Experiment: M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.1X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS00910 and argA are separated by 157 nucleotides argA and argE are separated by 115 nucleotides
COO64_RS00910: COO64_RS00910 - glutamine synthetase family protein, at 194,259 to 195,635
_RS00910
COO64_RS00915: argA - amino-acid N-acetyltransferase, at 195,793 to 197,091
argA
COO64_RS00920: argE - acetylornithine deacetylase, at 197,207 to 198,364
argE
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 194.793 kb on + strand, within COO64_RS00910 at 194.793 kb on + strand, within COO64_RS00910 at 194.794 kb on - strand, within COO64_RS00910 at 194.794 kb on - strand, within COO64_RS00910 at 194.794 kb on - strand, within COO64_RS00910 at 194.817 kb on + strand, within COO64_RS00910 at 194.818 kb on - strand, within COO64_RS00910 at 194.956 kb on - strand, within COO64_RS00910 at 194.994 kb on + strand, within COO64_RS00910 at 195.012 kb on - strand, within COO64_RS00910 at 195.069 kb on - strand, within COO64_RS00910 at 195.069 kb on - strand, within COO64_RS00910 at 195.069 kb on - strand, within COO64_RS00910 at 195.069 kb on - strand, within COO64_RS00910 at 195.069 kb on - strand, within COO64_RS00910 at 195.069 kb on - strand, within COO64_RS00910 at 195.211 kb on - strand, within COO64_RS00910 at 195.234 kb on + strand, within COO64_RS00910 at 195.234 kb on + strand, within COO64_RS00910 at 195.235 kb on - strand, within COO64_RS00910 at 195.235 kb on - strand, within COO64_RS00910 at 195.235 kb on - strand, within COO64_RS00910 at 195.235 kb on - strand, within COO64_RS00910 at 195.235 kb on - strand, within COO64_RS00910 at 195.272 kb on + strand, within COO64_RS00910 at 195.354 kb on + strand, within COO64_RS00910 at 195.441 kb on + strand, within COO64_RS00910 at 195.441 kb on + strand, within COO64_RS00910 at 195.442 kb on - strand, within COO64_RS00910 at 195.522 kb on + strand at 195.522 kb on + strand at 195.522 kb on + strand at 195.522 kb on + strand at 195.523 kb on - strand at 195.523 kb on - strand at 195.523 kb on - strand at 195.523 kb on - strand at 195.523 kb on - strand at 195.523 kb on - strand at 195.523 kb on - strand at 195.537 kb on + strand at 195.537 kb on + strand at 195.537 kb on + strand at 195.537 kb on + strand at 195.537 kb on + strand at 195.538 kb on - strand at 195.545 kb on + strand at 195.545 kb on + strand at 195.545 kb on + strand at 195.546 kb on - strand at 195.561 kb on - strand at 195.609 kb on + strand at 195.609 kb on + strand at 195.609 kb on + strand at 195.609 kb on + strand at 195.610 kb on - strand at 195.610 kb on - strand at 195.610 kb on - strand at 195.610 kb on - strand at 195.610 kb on - strand at 195.618 kb on + strand at 195.626 kb on + strand at 195.626 kb on + strand at 195.633 kb on + strand at 195.634 kb on - strand at 195.736 kb on - strand at 195.736 kb on - strand at 195.736 kb on - strand at 195.736 kb on - strand at 195.736 kb on - strand at 195.736 kb on - strand at 195.822 kb on + strand at 195.823 kb on - strand at 195.830 kb on + strand at 195.830 kb on + strand at 195.830 kb on + strand at 195.830 kb on + strand at 195.830 kb on + strand at 195.831 kb on - strand at 195.831 kb on - strand at 195.836 kb on + strand at 195.836 kb on + strand at 195.837 kb on - strand at 195.837 kb on - strand at 195.837 kb on - strand at 196.064 kb on + strand, within argA at 196.313 kb on + strand, within argA at 196.313 kb on + strand, within argA at 196.313 kb on + strand, within argA at 196.314 kb on - strand, within argA at 196.314 kb on - strand, within argA at 196.314 kb on - strand, within argA at 196.314 kb on - strand, within argA at 196.323 kb on + strand, within argA at 196.324 kb on - strand, within argA at 196.324 kb on - strand, within argA at 196.385 kb on + strand, within argA at 196.667 kb on + strand, within argA at 196.667 kb on + strand, within argA at 196.668 kb on - strand, within argA at 196.668 kb on - strand, within argA at 196.668 kb on - strand, within argA at 196.862 kb on + strand, within argA at 196.863 kb on - strand, within argA at 196.863 kb on - strand, within argA at 196.936 kb on + strand, within argA at 196.936 kb on + strand, within argA at 196.937 kb on - strand, within argA at 196.974 kb on + strand at 197.051 kb on + strand at 197.051 kb on + strand at 197.051 kb on + strand at 197.051 kb on + strand at 197.051 kb on + strand at 197.052 kb on - strand at 197.052 kb on - strand at 197.052 kb on - strand at 197.052 kb on - strand at 197.082 kb on - strand at 197.107 kb on - strand at 197.114 kb on - strand at 197.219 kb on + strand at 197.235 kb on + strand at 197.236 kb on - strand at 197.236 kb on - strand at 197.236 kb on - strand at 197.259 kb on - strand at 197.269 kb on + strand at 197.269 kb on + strand at 197.269 kb on + strand at 197.269 kb on + strand at 197.270 kb on - strand at 197.270 kb on - strand at 197.295 kb on + strand at 197.295 kb on + strand at 197.295 kb on + strand at 197.296 kb on - strand at 197.296 kb on - strand at 197.298 kb on - strand at 197.370 kb on + strand, within argE at 197.371 kb on - strand, within argE at 197.416 kb on + strand, within argE at 197.435 kb on + strand, within argE at 197.493 kb on + strand, within argE at 197.493 kb on + strand, within argE at 197.494 kb on - strand, within argE at 197.620 kb on + strand, within argE at 197.620 kb on + strand, within argE at 197.620 kb on + strand, within argE at 197.621 kb on - strand, within argE at 197.621 kb on - strand, within argE at 197.621 kb on - strand, within argE at 197.621 kb on - strand, within argE at 197.621 kb on - strand, within argE at 197.647 kb on + strand, within argE at 197.647 kb on + strand, within argE at 197.647 kb on + strand, within argE at 197.647 kb on + strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 197.648 kb on - strand, within argE at 198.059 kb on + strand, within argE at 198.059 kb on + strand, within argE at 198.060 kb on - strand, within argE
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant; Pseudomonas DC3000 grown in LB 0.1X remove 194,793 + COO64_RS00910 0.39 +0.3 194,793 + COO64_RS00910 0.39 +0.5 194,794 - COO64_RS00910 0.39 +0.3 194,794 - COO64_RS00910 0.39 -1.1 194,794 - COO64_RS00910 0.39 +0.6 194,817 + COO64_RS00910 0.41 -1.7 194,818 - COO64_RS00910 0.41 -0.4 194,956 - COO64_RS00910 0.51 +0.3 194,994 + COO64_RS00910 0.53 +0.0 195,012 - COO64_RS00910 0.55 -0.4 195,069 - COO64_RS00910 0.59 -0.4 195,069 - COO64_RS00910 0.59 -0.9 195,069 - COO64_RS00910 0.59 -0.5 195,069 - COO64_RS00910 0.59 -0.5 195,069 - COO64_RS00910 0.59 -0.8 195,069 - COO64_RS00910 0.59 +0.6 195,211 - COO64_RS00910 0.69 -3.0 195,234 + COO64_RS00910 0.71 -1.8 195,234 + COO64_RS00910 0.71 -0.3 195,235 - COO64_RS00910 0.71 +1.1 195,235 - COO64_RS00910 0.71 -0.6 195,235 - COO64_RS00910 0.71 -0.5 195,235 - COO64_RS00910 0.71 +0.9 195,235 - COO64_RS00910 0.71 +0.1 195,272 + COO64_RS00910 0.74 -0.7 195,354 + COO64_RS00910 0.80 -0.3 195,441 + COO64_RS00910 0.86 -0.1 195,441 + COO64_RS00910 0.86 -0.8 195,442 - COO64_RS00910 0.86 +0.1 195,522 + -1.0 195,522 + +0.3 195,522 + -0.4 195,522 + -0.1 195,523 - +1.1 195,523 - -0.7 195,523 - +0.2 195,523 - +0.4 195,523 - +0.1 195,523 - -0.4 195,523 - -0.1 195,537 + -0.9 195,537 + -0.8 195,537 + -0.4 195,537 + +2.3 195,537 + -0.2 195,538 - -0.0 195,545 + +0.2 195,545 + +0.6 195,545 + +0.5 195,546 - -0.4 195,561 - -0.4 195,609 + -0.5 195,609 + +1.5 195,609 + +1.1 195,609 + -0.1 195,610 - +1.3 195,610 - +1.0 195,610 - -0.3 195,610 - -1.0 195,610 - -0.1 195,618 + -0.1 195,626 + -1.2 195,626 + -0.3 195,633 + -0.8 195,634 - +0.3 195,736 - -0.6 195,736 - +0.1 195,736 - +0.2 195,736 - -0.9 195,736 - -0.8 195,736 - -1.5 195,822 + +0.9 195,823 - -2.2 195,830 + -1.1 195,830 + -1.2 195,830 + +0.4 195,830 + -0.1 195,830 + -1.5 195,831 - -1.8 195,831 - -1.3 195,836 + +0.2 195,836 + -0.7 195,837 - -0.3 195,837 - -1.2 195,837 - -2.4 196,064 + argA COO64_RS00915 0.21 -1.1 196,313 + argA COO64_RS00915 0.40 -1.8 196,313 + argA COO64_RS00915 0.40 -0.9 196,313 + argA COO64_RS00915 0.40 -0.0 196,314 - argA COO64_RS00915 0.40 -2.5 196,314 - argA COO64_RS00915 0.40 -1.0 196,314 - argA COO64_RS00915 0.40 -0.5 196,314 - argA COO64_RS00915 0.40 -1.5 196,323 + argA COO64_RS00915 0.41 -0.9 196,324 - argA COO64_RS00915 0.41 -0.4 196,324 - argA COO64_RS00915 0.41 -0.7 196,385 + argA COO64_RS00915 0.46 +0.3 196,667 + argA COO64_RS00915 0.67 -0.8 196,667 + argA COO64_RS00915 0.67 +0.5 196,668 - argA COO64_RS00915 0.67 -2.3 196,668 - argA COO64_RS00915 0.67 -0.9 196,668 - argA COO64_RS00915 0.67 +0.3 196,862 + argA COO64_RS00915 0.82 -1.9 196,863 - argA COO64_RS00915 0.82 -2.1 196,863 - argA COO64_RS00915 0.82 -0.4 196,936 + argA COO64_RS00915 0.88 -1.8 196,936 + argA COO64_RS00915 0.88 +0.6 196,937 - argA COO64_RS00915 0.88 +0.9 196,974 + +0.4 197,051 + -0.6 197,051 + -1.6 197,051 + -0.9 197,051 + -0.6 197,051 + -0.8 197,052 - -0.1 197,052 - -2.4 197,052 - -0.9 197,052 - -0.5 197,082 - -2.1 197,107 - -0.6 197,114 - +0.4 197,219 + +0.9 197,235 + +0.8 197,236 - -1.4 197,236 - +0.6 197,236 - -0.3 197,259 - -1.0 197,269 + -0.1 197,269 + -0.2 197,269 + +0.3 197,269 + -0.7 197,270 - -2.5 197,270 - -0.5 197,295 + -1.5 197,295 + -0.4 197,295 + +1.1 197,296 - +0.4 197,296 - -0.4 197,298 - +0.7 197,370 + argE COO64_RS00920 0.14 -1.2 197,371 - argE COO64_RS00920 0.14 -1.4 197,416 + argE COO64_RS00920 0.18 +0.4 197,435 + argE COO64_RS00920 0.20 -0.4 197,493 + argE COO64_RS00920 0.25 +0.8 197,493 + argE COO64_RS00920 0.25 +0.2 197,494 - argE COO64_RS00920 0.25 +0.0 197,620 + argE COO64_RS00920 0.36 +0.2 197,620 + argE COO64_RS00920 0.36 -0.1 197,620 + argE COO64_RS00920 0.36 +0.1 197,621 - argE COO64_RS00920 0.36 -0.1 197,621 - argE COO64_RS00920 0.36 -0.1 197,621 - argE COO64_RS00920 0.36 +0.6 197,621 - argE COO64_RS00920 0.36 +1.0 197,621 - argE COO64_RS00920 0.36 -0.2 197,647 + argE COO64_RS00920 0.38 -0.1 197,647 + argE COO64_RS00920 0.38 -0.1 197,647 + argE COO64_RS00920 0.38 -0.7 197,647 + argE COO64_RS00920 0.38 -0.3 197,648 - argE COO64_RS00920 0.38 -0.7 197,648 - argE COO64_RS00920 0.38 -1.5 197,648 - argE COO64_RS00920 0.38 +0.2 197,648 - argE COO64_RS00920 0.38 +0.4 197,648 - argE COO64_RS00920 0.38 -0.0 197,648 - argE COO64_RS00920 0.38 +0.6 197,648 - argE COO64_RS00920 0.38 -0.7 197,648 - argE COO64_RS00920 0.38 -0.2 197,648 - argE COO64_RS00920 0.38 +0.3 197,648 - argE COO64_RS00920 0.38 -0.1 198,059 + argE COO64_RS00920 0.74 -0.4 198,059 + argE COO64_RS00920 0.74 +1.9 198,060 - argE COO64_RS00920 0.74 -0.1
Or see this region's nucleotide sequence