Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2544

Experiment: Ying_Others16 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2542 and GFF2543 overlap by 4 nucleotidesGFF2543 and GFF2544 are separated by 17 nucleotidesGFF2544 and GFF2545 are separated by 10 nucleotidesGFF2545 and GFF2546 overlap by 8 nucleotides GFF2542 - Colanic acid biosysnthesis glycosyl transferase WcaI, at 129,685 to 130,968 GFF2542 GFF2543 - hypothetical protein, at 130,965 to 131,846 GFF2543 GFF2544 - Beta 1,4 glucosyltransferase, at 131,864 to 132,847 GFF2544 GFF2545 - putative NADH dehydrogenase/NAD(P)H nitroreductase, at 132,858 to 133,814 GFF2545 GFF2546 - Colanic acid biosynthesis acetyltransferase WcaF (EC 2.3.1.-), at 133,807 to 134,379 GFF2546 Position (kb) 131 132 133Strain fitness (log2 ratio) -2 -1 0 1at 130.910 kb on + strandat 130.911 kb on - strandat 130.953 kb on - strandat 130.953 kb on - strandat 131.083 kb on + strand, within GFF2543at 131.083 kb on + strand, within GFF2543at 131.084 kb on - strand, within GFF2543at 131.117 kb on - strand, within GFF2543at 131.117 kb on - strand, within GFF2543at 131.117 kb on - strand, within GFF2543at 131.185 kb on + strand, within GFF2543at 131.186 kb on - strand, within GFF2543at 131.186 kb on - strand, within GFF2543at 131.263 kb on + strand, within GFF2543at 131.263 kb on + strand, within GFF2543at 131.264 kb on - strand, within GFF2543at 131.264 kb on - strand, within GFF2543at 131.272 kb on + strand, within GFF2543at 131.355 kb on + strand, within GFF2543at 131.377 kb on + strand, within GFF2543at 131.378 kb on - strand, within GFF2543at 131.587 kb on + strand, within GFF2543at 131.588 kb on - strand, within GFF2543at 131.617 kb on + strand, within GFF2543at 131.629 kb on + strand, within GFF2543at 131.629 kb on + strand, within GFF2543at 131.630 kb on - strand, within GFF2543at 131.633 kb on - strand, within GFF2543at 131.633 kb on - strand, within GFF2543at 131.633 kb on - strand, within GFF2543at 132.076 kb on - strand, within GFF2544at 132.120 kb on + strand, within GFF2544at 132.121 kb on - strand, within GFF2544at 132.135 kb on + strand, within GFF2544at 132.171 kb on + strand, within GFF2544at 132.172 kb on - strand, within GFF2544at 132.297 kb on + strand, within GFF2544at 132.298 kb on - strand, within GFF2544at 132.298 kb on - strand, within GFF2544at 132.321 kb on + strand, within GFF2544at 132.820 kb on - strandat 133.102 kb on + strand, within GFF2545at 133.102 kb on + strand, within GFF2545at 133.103 kb on - strand, within GFF2545at 133.103 kb on - strand, within GFF2545at 133.240 kb on + strand, within GFF2545at 133.241 kb on - strand, within GFF2545at 133.241 kb on - strand, within GFF2545at 133.528 kb on + strand, within GFF2545at 133.529 kb on - strand, within GFF2545at 133.842 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 2
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130,910 + -0.5
130,911 - -0.1
130,953 - -0.3
130,953 - -0.0
131,083 + GFF2543 0.13 -0.0
131,083 + GFF2543 0.13 +0.2
131,084 - GFF2543 0.13 +0.4
131,117 - GFF2543 0.17 +0.2
131,117 - GFF2543 0.17 -0.5
131,117 - GFF2543 0.17 +0.4
131,185 + GFF2543 0.25 +0.7
131,186 - GFF2543 0.25 -0.7
131,186 - GFF2543 0.25 +0.4
131,263 + GFF2543 0.34 +0.1
131,263 + GFF2543 0.34 +0.3
131,264 - GFF2543 0.34 +0.2
131,264 - GFF2543 0.34 -1.0
131,272 + GFF2543 0.35 -0.3
131,355 + GFF2543 0.44 -0.7
131,377 + GFF2543 0.47 -0.4
131,378 - GFF2543 0.47 -0.2
131,587 + GFF2543 0.71 -0.3
131,588 - GFF2543 0.71 +0.6
131,617 + GFF2543 0.74 -0.1
131,629 + GFF2543 0.75 +0.4
131,629 + GFF2543 0.75 +0.1
131,630 - GFF2543 0.75 +0.4
131,633 - GFF2543 0.76 +0.1
131,633 - GFF2543 0.76 +0.3
131,633 - GFF2543 0.76 -0.2
132,076 - GFF2544 0.22 +0.6
132,120 + GFF2544 0.26 +0.1
132,121 - GFF2544 0.26 +0.3
132,135 + GFF2544 0.28 -0.3
132,171 + GFF2544 0.31 -0.2
132,172 - GFF2544 0.31 -0.6
132,297 + GFF2544 0.44 +0.2
132,298 - GFF2544 0.44 +0.0
132,298 - GFF2544 0.44 -0.4
132,321 + GFF2544 0.46 -0.8
132,820 - -0.0
133,102 + GFF2545 0.25 -0.3
133,102 + GFF2545 0.25 -0.5
133,103 - GFF2545 0.26 -0.2
133,103 - GFF2545 0.26 -0.5
133,240 + GFF2545 0.40 +0.1
133,241 - GFF2545 0.40 -0.0
133,241 - GFF2545 0.40 +0.9
133,528 + GFF2545 0.70 -0.6
133,529 - GFF2545 0.70 -0.4
133,842 - -2.1

Or see this region's nucleotide sequence