Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3303

Experiment: Ying_Nucleobase14 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3301 and GFF3302 are separated by 11 nucleotidesGFF3302 and GFF3303 are separated by 14 nucleotidesGFF3303 and GFF3304 are separated by 69 nucleotides GFF3301 - Cytosine deaminase (EC 3.5.4.1), at 185,522 to 185,983 GFF3301 GFF3302 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 185,995 to 186,762 GFF3302 GFF3303 - Hydroxymethylpyrimidine ABC transporter, ATPase component, at 186,777 to 187,607 GFF3303 GFF3304 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 187,677 to 188,711 GFF3304 Position (kb) 186 187 188Strain fitness (log2 ratio) -1 0 1at 185.794 kb on - strand, within GFF3301at 185.805 kb on + strand, within GFF3301at 186.062 kb on + strandat 186.239 kb on + strand, within GFF3302at 186.862 kb on + strand, within GFF3303at 186.862 kb on + strand, within GFF3303at 186.863 kb on - strand, within GFF3303at 187.228 kb on + strand, within GFF3303at 187.549 kb on + strandat 187.679 kb on - strandat 188.063 kb on - strand, within GFF3304at 188.077 kb on + strand, within GFF3304at 188.078 kb on - strand, within GFF3304at 188.380 kb on + strand, within GFF3304at 188.381 kb on - strand, within GFF3304at 188.473 kb on + strand, within GFF3304at 188.474 kb on - strand, within GFF3304at 188.474 kb on - strand, within GFF3304at 188.557 kb on + strand, within GFF3304at 188.558 kb on - strand, within GFF3304

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 2
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185,794 - GFF3301 0.59 +0.1
185,805 + GFF3301 0.61 +0.4
186,062 + -0.8
186,239 + GFF3302 0.32 -1.5
186,862 + GFF3303 0.10 -0.9
186,862 + GFF3303 0.10 -0.7
186,863 - GFF3303 0.10 +0.5
187,228 + GFF3303 0.54 -0.3
187,549 + -0.3
187,679 - +1.0
188,063 - GFF3304 0.37 -0.1
188,077 + GFF3304 0.39 -0.1
188,078 - GFF3304 0.39 -0.1
188,380 + GFF3304 0.68 +0.5
188,381 - GFF3304 0.68 +0.2
188,473 + GFF3304 0.77 -0.4
188,474 - GFF3304 0.77 +0.7
188,474 - GFF3304 0.77 -0.4
188,557 + GFF3304 0.85 -0.9
188,558 - GFF3304 0.85 -1.0

Or see this region's nucleotide sequence