1 6,754 + GFF7 0.31 +0.2 6,803 + GFF7 0.36 -1.0 6,803 + GFF7 0.36 -0.3 6,804 - GFF7 0.36 -0.9 6,804 - GFF7 0.36 +0.8 6,804 - GFF7 0.36 -0.3
Or see this region's nucleotide sequence