Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4550 and GFF4551 are separated by 4 nucleotides GFF4551 and GFF4552 are separated by 61 nucleotides GFF4552 and GFF4553 are separated by 51 nucleotides
GFF4550 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 1,481,776 to 1,482,948
GFF4550
GFF4551 - Twitching motility protein PilT, at 1,482,953 to 1,484,098
GFF4551
GFF4552 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 1,484,160 to 1,484,984
GFF4552
GFF4553 - N-succinyl-L,L-diaminopimelate aminotransferase alternative (EC 2.6.1.17), at 1,485,036 to 1,486,259
GFF4553
Position (kb)
1482
1483
1484
1485 Strain fitness (log2 ratio)
-1
0
1 at 1483.374 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.404 kb on + strand, within GFF4551 at 1483.405 kb on - strand, within GFF4551 at 1483.455 kb on + strand, within GFF4551 at 1483.608 kb on + strand, within GFF4551 at 1483.615 kb on - strand, within GFF4551 at 1483.615 kb on - strand, within GFF4551 at 1483.812 kb on + strand, within GFF4551 at 1483.813 kb on - strand, within GFF4551 at 1483.813 kb on - strand, within GFF4551 at 1484.047 kb on + strand at 1484.150 kb on + strand at 1484.150 kb on + strand at 1484.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 1,483,374 + GFF4551 0.37 +0.1 1,483,404 + GFF4551 0.39 +1.0 1,483,404 + GFF4551 0.39 +0.4 1,483,404 + GFF4551 0.39 +0.1 1,483,404 + GFF4551 0.39 +1.1 1,483,404 + GFF4551 0.39 +0.5 1,483,404 + GFF4551 0.39 -0.1 1,483,404 + GFF4551 0.39 -1.0 1,483,405 - GFF4551 0.39 -1.5 1,483,455 + GFF4551 0.44 +0.2 1,483,608 + GFF4551 0.57 -1.0 1,483,615 - GFF4551 0.58 +0.3 1,483,615 - GFF4551 0.58 -0.1 1,483,812 + GFF4551 0.75 +0.5 1,483,813 - GFF4551 0.75 +0.2 1,483,813 - GFF4551 0.75 -0.0 1,484,047 + -0.6 1,484,150 + +0.2 1,484,150 + -0.2 1,484,151 - -0.1
Or see this region's nucleotide sequence