Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3360 and GFF3361 are separated by 30 nucleotides GFF3361 and GFF3362 are separated by 15 nucleotides GFF3362 and GFF3363 are separated by 40 nucleotides GFF3363 and GFF3364 are separated by 29 nucleotides
GFF3360 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 245,802 to 246,890
GFF3360
GFF3361 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 246,921 to 247,571
GFF3361
GFF3362 - hypothetical protein, at 247,587 to 247,748
GFF3362
GFF3363 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 247,789 to 249,210
GFF3363
GFF3364 - Transcriptional regulator, at 249,240 to 250,166
GFF3364
Position (kb) 247
248
249
250 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 246.997 kb on + strand, within GFF3361 at 246.997 kb on + strand, within GFF3361 at 247.042 kb on + strand, within GFF3361 at 247.042 kb on + strand, within GFF3361 at 247.042 kb on + strand, within GFF3361 at 247.043 kb on - strand, within GFF3361 at 247.043 kb on - strand, within GFF3361 at 247.043 kb on - strand, within GFF3361 at 247.043 kb on - strand, within GFF3361 at 247.237 kb on + strand, within GFF3361 at 247.237 kb on + strand, within GFF3361 at 247.237 kb on + strand, within GFF3361 at 247.268 kb on - strand, within GFF3361 at 247.351 kb on + strand, within GFF3361 at 247.430 kb on - strand, within GFF3361 at 247.588 kb on + strand at 247.588 kb on + strand at 247.588 kb on + strand at 247.588 kb on + strand at 247.589 kb on - strand at 247.589 kb on - strand at 247.589 kb on - strand at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.225 kb on + strand, within GFF3363 at 248.226 kb on - strand, within GFF3363 at 248.226 kb on - strand, within GFF3363 at 248.226 kb on - strand, within GFF3363 at 248.226 kb on - strand, within GFF3363 at 248.226 kb on - strand, within GFF3363 at 248.226 kb on - strand, within GFF3363 at 248.417 kb on + strand, within GFF3363 at 248.474 kb on + strand, within GFF3363 at 248.475 kb on - strand, within GFF3363 at 248.603 kb on + strand, within GFF3363 at 248.603 kb on + strand, within GFF3363 at 248.603 kb on + strand, within GFF3363 at 248.805 kb on - strand, within GFF3363 at 249.089 kb on + strand at 249.089 kb on + strand at 249.089 kb on + strand at 249.090 kb on - strand at 249.090 kb on - strand at 249.090 kb on - strand at 249.090 kb on - strand at 249.090 kb on - strand at 249.090 kb on - strand at 249.134 kb on + strand at 249.134 kb on + strand at 249.135 kb on - strand at 249.162 kb on + strand at 249.162 kb on + strand at 249.163 kb on - strand at 249.194 kb on + strand at 249.194 kb on + strand at 249.194 kb on + strand at 249.195 kb on - strand at 249.195 kb on - strand at 249.195 kb on - strand at 249.195 kb on - strand at 249.884 kb on - strand, within GFF3364 at 249.884 kb on - strand, within GFF3364
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 246,997 + GFF3361 0.12 -2.1 246,997 + GFF3361 0.12 -3.7 247,042 + GFF3361 0.19 -4.0 247,042 + GFF3361 0.19 -5.1 247,042 + GFF3361 0.19 -4.1 247,043 - GFF3361 0.19 -2.5 247,043 - GFF3361 0.19 -2.4 247,043 - GFF3361 0.19 -1.7 247,043 - GFF3361 0.19 -0.9 247,237 + GFF3361 0.49 -4.5 247,237 + GFF3361 0.49 -3.7 247,237 + GFF3361 0.49 -2.4 247,268 - GFF3361 0.53 -1.8 247,351 + GFF3361 0.66 -3.2 247,430 - GFF3361 0.78 -2.3 247,588 + -1.7 247,588 + -0.1 247,588 + -0.2 247,588 + -0.9 247,589 - -0.1 247,589 - +0.3 247,589 - +0.4 248,225 + GFF3363 0.31 -2.5 248,225 + GFF3363 0.31 -1.6 248,225 + GFF3363 0.31 -1.2 248,225 + GFF3363 0.31 -3.9 248,225 + GFF3363 0.31 -3.6 248,225 + GFF3363 0.31 -4.3 248,225 + GFF3363 0.31 -1.7 248,225 + GFF3363 0.31 -1.7 248,226 - GFF3363 0.31 -1.1 248,226 - GFF3363 0.31 -1.5 248,226 - GFF3363 0.31 -2.6 248,226 - GFF3363 0.31 -2.1 248,226 - GFF3363 0.31 -4.0 248,226 - GFF3363 0.31 -2.5 248,417 + GFF3363 0.44 -2.7 248,474 + GFF3363 0.48 -1.5 248,475 - GFF3363 0.48 -0.2 248,603 + GFF3363 0.57 -3.3 248,603 + GFF3363 0.57 -3.0 248,603 + GFF3363 0.57 -2.9 248,805 - GFF3363 0.71 -1.5 249,089 + -1.8 249,089 + -2.5 249,089 + -3.0 249,090 - -1.5 249,090 - -4.0 249,090 - -4.2 249,090 - -2.8 249,090 - -2.7 249,090 - -1.7 249,134 + -3.4 249,134 + -1.7 249,135 - -0.8 249,162 + -2.1 249,162 + -1.1 249,163 - -2.7 249,194 + -4.2 249,194 + -1.8 249,194 + -4.1 249,195 - -2.5 249,195 - -1.7 249,195 - -1.8 249,195 - -3.5 249,884 - GFF3364 0.69 +0.0 249,884 - GFF3364 0.69 -1.0
Or see this region's nucleotide sequence