Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5083 and GFF5084 are separated by 50 nucleotides GFF5084 and GFF5085 are separated by 16 nucleotides GFF5085 and GFF5086 are separated by 3 nucleotides GFF5086 and GFF5087 are separated by 169 nucleotides
GFF5083 - Serine acetyltransferase (EC 2.3.1.30), at 2,097,881 to 2,098,801
GFF5083
GFF5084 - Major membrane protein I, at 2,098,852 to 2,099,781
GFF5084
GFF5085 - Cysteine desulfurase (EC 2.8.1.7), at 2,099,798 to 2,101,360
GFF5085
GFF5086 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 2,101,364 to 2,102,128
GFF5086
GFF5087 - Tricarboxylate transport protein TctC, at 2,102,298 to 2,103,260
GFF5087
Position (kb)
2099
2100
2101
2102 Strain fitness (log2 ratio)
-1
0
1 at 2099.083 kb on + strand, within GFF5084 at 2099.083 kb on + strand, within GFF5084 at 2099.084 kb on - strand, within GFF5084 at 2099.113 kb on + strand, within GFF5084 at 2099.687 kb on - strand, within GFF5084 at 2099.773 kb on + strand at 2099.773 kb on + strand at 2099.773 kb on + strand at 2099.774 kb on - strand at 2100.060 kb on - strand, within GFF5085 at 2100.599 kb on + strand, within GFF5085 at 2101.004 kb on + strand, within GFF5085 at 2101.004 kb on + strand, within GFF5085 at 2101.004 kb on + strand, within GFF5085 at 2101.005 kb on - strand, within GFF5085 at 2101.155 kb on - strand, within GFF5085 at 2101.283 kb on + strand at 2101.283 kb on + strand at 2101.455 kb on + strand, within GFF5086 at 2101.573 kb on - strand, within GFF5086 at 2101.573 kb on - strand, within GFF5086 at 2101.573 kb on - strand, within GFF5086 at 2101.591 kb on - strand, within GFF5086 at 2101.627 kb on - strand, within GFF5086 at 2102.106 kb on + strand at 2102.106 kb on + strand at 2102.106 kb on + strand at 2102.200 kb on + strand at 2102.219 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 2,099,083 + GFF5084 0.25 -1.3 2,099,083 + GFF5084 0.25 -0.0 2,099,084 - GFF5084 0.25 -0.1 2,099,113 + GFF5084 0.28 +0.0 2,099,687 - GFF5084 0.90 +0.5 2,099,773 + +0.3 2,099,773 + +1.2 2,099,773 + +0.3 2,099,774 - -0.0 2,100,060 - GFF5085 0.17 -0.3 2,100,599 + GFF5085 0.51 +0.2 2,101,004 + GFF5085 0.77 -0.9 2,101,004 + GFF5085 0.77 +0.1 2,101,004 + GFF5085 0.77 +0.6 2,101,005 - GFF5085 0.77 -1.0 2,101,155 - GFF5085 0.87 -0.4 2,101,283 + +0.5 2,101,283 + -1.3 2,101,455 + GFF5086 0.12 +1.5 2,101,573 - GFF5086 0.27 -0.1 2,101,573 - GFF5086 0.27 -0.1 2,101,573 - GFF5086 0.27 +0.2 2,101,591 - GFF5086 0.30 +0.8 2,101,627 - GFF5086 0.34 +0.6 2,102,106 + -0.3 2,102,106 + -0.4 2,102,106 + +0.3 2,102,200 + +0.4 2,102,219 + -0.9
Or see this region's nucleotide sequence