Experiment: Ying_Nucleobase14 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3309 and GFF3310 are separated by 95 nucleotides GFF3310 and GFF3311 overlap by 31 nucleotides GFF3311 and GFF3312 are separated by 6 nucleotides
GFF3309 - Uricase (urate oxidase) (EC 1.7.3.3), at 196,352 to 197,206
GFF3309
GFF3310 - Transcriptional regulator, GntR family, at 197,302 to 198,027
GFF3310
GFF3311 - 5-Hydroxyisourate Hydrolase (HIUase) (EC 3.5.2.17), at 197,997 to 198,395
GFF3311
GFF3312 - Protocatechuate 4,5-dioxygenase beta chain (EC 1.13.11.8), at 198,402 to 199,688
GFF3312
Position (kb) 197
198
199 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 197.065 kb on - strand, within GFF3309 at 197.070 kb on + strand, within GFF3309 at 197.071 kb on - strand, within GFF3309 at 197.295 kb on + strand at 197.296 kb on - strand at 197.483 kb on + strand, within GFF3310 at 197.484 kb on - strand, within GFF3310 at 198.222 kb on + strand, within GFF3311 at 198.223 kb on - strand, within GFF3311 at 198.249 kb on + strand, within GFF3311 at 198.249 kb on + strand, within GFF3311 at 198.373 kb on - strand at 198.518 kb on - strand at 198.767 kb on - strand, within GFF3312 at 198.767 kb on - strand, within GFF3312 at 198.866 kb on - strand, within GFF3312 at 198.904 kb on + strand, within GFF3312 at 198.904 kb on + strand, within GFF3312 at 198.905 kb on - strand, within GFF3312 at 199.160 kb on - strand, within GFF3312 at 199.199 kb on - strand, within GFF3312
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2 remove 197,065 - GFF3309 0.83 -4.0 197,070 + GFF3309 0.84 -3.3 197,071 - GFF3309 0.84 -1.0 197,295 + -0.7 197,296 - -2.3 197,483 + GFF3310 0.25 -1.3 197,484 - GFF3310 0.25 +0.2 198,222 + GFF3311 0.56 -4.1 198,223 - GFF3311 0.57 -3.8 198,249 + GFF3311 0.63 -2.2 198,249 + GFF3311 0.63 -2.3 198,373 - -2.7 198,518 - +0.3 198,767 - GFF3312 0.28 +0.1 198,767 - GFF3312 0.28 -0.1 198,866 - GFF3312 0.36 -0.1 198,904 + GFF3312 0.39 +0.4 198,904 + GFF3312 0.39 +0.7 198,905 - GFF3312 0.39 +0.1 199,160 - GFF3312 0.59 -0.7 199,199 - GFF3312 0.62 -0.3
Or see this region's nucleotide sequence