Experiment: Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaA and COO64_RS17750 are separated by 77 nucleotides COO64_RS17750 and thiC are separated by 403 nucleotides
COO64_RS17745: waaA - lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase, at 67,054 to 68,322
waaA
COO64_RS17750: COO64_RS17750 - TolC family outer membrane protein, at 68,400 to 69,833
_RS17750
COO64_RS17755: thiC - phosphomethylpyrimidine synthase ThiC, at 70,237 to 72,123
thiC
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-2
-1
0
1 at 68.320 kb on + strand at 68.473 kb on + strand at 68.473 kb on + strand at 68.473 kb on + strand at 68.474 kb on - strand at 68.488 kb on + strand at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.563 kb on + strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.564 kb on - strand, within COO64_RS17750 at 68.569 kb on + strand, within COO64_RS17750 at 68.577 kb on - strand, within COO64_RS17750 at 68.577 kb on - strand, within COO64_RS17750 at 68.577 kb on - strand, within COO64_RS17750 at 68.584 kb on + strand, within COO64_RS17750 at 68.584 kb on + strand, within COO64_RS17750 at 68.584 kb on + strand, within COO64_RS17750 at 68.585 kb on - strand, within COO64_RS17750 at 68.602 kb on + strand, within COO64_RS17750 at 68.602 kb on + strand, within COO64_RS17750 at 68.602 kb on + strand, within COO64_RS17750 at 68.602 kb on + strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.603 kb on - strand, within COO64_RS17750 at 68.824 kb on + strand, within COO64_RS17750 at 68.824 kb on + strand, within COO64_RS17750 at 68.825 kb on - strand, within COO64_RS17750 at 68.825 kb on - strand, within COO64_RS17750 at 68.917 kb on + strand, within COO64_RS17750 at 68.917 kb on + strand, within COO64_RS17750 at 68.917 kb on + strand, within COO64_RS17750 at 68.918 kb on - strand, within COO64_RS17750 at 68.918 kb on - strand, within COO64_RS17750 at 68.918 kb on - strand, within COO64_RS17750 at 68.918 kb on - strand, within COO64_RS17750 at 69.055 kb on + strand, within COO64_RS17750 at 69.055 kb on + strand, within COO64_RS17750 at 69.055 kb on + strand, within COO64_RS17750 at 69.056 kb on - strand, within COO64_RS17750 at 69.056 kb on - strand, within COO64_RS17750 at 69.056 kb on - strand, within COO64_RS17750 at 69.056 kb on - strand, within COO64_RS17750 at 69.135 kb on + strand, within COO64_RS17750 at 69.136 kb on - strand, within COO64_RS17750 at 69.136 kb on - strand, within COO64_RS17750 at 69.136 kb on - strand, within COO64_RS17750 at 69.172 kb on + strand, within COO64_RS17750 at 69.172 kb on + strand, within COO64_RS17750 at 69.172 kb on + strand, within COO64_RS17750 at 69.172 kb on + strand, within COO64_RS17750 at 69.172 kb on + strand, within COO64_RS17750 at 69.259 kb on + strand, within COO64_RS17750 at 69.259 kb on + strand, within COO64_RS17750 at 69.259 kb on + strand, within COO64_RS17750 at 69.260 kb on - strand, within COO64_RS17750 at 69.403 kb on + strand, within COO64_RS17750 at 69.404 kb on - strand, within COO64_RS17750 at 69.518 kb on + strand, within COO64_RS17750 at 69.518 kb on + strand, within COO64_RS17750 at 69.518 kb on + strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.519 kb on - strand, within COO64_RS17750 at 69.676 kb on + strand, within COO64_RS17750 at 69.677 kb on - strand, within COO64_RS17750 at 69.708 kb on - strand at 69.727 kb on + strand at 69.727 kb on + strand at 69.727 kb on + strand at 69.728 kb on - strand at 69.791 kb on + strand at 69.791 kb on + strand at 69.792 kb on - strand at 69.833 kb on + strand at 69.834 kb on - strand at 69.876 kb on + strand at 69.876 kb on + strand at 69.877 kb on - strand at 69.877 kb on - strand at 69.877 kb on - strand at 69.877 kb on - strand at 69.877 kb on - strand at 69.906 kb on + strand at 69.907 kb on - strand at 69.907 kb on - strand at 69.919 kb on + strand at 69.920 kb on - strand at 69.920 kb on - strand at 69.920 kb on - strand at 69.920 kb on - strand at 69.920 kb on - strand at 69.965 kb on + strand at 69.965 kb on + strand at 69.966 kb on - strand at 70.155 kb on + strand at 70.155 kb on + strand at 70.156 kb on - strand at 70.156 kb on - strand at 70.212 kb on + strand at 70.212 kb on + strand at 70.212 kb on + strand at 70.213 kb on - strand at 70.213 kb on - strand at 70.213 kb on - strand at 70.242 kb on + strand at 70.243 kb on - strand at 70.243 kb on - strand at 70.243 kb on - strand at 70.257 kb on + strand at 70.258 kb on - strand at 70.258 kb on - strand at 70.258 kb on - strand at 70.298 kb on + strand at 70.299 kb on - strand at 70.327 kb on + strand at 70.327 kb on + strand at 70.432 kb on + strand, within thiC at 70.432 kb on + strand, within thiC at 70.433 kb on - strand, within thiC at 70.433 kb on - strand, within thiC at 70.450 kb on + strand, within thiC at 70.666 kb on + strand, within thiC at 70.667 kb on - strand, within thiC at 70.667 kb on - strand, within thiC at 70.702 kb on + strand, within thiC at 70.702 kb on + strand, within thiC at 70.703 kb on - strand, within thiC
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Pseudomonas DC3000 grown in LB; 0.5 X remove 68,320 + +0.4 68,473 + -1.5 68,473 + +0.7 68,473 + -0.3 68,474 - -0.0 68,488 + -1.2 68,563 + COO64_RS17750 0.11 +0.8 68,563 + COO64_RS17750 0.11 -0.7 68,563 + COO64_RS17750 0.11 -0.7 68,563 + COO64_RS17750 0.11 +0.7 68,563 + COO64_RS17750 0.11 -1.2 68,563 + COO64_RS17750 0.11 +0.2 68,563 + COO64_RS17750 0.11 +0.8 68,563 + COO64_RS17750 0.11 +0.9 68,563 + COO64_RS17750 0.11 -0.3 68,564 - COO64_RS17750 0.11 +0.7 68,564 - COO64_RS17750 0.11 -0.0 68,564 - COO64_RS17750 0.11 -0.0 68,564 - COO64_RS17750 0.11 -0.4 68,564 - COO64_RS17750 0.11 +0.1 68,564 - COO64_RS17750 0.11 +0.8 68,564 - COO64_RS17750 0.11 -0.7 68,564 - COO64_RS17750 0.11 -0.6 68,564 - COO64_RS17750 0.11 -1.1 68,569 + COO64_RS17750 0.12 +0.4 68,577 - COO64_RS17750 0.12 +1.0 68,577 - COO64_RS17750 0.12 -0.3 68,577 - COO64_RS17750 0.12 -0.1 68,584 + COO64_RS17750 0.13 +0.7 68,584 + COO64_RS17750 0.13 -2.0 68,584 + COO64_RS17750 0.13 -0.0 68,585 - COO64_RS17750 0.13 -1.1 68,602 + COO64_RS17750 0.14 -0.6 68,602 + COO64_RS17750 0.14 -1.0 68,602 + COO64_RS17750 0.14 -0.0 68,602 + COO64_RS17750 0.14 -1.0 68,603 - COO64_RS17750 0.14 -0.1 68,603 - COO64_RS17750 0.14 -0.6 68,603 - COO64_RS17750 0.14 +0.7 68,603 - COO64_RS17750 0.14 -0.6 68,603 - COO64_RS17750 0.14 +0.1 68,603 - COO64_RS17750 0.14 -0.1 68,603 - COO64_RS17750 0.14 -1.1 68,824 + COO64_RS17750 0.30 -0.7 68,824 + COO64_RS17750 0.30 -2.3 68,825 - COO64_RS17750 0.30 -1.0 68,825 - COO64_RS17750 0.30 -1.3 68,917 + COO64_RS17750 0.36 -0.6 68,917 + COO64_RS17750 0.36 -0.2 68,917 + COO64_RS17750 0.36 -0.3 68,918 - COO64_RS17750 0.36 -0.3 68,918 - COO64_RS17750 0.36 +0.3 68,918 - COO64_RS17750 0.36 +0.2 68,918 - COO64_RS17750 0.36 -1.1 69,055 + COO64_RS17750 0.46 +0.3 69,055 + COO64_RS17750 0.46 +0.1 69,055 + COO64_RS17750 0.46 +0.8 69,056 - COO64_RS17750 0.46 -0.8 69,056 - COO64_RS17750 0.46 +0.2 69,056 - COO64_RS17750 0.46 -0.6 69,056 - COO64_RS17750 0.46 +0.9 69,135 + COO64_RS17750 0.51 -0.0 69,136 - COO64_RS17750 0.51 +0.3 69,136 - COO64_RS17750 0.51 -0.2 69,136 - COO64_RS17750 0.51 +0.2 69,172 + COO64_RS17750 0.54 +0.0 69,172 + COO64_RS17750 0.54 +0.2 69,172 + COO64_RS17750 0.54 +0.2 69,172 + COO64_RS17750 0.54 +0.1 69,172 + COO64_RS17750 0.54 -0.5 69,259 + COO64_RS17750 0.60 -0.2 69,259 + COO64_RS17750 0.60 -0.2 69,259 + COO64_RS17750 0.60 +0.2 69,260 - COO64_RS17750 0.60 +1.2 69,403 + COO64_RS17750 0.70 +0.4 69,404 - COO64_RS17750 0.70 -0.5 69,518 + COO64_RS17750 0.78 +0.5 69,518 + COO64_RS17750 0.78 +0.2 69,518 + COO64_RS17750 0.78 -0.1 69,519 - COO64_RS17750 0.78 -0.7 69,519 - COO64_RS17750 0.78 +0.8 69,519 - COO64_RS17750 0.78 -0.4 69,519 - COO64_RS17750 0.78 +1.0 69,519 - COO64_RS17750 0.78 +0.2 69,519 - COO64_RS17750 0.78 -0.0 69,519 - COO64_RS17750 0.78 -1.0 69,676 + COO64_RS17750 0.89 -0.4 69,677 - COO64_RS17750 0.89 -0.7 69,708 - -1.2 69,727 + +0.8 69,727 + +0.7 69,727 + -0.3 69,728 - +0.4 69,791 + -2.7 69,791 + -1.0 69,792 - -0.3 69,833 + -0.6 69,834 - -0.7 69,876 + -0.4 69,876 + +0.6 69,877 - -0.6 69,877 - +0.2 69,877 - -1.5 69,877 - -0.1 69,877 - -0.3 69,906 + +1.2 69,907 - +1.2 69,907 - -0.3 69,919 + +0.4 69,920 - -0.1 69,920 - +0.4 69,920 - +0.7 69,920 - -0.2 69,920 - +1.1 69,965 + -0.9 69,965 + +0.3 69,966 - +0.2 70,155 + +0.2 70,155 + -0.3 70,156 - -0.5 70,156 - +0.3 70,212 + +0.4 70,212 + +1.3 70,212 + -0.7 70,213 - -0.2 70,213 - +1.5 70,213 - -0.0 70,242 + +0.8 70,243 - +0.1 70,243 - +0.3 70,243 - -0.3 70,257 + -0.3 70,258 - -0.3 70,258 - -0.1 70,258 - +0.4 70,298 + +0.4 70,299 - +0.8 70,327 + -0.2 70,327 + +0.5 70,432 + thiC COO64_RS17755 0.10 -0.4 70,432 + thiC COO64_RS17755 0.10 -1.3 70,433 - thiC COO64_RS17755 0.10 -1.0 70,433 - thiC COO64_RS17755 0.10 -0.1 70,450 + thiC COO64_RS17755 0.11 -0.0 70,666 + thiC COO64_RS17755 0.23 +0.2 70,667 - thiC COO64_RS17755 0.23 -0.6 70,667 - thiC COO64_RS17755 0.23 -0.3 70,702 + thiC COO64_RS17755 0.25 +0.2 70,702 + thiC COO64_RS17755 0.25 +1.4 70,703 - thiC COO64_RS17755 0.25 -0.3
Or see this region's nucleotide sequence