Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5085

Experiment: Ying_Nucleobase14 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5083 and GFF5084 are separated by 50 nucleotidesGFF5084 and GFF5085 are separated by 16 nucleotidesGFF5085 and GFF5086 are separated by 3 nucleotidesGFF5086 and GFF5087 are separated by 169 nucleotides GFF5083 - Serine acetyltransferase (EC 2.3.1.30), at 2,097,881 to 2,098,801 GFF5083 GFF5084 - Major membrane protein I, at 2,098,852 to 2,099,781 GFF5084 GFF5085 - Cysteine desulfurase (EC 2.8.1.7), at 2,099,798 to 2,101,360 GFF5085 GFF5086 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 2,101,364 to 2,102,128 GFF5086 GFF5087 - Tricarboxylate transport protein TctC, at 2,102,298 to 2,103,260 GFF5087 Position (kb) 2099 2100 2101 2102Strain fitness (log2 ratio) -1 0 1at 2099.083 kb on + strand, within GFF5084at 2099.083 kb on + strand, within GFF5084at 2099.084 kb on - strand, within GFF5084at 2099.113 kb on + strand, within GFF5084at 2099.687 kb on - strand, within GFF5084at 2099.773 kb on + strandat 2099.773 kb on + strandat 2099.773 kb on + strandat 2099.774 kb on - strandat 2100.060 kb on - strand, within GFF5085at 2100.599 kb on + strand, within GFF5085at 2101.004 kb on + strand, within GFF5085at 2101.004 kb on + strand, within GFF5085at 2101.004 kb on + strand, within GFF5085at 2101.005 kb on - strand, within GFF5085at 2101.155 kb on - strand, within GFF5085at 2101.283 kb on + strandat 2101.283 kb on + strandat 2101.455 kb on + strand, within GFF5086at 2101.573 kb on - strand, within GFF5086at 2101.573 kb on - strand, within GFF5086at 2101.573 kb on - strand, within GFF5086at 2101.591 kb on - strand, within GFF5086at 2101.627 kb on - strand, within GFF5086at 2102.106 kb on + strandat 2102.106 kb on + strandat 2102.106 kb on + strandat 2102.200 kb on + strandat 2102.219 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2
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2,099,083 + GFF5084 0.25 -1.3
2,099,083 + GFF5084 0.25 -0.0
2,099,084 - GFF5084 0.25 -0.1
2,099,113 + GFF5084 0.28 +0.0
2,099,687 - GFF5084 0.90 +0.5
2,099,773 + +0.3
2,099,773 + +1.2
2,099,773 + +0.3
2,099,774 - -0.0
2,100,060 - GFF5085 0.17 -0.3
2,100,599 + GFF5085 0.51 +0.2
2,101,004 + GFF5085 0.77 -0.9
2,101,004 + GFF5085 0.77 +0.1
2,101,004 + GFF5085 0.77 +0.6
2,101,005 - GFF5085 0.77 -1.0
2,101,155 - GFF5085 0.87 -0.4
2,101,283 + +0.5
2,101,283 + -1.3
2,101,455 + GFF5086 0.12 +1.5
2,101,573 - GFF5086 0.27 -0.1
2,101,573 - GFF5086 0.27 -0.1
2,101,573 - GFF5086 0.27 +0.2
2,101,591 - GFF5086 0.30 +0.8
2,101,627 - GFF5086 0.34 +0.6
2,102,106 + -0.3
2,102,106 + -0.4
2,102,106 + +0.3
2,102,200 + +0.4
2,102,219 + -0.9

Or see this region's nucleotide sequence