Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF32

Experiment: Ying_Others16 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF30 and GFF31 overlap by 4 nucleotidesGFF31 and GFF32 are separated by 8 nucleotidesGFF32 and GFF33 overlap by 4 nucleotides GFF30 - Cytosine deaminase (EC 3.5.4.1), at 27,139 to 28,488 GFF30 GFF31 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 28,485 to 29,273 GFF31 GFF32 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 29,282 to 30,058 GFF32 GFF33 - ABC transporter substrate-binding protein, at 30,055 to 31,065 GFF33 Position (kb) 29 30 31Strain fitness (log2 ratio) -1 0 1at 28.558 kb on + strandat 28.747 kb on + strand, within GFF31at 28.805 kb on - strand, within GFF31at 28.993 kb on + strand, within GFF31at 28.994 kb on - strand, within GFF31at 28.994 kb on - strand, within GFF31at 29.353 kb on - strandat 29.463 kb on + strand, within GFF32at 29.463 kb on + strand, within GFF32at 30.155 kb on + strandat 30.156 kb on - strand, within GFF33at 30.326 kb on + strand, within GFF33at 30.332 kb on + strand, within GFF33at 30.333 kb on - strand, within GFF33at 30.428 kb on + strand, within GFF33at 30.429 kb on - strand, within GFF33at 30.570 kb on - strand, within GFF33at 30.570 kb on - strand, within GFF33at 30.893 kb on + strand, within GFF33at 30.893 kb on + strand, within GFF33

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 2
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28,558 + -0.7
28,747 + GFF31 0.33 +0.3
28,805 - GFF31 0.41 +0.7
28,993 + GFF31 0.64 -0.3
28,994 - GFF31 0.65 +0.3
28,994 - GFF31 0.65 +0.2
29,353 - -0.6
29,463 + GFF32 0.23 -0.1
29,463 + GFF32 0.23 +0.7
30,155 + -0.4
30,156 - GFF33 0.10 +0.2
30,326 + GFF33 0.27 +1.5
30,332 + GFF33 0.27 -0.2
30,333 - GFF33 0.27 -0.8
30,428 + GFF33 0.37 -0.2
30,429 - GFF33 0.37 +0.3
30,570 - GFF33 0.51 +0.5
30,570 - GFF33 0.51 -0.7
30,893 + GFF33 0.83 +0.2
30,893 + GFF33 0.83 +0.1

Or see this region's nucleotide sequence