Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3472 and GFF3473 are separated by 30 nucleotides GFF3473 and GFF3474 are separated by 43 nucleotides GFF3474 and GFF3475 are separated by 16 nucleotides GFF3475 and GFF3476 overlap by 4 nucleotides
GFF3472 - Enoyl-CoA hydratase (EC 4.2.1.17), at 353,799 to 354,599
GFF3472
GFF3473 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 354,630 to 355,412
GFF3473
GFF3474 - hypothetical protein, at 355,456 to 356,646
GFF3474
GFF3475 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 356,663 to 357,430
GFF3475
GFF3476 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 357,427 to 358,143
GFF3476
Position (kb) 355
356
357 Strain fitness (log2 ratio) -1
0
1 at 354.615 kb on + strand at 354.616 kb on - strand at 354.765 kb on - strand, within GFF3473 at 354.765 kb on - strand, within GFF3473 at 354.789 kb on + strand, within GFF3473 at 354.989 kb on + strand, within GFF3473 at 355.028 kb on + strand, within GFF3473 at 355.125 kb on + strand, within GFF3473 at 355.347 kb on - strand at 355.416 kb on - strand at 355.423 kb on + strand at 355.610 kb on - strand, within GFF3474 at 355.691 kb on + strand, within GFF3474 at 355.691 kb on + strand, within GFF3474 at 355.711 kb on + strand, within GFF3474 at 355.787 kb on + strand, within GFF3474 at 355.787 kb on + strand, within GFF3474 at 355.838 kb on + strand, within GFF3474 at 355.855 kb on - strand, within GFF3474 at 355.855 kb on - strand, within GFF3474 at 356.230 kb on + strand, within GFF3474 at 356.230 kb on + strand, within GFF3474 at 356.389 kb on + strand, within GFF3474 at 356.436 kb on + strand, within GFF3474 at 356.619 kb on + strand at 356.742 kb on + strand, within GFF3475 at 356.743 kb on - strand, within GFF3475 at 356.917 kb on + strand, within GFF3475
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 354,615 + +0.3 354,616 - +0.1 354,765 - GFF3473 0.17 -0.1 354,765 - GFF3473 0.17 +0.8 354,789 + GFF3473 0.20 +1.2 354,989 + GFF3473 0.46 +0.2 355,028 + GFF3473 0.51 +0.4 355,125 + GFF3473 0.63 +0.6 355,347 - -0.4 355,416 - -0.9 355,423 + -0.3 355,610 - GFF3474 0.13 +0.5 355,691 + GFF3474 0.20 +0.2 355,691 + GFF3474 0.20 -0.6 355,711 + GFF3474 0.21 +1.1 355,787 + GFF3474 0.28 -0.7 355,787 + GFF3474 0.28 +0.0 355,838 + GFF3474 0.32 -0.5 355,855 - GFF3474 0.34 -0.4 355,855 - GFF3474 0.34 -0.1 356,230 + GFF3474 0.65 +0.1 356,230 + GFF3474 0.65 -1.5 356,389 + GFF3474 0.78 -0.3 356,436 + GFF3474 0.82 -0.5 356,619 + -0.0 356,742 + GFF3475 0.10 -0.1 356,743 - GFF3475 0.10 -0.3 356,917 + GFF3475 0.33 -0.8
Or see this region's nucleotide sequence